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The map label for this gene is queA

Identifier: 188527195

GI number: 188527195

Start: 382684

End: 383721

Strand: Reverse

Name: queA

Synonym: HPSH_02010

Alternate gene names: 188527195

Gene position: 383721-382684 (Counterclockwise)

Preceding gene: 188527196

Following gene: 188527194

Centisome position: 23.86

GC content: 38.25

Gene sequence:

>1038_bases
TTGAAAGAATTTGATTTAGAAAGCTATGATTATGATTTGCCTAAGGAATTGATCGCAAACTACCCCATTTTGCCCAAAGA
AAAGGCTAAATTACTCGTATATGAAAGGCGTTCGCAAAAAATCACGCACACCACTTTTGAGCATGTTTTAGATTTTTTCC
CTAAAAACGCCCTTGTGGTGTTGAACGACACTAAAGTGATTAAGGCTAGGATTTTTGGATCTAAGCATGCCTTTTTGCCA
TCAAAAACAACCGAAGTGTTTTTCCACCGCTTCTTTAAAGATAATACCGCTCTAACTCAAATCAAGGGCAAGATCAAAGT
GGGGGATAAAATCTTTTTTGACGCACATTACCACGCTGAAGTTTTGGAATTGCTTCATAACGGCCAGCGCTTGATCGCTT
TTTATGGCAATAAAACCCCCTTAAATCAAGCCAATATCTTAAAACTTTTAGAGCAATACGGGCATATGCCCTTACCCCCT
TATATTAAAAGAGCGGATGAAAGTTTGGATGCGCATGAATACCAAAGCGTGTTCGCTAAACACATGGGTGCGGTGGCTGC
CCCTACAGCGTCATTGCATTTTTCTCAAAATACCTTAGAAAAATTATTGAAAGATTTCAAGCACGCTTTTTTAACCTTGC
ATGTGGGGGCTGGGACTTTTCTTGGTGTGGAAACTAAGGATATTAGAGAGCATCAAATCCATACAGAAGTTTTACGCATT
CCTAAAAAGAGCCAAGAAATTTTGCAAAAATCCCAAGAGATTTTATGCATCGGTACGACCGCTTTAAGGAGCGTGGAATA
CTTTAAGCGTTTAGAAAACCCTAATCAAGAGGCGTTTGAATGCGATATATTTTTGCATCTTGCTAATCCTATTTTGCATG
TTAATTATTTGCTCACCAATTTCCATTTGCCCAAATCAAGCCTTTTAATGCTTGTAAGCGCGATGGTAGGCTTAGAAAAA
ACCAAAGAAATCTATCAAATAGCCATAGAAAAGAAGTATCGTTTTTATTCTTATGGCGATGGGATGCTGATTTTATGA

Upstream 100 bases:

>100_bases
GATCGCCAAAATAGTCAATCCGTCTCCCAAAGACAACGAAAATAACAACGAAGAATACAACGAAAATAGCGCCAAAGAAA
ATGAAAAAAGCGAGTCGTAG

Downstream 100 bases:

>100_bases
ACCCCTTATTGCAAGATTATGCGCGCATCCTTTTAGAATGGAATCAAACGCACAACTTGAGCGGCGCGAAACATTTAAGC
GAGTTAGAACCCCAGATCAC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKEFDLESYDYDLPKELIANYPILPKEKAKLLVYERRSQKITHTTFEHVLDFFPKNALVVLNDTKVIKARIFGSKHAFLP
SKTTEVFFHRFFKDNTALTQIKGKIKVGDKIFFDAHYHAEVLELLHNGQRLIAFYGNKTPLNQANILKLLEQYGHMPLPP
YIKRADESLDAHEYQSVFAKHMGAVAAPTASLHFSQNTLEKLLKDFKHAFLTLHVGAGTFLGVETKDIREHQIHTEVLRI
PKKSQEILQKSQEILCIGTTALRSVEYFKRLENPNQEAFECDIFLHLANPILHVNYLLTNFHLPKSSLLMLVSAMVGLEK
TKEIYQIAIEKKYRFYSYGDGMLIL

Sequences:

>Translated_345_residues
MKEFDLESYDYDLPKELIANYPILPKEKAKLLVYERRSQKITHTTFEHVLDFFPKNALVVLNDTKVIKARIFGSKHAFLP
SKTTEVFFHRFFKDNTALTQIKGKIKVGDKIFFDAHYHAEVLELLHNGQRLIAFYGNKTPLNQANILKLLEQYGHMPLPP
YIKRADESLDAHEYQSVFAKHMGAVAAPTASLHFSQNTLEKLLKDFKHAFLTLHVGAGTFLGVETKDIREHQIHTEVLRI
PKKSQEILQKSQEILCIGTTALRSVEYFKRLENPNQEAFECDIFLHLANPILHVNYLLTNFHLPKSSLLMLVSAMVGLEK
TKEIYQIAIEKKYRFYSYGDGMLIL
>Mature_345_residues
MKEFDLESYDYDLPKELIANYPILPKEKAKLLVYERRSQKITHTTFEHVLDFFPKNALVVLNDTKVIKARIFGSKHAFLP
SKTTEVFFHRFFKDNTALTQIKGKIKVGDKIFFDAHYHAEVLELLHNGQRLIAFYGNKTPLNQANILKLLEQYGHMPLPP
YIKRADESLDAHEYQSVFAKHMGAVAAPTASLHFSQNTLEKLLKDFKHAFLTLHVGAGTFLGVETKDIREHQIHTEVLRI
PKKSQEILQKSQEILCIGTTALRSVEYFKRLENPNQEAFECDIFLHLANPILHVNYLLTNFHLPKSSLLMLVSAMVGLEK
TKEIYQIAIEKKYRFYSYGDGMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=355, Percent_Identity=36.9014084507042, Blast_Score=218, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39835; Mature: 39835

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFDLESYDYDLPKELIANYPILPKEKAKLLVYERRSQKITHTTFEHVLDFFPKNALVV
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
LNDTKVIKARIFGSKHAFLPSKTTEVFFHRFFKDNTALTQIKGKIKVGDKIFFDAHYHAE
EECCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEECEEEECCEEEEECHHHHH
VLELLHNGQRLIAFYGNKTPLNQANILKLLEQYGHMPLPPYIKRADESLDAHEYQSVFAK
HHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHH
HMGAVAAPTASLHFSQNTLEKLLKDFKHAFLTLHVGAGTFLGVETKDIREHQIHTEVLRI
HCCCCCCCCHHEEECHHHHHHHHHHHHHHEEEEEECCCEEECCCCHHHHHHHHHHHHHHC
PKKSQEILQKSQEILCIGTTALRSVEYFKRLENPNQEAFECDIFLHLANPILHVNYLLTN
CCHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHH
FHLPKSSLLMLVSAMVGLEKTKEIYQIAIEKKYRFYSYGDGMLIL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEC
>Mature Secondary Structure
MKEFDLESYDYDLPKELIANYPILPKEKAKLLVYERRSQKITHTTFEHVLDFFPKNALVV
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
LNDTKVIKARIFGSKHAFLPSKTTEVFFHRFFKDNTALTQIKGKIKVGDKIFFDAHYHAE
EECCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEECEEEECCEEEEECHHHHH
VLELLHNGQRLIAFYGNKTPLNQANILKLLEQYGHMPLPPYIKRADESLDAHEYQSVFAK
HHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHH
HMGAVAAPTASLHFSQNTLEKLLKDFKHAFLTLHVGAGTFLGVETKDIREHQIHTEVLRI
HCCCCCCCCHHEEECHHHHHHHHHHHHHHEEEEEECCCEEECCCCHHHHHHHHHHHHHHC
PKKSQEILQKSQEILCIGTTALRSVEYFKRLENPNQEAFECDIFLHLANPILHVNYLLTN
CCHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHH
FHLPKSSLLMLVSAMVGLEKTKEIYQIAIEKKYRFYSYGDGMLIL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA