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The map label for this gene is gidB

Identifier: 188527194

GI number: 188527194

Start: 382151

End: 382687

Strand: Reverse

Name: gidB

Synonym: HPSH_02005

Alternate gene names: 188527194

Gene position: 382687-382151 (Counterclockwise)

Preceding gene: 188527195

Following gene: 188527193

Centisome position: 23.79

GC content: 35.94

Gene sequence:

>537_bases
ATGAACCCCTTATTGCAAGATTATGCGCGCATCCTTTTAGAATGGAATCAAACGCACAACTTGAGCGGCGCGAAACATTT
AAGCGAGTTAGAACCCCAGATCACAGACGCTCTAAAACCTTTAGAATTTATCAAAGATTTTAAAAGTTGTTTGGATATTG
GGAGCGGGGCGGGACTTCCTGCTATCCCTTTAGCCCTTGAAAAACCTGAAGTCAAATTCATTCTTTTAGAGCCAAGAATA
AAAAGAGCGGCTTTTTTAAGCTACCTTAAAAGCGTTTTGCCTTTAAAAAATATTGAAATTGTTAAAAAGCGTTTAGAAGA
TTACCAAAATCTTTTACAAGTGGATTTAATCACTTCCAGAGCGGTCGCTAGCTCTTCTTTTTTGATAGAAAAAAGCCAAC
GCTTCCTAAAAGATAAGGGGTATTTTTTATTTTATAAAGGCGAGCAGCTAAAAGGTGAAATCGCTTGTAAAGACACTGAA
TGCTTTATGCATCAAAAGCGTATTTATTTTTACAAATCAAAGGAAAGTTTATGTTAA

Upstream 100 bases:

>100_bases
TAAGCGCGATGGTAGGCTTAGAAAAAACCAAAGAAATCTATCAAATAGCCATAGAAAAGAAGTATCGTTTTTATTCTTAT
GGCGATGGGATGCTGATTTT

Downstream 100 bases:

>100_bases
GAATTTTAATCCCCCTACTCATTATTGTGTGGGTTTTATGGCGTTTGTTTTTGAGGCAAAAACCCCTTAAAGACAACCAC
TCTTATACGCAACAAACCCC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MNPLLQDYARILLEWNQTHNLSGAKHLSELEPQITDALKPLEFIKDFKSCLDIGSGAGLPAIPLALEKPEVKFILLEPRI
KRAAFLSYLKSVLPLKNIEIVKKRLEDYQNLLQVDLITSRAVASSSFLIEKSQRFLKDKGYFLFYKGEQLKGEIACKDTE
CFMHQKRIYFYKSKESLC

Sequences:

>Translated_178_residues
MNPLLQDYARILLEWNQTHNLSGAKHLSELEPQITDALKPLEFIKDFKSCLDIGSGAGLPAIPLALEKPEVKFILLEPRI
KRAAFLSYLKSVLPLKNIEIVKKRLEDYQNLLQVDLITSRAVASSSFLIEKSQRFLKDKGYFLFYKGEQLKGEIACKDTE
CFMHQKRIYFYKSKESLC
>Mature_178_residues
MNPLLQDYARILLEWNQTHNLSGAKHLSELEPQITDALKPLEFIKDFKSCLDIGSGAGLPAIPLALEKPEVKFILLEPRI
KRAAFLSYLKSVLPLKNIEIVKKRLEDYQNLLQVDLITSRAVASSSFLIEKSQRFLKDKGYFLFYKGEQLKGEIACKDTE
CFMHQKRIYFYKSKESLC

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=149, Percent_Identity=32.8859060402685, Blast_Score=84, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 20515; Mature: 20515

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLLQDYARILLEWNQTHNLSGAKHLSELEPQITDALKPLEFIKDFKSCLDIGSGAGLP
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AIPLALEKPEVKFILLEPRIKRAAFLSYLKSVLPLKNIEIVKKRLEDYQNLLQVDLITSR
CCCEEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AVASSSFLIEKSQRFLKDKGYFLFYKGEQLKGEIACKDTECFMHQKRIYFYKSKESLC
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECHHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure
MNPLLQDYARILLEWNQTHNLSGAKHLSELEPQITDALKPLEFIKDFKSCLDIGSGAGLP
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AIPLALEKPEVKFILLEPRIKRAAFLSYLKSVLPLKNIEIVKKRLEDYQNLLQVDLITSR
CCCEEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AVASSSFLIEKSQRFLKDKGYFLFYKGEQLKGEIACKDTECFMHQKRIYFYKSKESLC
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECHHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]