Click here to switch to the map view.

The map label for this gene is pyrG

Identifier: 188527154

GI number: 188527154

Start: 339142

End: 340758

Strand: Reverse

Name: pyrG

Synonym: HPSH_01805

Alternate gene names: 188527154

Gene position: 340758-339142 (Counterclockwise)

Preceding gene: 188527163

Following gene: 188527153

Centisome position: 21.18

GC content: 42.18

Gene sequence:

>1617_bases
ATGGATAGAGCCAAATTTATATTCGTTACAGGGGGCGTGTTAAGCTCTCTAGGGAAAGGGATTTCATCTTCTTCAATCGC
TACGCTTTTACAGCATTGCAATTATCAGGTTTCTATTTTGAAGATTGACCCTTATATCAATATTGATCCAGGCACCATGA
GCCCTTTAGAGCATGGGGAAGTGTTTGTAACTAGCGATGGCGCTGAAACAGATTTAGACATAGGGCATTATGAACGCTTT
TTGAACAGGAATTTAACAAGGTTGAACAATTTCACTACCGGGCAGATTTTTTCAAGCGTGATAGAAAATGAAAGGAAAGG
GGAATATTTAGGCAAAACCATTCAAATCGTCCCCCATGTAACCGATGAAATCAAAAGGCGCATTAAAAGCGCGGCTAAGG
GGTTGGATTTTTTAATCGTGGAAGTGGGCGGAACCGTGGGCGATATGGAGGGCATGTTTTATTTGGAAGCGATCCGCCAG
CTTAAATTGGAATTAGGGAATGAAAAAGTCATCAATGTGCATGTAACCTTGATCCCCTATATCCAAACCACTAACGAATT
AAAAACCAAACCCACGCAACACTCCGTCCAAGAATTACGGCGCCTTGGCGTAACCCCTCAAATCATTTTGGCGCGATCGC
CTAAGCCTTTGGATAAAGAATTGAAAAATAAAATCGCTTTGAGTTGCGATGTGGAGCGAGACAGCGTGATTGTCGCCACA
GACACTAAAAGCATTTACGCATGCCCTATTCTTTTCTTGCAAGAAGGCATTTTAACCCCCATTGCCAGACGCTTTAATTT
GAATAAACTACACCCCAAAATGGCGGCTTGGAACACTTTAGTAGAAAAGATTATCGCTCCTAAACACAAAGTCAAAATTG
GTTTTGTGGGCAAGTATTTAAGCTTAAAAGAATCTTATAAATCCTTGATTGAAGCCTTAATCCATGCGGGCGCGCATTTG
GATGTGCAAGTCAATATTGAATGGCTAGATAGCGAGAATTTTAACGAAAAGACCGATTTAGAGGGCGTTGATGCGATTTT
AGTGCCTGGGGGCTTTGGAGAAAGGGGGATTGAAGGCAAAATTTGCGCCATTCAAAGGGCAAGGTTAGAAAAACTCCCCT
TTTTAGGGATTTGTTTGGGCATGCAATTAGCGATCGTTGAATTTTGCCGCAATGTTTTGGGTTTAAAAGGGGCTAACTCT
ACGGAGTTTAACCAACGCTGCGAATACCCTGTGGTGTATTTGATTGAAGATTTTATCGATCAAAACCACCAAAAACAGGT
GCGCACCTATAATTCGCCTTTAGGAGGCACCATGCGATTAGGCGAATACGAATGCGAAATCGTGCCAAATAGCTTGTTAG
AAAAAGCTTATAAAAAGCCTAGCATTAAAGAAAGGCACCGCCATCGTTATGAAATCAACCCCAAATACCGCCAAGAGTGG
GAAAATAAGGGCTTGAAAGTGGTGGGTTTTGGAGCGAATCATTTGATAGAAGCGATTGAGTTAGAAGATCACCCGTTCTT
TGTGGGGGTGCAATTCCACCCGGAATTCACCTCCAGGTTGCAAAGCCCTAACCCTATTATTTTGGATTTCATTAAGAGCG
CTCTTCATAAATCCTAA

Upstream 100 bases:

>100_bases
TTTTTTTTGAAATCGCTTTAATTTTTGCCCTCTTTTAAAGCTTTTTAACCCTTTTTAGACTACAATGCGAGAAGTAAAAA
TCAAAGGAACGCAATAACTT

Downstream 100 bases:

>100_bases
TCTAAAAAATGAAACAAAAGCTTAAAGCTCAAATCAAAGAGCGAGTGGCTTCTATCGCTTATAATGAAAAAGGGTTTCCT
AGCCCCTTTTTATTTAAAGA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGEVFVTSDGAETDLDIGHYERF
LNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHVTDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQ
LKLELGNEKVINVHVTLIPYIQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVERDSVIVAT
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYLSLKESYKSLIEALIHAGAHL
DVQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGKICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANS
TEFNQRCEYPVVYLIEDFIDQNHQKQVRTYNSPLGGTMRLGEYECEIVPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
ENKGLKVVGFGANHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALHKS

Sequences:

>Translated_538_residues
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGEVFVTSDGAETDLDIGHYERF
LNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHVTDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQ
LKLELGNEKVINVHVTLIPYIQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVERDSVIVAT
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYLSLKESYKSLIEALIHAGAHL
DVQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGKICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANS
TEFNQRCEYPVVYLIEDFIDQNHQKQVRTYNSPLGGTMRLGEYECEIVPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
ENKGLKVVGFGANHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALHKS
>Mature_538_residues
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGEVFVTSDGAETDLDIGHYERF
LNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHVTDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQ
LKLELGNEKVINVHVTLIPYIQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVERDSVIVAT
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYLSLKESYKSLIEALIHAGAHL
DVQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGKICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANS
TEFNQRCEYPVVYLIEDFIDQNHQKQVRTYNSPLGGTMRLGEYECEIVPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
ENKGLKVVGFGANHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALHKS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=548, Percent_Identity=45.6204379562044, Blast_Score=469, Evalue=1e-132,
Organism=Homo sapiens, GI28559085, Length=557, Percent_Identity=43.4470377019749, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI28559083, Length=557, Percent_Identity=43.4470377019749, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI221316689, Length=557, Percent_Identity=43.4470377019749, Blast_Score=452, Evalue=1e-127,
Organism=Escherichia coli, GI1789142, Length=532, Percent_Identity=49.4360902255639, Blast_Score=542, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=38.423645320197, Blast_Score=418, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322563, Length=571, Percent_Identity=41.5061295971979, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319432, Length=571, Percent_Identity=40.4553415061296, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=43.9285714285714, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=41.980198019802, Blast_Score=371, Evalue=1e-103,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60699; Mature: 60699

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGE
CCCCEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCCCCCCC
VFVTSDGAETDLDIGHYERFLNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHV
EEEECCCCCCCCCHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCHHCCEEEEECCC
TDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQLKLELGNEKVINVHVTLIPY
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEE
IQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVERDSVIVAT
EECCCHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCEEEEEECCCCCEEEEE
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYL
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHH
SLKESYKSLIEALIHAGAHLDVQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGK
HHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
ICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANSTEFNQRCEYPVVYLIEDFID
EEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHC
QNHQKQVRTYNSPLGGTMRLGEYECEIVPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
CCCHHHHHHCCCCCCCCEEECCEEEEECCHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHH
ENKGLKVVGFGANHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALHKS
HHCCCEEEEECHHHHHEEEEECCCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGE
CCCCEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCCCCCCC
VFVTSDGAETDLDIGHYERFLNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHV
EEEECCCCCCCCCHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCHHCCEEEEECCC
TDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQLKLELGNEKVINVHVTLIPY
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEE
IQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVERDSVIVAT
EECCCHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCEEEEEECCCCCEEEEE
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYL
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHH
SLKESYKSLIEALIHAGAHLDVQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGK
HHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
ICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANSTEFNQRCEYPVVYLIEDFID
EEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHC
QNHQKQVRTYNSPLGGTMRLGEYECEIVPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
CCCHHHHHHCCCCCCCCEEECCEEEEECCHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHH
ENKGLKVVGFGANHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALHKS
HHCCCEEEEECHHHHHEEEEECCCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA