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The map label for this gene is recJ [H]

Identifier: 188527153

GI number: 188527153

Start: 337583

End: 339133

Strand: Reverse

Name: recJ [H]

Synonym: HPSH_01800

Alternate gene names: 188527153

Gene position: 339133-337583 (Counterclockwise)

Preceding gene: 188527154

Following gene: 188527152

Centisome position: 21.08

GC content: 40.43

Gene sequence:

>1551_bases
ATGAAACAAAAGCTTAAAGCTCAAATCAAAGAGCGAGTGGCTTCTATCGCTTATAATGAAAAAGGGTTTCCTAGCCCCTT
TTTATTTAAAGACTTGAAAAAAGCCGCGCTCAAAATCATAGAAGCCATGCGCGCAAACACAGAGATTTTAGTGGTGGGCG
ATTATGACGCTGACGGCGTGATTAGCTCTACTATCATGGCAAAATTCTTTGAAAGCCTGAACTATAAGCATGTTCGCATT
GTAATCCCTAATCGCTTCATTGATGGCTATGGGATTTCTAAAAAATTTTTAGAAAAACACCACGCCCCTTTAATCATCAC
GGTGGATAACGGGATTAGCGCCTTTGAAGCCACGCGATTTTGTAAAGAAAAAAATTACACCCTTATCATCACAGATCACC
ATTGCTTACACCATGATGAAGTCCCAGACGCTTATGCGGTGATCAACCCCAAGCAACCGGATTGTGATTTTATCCAAAAG
GAAGTGTGCGGGGCGTTGGTGGCGTTTTATTTGTGCTATGGCATCCACCAGCTTTTAGGAAAAGAAAAAAGCCATTCTAG
CGAGTTGTTGTGTTTGGCGGGCGTGGCGACTATTGCTGACATGATGCCTTTGACTTTTTTTAACCGCTTTTTAGTTTCTA
AAGCCTTGTATTTTTTGCAAAAAGAATCCTTAGGAGCGATGGGGTTTTTGCGCCAAAGAGAAGTTTTTAGAAAACGCTCT
TTAAAAGCGAGCGATATTTCTTTTAGTATCGCCCCCTTAATCAACTCCGCAGGGCGCATGCAAGATGCGAAAATGGCTTT
AGATTTTTTAAGCGCGAATAATTTTCAAGATGGCTATTTTTTGTATGAACGCTTGAAAGCATGCAATATGAAGCGTAAAA
TGATCCAACAGCAGGTTTTTGAAGAGGCTCTTAAGCATGCGATGGTTGGCGAAAAAATTATCGTCGCTTTTAAGGATGAT
TGGCATGAGGGTGTGCTTGGGATTGTGGCTTCAAAATTAGTGGAAGCCACTCAAAAGCCAAGCCTGGTTTTTACCTTTAA
AGAAGGGGTGTATAAAGGGAGCGGGCGCAGCTCTTCAAATATTGATCTCATTGACGCTTTGAATGGGGTTTCTTCTTTGT
TGCTCGGCTATGGGGGGCATAGGCAAGCTTGCGGTTTGAGCGTTGGAAAAAACAATATCATCTCGCTCTTTGAAACTTTA
GAAAATTTTGATTTTAAAATCCTACCTTTTTGTGAAAAAGAACCCCCTTTAACGCTCACATTAAAAGACATTGATAGAGA
GCTTTTAGAGATTATAGAAATGGGCGAACCTTATGGGCAAGAAAACCCAGAGCCGCTCTTTCAAGCGCAAAATTTAGAAG
TCATAGAAGAAAAAATCATTAAAGAAAGCCACCAGGTTTTGCGTTTTAAGGATAGAGAATGCATCAAAGAGGCTATTTAT
TTTAACACTGAGCGGTTTTTGAAAGCGGGCGAAAAGGTGAATGCGATTTTTAGCGTGGAATTAGATGAGTGTTCTAATGA
GCCTAAAATGTTTGTTAAAAGTTTGTTGTAG

Upstream 100 bases:

>100_bases
GGGTGCAATTCCACCCGGAATTCACCTCCAGGTTGCAAAGCCCTAACCCTATTATTTTGGATTTCATTAAGAGCGCTCTT
CATAAATCCTAATCTAAAAA

Downstream 100 bases:

>100_bases
TGCCTTTTGTTGAAGAAGAATTTGAAATTTTAAAACCCACCAAAGCCTTGTTTTTTGTGTGCGATATTTTAAAATGCTCT
TTAAAAGAAGCCCAGCGCCA

Product: single-stranded-DNA specific exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MKQKLKAQIKERVASIAYNEKGFPSPFLFKDLKKAALKIIEAMRANTEILVVGDYDADGVISSTIMAKFFESLNYKHVRI
VIPNRFIDGYGISKKFLEKHHAPLIITVDNGISAFEATRFCKEKNYTLIITDHHCLHHDEVPDAYAVINPKQPDCDFIQK
EVCGALVAFYLCYGIHQLLGKEKSHSSELLCLAGVATIADMMPLTFFNRFLVSKALYFLQKESLGAMGFLRQREVFRKRS
LKASDISFSIAPLINSAGRMQDAKMALDFLSANNFQDGYFLYERLKACNMKRKMIQQQVFEEALKHAMVGEKIIVAFKDD
WHEGVLGIVASKLVEATQKPSLVFTFKEGVYKGSGRSSSNIDLIDALNGVSSLLLGYGGHRQACGLSVGKNNIISLFETL
ENFDFKILPFCEKEPPLTLTLKDIDRELLEIIEMGEPYGQENPEPLFQAQNLEVIEEKIIKESHQVLRFKDRECIKEAIY
FNTERFLKAGEKVNAIFSVELDECSNEPKMFVKSLL

Sequences:

>Translated_516_residues
MKQKLKAQIKERVASIAYNEKGFPSPFLFKDLKKAALKIIEAMRANTEILVVGDYDADGVISSTIMAKFFESLNYKHVRI
VIPNRFIDGYGISKKFLEKHHAPLIITVDNGISAFEATRFCKEKNYTLIITDHHCLHHDEVPDAYAVINPKQPDCDFIQK
EVCGALVAFYLCYGIHQLLGKEKSHSSELLCLAGVATIADMMPLTFFNRFLVSKALYFLQKESLGAMGFLRQREVFRKRS
LKASDISFSIAPLINSAGRMQDAKMALDFLSANNFQDGYFLYERLKACNMKRKMIQQQVFEEALKHAMVGEKIIVAFKDD
WHEGVLGIVASKLVEATQKPSLVFTFKEGVYKGSGRSSSNIDLIDALNGVSSLLLGYGGHRQACGLSVGKNNIISLFETL
ENFDFKILPFCEKEPPLTLTLKDIDRELLEIIEMGEPYGQENPEPLFQAQNLEVIEEKIIKESHQVLRFKDRECIKEAIY
FNTERFLKAGEKVNAIFSVELDECSNEPKMFVKSLL
>Mature_516_residues
MKQKLKAQIKERVASIAYNEKGFPSPFLFKDLKKAALKIIEAMRANTEILVVGDYDADGVISSTIMAKFFESLNYKHVRI
VIPNRFIDGYGISKKFLEKHHAPLIITVDNGISAFEATRFCKEKNYTLIITDHHCLHHDEVPDAYAVINPKQPDCDFIQK
EVCGALVAFYLCYGIHQLLGKEKSHSSELLCLAGVATIADMMPLTFFNRFLVSKALYFLQKESLGAMGFLRQREVFRKRS
LKASDISFSIAPLINSAGRMQDAKMALDFLSANNFQDGYFLYERLKACNMKRKMIQQQVFEEALKHAMVGEKIIVAFKDD
WHEGVLGIVASKLVEATQKPSLVFTFKEGVYKGSGRSSSNIDLIDALNGVSSLLLGYGGHRQACGLSVGKNNIISLFETL
ENFDFKILPFCEKEPPLTLTLKDIDRELLEIIEMGEPYGQENPEPLFQAQNLEVIEEKIIKESHQVLRFKDRECIKEAIY
FNTERFLKAGEKVNAIFSVELDECSNEPKMFVKSLL

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=470, Percent_Identity=31.7021276595745, Blast_Score=225, Evalue=5e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 58440; Mature: 58440

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKLKAQIKERVASIAYNEKGFPSPFLFKDLKKAALKIIEAMRANTEILVVGDYDADGV
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHH
ISSTIMAKFFESLNYKHVRIVIPNRFIDGYGISKKFLEKHHAPLIITVDNGISAFEATRF
HHHHHHHHHHHHCCCCEEEEEECCCEECCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHH
CKEKNYTLIITDHHCLHHDEVPDAYAVINPKQPDCDFIQKEVCGALVAFYLCYGIHQLLG
HHCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
KEKSHSSELLCLAGVATIADMMPLTFFNRFLVSKALYFLQKESLGAMGFLRQREVFRKRS
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
LKASDISFSIAPLINSAGRMQDAKMALDFLSANNFQDGYFLYERLKACNMKRKMIQQQVF
CCCCCCCEEHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EEALKHAMVGEKIIVAFKDDWHEGVLGIVASKLVEATQKPSLVFTFKEGVYKGSGRSSSN
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
IDLIDALNGVSSLLLGYGGHRQACGLSVGKNNIISLFETLENFDFKILPFCEKEPPLTLT
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEE
LKDIDRELLEIIEMGEPYGQENPEPLFQAQNLEVIEEKIIKESHQVLRFKDRECIKEAIY
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNTERFLKAGEKVNAIFSVELDECSNEPKMFVKSLL
CCHHHHHHCCCCCCEEEEEEHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MKQKLKAQIKERVASIAYNEKGFPSPFLFKDLKKAALKIIEAMRANTEILVVGDYDADGV
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHH
ISSTIMAKFFESLNYKHVRIVIPNRFIDGYGISKKFLEKHHAPLIITVDNGISAFEATRF
HHHHHHHHHHHHCCCCEEEEEECCCEECCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHH
CKEKNYTLIITDHHCLHHDEVPDAYAVINPKQPDCDFIQKEVCGALVAFYLCYGIHQLLG
HHCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
KEKSHSSELLCLAGVATIADMMPLTFFNRFLVSKALYFLQKESLGAMGFLRQREVFRKRS
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
LKASDISFSIAPLINSAGRMQDAKMALDFLSANNFQDGYFLYERLKACNMKRKMIQQQVF
CCCCCCCEEHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EEALKHAMVGEKIIVAFKDDWHEGVLGIVASKLVEATQKPSLVFTFKEGVYKGSGRSSSN
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
IDLIDALNGVSSLLLGYGGHRQACGLSVGKNNIISLFETLENFDFKILPFCEKEPPLTLT
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEE
LKDIDRELLEIIEMGEPYGQENPEPLFQAQNLEVIEEKIIKESHQVLRFKDRECIKEAIY
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNTERFLKAGEKVNAIFSVELDECSNEPKMFVKSLL
CCHHHHHHCCCCCCEEEEEEHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1987126; 9278503; 2649886 [H]