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The map label for this gene is metK [H]

Identifier: 188527002

GI number: 188527002

Start: 188494

End: 189651

Strand: Direct

Name: metK [H]

Synonym: HPSH_01010

Alternate gene names: 188527002

Gene position: 188494-189651 (Clockwise)

Preceding gene: 188527001

Following gene: 188527003

Centisome position: 11.72

GC content: 44.21

Gene sequence:

>1158_bases
ATGAAAGATAGTTTTCTTTTCACTTCTGAATCAGTAACTGAGGGGCATCCTGACAAAATGGCTGATCAAATCAGCGATGC
GGTTTTAGATTACATTATTGAGCGCGATCAAAAAGCCAAAGTTGCATGCGAGACTTTAGTTGCTAATGGGTTTTGCATGA
TCACTGGCGAGTTAAAAACTTCTGTTTATGCCCCTATGCAAGAGATTGCAAGAGAAGTGGTTAAAAAGATTGGTTATACA
GACGCTCTTTATGGCTTTGATTATAGAAGCGCGGCGGTTTTAAATGGCGTTGGCGAGCAAAGCCCTGATATTAATCAAGG
CGTGGATAGAGAAGATGGCGAGATTGGGGCAGGGGATCAAGGGCTTATGTTTGGTTATGCATGCAAGGAGACTGAAACGC
TCATGCCCTTACCCATTCATTTAGCGCACCAGCTCACTTTCGCTCTGGCTCAAAAAAGAAAAGACAACACTCTGCCTTTT
TTAAGGCCTGATGGCAAGTCTCAAGTGAGCGTGCGTTATGAAAACAACAAGCCTGTAAGCATTGATACGATTGTCATTTC
CACCCAACATTCCCCAGAAGTTTCGCAAAAACATTTAAAAGAATCCGTGATTGAAGAGATCGTGTATAAGGTTTTACCCA
AAGAATATTTGCATGACAATATCAAGTTTTTTGTCAATCCTACAGGAAAATTCGTTATTGGTGGGCCGCAAGGCGATGCG
GGCTTGACGGGCAGAAAAATCATCGTGGATACTTATGGGGGGAGTTGCCCGCATGGCGGGGGAGCGTTTAGTGGGAAAGA
CCCTAGTAAAGTGGATAGGAGCGCGGCTTATGCGGCCCGCTATGTGGCTAAAAATTTGGTAGCGAGTGGGGTTTGCGATA
AAGCGACTGTGCAGCTTGCTTATGCGATTGGGGTGATAGAGCCGGTGTCTGTTTATGTGAATACGCATAACACGAGCAAG
TATTCAAGCGCGGAGTTGGAAAAATGCGTGAAATCGGTTTTCAAACTCACGCCAAAAGGCATTATTGAAAGCTTGGATCT
GTTAAGGCCCATTTATTCGCTCACTTCAGCTTATGGGCATTTTGGGCGAGAATTAGAGGAATTCACTTGGGAAAAAACCA
ACAAAGCCGAAGAGATTCAAGCGTTCTTTAAGCGTTAA

Upstream 100 bases:

>100_bases
TTGTTGCAAAAAGCTAAAGGGTTTTTTAAGTCTTAAAGAATGAAATAAGCTATAATAAGCCCATTTTGAATACGAATGAT
TATTTTAATAAGGACAATCA

Downstream 100 bases:

>100_bases
AAAAATGTTTTAAGGGTAATATTTTAAAAAATTTTTGTATAATCAACAATTCACAAGGAGTTTAAATTGAAACAAAGAAC
GCTGTCTATTATTAAACCGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDQKAKVACETLVANGFCMITGELKTSVYAPMQEIAREVVKKIGYT
DALYGFDYRSAAVLNGVGEQSPDINQGVDREDGEIGAGDQGLMFGYACKETETLMPLPIHLAHQLTFALAQKRKDNTLPF
LRPDGKSQVSVRYENNKPVSIDTIVISTQHSPEVSQKHLKESVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDKATVQLAYAIGVIEPVSVYVNTHNTSK
YSSAELEKCVKSVFKLTPKGIIESLDLLRPIYSLTSAYGHFGRELEEFTWEKTNKAEEIQAFFKR

Sequences:

>Translated_385_residues
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDQKAKVACETLVANGFCMITGELKTSVYAPMQEIAREVVKKIGYT
DALYGFDYRSAAVLNGVGEQSPDINQGVDREDGEIGAGDQGLMFGYACKETETLMPLPIHLAHQLTFALAQKRKDNTLPF
LRPDGKSQVSVRYENNKPVSIDTIVISTQHSPEVSQKHLKESVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDKATVQLAYAIGVIEPVSVYVNTHNTSK
YSSAELEKCVKSVFKLTPKGIIESLDLLRPIYSLTSAYGHFGRELEEFTWEKTNKAEEIQAFFKR
>Mature_385_residues
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDQKAKVACETLVANGFCMITGELKTSVYAPMQEIAREVVKKIGYT
DALYGFDYRSAAVLNGVGEQSPDINQGVDREDGEIGAGDQGLMFGYACKETETLMPLPIHLAHQLTFALAQKRKDNTLPF
LRPDGKSQVSVRYENNKPVSIDTIVISTQHSPEVSQKHLKESVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDKATVQLAYAIGVIEPVSVYVNTHNTSK
YSSAELEKCVKSVFKLTPKGIIESLDLLRPIYSLTSAYGHFGRELEEFTWEKTNKAEEIQAFFKR

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=378, Percent_Identity=54.2328042328042, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI4557737, Length=377, Percent_Identity=51.9893899204244, Blast_Score=380, Evalue=1e-105,
Organism=Escherichia coli, GI1789311, Length=376, Percent_Identity=61.7021276595745, Blast_Score=479, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17551082, Length=378, Percent_Identity=52.1164021164021, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=51.8421052631579, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17538494, Length=374, Percent_Identity=52.6737967914438, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17538498, Length=374, Percent_Identity=52.1390374331551, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI32565905, Length=374, Percent_Identity=48.3957219251337, Blast_Score=324, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI25145635, Length=329, Percent_Identity=49.5440729483283, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=49.6932515337423, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=51.1627906976744, Blast_Score=288, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=56.0975609756098, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320710, Length=380, Percent_Identity=53.9473684210526, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=52.6455026455027, Blast_Score=393, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=51.0526315789474, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=51.0526315789474, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=51.0526315789474, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=51.0526315789474, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=51.0526315789474, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=51.0526315789474, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=50.5263157894737, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=50.5263157894737, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580541, Length=366, Percent_Identity=49.1803278688525, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=49.5798319327731, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42359; Mature: 42359

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDQKAKVACETLVANGFCMITGELKT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEECHHH
SVYAPMQEIAREVVKKIGYTDALYGFDYRSAAVLNGVGEQSPDINQGVDREDGEIGAGDQ
HHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GLMFGYACKETETLMPLPIHLAHQLTFALAQKRKDNTLPFLRPDGKSQVSVRYENNKPVS
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCCCCEE
IDTIVISTQHSPEVSQKHLKESVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEEECCCCCC
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDKATVQLA
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
YAIGVIEPVSVYVNTHNTSKYSSAELEKCVKSVFKLTPKGIIESLDLLRPIYSLTSAYGH
HHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FGRELEEFTWEKTNKAEEIQAFFKR
HHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDQKAKVACETLVANGFCMITGELKT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEECHHH
SVYAPMQEIAREVVKKIGYTDALYGFDYRSAAVLNGVGEQSPDINQGVDREDGEIGAGDQ
HHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GLMFGYACKETETLMPLPIHLAHQLTFALAQKRKDNTLPFLRPDGKSQVSVRYENNKPVS
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCCCCEE
IDTIVISTQHSPEVSQKHLKESVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEEECCCCCC
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDKATVQLA
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
YAIGVIEPVSVYVNTHNTSKYSSAELEKCVKSVFKLTPKGIIESLDLLRPIYSLTSAYGH
HHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FGRELEEFTWEKTNKAEEIQAFFKR
HHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA