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The map label for this gene is citT [H]

Identifier: 188526947

GI number: 188526947

Start: 139712

End: 141160

Strand: Reverse

Name: citT [H]

Synonym: HPSH_00720

Alternate gene names: 188526947

Gene position: 141160-139712 (Counterclockwise)

Preceding gene: 188526957

Following gene: 188526946

Centisome position: 8.78

GC content: 42.37

Gene sequence:

>1449_bases
ATGACTAAACAAATTCTCTCAATCCTTGCCCCTTTTTTTATCGCAACGCTATTGTATTTTTTAGGCGCACCAGATGGGTT
AAACCCTAACGCATGGCTTTATTTTTGTATCTTCATAGGCATGATTATAGGGCTAGTTTTAGAGCCGGTGCCACCAGGTT
TAGTGGCATTGAGCGCGTTAGTGTTGTGCGTGGCGTTAAAAATTGGAGCGAGTGATAAAGTAGCGAGCGCTAATAAGGCT
ATTTCGTGGGGTTTGAGCGGGTATGCGAATAAAACGGTGTGGCTTGTGTTTGTCGCTTTTATCTTGGGTTTAGGGTATGA
AAAAAGCTTGTTAGGGAAACGGATCGCTCTTTTACTCATTAGGTTTTTAGGGCAAACCCCTTTAGGTTTAGGCTATGCGA
TTGGTTTGAGCGAATTGTGTCTGGCCCCCTTTATCCCTAGCAACTCCGCTAGAAGTGGGGGCATACTCTATCCAATCGTT
TCATCTATCCCGTCTTTAATGAGCTCTACTCCAAATGATAACCCTGACAAGATCGGCGCGTATTTGATGTGGGTCGCTTT
GGCTTCAACTTGCATCACTTCGTCCATGTTTTTAACCGCACTCGCTCCTAACCCCCTAGCAATGGAAATCGCTACCAAAA
TAGGCGTGAATGAAATCTCATGGTTTTCGTGGTTTTTAGCGTTCTTGCCTTGTGGGGTGGTTTTGATTTTGCTTGCGCCT
TTATTGGCGTATAAAACCTGCAAGCCCACCTTAAAAGGCTCAAAAGAAGTGAGTTTGTGGGCTAAAAAAGAATTAGAGGG
CATGGGGAGGTTTTCTTTAAAAGAAATTTTAATGCTCAGCCTCACTTTATTGGCTTTATTGGGTTGGATTTTTGGCAAAC
CTTTAGGCTTGCATGCGAGCGCGACGGCTTTGATTGTCATGGTTTTAATGGCGTTTTGTAAGATTGTAAGCTATGAAGAC
ATCATCAAAAACAAGAGCGCGTTCAACATTTTTTTATTGCTTGGATCGTTGCTCACGATGGCTGGCGGGCTTAAAAATGT
AGGGTTTTTAAATTTTATCAGCAATGCGGCTAAAAATTTTTTAGAGCATGCTAACTTGGATTCGTTAATAGCGGTCTTGT
TTATTATAGCTCTCTTTTATCTGTTGCATTATTTTTTCGCCAGCATCACCGCTCATGTGAGCGCGTTATTTGCTCTTTTT
GTAGGGATTGGTTCGCACATTCAAGGGGTCAATTTGCAAGAATTGAGCTTGTTTTTAATGTTCTCTTTAGGGATTATGGG
GATTTTAACCCCCTATGGCACAGGCCCATCCACCATTTATTACGGGAGCGGGTATATTTCAAGCAAGGATTTTTGGAAAT
GGGGGTTTATTTTTGGTATGGTGTATTTGATCGTGTTTTTAAGCGTGTGCGTGCCTTGGGTCAAACTCATCGCTTATAGG
TGGTTGTAG

Upstream 100 bases:

>100_bases
GCTTAATTTTTAATCTTTTTCTGTAACATTGTAATAACTCAATCCAATTTGAATGATATTTTTAAGCCAACCCACTACTA
TCTTTGGCTAAAGGCTAAAC

Downstream 100 bases:

>100_bases
CTGGAAACTTTACACAACGCCCTTTTAAAATGGTATGAAAAATTTGGGCGAAAGGATTTACCTTTTAGGAACTTAAAGGG
CATTAACGCTCCTTATGAAG

Product: C(4)-dicarboxylates and tricarboxylates/succinate antiporter

Products: NA

Alternate protein names: Citrate transporter; Citrate/succinate antiporter [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTKQILSILAPFFIATLLYFLGAPDGLNPNAWLYFCIFIGMIIGLVLEPVPPGLVALSALVLCVALKIGASDKVASANKA
ISWGLSGYANKTVWLVFVAFILGLGYEKSLLGKRIALLLIRFLGQTPLGLGYAIGLSELCLAPFIPSNSARSGGILYPIV
SSIPSLMSSTPNDNPDKIGAYLMWVALASTCITSSMFLTALAPNPLAMEIATKIGVNEISWFSWFLAFLPCGVVLILLAP
LLAYKTCKPTLKGSKEVSLWAKKELEGMGRFSLKEILMLSLTLLALLGWIFGKPLGLHASATALIVMVLMAFCKIVSYED
IIKNKSAFNIFLLLGSLLTMAGGLKNVGFLNFISNAAKNFLEHANLDSLIAVLFIIALFYLLHYFFASITAHVSALFALF
VGIGSHIQGVNLQELSLFLMFSLGIMGILTPYGTGPSTIYYGSGYISSKDFWKWGFIFGMVYLIVFLSVCVPWVKLIAYR
WL

Sequences:

>Translated_482_residues
MTKQILSILAPFFIATLLYFLGAPDGLNPNAWLYFCIFIGMIIGLVLEPVPPGLVALSALVLCVALKIGASDKVASANKA
ISWGLSGYANKTVWLVFVAFILGLGYEKSLLGKRIALLLIRFLGQTPLGLGYAIGLSELCLAPFIPSNSARSGGILYPIV
SSIPSLMSSTPNDNPDKIGAYLMWVALASTCITSSMFLTALAPNPLAMEIATKIGVNEISWFSWFLAFLPCGVVLILLAP
LLAYKTCKPTLKGSKEVSLWAKKELEGMGRFSLKEILMLSLTLLALLGWIFGKPLGLHASATALIVMVLMAFCKIVSYED
IIKNKSAFNIFLLLGSLLTMAGGLKNVGFLNFISNAAKNFLEHANLDSLIAVLFIIALFYLLHYFFASITAHVSALFALF
VGIGSHIQGVNLQELSLFLMFSLGIMGILTPYGTGPSTIYYGSGYISSKDFWKWGFIFGMVYLIVFLSVCVPWVKLIAYR
WL
>Mature_481_residues
TKQILSILAPFFIATLLYFLGAPDGLNPNAWLYFCIFIGMIIGLVLEPVPPGLVALSALVLCVALKIGASDKVASANKAI
SWGLSGYANKTVWLVFVAFILGLGYEKSLLGKRIALLLIRFLGQTPLGLGYAIGLSELCLAPFIPSNSARSGGILYPIVS
SIPSLMSSTPNDNPDKIGAYLMWVALASTCITSSMFLTALAPNPLAMEIATKIGVNEISWFSWFLAFLPCGVVLILLAPL
LAYKTCKPTLKGSKEVSLWAKKELEGMGRFSLKEILMLSLTLLALLGWIFGKPLGLHASATALIVMVLMAFCKIVSYEDI
IKNKSAFNIFLLLGSLLTMAGGLKNVGFLNFISNAAKNFLEHANLDSLIAVLFIIALFYLLHYFFASITAHVSALFALFV
GIGSHIQGVNLQELSLFLMFSLGIMGILTPYGTGPSTIYYGSGYISSKDFWKWGFIFGMVYLIVFLSVCVPWVKLIAYRW
L

Specific function: Responsible for the uptake of citrate in exchange to the efflux of succinate. Has a relatively broad specificity for C(4)- dicarboxylates and tricarboxylates [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family. SODIT1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786829, Length=479, Percent_Identity=41.544885177453, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1789444, Length=478, Percent_Identity=40.3765690376569, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1786986, Length=463, Percent_Identity=35.85313174946, Blast_Score=258, Evalue=6e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 52349; Mature: 52217

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQILSILAPFFIATLLYFLGAPDGLNPNAWLYFCIFIGMIIGLVLEPVPPGLVALSAL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VLCVALKIGASDKVASANKAISWGLSGYANKTVWLVFVAFILGLGYEKSLLGKRIALLLI
HHHHHHHCCCCCCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RFLGQTPLGLGYAIGLSELCLAPFIPSNSARSGGILYPIVSSIPSLMSSTPNDNPDKIGA
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
YLMWVALASTCITSSMFLTALAPNPLAMEIATKIGVNEISWFSWFLAFLPCGVVLILLAP
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LLAYKTCKPTLKGSKEVSLWAKKELEGMGRFSLKEILMLSLTLLALLGWIFGKPLGLHAS
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
ATALIVMVLMAFCKIVSYEDIIKNKSAFNIFLLLGSLLTMAGGLKNVGFLNFISNAAKNF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LEHANLDSLIAVLFIIALFYLLHYFFASITAHVSALFALFVGIGSHIQGVNLQELSLFLM
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
FSLGIMGILTPYGTGPSTIYYGSGYISSKDFWKWGFIFGMVYLIVFLSVCVPWVKLIAYR
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WL
CC
>Mature Secondary Structure 
TKQILSILAPFFIATLLYFLGAPDGLNPNAWLYFCIFIGMIIGLVLEPVPPGLVALSAL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VLCVALKIGASDKVASANKAISWGLSGYANKTVWLVFVAFILGLGYEKSLLGKRIALLLI
HHHHHHHCCCCCCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RFLGQTPLGLGYAIGLSELCLAPFIPSNSARSGGILYPIVSSIPSLMSSTPNDNPDKIGA
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
YLMWVALASTCITSSMFLTALAPNPLAMEIATKIGVNEISWFSWFLAFLPCGVVLILLAP
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LLAYKTCKPTLKGSKEVSLWAKKELEGMGRFSLKEILMLSLTLLALLGWIFGKPLGLHAS
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
ATALIVMVLMAFCKIVSYEDIIKNKSAFNIFLLLGSLLTMAGGLKNVGFLNFISNAAKNF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LEHANLDSLIAVLFIIALFYLLHYFFASITAHVSALFALFVGIGSHIQGVNLQELSLFLM
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
FSLGIMGILTPYGTGPSTIYYGSGYISSKDFWKWGFIFGMVYLIVFLSVCVPWVKLIAYR
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]