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The map label for this gene is topA [H]

Identifier: 188526920

GI number: 188526920

Start: 111477

End: 113687

Strand: Direct

Name: topA [H]

Synonym: HPSH_00585

Alternate gene names: 188526920

Gene position: 111477-113687 (Clockwise)

Preceding gene: 188526917

Following gene: 188526921

Centisome position: 6.93

GC content: 40.39

Gene sequence:

>2211_bases
ATGAAGCACCTTATTATTGTAGAATCCCCCGCAAAAGCCAAAACCATTAAAAATTTTTTGGATAAAAATTACGAAGTCAT
CGCCTCTAAAGGGCATGTTAGGGATTTGTCCAAATTCGCTTTAGGCATTAAGATTGATGAAACAGGCTTTACCCCTAATT
ATGTCGTGGATAAAGACCATAAAGAGCTTGTCAAACAGATCATAGAGCTTTCTAAAAAGGCATCTACCACCTACATCGCT
ACCGATGAAGACAGAGAAGGGGAAGCGATAGGCTATCATGTGGCATGTTTGATTGGGGGGAAATTAGAGAGCTATCCTAG
GATTGTTTTTCATGAGATCACGCAAAATGCGATTTTAAACGCTCTAAAAACCCCACGACAAATTGACATGTCAAAAGTCA
ATGCCCAACAAGCCAGACGCTTTTTGGATCGGATCGTGGGTTTTAAGCTCAGTTCGTTGATTGCATCAAAAATCACTAAA
GGTTTGAGCGCTGGGCGGGTGCAAAGCGCGGCTTTAAAGCTTGTGATTGATAAAGAGAGGGAGATTGAAGCCTTTAAGCC
TTTAACCTACTTCACGCTAGACGCTTATTTTGAGCCGCATTTAGAAGCGCAACTCATTAGCTATAAAGGTAACAAACTCA
AAGCCCAAGAGCTTATTGATGGAAAAAAAGCTCAAGAGATTAAAAACGAATTAGAAAAAGAAAGCTACACTATTTCCAGT
ATTATTAAAAAATCTAAAAAATCCCCCACACCGCCCCCTTTCATGACTTCTACTTTGCAGCAAAGCGCTTCCAGTCTTTT
AGGCTTTTCGCCCATAAAAACCATGAGTATCGCTCAAAAATTGTATGAAGGCGTAGCCACCCCACAAGGGGTTATGGGGG
TGATCACTTACATGAGGACCGATAGCTTGAACATCGCTAAAGAAGCTTTAGAAGAAGCGAGGAATAAGATTTTAAAAGAC
TATGGCAAAGACTACTTACCCCCTAAAGCCAAAGTCTATTCCAGCAAGAATAAAAACGCCCAAGAAGCCCATGAAGCGAT
CAGGCCCACTTCTATTATTTTAGAGCCAAACGCTTTAAAAGACTACCTTAAGCCTGAAGAATTAAAGCTCTATACCTTAA
TTTACAAACGCTTCTTAGCTTCTCAAATGCAAGACGCTCTTTTTGAAAGCCAAAGCGTGGTTGTGGCTTGCGAAAAAGGC
GAGTTTAAAGCCAGTGGCAGAAAACTCCTTTTTGATGGCTATTATAAAATTTTAGGCAATGACGATAAGGACAAATTGCT
CCCCAATTTGAAAGAAAACGATCCTATTAAATTAGAAAAATTAGAGAGTAACGTGCATGTTACAGAACCTCCAGCGCGCT
ATTCAGAAGCGAGCTTGATTAAAGTTTTAGAAAGTTTAGGCATAGGCAGGCCCAGCACCTACGCCCCAACGATTTCCCTC
TTACAACACAGAGACTACATCAAGGTAGAAAAAAAGCAAATCAGCGCTTTAGAGAGCGCTTTTAAGGTGATAGAAATTTT
AGAAAAGCATTTTGAAGAAATCGTGGATTCCAAATTCAGCGCTTCTTTAGAAGAGGAACTGGACAATATCGCTCAAAATA
AAGCCGACTATCAGCAAGTCTTAAAGGACTTTTACTACCCCTTTATGGATAAAATTGAAGCTGGGAAAAAGAATATCATC
TCTCAAAAAGTGCATGAAAAAACCGGTCAATCATGCCCTAAATGCGGTGGGGAATTGGTCAAAAAAAATAGCCGTTATGG
GGAATTTGTCGCTTGCAACAATTACCCTAGATGCAAATATGTCAAACAAACTGAAAACACCAATGATGGAGCCAATCAGG
AATTGTGCGAAAAATGCGGAGGGGAAATGGTGCAAAAATTCAGCAGAAACGGGGCGTTTTTAGCTTGCAACAACTACCCT
GAATGCAAAAACACCAAATCGTTAAAAAACACCCCTAACGCAAACGAAATAATAGAAGGCGTGAAATGCCCAGAATGCGG
GGGGGATATTGCCTTAAAAAGGGGCAGGAAAGGCTCGTTTTATGGCTGCAGCAATTACCCTAAATGCAATTTTTTATCCA
ACCATAAGCCCATCAATAAGCGTTGTGAAAAATGCCATTATTTGATGAGTGAAAGAACCTATCGCAAAAAAAAGGCGCAT
GAATGCATCCAATGCAAGGAGCGCGTGTTTTTAGAGGAAGATGATGGCTAA

Upstream 100 bases:

>100_bases
TAGCGCTGTTATGGTTTTTATGGTTTAATAGGGCGTTTAGTCAAAATTCTATGCTACAATCATTCATTAATAGGTATAAA
CATTTCATTAAAGGCGTTCC

Downstream 100 bases:

>100_bases
AGAAAATCTGCCTATCGTTTTTGGGCCTGTTTTATCCAGGCGTTTTGGGAAGTCTTTGGGCGTGGATCTATCGCCCTCTA
AAAAGCAATGCAATTACAAT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MKHLIIVESPAKAKTIKNFLDKNYEVIASKGHVRDLSKFALGIKIDETGFTPNYVVDKDHKELVKQIIELSKKASTTYIA
TDEDREGEAIGYHVACLIGGKLESYPRIVFHEITQNAILNALKTPRQIDMSKVNAQQARRFLDRIVGFKLSSLIASKITK
GLSAGRVQSAALKLVIDKEREIEAFKPLTYFTLDAYFEPHLEAQLISYKGNKLKAQELIDGKKAQEIKNELEKESYTISS
IIKKSKKSPTPPPFMTSTLQQSASSLLGFSPIKTMSIAQKLYEGVATPQGVMGVITYMRTDSLNIAKEALEEARNKILKD
YGKDYLPPKAKVYSSKNKNAQEAHEAIRPTSIILEPNALKDYLKPEELKLYTLIYKRFLASQMQDALFESQSVVVACEKG
EFKASGRKLLFDGYYKILGNDDKDKLLPNLKENDPIKLEKLESNVHVTEPPARYSEASLIKVLESLGIGRPSTYAPTISL
LQHRDYIKVEKKQISALESAFKVIEILEKHFEEIVDSKFSASLEEELDNIAQNKADYQQVLKDFYYPFMDKIEAGKKNII
SQKVHEKTGQSCPKCGGELVKKNSRYGEFVACNNYPRCKYVKQTENTNDGANQELCEKCGGEMVQKFSRNGAFLACNNYP
ECKNTKSLKNTPNANEIIEGVKCPECGGDIALKRGRKGSFYGCSNYPKCNFLSNHKPINKRCEKCHYLMSERTYRKKKAH
ECIQCKERVFLEEDDG

Sequences:

>Translated_736_residues
MKHLIIVESPAKAKTIKNFLDKNYEVIASKGHVRDLSKFALGIKIDETGFTPNYVVDKDHKELVKQIIELSKKASTTYIA
TDEDREGEAIGYHVACLIGGKLESYPRIVFHEITQNAILNALKTPRQIDMSKVNAQQARRFLDRIVGFKLSSLIASKITK
GLSAGRVQSAALKLVIDKEREIEAFKPLTYFTLDAYFEPHLEAQLISYKGNKLKAQELIDGKKAQEIKNELEKESYTISS
IIKKSKKSPTPPPFMTSTLQQSASSLLGFSPIKTMSIAQKLYEGVATPQGVMGVITYMRTDSLNIAKEALEEARNKILKD
YGKDYLPPKAKVYSSKNKNAQEAHEAIRPTSIILEPNALKDYLKPEELKLYTLIYKRFLASQMQDALFESQSVVVACEKG
EFKASGRKLLFDGYYKILGNDDKDKLLPNLKENDPIKLEKLESNVHVTEPPARYSEASLIKVLESLGIGRPSTYAPTISL
LQHRDYIKVEKKQISALESAFKVIEILEKHFEEIVDSKFSASLEEELDNIAQNKADYQQVLKDFYYPFMDKIEAGKKNII
SQKVHEKTGQSCPKCGGELVKKNSRYGEFVACNNYPRCKYVKQTENTNDGANQELCEKCGGEMVQKFSRNGAFLACNNYP
ECKNTKSLKNTPNANEIIEGVKCPECGGDIALKRGRKGSFYGCSNYPKCNFLSNHKPINKRCEKCHYLMSERTYRKKKAH
ECIQCKERVFLEEDDG
>Mature_736_residues
MKHLIIVESPAKAKTIKNFLDKNYEVIASKGHVRDLSKFALGIKIDETGFTPNYVVDKDHKELVKQIIELSKKASTTYIA
TDEDREGEAIGYHVACLIGGKLESYPRIVFHEITQNAILNALKTPRQIDMSKVNAQQARRFLDRIVGFKLSSLIASKITK
GLSAGRVQSAALKLVIDKEREIEAFKPLTYFTLDAYFEPHLEAQLISYKGNKLKAQELIDGKKAQEIKNELEKESYTISS
IIKKSKKSPTPPPFMTSTLQQSASSLLGFSPIKTMSIAQKLYEGVATPQGVMGVITYMRTDSLNIAKEALEEARNKILKD
YGKDYLPPKAKVYSSKNKNAQEAHEAIRPTSIILEPNALKDYLKPEELKLYTLIYKRFLASQMQDALFESQSVVVACEKG
EFKASGRKLLFDGYYKILGNDDKDKLLPNLKENDPIKLEKLESNVHVTEPPARYSEASLIKVLESLGIGRPSTYAPTISL
LQHRDYIKVEKKQISALESAFKVIEILEKHFEEIVDSKFSASLEEELDNIAQNKADYQQVLKDFYYPFMDKIEAGKKNII
SQKVHEKTGQSCPKCGGELVKKNSRYGEFVACNNYPRCKYVKQTENTNDGANQELCEKCGGEMVQKFSRNGAFLACNNYP
ECKNTKSLKNTPNANEIIEGVKCPECGGDIALKRGRKGSFYGCSNYPKCNFLSNHKPINKRCEKCHYLMSERTYRKKKAH
ECIQCKERVFLEEDDG

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=692, Percent_Identity=22.5433526011561, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI10835218, Length=595, Percent_Identity=24.2016806722689, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI1787529, Length=751, Percent_Identity=37.9494007989348, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI48994931, Length=176, Percent_Identity=36.9318181818182, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788061, Length=567, Percent_Identity=23.6331569664903, Blast_Score=109, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI32563869, Length=624, Percent_Identity=23.7179487179487, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17555378, Length=463, Percent_Identity=26.133909287257, Blast_Score=118, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=571, Percent_Identity=23.1173380035026, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24585251, Length=633, Percent_Identity=25.5924170616114, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640096, Length=681, Percent_Identity=21.5859030837004, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 83158; Mature: 83158

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLIIVESPAKAKTIKNFLDKNYEVIASKGHVRDLSKFALGIKIDETGFTPNYVVDKDH
CCCEEEEECCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHEEEEEECCCCCCCCEECCHH
KELVKQIIELSKKASTTYIATDEDREGEAIGYHVACLIGGKLESYPRIVFHEITQNAILN
HHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEEEECCCHHHCCHHHHHHHHHHHHHH
ALKTPRQIDMSKVNAQQARRFLDRIVGFKLSSLIASKITKGLSAGRVQSAALKLVIDKER
HHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHHEEEECCCH
EIEAFKPLTYFTLDAYFEPHLEAQLISYKGNKLKAQELIDGKKAQEIKNELEKESYTISS
HHHHHCCCCEEEEHHHHCCCCHHHHEECCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHH
IIKKSKKSPTPPPFMTSTLQQSASSLLGFSPIKTMSIAQKLYEGVATPQGVMGVITYMRT
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
DSLNIAKEALEEARNKILKDYGKDYLPPKAKVYSSKNKNAQEAHEAIRPTSIILEPNALK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHCCCCEEEECCHHHH
DYLKPEELKLYTLIYKRFLASQMQDALFESQSVVVACEKGEFKASGRKLLFDGYYKILGN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECHHHHCCC
DDKDKLLPNLKENDPIKLEKLESNVHVTEPPARYSEASLIKVLESLGIGRPSTYAPTISL
CCHHHHCCCCCCCCCEEHEECCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
LQHRDYIKVEKKQISALESAFKVIEILEKHFEEIVDSKFSASLEEELDNIAQNKADYQQV
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LKDFYYPFMDKIEAGKKNIISQKVHEKTGQSCPKCGGELVKKNSRYGEFVACNNYPRCKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCE
VKQTENTNDGANQELCEKCGGEMVQKFSRNGAFLACNNYPECKNTKSLKNTPNANEIIEG
ECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCC
VKCPECGGDIALKRGRKGSFYGCSNYPKCNFLSNHKPINKRCEKCHYLMSERTYRKKKAH
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ECIQCKERVFLEEDDG
HHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKHLIIVESPAKAKTIKNFLDKNYEVIASKGHVRDLSKFALGIKIDETGFTPNYVVDKDH
CCCEEEEECCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHEEEEEECCCCCCCCEECCHH
KELVKQIIELSKKASTTYIATDEDREGEAIGYHVACLIGGKLESYPRIVFHEITQNAILN
HHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEEEECCCHHHCCHHHHHHHHHHHHHH
ALKTPRQIDMSKVNAQQARRFLDRIVGFKLSSLIASKITKGLSAGRVQSAALKLVIDKER
HHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHHEEEECCCH
EIEAFKPLTYFTLDAYFEPHLEAQLISYKGNKLKAQELIDGKKAQEIKNELEKESYTISS
HHHHHCCCCEEEEHHHHCCCCHHHHEECCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHH
IIKKSKKSPTPPPFMTSTLQQSASSLLGFSPIKTMSIAQKLYEGVATPQGVMGVITYMRT
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
DSLNIAKEALEEARNKILKDYGKDYLPPKAKVYSSKNKNAQEAHEAIRPTSIILEPNALK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHCCCCEEEECCHHHH
DYLKPEELKLYTLIYKRFLASQMQDALFESQSVVVACEKGEFKASGRKLLFDGYYKILGN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECHHHHCCC
DDKDKLLPNLKENDPIKLEKLESNVHVTEPPARYSEASLIKVLESLGIGRPSTYAPTISL
CCHHHHCCCCCCCCCEEHEECCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
LQHRDYIKVEKKQISALESAFKVIEILEKHFEEIVDSKFSASLEEELDNIAQNKADYQQV
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LKDFYYPFMDKIEAGKKNIISQKVHEKTGQSCPKCGGELVKKNSRYGEFVACNNYPRCKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCE
VKQTENTNDGANQELCEKCGGEMVQKFSRNGAFLACNNYPECKNTKSLKNTPNANEIIEG
ECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCC
VKCPECGGDIALKRGRKGSFYGCSNYPKCNFLSNHKPINKRCEKCHYLMSERTYRKKKAH
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ECIQCKERVFLEEDDG
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]