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The map label for this gene is prfA

Identifier: 188526883

GI number: 188526883

Start: 68395

End: 69453

Strand: Direct

Name: prfA

Synonym: HPSH_00375

Alternate gene names: 188526883

Gene position: 68395-69453 (Clockwise)

Preceding gene: 188526882

Following gene: 188526884

Centisome position: 4.25

GC content: 42.49

Gene sequence:

>1059_bases
ATGTCTATTCTAGCTGAAAAGCTTTCTTCCATTCTCAAGCGATACGACGAACTCACAGCGTTGCTTTCTAGTGCTGAAGT
GATTAGCGATATTAAAAAACTCACCGAATTGAGCAAAGAGCAAAGCTCCATTGAAGAAATCTCTGTAGCGAGTAAAGAAT
ATTTGAGCGTTTTAGAGAATATCAAAGAAAATAAAGAGCTTTTAGAAGACAAGGAATTGAGCGAACTGGCTAAAGAAGAG
TTAAAAATTTTAGAAATCAAAAAAAGCGATCTAGAAACCGCCATTAAGCAACTCCTTATCCCCAAAGATCCTAACGACGA
TAAAAACATTTATTTAGAGTTAAGGGCTGGCACGGGGGGCGATGAAGCGGGCATTTTTGTAGGGGATTTGTTTAAGGCGT
ATTGCCGTTATGCGGATTTGAAAAAATGGAAAGTAGAGATAGTGAGCTCTAGCGAAAACAGCGTAGGGGGCTATAAAGAA
ATCATCGCTTTGATTAAGGGTAAGGGCGTGTATTCAAGGCTCAAATTTGAAGCAGGCACGCATCGAGTCCAAAGAGTCCC
TGAAACAGAATCCCAAGGGCGCATCCACACTTCCGCTATCACAGTAGCGATCATGCCTGAAGTGGATGATGTGGAAGTTT
CTATCAACTCTAGCGATTTAAAGATTGAAGTGTTTCGCGCTGGCGGGCATGGGGGGCAATGCGTCAATACCACAGACTCT
GCGGTGCGCATCACGCACCTTCCCACTAATATCAGCGTGAGCATGCAAGATGAAAAATCCCAGCATAAAAACAAGGATAA
AGCCCTAAAAATCCTAAAAGCGCGCCTTTATGAAAAACAAATTGAAGAACAACAACTCGCTAACGCTAAAGACCGAAAGG
AGCAAGTGGGTAGTGGGGACAGGAGCGAAAGGATCCGCACTTATAATTACCCGCAAAACCGCTTGAGCGAACACCGAATC
AATTTAACTCTGTATAGTTTAGAAGAAATCATGCTTTCAGGGAATTTAGATGAAGTGATTAACCCTTTAATCGCCCACGC
CCAAAGCCAGTTTGAATAA

Upstream 100 bases:

>100_bases
AACTTCTTTAAGCTCAATGATGGATTTTATTATTGGGCTTCTTTTTGCGTTTTTTAGATTGTTGCATCTTTTATCCACAT
CTTTTTATAGGTAGTCTTGC

Downstream 100 bases:

>100_bases
GATTTTTACATGATACGCCCAAAAGCGATAAAGCACCCAAGCCTTTTTTAAACCACCCACTACTGCCAAAAACCCTAGCA
TGCTTTTCCAAATTTTCGCC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSILAEKLSSILKRYDELTALLSSAEVISDIKKLTELSKEQSSIEEISVASKEYLSVLENIKENKELLEDKELSELAKEE
LKILEIKKSDLETAIKQLLIPKDPNDDKNIYLELRAGTGGDEAGIFVGDLFKAYCRYADLKKWKVEIVSSSENSVGGYKE
IIALIKGKGVYSRLKFEAGTHRVQRVPETESQGRIHTSAITVAIMPEVDDVEVSINSSDLKIEVFRAGGHGGQCVNTTDS
AVRITHLPTNISVSMQDEKSQHKNKDKALKILKARLYEKQIEEQQLANAKDRKEQVGSGDRSERIRTYNYPQNRLSEHRI
NLTLYSLEEIMLSGNLDEVINPLIAHAQSQFE

Sequences:

>Translated_352_residues
MSILAEKLSSILKRYDELTALLSSAEVISDIKKLTELSKEQSSIEEISVASKEYLSVLENIKENKELLEDKELSELAKEE
LKILEIKKSDLETAIKQLLIPKDPNDDKNIYLELRAGTGGDEAGIFVGDLFKAYCRYADLKKWKVEIVSSSENSVGGYKE
IIALIKGKGVYSRLKFEAGTHRVQRVPETESQGRIHTSAITVAIMPEVDDVEVSINSSDLKIEVFRAGGHGGQCVNTTDS
AVRITHLPTNISVSMQDEKSQHKNKDKALKILKARLYEKQIEEQQLANAKDRKEQVGSGDRSERIRTYNYPQNRLSEHRI
NLTLYSLEEIMLSGNLDEVINPLIAHAQSQFE
>Mature_351_residues
SILAEKLSSILKRYDELTALLSSAEVISDIKKLTELSKEQSSIEEISVASKEYLSVLENIKENKELLEDKELSELAKEEL
KILEIKKSDLETAIKQLLIPKDPNDDKNIYLELRAGTGGDEAGIFVGDLFKAYCRYADLKKWKVEIVSSSENSVGGYKEI
IALIKGKGVYSRLKFEAGTHRVQRVPETESQGRIHTSAITVAIMPEVDDVEVSINSSDLKIEVFRAGGHGGQCVNTTDSA
VRITHLPTNISVSMQDEKSQHKNKDKALKILKARLYEKQIEEQQLANAKDRKEQVGSGDRSERIRTYNYPQNRLSEHRIN
LTLYSLEEIMLSGNLDEVINPLIAHAQSQFE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=324, Percent_Identity=42.5925925925926, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI34577120, Length=320, Percent_Identity=40.3125, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI166795305, Length=225, Percent_Identity=40.8888888888889, Blast_Score=178, Evalue=8e-45,
Organism=Escherichia coli, GI1787462, Length=349, Percent_Identity=53.8681948424069, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI2367172, Length=337, Percent_Identity=36.2017804154303, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=286, Percent_Identity=36.3636363636364, Blast_Score=181, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=308, Percent_Identity=43.8311688311688, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI19921226, Length=340, Percent_Identity=36.1764705882353, Blast_Score=211, Evalue=5e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39577; Mature: 39446

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILAEKLSSILKRYDELTALLSSAEVISDIKKLTELSKEQSSIEEISVASKEYLSVLEN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKENKELLEDKELSELAKEELKILEIKKSDLETAIKQLLIPKDPNDDKNIYLELRAGTGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCC
DEAGIFVGDLFKAYCRYADLKKWKVEIVSSSENSVGGYKEIIALIKGKGVYSRLKFEAGT
CCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
HRVQRVPETESQGRIHTSAITVAIMPEVDDVEVSINSSDLKIEVFRAGGHGGQCVNTTDS
HHHHCCCCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCEEEEEEEECCCCCCEECCCCC
AVRITHLPTNISVSMQDEKSQHKNKDKALKILKARLYEKQIEEQQLANAKDRKEQVGSGD
EEEEEECCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCC
RSERIRTYNYPQNRLSEHRINLTLYSLEEIMLSGNLDEVINPLIAHAQSQFE
CCCCCEECCCCHHHHHHHEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SILAEKLSSILKRYDELTALLSSAEVISDIKKLTELSKEQSSIEEISVASKEYLSVLEN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKENKELLEDKELSELAKEELKILEIKKSDLETAIKQLLIPKDPNDDKNIYLELRAGTGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCC
DEAGIFVGDLFKAYCRYADLKKWKVEIVSSSENSVGGYKEIIALIKGKGVYSRLKFEAGT
CCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
HRVQRVPETESQGRIHTSAITVAIMPEVDDVEVSINSSDLKIEVFRAGGHGGQCVNTTDS
HHHHCCCCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCEEEEEEEECCCCCCEECCCCC
AVRITHLPTNISVSMQDEKSQHKNKDKALKILKARLYEKQIEEQQLANAKDRKEQVGSGD
EEEEEECCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCC
RSERIRTYNYPQNRLSEHRINLTLYSLEEIMLSGNLDEVINPLIAHAQSQFE
CCCCCEECCCCHHHHHHHEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA