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The map label for this gene is ddeIM [H]

Identifier: 188526860

GI number: 188526860

Start: 47321

End: 48388

Strand: Reverse

Name: ddeIM [H]

Synonym: HPSH_00250

Alternate gene names: 188526860

Gene position: 48388-47321 (Counterclockwise)

Preceding gene: 188526861

Following gene: 188526859

Centisome position: 3.01

GC content: 38.76

Gene sequence:

>1068_bases
ATGAATTATAAAATTTTAGATTTATTTTGTGGGGCTGGGGGTTTTAGCGCTGGGTTGGAGTGTTTAAAAGAGTTTGACGC
TTTAATAGGGCTAGATTGCGATAAACAAGCCCTAATCACTTTTGAAAACAACCATAAAAACGCTATGGGTATTTGTGGGG
ACATCACTCAAGCTGAAATTAAAGAAAAAGTCATCAAACTAGCCCAAACATTAGAAATCAACATGATCATTGGCGGGCCT
CCATGTCAAGGCTTTTCTAATAAAGGGAAAAATCTAGGGCTAAAAGACCCTAGGAATTTTTTATTCTTAGAATATATAGA
GATAGTGAAAGCCATAAAACCAGAAATTTTTATCATTGAAAATGTGAAAAACCTCATCTCTTGCGCTAAAGGCTATTTTT
TGGAAGAAATTAAAGAAAAATTGAACGCTTTAGGGTATCAATTAAGCTATCAAATCCTAAACGCTAAAGATTATGGCGTG
CCTCAAAACAGAGAGAGGGCTTTTATTGTGGGGGCTAGTCGTTTTAGTTTTGATTTTAATCTTTTAGAACCTTCTCAAAG
CGTGAACGTTCAAGATGCGATAAGCGATTTAGCCTATCTTTGTTCTAATGAGGGGGCGTTTGAGAGCGATTATTTAAACC
CTATCCAATCAAGCTATCAAGCCTTGATGCGAAAAGATAGCCCCAAACTCTACAACCATCAAGCCACCAACCACTCGCAA
GCCGCTTTAGAGAAATTAAAGCTCATTAACAAAGAACAGGGTAAAGAATGCTTGCCTAAAAACTTGCATGGCAAACAGCA
ATTCAAAAGCACATGGGGGCGTTTGAATTGGAATAAAGTAAGCCCCACCATAGACACACGATTTGACACTCCAAGCAACG
GCACCAATTCCCACCCTGAATTGCACCGCTCTATCACGCCCAGAGAAGCCGCCAGGATACAAAGTTTTAGCGATAATTAT
ATCTTTTATGGCAATAAAACGAGTGTTTGTAAGCAAATCGGTAACGCTGTACCTCCTCTTCTAGCCCTAGCTTTAGGCAA
AGCGATCTTAAAAAGCTTAAGAAAATGA

Upstream 100 bases:

>100_bases
ATATTCTAAATCGCAATAAAGACAACTTAGAGTTTGTGCAATTGCGTTTTGAAACCGATGATTTTCCAACGCTTATTAAT
CGTATTTATGAAAGCTTAAA

Downstream 100 bases:

>100_bases
TACAAATTCATCACGCTAACGCTTTTGAAATCATCAAAGACTTTTACCAGCAAGATTTAAAAGTGGATGCAATCATCACG
GACCCTCCTTATAACATTTC

Product: cytosine specific DNA methyltransferase (DDEM)

Products: NA

Alternate protein names: M.DdeI; Cytosine-specific methyltransferase DdeI [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MNYKILDLFCGAGGFSAGLECLKEFDALIGLDCDKQALITFENNHKNAMGICGDITQAEIKEKVIKLAQTLEINMIIGGP
PCQGFSNKGKNLGLKDPRNFLFLEYIEIVKAIKPEIFIIENVKNLISCAKGYFLEEIKEKLNALGYQLSYQILNAKDYGV
PQNRERAFIVGASRFSFDFNLLEPSQSVNVQDAISDLAYLCSNEGAFESDYLNPIQSSYQALMRKDSPKLYNHQATNHSQ
AALEKLKLINKEQGKECLPKNLHGKQQFKSTWGRLNWNKVSPTIDTRFDTPSNGTNSHPELHRSITPREAARIQSFSDNY
IFYGNKTSVCKQIGNAVPPLLALALGKAILKSLRK

Sequences:

>Translated_355_residues
MNYKILDLFCGAGGFSAGLECLKEFDALIGLDCDKQALITFENNHKNAMGICGDITQAEIKEKVIKLAQTLEINMIIGGP
PCQGFSNKGKNLGLKDPRNFLFLEYIEIVKAIKPEIFIIENVKNLISCAKGYFLEEIKEKLNALGYQLSYQILNAKDYGV
PQNRERAFIVGASRFSFDFNLLEPSQSVNVQDAISDLAYLCSNEGAFESDYLNPIQSSYQALMRKDSPKLYNHQATNHSQ
AALEKLKLINKEQGKECLPKNLHGKQQFKSTWGRLNWNKVSPTIDTRFDTPSNGTNSHPELHRSITPREAARIQSFSDNY
IFYGNKTSVCKQIGNAVPPLLALALGKAILKSLRK
>Mature_355_residues
MNYKILDLFCGAGGFSAGLECLKEFDALIGLDCDKQALITFENNHKNAMGICGDITQAEIKEKVIKLAQTLEINMIIGGP
PCQGFSNKGKNLGLKDPRNFLFLEYIEIVKAIKPEIFIIENVKNLISCAKGYFLEEIKEKLNALGYQLSYQILNAKDYGV
PQNRERAFIVGASRFSFDFNLLEPSQSVNVQDAISDLAYLCSNEGAFESDYLNPIQSSYQALMRKDSPKLYNHQATNHSQ
AALEKLKLINKEQGKECLPKNLHGKQQFKSTWGRLNWNKVSPTIDTRFDTPSNGTNSHPELHRSITPREAARIQSFSDNY
IFYGNKTSVCKQIGNAVPPLLALALGKAILKSLRK

Specific function: This methylase recognizes the double-stranded sequence CTNAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the DdeI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI195927037, Length=453, Percent_Identity=24.7240618101545, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI4503351, Length=453, Percent_Identity=24.7240618101545, Blast_Score=129, Evalue=6e-30,
Organism=Escherichia coli, GI1788271, Length=389, Percent_Identity=24.6786632390745, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 39724; Mature: 39724

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKILDLFCGAGGFSAGLECLKEFDALIGLDCDKQALITFENNHKNAMGICGDITQAEI
CCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHH
KEKVIKLAQTLEINMIIGGPPCQGFSNKGKNLGLKDPRNFLFLEYIEIVKAIKPEIFIIE
HHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHH
NVKNLISCAKGYFLEEIKEKLNALGYQLSYQILNAKDYGVPQNRERAFIVGASRFSFDFN
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEECCEEEEEEE
LLEPSQSVNVQDAISDLAYLCSNEGAFESDYLNPIQSSYQALMRKDSPKLYNHQATNHSQ
EECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHH
AALEKLKLINKEQGKECLPKNLHGKQQFKSTWGRLNWNKVSPTIDTRFDTPSNGTNSHPE
HHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCH
LHRSITPREAARIQSFSDNYIFYGNKTSVCKQIGNAVPPLLALALGKAILKSLRK
HHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYKILDLFCGAGGFSAGLECLKEFDALIGLDCDKQALITFENNHKNAMGICGDITQAEI
CCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHH
KEKVIKLAQTLEINMIIGGPPCQGFSNKGKNLGLKDPRNFLFLEYIEIVKAIKPEIFIIE
HHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHH
NVKNLISCAKGYFLEEIKEKLNALGYQLSYQILNAKDYGVPQNRERAFIVGASRFSFDFN
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEECCEEEEEEE
LLEPSQSVNVQDAISDLAYLCSNEGAFESDYLNPIQSSYQALMRKDSPKLYNHQATNHSQ
EECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHH
AALEKLKLINKEQGKECLPKNLHGKQQFKSTWGRLNWNKVSPTIDTRFDTPSNGTNSHPE
HHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCH
LHRSITPREAARIQSFSDNYIFYGNKTSVCKQIGNAVPPLLALALGKAILKSLRK
HHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2823226 [H]