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The map label for this gene is mboIBM [H]

Identifier: 188526859

GI number: 188526859

Start: 46626

End: 47324

Strand: Reverse

Name: mboIBM [H]

Synonym: HPSH_00245

Alternate gene names: 188526859

Gene position: 47324-46626 (Counterclockwise)

Preceding gene: 188526860

Following gene: 188526858

Centisome position: 2.94

GC content: 39.48

Gene sequence:

>699_bases
ATGATACAAATTCATCACGCTAACGCTTTTGAAATCATCAAAGACTTTTACCAGCAAGATTTAAAAGTGGATGCAATCAT
CACGGACCCTCCTTATAACATTTCGGTTAAAAACAATTTTTCCACCCTAAAGAGCGCTACAAGGCAAGGCATAGATTTTG
GGGAATGGGATAAAAATTTCAGGCTTTTAGAATGGATCAAGCGCTACGCCCCCTTAGTCAATCCAAACGGCTGCATGGTT
ATTTTTTGCTCTTACAGGTTTATAAGCTATATCGCTGATTTTTTAGAAGAAAACGGCTTTGTGGTCAAAGACTTTATCCA
ATGGGTTAAAAATAATCCCATGCCAAGAAACATTAACCGGCGCTATGTCCAGGACACGGAATTTGCCCTGTGGGCGGTTA
AAAAGAAAGCTAAATGGGTGTTTAACAAGCCAAAAAATGAAAAATATTTACGGCCCTTGATTTTAAAAAGCCCTGTGGTT
GGCGGGCTTGAAAGAGTTAAACACCCCACGCAAAAAAGCCTAGCCTTAATGGAAAAAATCATTTCCATCCACACAAACCC
TAATGACATCGTGCTAGATCCTTTCATGGGGAGCGGCACCACCGGCTTAGCATGCAAGCACTTGAAACGAAATTTTATCG
GTATAGAATCAGAAAAAGAATATTTTCAAATCGCCAAAAAGCGTTTGAATCTGTTTTAA

Upstream 100 bases:

>100_bases
TTTATGGCAATAAAACGAGTGTTTGTAAGCAAATCGGTAACGCTGTACCTCCTCTTCTAGCCCTAGCTTTAGGCAAAGCG
ATCTTAAAAAGCTTAAGAAA

Downstream 100 bases:

>100_bases
AAACGCTATTTGAATGAGATTGTGTTATAGTTATTAAGTTATTAAAAGGATATTTTGAATGAAAAGAATGTTAGCGGAGT
TTGAAAAAATCCAAGCGATT

Product: adenine specific DNA methyltransferase (dpnA)

Products: NA

Alternate protein names: M.MboIB; Adenine-specific methyltransferase MboIB; M.MboI C [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MIQIHHANAFEIIKDFYQQDLKVDAIITDPPYNISVKNNFSTLKSATRQGIDFGEWDKNFRLLEWIKRYAPLVNPNGCMV
IFCSYRFISYIADFLEENGFVVKDFIQWVKNNPMPRNINRRYVQDTEFALWAVKKKAKWVFNKPKNEKYLRPLILKSPVV
GGLERVKHPTQKSLALMEKIISIHTNPNDIVLDPFMGSGTTGLACKHLKRNFIGIESEKEYFQIAKKRLNLF

Sequences:

>Translated_232_residues
MIQIHHANAFEIIKDFYQQDLKVDAIITDPPYNISVKNNFSTLKSATRQGIDFGEWDKNFRLLEWIKRYAPLVNPNGCMV
IFCSYRFISYIADFLEENGFVVKDFIQWVKNNPMPRNINRRYVQDTEFALWAVKKKAKWVFNKPKNEKYLRPLILKSPVV
GGLERVKHPTQKSLALMEKIISIHTNPNDIVLDPFMGSGTTGLACKHLKRNFIGIESEKEYFQIAKKRLNLF
>Mature_232_residues
MIQIHHANAFEIIKDFYQQDLKVDAIITDPPYNISVKNNFSTLKSATRQGIDFGEWDKNFRLLEWIKRYAPLVNPNGCMV
IFCSYRFISYIADFLEENGFVVKDFIQWVKNNPMPRNINRRYVQDTEFALWAVKKKAKWVFNKPKNEKYLRPLILKSPVV
GGLERVKHPTQKSLALMEKIISIHTNPNDIVLDPFMGSGTTGLACKHLKRNFIGIESEKEYFQIAKKRLNLF

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=254, Percent_Identity=30.7086614173228, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 27132; Mature: 27132

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQIHHANAFEIIKDFYQQDLKVDAIITDPPYNISVKNNFSTLKSATRQGIDFGEWDKNF
CEEEECCCHHHHHHHHHHCCCEEEEEEECCCCEEEECCCHHHHHHHHHCCCCCCCCCCCC
RLLEWIKRYAPLVNPNGCMVIFCSYRFISYIADFLEENGFVVKDFIQWVKNNPMPRNINR
HHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCC
RYVQDTEFALWAVKKKAKWVFNKPKNEKYLRPLILKSPVVGGLERVKHPTQKSLALMEKI
HHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHH
ISIHTNPNDIVLDPFMGSGTTGLACKHLKRNFIGIESEKEYFQIAKKRLNLF
HHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIQIHHANAFEIIKDFYQQDLKVDAIITDPPYNISVKNNFSTLKSATRQGIDFGEWDKNF
CEEEECCCHHHHHHHHHHCCCEEEEEEECCCCEEEECCCHHHHHHHHHCCCCCCCCCCCC
RLLEWIKRYAPLVNPNGCMVIFCSYRFISYIADFLEENGFVVKDFIQWVKNNPMPRNINR
HHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCC
RYVQDTEFALWAVKKKAKWVFNKPKNEKYLRPLILKSPVVGGLERVKHPTQKSLALMEKI
HHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHH
ISIHTNPNDIVLDPFMGSGTTGLACKHLKRNFIGIESEKEYFQIAKKRLNLF
HHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8506128 [H]