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The map label for this gene is citA [H]

Identifier: 187930628

GI number: 187930628

Start: 3731251

End: 3732483

Strand: Reverse

Name: citA [H]

Synonym: Rpic_3564

Alternate gene names: 187930628

Gene position: 3732483-3731251 (Counterclockwise)

Preceding gene: 187930632

Following gene: 187930624

Centisome position: 94.67

GC content: 69.75

Gene sequence:

>1233_bases
ATGACCACCTACCTCAACGCCGCCGAAGCCGCCGAGCGCCTGCACGTCTCGCGCCCCACGCTGTACGCCTATGTAAGCCG
CGGGCTGCTGTCGGCGTATCCGTCGCCCGACGGACGCGGCAGCCGCTACCGCGAGGCCGACGTGGCTCGGCTGGCCGATC
AGCGTTCCGGCGGACGGCGCCCGCGTCAGGTGGTGCGCCATGCGCTGGACTGGGGTCTGCCCGTCATGGAATCGGCCATC
ACACTCATCGATCACGGGCGGTTGTTCTATCGGGGCGTGCCCGCCTTGGAACTGGCCGAACGCGCCACGCTGGAAACCGT
TGCGGCGCGGTTGTGGCAATGCGAGGAAGGGGTTGCCTTCAAGGTGCCCGCGCCGGTGTTGCCGAAGAGCTGGTACGCGG
CCCTTGCGTCGTTGGAAGACGCGGATATCCGCCAACGCTGCATGGCGCTATGCGCGCTTGCCTTGCCGGCGCTGGACGAG
GCCGCGTGGCGGCAGGACTCCGCACGTACGGCGCGCGATGCCGGTGCGCTGCTGCGCGTCGTGTTTGCCGCCATGCTCGG
CCGCAAGCCCGACACCGCACCCGTGCATCTGCAATGCCAGCAGGCATGGAACCTTGACGCGCACGCCGCCGAGGCCATCC
GCGTCGCATTGGTGCTGTGCGCCGATCACGAACTCAATGCCTCGAGCTTTGCGGCGCGCGTGGTTGCATCGACCGGCGCC
AGCCTGGGCGCCGTGGTCAGTGCGGGCTTGGCTGCGCTCTCCGGTGGCCGGCATGGCGGCACCACGGCGCGCGTGGAGGC
ATTGCTCAGCGAGCTGGAAGGGAAGCGCTCCGTGGCAACGGCTTTGCGTCGTCGGCTGGATCGCGGCGATACGCTGCCGG
GCTTTGGCCACCCGCTGTATCGCCATGCCGACCCGCGGGCGGAAGCGATCCTGGCACACTTGCCGAAGACGGGTGCGGTA
CGCAAGCGGCTGCAAACGGTGATGGATGCCGTGGCCGGATTGACCGGTGATGGGCCATCGGTGGATTTCGCGCTGGTGGC
CACCCGGCGTGTGCTCGGGCTGCCGGCAGGTTCAGGCTTTGGCCTGTTTGCCGCTGGGCGTACGGCAGGCTGGATTGCGC
ACGCGCTGGAGCAGCGCGCGGCGGGGCAGTTGATCCGCCCGAGAGCCGCCTATGTGGGGCCGATGCCGGCCGCGCTGGAG
GCACCGGCGGGCCGCATCATTCGTGCGCGCTGA

Upstream 100 bases:

>100_bases
GCGGCCATTACAGTCCCGCGCCGCCGATAGCGTCAACATTGACTAGACTGATCAACCTCGATTGCCGTCCACTTCATCCG
GCCCCGATGACCACCTCCCG

Downstream 100 bases:

>100_bases
GGCGCGATCAGGCCTGCGGAATCTCGATCTTCACCTCAAGCACTTCCAGGTTGTCCTGGCGCTCGAGGTTGACCTTGATG
TCGTCGTTGCCGATCTTCAC

Product: excisionase family DNA binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTTYLNAAEAAERLHVSRPTLYAYVSRGLLSAYPSPDGRGSRYREADVARLADQRSGGRRPRQVVRHALDWGLPVMESAI
TLIDHGRLFYRGVPALELAERATLETVAARLWQCEEGVAFKVPAPVLPKSWYAALASLEDADIRQRCMALCALALPALDE
AAWRQDSARTARDAGALLRVVFAAMLGRKPDTAPVHLQCQQAWNLDAHAAEAIRVALVLCADHELNASSFAARVVASTGA
SLGAVVSAGLAALSGGRHGGTTARVEALLSELEGKRSVATALRRRLDRGDTLPGFGHPLYRHADPRAEAILAHLPKTGAV
RKRLQTVMDAVAGLTGDGPSVDFALVATRRVLGLPAGSGFGLFAAGRTAGWIAHALEQRAAGQLIRPRAAYVGPMPAALE
APAGRIIRAR

Sequences:

>Translated_410_residues
MTTYLNAAEAAERLHVSRPTLYAYVSRGLLSAYPSPDGRGSRYREADVARLADQRSGGRRPRQVVRHALDWGLPVMESAI
TLIDHGRLFYRGVPALELAERATLETVAARLWQCEEGVAFKVPAPVLPKSWYAALASLEDADIRQRCMALCALALPALDE
AAWRQDSARTARDAGALLRVVFAAMLGRKPDTAPVHLQCQQAWNLDAHAAEAIRVALVLCADHELNASSFAARVVASTGA
SLGAVVSAGLAALSGGRHGGTTARVEALLSELEGKRSVATALRRRLDRGDTLPGFGHPLYRHADPRAEAILAHLPKTGAV
RKRLQTVMDAVAGLTGDGPSVDFALVATRRVLGLPAGSGFGLFAAGRTAGWIAHALEQRAAGQLIRPRAAYVGPMPAALE
APAGRIIRAR
>Mature_409_residues
TTYLNAAEAAERLHVSRPTLYAYVSRGLLSAYPSPDGRGSRYREADVARLADQRSGGRRPRQVVRHALDWGLPVMESAIT
LIDHGRLFYRGVPALELAERATLETVAARLWQCEEGVAFKVPAPVLPKSWYAALASLEDADIRQRCMALCALALPALDEA
AWRQDSARTARDAGALLRVVFAAMLGRKPDTAPVHLQCQQAWNLDAHAAEAIRVALVLCADHELNASSFAARVVASTGAS
LGAVVSAGLAALSGGRHGGTTARVEALLSELEGKRSVATALRRRLDRGDTLPGFGHPLYRHADPRAEAILAHLPKTGAVR
KRLQTVMDAVAGLTGDGPSVDFALVATRRVLGLPAGSGFGLFAAGRTAGWIAHALEQRAAGQLIRPRAAYVGPMPAALEA
PAGRIIRAR

Specific function: Catalyzes The Synthesis Of 2-Methylcitrate From Propionyl-CoA And Oxaloacetate. Also Catalyzes The Condensation Of Oxaloacetate With Acetyl-CoA But With A Lower Specificity. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=203, Percent_Identity=33.4975369458128, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786939, Length=192, Percent_Identity=34.8958333333333, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 43644; Mature: 43512

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYLNAAEAAERLHVSRPTLYAYVSRGLLSAYPSPDGRGSRYREADVARLADQRSGGRR
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
PRQVVRHALDWGLPVMESAITLIDHGRLFYRGVPALELAERATLETVAARLWQCEEGVAF
HHHHHHHHHHCCCHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
KVPAPVLPKSWYAALASLEDADIRQRCMALCALALPALDEAAWRQDSARTARDAGALLRV
ECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VFAAMLGRKPDTAPVHLQCQQAWNLDAHAAEAIRVALVLCADHELNASSFAARVVASTGA
HHHHHHCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
SLGAVVSAGLAALSGGRHGGTTARVEALLSELEGKRSVATALRRRLDRGDTLPGFGHPLY
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHH
RHADPRAEAILAHLPKTGAVRKRLQTVMDAVAGLTGDGPSVDFALVATRRVLGLPAGSGF
HCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCC
GLFAAGRTAGWIAHALEQRAAGQLIRPRAAYVGPMPAALEAPAGRIIRAR
CEEECCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHCCCCCCEEECC
>Mature Secondary Structure 
TTYLNAAEAAERLHVSRPTLYAYVSRGLLSAYPSPDGRGSRYREADVARLADQRSGGRR
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
PRQVVRHALDWGLPVMESAITLIDHGRLFYRGVPALELAERATLETVAARLWQCEEGVAF
HHHHHHHHHHCCCHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
KVPAPVLPKSWYAALASLEDADIRQRCMALCALALPALDEAAWRQDSARTARDAGALLRV
ECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VFAAMLGRKPDTAPVHLQCQQAWNLDAHAAEAIRVALVLCADHELNASSFAARVVASTGA
HHHHHHCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
SLGAVVSAGLAALSGGRHGGTTARVEALLSELEGKRSVATALRRRLDRGDTLPGFGHPLY
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHH
RHADPRAEAILAHLPKTGAVRKRLQTVMDAVAGLTGDGPSVDFALVATRRVLGLPAGSGF
HCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCC
GLFAAGRTAGWIAHALEQRAAGQLIRPRAAYVGPMPAALEAPAGRIIRAR
CEEECCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]