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The map label for this gene is pilQ [H]

Identifier: 187930316

GI number: 187930316

Start: 3398127

End: 3400292

Strand: Reverse

Name: pilQ [H]

Synonym: Rpic_3249

Alternate gene names: 187930316

Gene position: 3400292-3398127 (Counterclockwise)

Preceding gene: 187930317

Following gene: 187930315

Centisome position: 86.25

GC content: 64.04

Gene sequence:

>2166_bases
ATGACGATGCGTCTTGTGAAGGGCGGCCTGTCGCAGGCAACCCGAGTGGCCCGGGGGGGCGCGGTGGCATGGCTTTCGCT
GTGCCTGTTCATGGTGGCCGGTCAGGCGGTCGCTCAGCAGGCAGCGCCTGCGGCGGCCGTCTCCGGCAACACGATTGAAA
AGGTAGAGCAGGCCACGGCCGGTGAATCCACCGTGGTGACGGTCACGCTCAAGAGCGCGCCGACCCAGAAGCCGGTGGAG
TTCAGCACGCAGCAGCCTGCGCGTATCGCCATCGACTTCTTCGGGGCGACCTTCGCGCAAGGCCGCGCCAATTACCAGTA
CGGCGGCAAGCTGCTGCGTTCGGCGAACGTCGTGCAGATCGGCGACCGTACCCGCGTCGTGCTCGATCTATCGCGCCAGA
CGCAGTACAAGTCGGAGGTGCGTGGCAACCAGTACGTACTGACGCTGGAAGCTGCGCCAGTGGCGGCGGCTGTCGCGGCG
CCGACATTCTCGGCCCCGGCCCCCGTCGCGGGCGCCGAGCGCCCTGCGGTGCGCAATGTTGATTTCCGCCGCGGCGAGGA
TCTCGCCGGCCGCGTGGTGGTCGACCTGTCGACTGCCAACTCGGCGATCAACATCGCCCAGCAAGGCCAGAACCTCGTCG
TGGACTTCGTCGGTGCGACGCTGCCACAGTCGCTGCGCCGTCGCTATGACGTGAGCGATTTCGGCACGCCGGTGCAGGCC
ATGCGTGCCACCGACAACGGCACAGGCGCACGCCTGATCATCGAGCCGCGCGGTAACTGGCAATACAGCTCGTACCAGAC
CGACACGCAGTTCGTGGTGGAGGTGCGTCCGACCAAGGAAGACCCGAACAAGCTGATTTCCGGCCCCGGCTACCGCGGTG
AGCGCCTGTCGCTGAACTTCCAGAACATCGATGTGCGTTCTTTGCTGCAAGTCTTCGCCGACTTCACGAACCTCAACATC
GTGACCAGCGATAGCGTGACGGGCACCCTTTCGCTGCGCCTGAAAGATGTGCCCTGGGATCAGGCACTGCAGATCGTGCT
CGATTCGAAGGGGCTGGCATCGCGTCGCAACGGCAACGTCCTGTGGGTTGCCCCGCGCGGTGAGCTGGCCACGAAGGAAA
AGGCCGAGCTTGAGTCGCAGCAGCAAGTGACCGAGCTGGAGCCACTGCGCAGCCAGGTGTTCCGCCTGAACTACCAAAGC
GCTGGGGATGTGCGCAACATGCTGCTGGGTGCCGGCGCCGCGGGTGGGGCAGGCGGTGTCGCTGGCGGTGCCGCGACTTC
CCGGATCCTGTCCAAGCGCGGCTCGCTCACGGCGGATACGCGTACGAATCAACTGTTCGTCTCCGACATCCCGAGCAAGC
TTGAAGAGGTGCAGGCCTTCCTCTTGAAGATCGACATCCCGGTTCGCCAGGTGATGATCGAAGCGCGCATCGTCGAGGCG
GACGACACGTTCAGCCGCAACTTGGGTGCGAAGCTGGGCTTCGCGTCGAAGACGAATGGCGCTGGTTACGGCAATACCTA
CACCAACGTGGTGTCGCCGATTACGCAGGGCGCCACGTGGGACAACAGCCCGGCGATCAGCTTCCCCGCCAACGGCATCA
ATGGTGTGAACGCGGCCAGCGTGGCCGTGAGCCTGTTCAACGCCGGTGCCGGCCGCTTCCTGGCGCTCGAGTTGTCTGCG
CTGGAAGCTGATGGCCGCGGCAAGATCGTCTCCAGCCCGCGCGTGGTCACTGCCGACAACATCAAGGCGCTGATCGAGCA
AGGTACTGAGATTCCGTACCAGCAGGCGACGTCTTCCGGTGCGACTTCGGTGTCGTTCAAGAAGGCCAACCTGAAGCTGG
AAGTGACGCCGAAGATCACGCCGGACGGCAACATCTTCCTGGATGTCGACGTCAACAAGGACAGCGTGGGGATCCAGACC
ACCAACGGCTTCGCCATCGACACCAAGCACGTGCAGACGCAGGTGCTGGTCGAGAACGGCGGCACGGTCGTGATCGGCGG
TATCTACACGCAGAACGAGCGGACCGACATCAACAAGGTGCCGCTGCTAGGCGACATTCCGGTGCTCGGCAATCTGTTCA
AGAGCACGAGCAAGATCAACAACCGCACGGAGCTGCTGGTCTTCCTGACGCCGCGTGTGCTGTCCGATCAACTGTCGCTG
AAGTAA

Upstream 100 bases:

>100_bases
GCATCGGAGACCAGGAACTCACCCTGCGTGAGCTGGTGCGCGAAGGCACGACCGAATGGAAAGAAAAAATGACGACCCTG
AAAGTTCAGGAGAGCGCGCA

Downstream 100 bases:

>100_bases
TCGGCGGCGGTATCGCGATTGGAAAGCCCGGCCTCCTGAATGGAGCGCCGGGCTTTTTTGTGTCCTGCTGCCCGGTTTGT
GCAGGATGGTGACCAAATGC

Product: type IV pilus secretin PilQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MTMRLVKGGLSQATRVARGGAVAWLSLCLFMVAGQAVAQQAAPAAAVSGNTIEKVEQATAGESTVVTVTLKSAPTQKPVE
FSTQQPARIAIDFFGATFAQGRANYQYGGKLLRSANVVQIGDRTRVVLDLSRQTQYKSEVRGNQYVLTLEAAPVAAAVAA
PTFSAPAPVAGAERPAVRNVDFRRGEDLAGRVVVDLSTANSAINIAQQGQNLVVDFVGATLPQSLRRRYDVSDFGTPVQA
MRATDNGTGARLIIEPRGNWQYSSYQTDTQFVVEVRPTKEDPNKLISGPGYRGERLSLNFQNIDVRSLLQVFADFTNLNI
VTSDSVTGTLSLRLKDVPWDQALQIVLDSKGLASRRNGNVLWVAPRGELATKEKAELESQQQVTELEPLRSQVFRLNYQS
AGDVRNMLLGAGAAGGAGGVAGGAATSRILSKRGSLTADTRTNQLFVSDIPSKLEEVQAFLLKIDIPVRQVMIEARIVEA
DDTFSRNLGAKLGFASKTNGAGYGNTYTNVVSPITQGATWDNSPAISFPANGINGVNAASVAVSLFNAGAGRFLALELSA
LEADGRGKIVSSPRVVTADNIKALIEQGTEIPYQQATSSGATSVSFKKANLKLEVTPKITPDGNIFLDVDVNKDSVGIQT
TNGFAIDTKHVQTQVLVENGGTVVIGGIYTQNERTDINKVPLLGDIPVLGNLFKSTSKINNRTELLVFLTPRVLSDQLSL
K

Sequences:

>Translated_721_residues
MTMRLVKGGLSQATRVARGGAVAWLSLCLFMVAGQAVAQQAAPAAAVSGNTIEKVEQATAGESTVVTVTLKSAPTQKPVE
FSTQQPARIAIDFFGATFAQGRANYQYGGKLLRSANVVQIGDRTRVVLDLSRQTQYKSEVRGNQYVLTLEAAPVAAAVAA
PTFSAPAPVAGAERPAVRNVDFRRGEDLAGRVVVDLSTANSAINIAQQGQNLVVDFVGATLPQSLRRRYDVSDFGTPVQA
MRATDNGTGARLIIEPRGNWQYSSYQTDTQFVVEVRPTKEDPNKLISGPGYRGERLSLNFQNIDVRSLLQVFADFTNLNI
VTSDSVTGTLSLRLKDVPWDQALQIVLDSKGLASRRNGNVLWVAPRGELATKEKAELESQQQVTELEPLRSQVFRLNYQS
AGDVRNMLLGAGAAGGAGGVAGGAATSRILSKRGSLTADTRTNQLFVSDIPSKLEEVQAFLLKIDIPVRQVMIEARIVEA
DDTFSRNLGAKLGFASKTNGAGYGNTYTNVVSPITQGATWDNSPAISFPANGINGVNAASVAVSLFNAGAGRFLALELSA
LEADGRGKIVSSPRVVTADNIKALIEQGTEIPYQQATSSGATSVSFKKANLKLEVTPKITPDGNIFLDVDVNKDSVGIQT
TNGFAIDTKHVQTQVLVENGGTVVIGGIYTQNERTDINKVPLLGDIPVLGNLFKSTSKINNRTELLVFLTPRVLSDQLSL
K
>Mature_720_residues
TMRLVKGGLSQATRVARGGAVAWLSLCLFMVAGQAVAQQAAPAAAVSGNTIEKVEQATAGESTVVTVTLKSAPTQKPVEF
STQQPARIAIDFFGATFAQGRANYQYGGKLLRSANVVQIGDRTRVVLDLSRQTQYKSEVRGNQYVLTLEAAPVAAAVAAP
TFSAPAPVAGAERPAVRNVDFRRGEDLAGRVVVDLSTANSAINIAQQGQNLVVDFVGATLPQSLRRRYDVSDFGTPVQAM
RATDNGTGARLIIEPRGNWQYSSYQTDTQFVVEVRPTKEDPNKLISGPGYRGERLSLNFQNIDVRSLLQVFADFTNLNIV
TSDSVTGTLSLRLKDVPWDQALQIVLDSKGLASRRNGNVLWVAPRGELATKEKAELESQQQVTELEPLRSQVFRLNYQSA
GDVRNMLLGAGAAGGAGGVAGGAATSRILSKRGSLTADTRTNQLFVSDIPSKLEEVQAFLLKIDIPVRQVMIEARIVEAD
DTFSRNLGAKLGFASKTNGAGYGNTYTNVVSPITQGATWDNSPAISFPANGINGVNAASVAVSLFNAGAGRFLALELSAL
EADGRGKIVSSPRVVTADNIKALIEQGTEIPYQQATSSGATSVSFKKANLKLEVTPKITPDGNIFLDVDVNKDSVGIQTT
NGFAIDTKHVQTQVLVENGGTVVIGGIYTQNERTDINKVPLLGDIPVLGNLFKSTSKINNRTELLVFLTPRVLSDQLSLK

Specific function: Essential for the formation of pili. Involved in the biogenesis of type 4 fimbriae probably by serving as a "porthole" allowing passage of the fimbrae through the outer membrane [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=441, Percent_Identity=34.6938775510204, Blast_Score=236, Evalue=6e-63,
Organism=Escherichia coli, GI87082242, Length=390, Percent_Identity=29.7435897435897, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 76910; Mature: 76779

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMRLVKGGLSQATRVARGGAVAWLSLCLFMVAGQAVAQQAAPAAAVSGNTIEKVEQATA
CCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCHHHHHHHHCC
GESTVVTVTLKSAPTQKPVEFSTQQPARIAIDFFGATFAQGRANYQYGGKLLRSANVVQI
CCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHCCCCCHHHCCHHHCCCCEEEE
GDRTRVVLDLSRQTQYKSEVRGNQYVLTLEAAPVAAAVAAPTFSAPAPVAGAERPAVRNV
CCCEEEEEEECHHHHHHHHCCCCEEEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCC
DFRRGEDLAGRVVVDLSTANSAINIAQQGQNLVVDFVGATLPQSLRRRYDVSDFGTPVQA
CCCCCCCCCCEEEEEEECCCCEEEHHHCCCEEEEEEHHCCCHHHHHHHCCCCCCCCCHHE
MRATDNGTGARLIIEPRGNWQYSSYQTDTQFVVEVRPTKEDPNKLISGPGYRGERLSLNF
EEECCCCCCCEEEEECCCCEEECCCCCCCEEEEEEECCCCCCHHHCCCCCCCCCEEEEEE
QNIDVRSLLQVFADFTNLNIVTSDSVTGTLSLRLKDVPWDQALQIVLDSKGLASRRNGNV
CCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEECCCHHHEEEEEECCCCCCCCCCCCE
LWVAPRGELATKEKAELESQQQVTELEPLRSQVFRLNYQSAGDVRNMLLGAGAAGGAGGV
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCCCCCCC
AGGAATSRILSKRGSLTADTRTNQLFVSDIPSKLEEVQAFLLKIDIPVRQVMIEARIVEA
CCHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHHHHHHHHEECCCHHHEEEEEEEEEC
DDTFSRNLGAKLGFASKTNGAGYGNTYTNVVSPITQGATWDNSPAISFPANGINGVNAAS
CCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCHHH
VAVSLFNAGAGRFLALELSALEADGRGKIVSSPRVVTADNIKALIEQGTEIPYQQATSSG
HHHHHHHCCCCEEEEEEEEEECCCCCCCEECCCEEEEHHHHHHHHHCCCCCCHHHHCCCC
ATSVSFKKANLKLEVTPKITPDGNIFLDVDVNKDSVGIQTTNGFAIDTKHVQTQVLVENG
CCEEEEEECCEEEEECCEECCCCCEEEEEECCCCCCCEEECCCEEEECCCCEEEEEEECC
GTVVIGGIYTQNERTDINKVPLLGDIPVLGNLFKSTSKINNRTELLVFLTPRVLSDQLSL
CEEEEEEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCEEEEEEECCHHHCHHHCC
K
C
>Mature Secondary Structure 
TMRLVKGGLSQATRVARGGAVAWLSLCLFMVAGQAVAQQAAPAAAVSGNTIEKVEQATA
CEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCHHHHHHHHCC
GESTVVTVTLKSAPTQKPVEFSTQQPARIAIDFFGATFAQGRANYQYGGKLLRSANVVQI
CCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHCCCCCHHHCCHHHCCCCEEEE
GDRTRVVLDLSRQTQYKSEVRGNQYVLTLEAAPVAAAVAAPTFSAPAPVAGAERPAVRNV
CCCEEEEEEECHHHHHHHHCCCCEEEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCC
DFRRGEDLAGRVVVDLSTANSAINIAQQGQNLVVDFVGATLPQSLRRRYDVSDFGTPVQA
CCCCCCCCCCEEEEEEECCCCEEEHHHCCCEEEEEEHHCCCHHHHHHHCCCCCCCCCHHE
MRATDNGTGARLIIEPRGNWQYSSYQTDTQFVVEVRPTKEDPNKLISGPGYRGERLSLNF
EEECCCCCCCEEEEECCCCEEECCCCCCCEEEEEEECCCCCCHHHCCCCCCCCCEEEEEE
QNIDVRSLLQVFADFTNLNIVTSDSVTGTLSLRLKDVPWDQALQIVLDSKGLASRRNGNV
CCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEECCCHHHEEEEEECCCCCCCCCCCCE
LWVAPRGELATKEKAELESQQQVTELEPLRSQVFRLNYQSAGDVRNMLLGAGAAGGAGGV
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCCCCCCC
AGGAATSRILSKRGSLTADTRTNQLFVSDIPSKLEEVQAFLLKIDIPVRQVMIEARIVEA
CCHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHHHHHHHHEECCCHHHEEEEEEEEEC
DDTFSRNLGAKLGFASKTNGAGYGNTYTNVVSPITQGATWDNSPAISFPANGINGVNAAS
CCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCHHH
VAVSLFNAGAGRFLALELSALEADGRGKIVSSPRVVTADNIKALIEQGTEIPYQQATSSG
HHHHHHHCCCCEEEEEEEEEECCCCCCCEECCCEEEEHHHHHHHHHCCCCCCHHHHCCCC
ATSVSFKKANLKLEVTPKITPDGNIFLDVDVNKDSVGIQTTNGFAIDTKHVQTQVLVENG
CCEEEEEECCEEEEECCEECCCCCEEEEEECCCCCCCEEECCCEEEECCCCEEEEEEECC
GTVVIGGIYTQNERTDINKVPLLGDIPVLGNLFKSTSKINNRTELLVFLTPRVLSDQLSL
CEEEEEEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCEEEEEEECCHHHCHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7901733; 10984043 [H]