Click here to switch to the map view.

The map label for this gene is aroK [H]

Identifier: 187930315

GI number: 187930315

Start: 3397416

End: 3397955

Strand: Reverse

Name: aroK [H]

Synonym: Rpic_3248

Alternate gene names: 187930315

Gene position: 3397955-3397416 (Counterclockwise)

Preceding gene: 187930316

Following gene: 187930314

Centisome position: 86.19

GC content: 64.26

Gene sequence:

>540_bases
ATGGGCGCGGGCAAGACCACGGTCGGCCGGGCGGTGGCGCGTCGGCTCGATCTGCCGTTCTTCGACTCCGACCATGAGAT
CGAAGCCCGCTGCGGCGTGCGCGTGCCAATCATCTTCGAGCACGAGGGCGAAGTGGGCTTCCGCGACCGCGAAACCCACA
TGATCGACGAACTCACCTCGCGCGATGGCGTGGTCGTCGCCACCGGCGGCGGAGCCGTGCTGCGGGCGGAGAACCGCGCG
TTCCTCCGTGAACGCGGCACTGTGATCTACCTGCGCGCCAATCCGCATGACCTGTATCTGCGCACGCGTCACGACAAGAA
CCGGCCGCTGCTGCAGACCGAAAACCCGCGTGCCAAGCTGGAAGAACTGCATGCGGTTCGTGACCCCCTATATCGTGACG
TGGCGCACTTCGTCATCGAAACCGGAAAGCCCACCGTCGCCCAGCTTGTTAATATGGTGCTGATGCAACTCGAGGTGGCT
GGCATTGTCGTACCGCCCGCAGGCGCCTCCACTTCCTCGCAAGTCTCTCCTCAGTCATGA

Upstream 100 bases:

>100_bases
CCGGTTTGTGCAGGATGGTGACCAAATGCCACTACAATCAAGCTCAAATTCCTGATTTCACGCGATTTTGTCTGTTTCGA
ACGTATTTTTCGTCGGCTTG

Downstream 100 bases:

>100_bases
TTACCGTTGATGTTGACCTTGGCGATCGCGCCTATCCGATCCACATTGGCTCGGGGCTGCTGTCCAAGGCCGAGTTGTTC
GCCCCCCACATTCGCGGCGC

Product: shikimate kinase

Products: NA

Alternate protein names: SK [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MGAGKTTVGRAVARRLDLPFFDSDHEIEARCGVRVPIIFEHEGEVGFRDRETHMIDELTSRDGVVVATGGGAVLRAENRA
FLRERGTVIYLRANPHDLYLRTRHDKNRPLLQTENPRAKLEELHAVRDPLYRDVAHFVIETGKPTVAQLVNMVLMQLEVA
GIVVPPAGASTSSQVSPQS

Sequences:

>Translated_179_residues
MGAGKTTVGRAVARRLDLPFFDSDHEIEARCGVRVPIIFEHEGEVGFRDRETHMIDELTSRDGVVVATGGGAVLRAENRA
FLRERGTVIYLRANPHDLYLRTRHDKNRPLLQTENPRAKLEELHAVRDPLYRDVAHFVIETGKPTVAQLVNMVLMQLEVA
GIVVPPAGASTSSQVSPQS
>Mature_178_residues
GAGKTTVGRAVARRLDLPFFDSDHEIEARCGVRVPIIFEHEGEVGFRDRETHMIDELTSRDGVVVATGGGAVLRAENRAF
LRERGTVIYLRANPHDLYLRTRHDKNRPLLQTENPRAKLEELHAVRDPLYRDVAHFVIETGKPTVAQLVNMVLMQLEVAG
IVVPPAGASTSSQVSPQS

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate [H]

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family [H]

Homologues:

Organism=Escherichia coli, GI87082255, Length=159, Percent_Identity=46.5408805031447, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI1786587, Length=148, Percent_Identity=37.8378378378378, Blast_Score=91, Evalue=5e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000623
- InterPro:   IPR023000 [H]

Pfam domain/function: PF01202 SKI [H]

EC number: =2.7.1.71 [H]

Molecular weight: Translated: 19843; Mature: 19712

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAGKTTVGRAVARRLDLPFFDSDHEIEARCGVRVPIIFEHEGEVGFRDRETHMIDELTS
CCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEEEECCCCCCCCCCHHHHHHHHHC
RDGVVVATGGGAVLRAENRAFLRERGTVIYLRANPHDLYLRTRHDKNRPLLQTENPRAKL
CCCEEEEECCCEEEEECCHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCEECCCCHHHH
EELHAVRDPLYRDVAHFVIETGKPTVAQLVNMVLMQLEVAGIVVPPAGASTSSQVSPQS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
GAGKTTVGRAVARRLDLPFFDSDHEIEARCGVRVPIIFEHEGEVGFRDRETHMIDELTS
CCCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEEEECCCCCCCCCCHHHHHHHHHC
RDGVVVATGGGAVLRAENRAFLRERGTVIYLRANPHDLYLRTRHDKNRPLLQTENPRAKL
CCCEEEEECCCEEEEECCHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCEECCCCHHHH
EELHAVRDPLYRDVAHFVIETGKPTVAQLVNMVLMQLEVAGIVVPPAGASTSSQVSPQS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]