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The map label for this gene is hisH [H]

Identifier: 187930292

GI number: 187930292

Start: 3372272

End: 3372925

Strand: Reverse

Name: hisH [H]

Synonym: Rpic_3225

Alternate gene names: 187930292

Gene position: 3372925-3372272 (Counterclockwise)

Preceding gene: 187930293

Following gene: 187930291

Centisome position: 85.55

GC content: 62.54

Gene sequence:

>654_bases
ATGACTAAGATCGCAATCGTCGATTACGGCATGGGCAATCTGCGCTCGGTGGCGCAAGCCCTGATGGCCGTTGCTCCGGA
GGCCGATGTGCGCATCAGTGCGCAAGCGGATGAAATTCGTTCGGCCGACCGTGTGGTGCTGCCAGGGCAGGGCGCCATGC
CCGACTGCATGGCCGCGTTCGACCAGTCCGGCCTGCGCGATGCTGTGCTGGAGGCATCGCGCAGCAAGCCGATGCTGGGC
GTATGTGTGGGCGAGCAGATGCTGCTCGAGCGCAGCACCGAGGCGCGCCCCGGGCAGCCGTACACGGAAGGGTTGGGCCT
CATCAAGGGCGATGTGGTCCGCTTTGAGCTGGACGGCCAGTTGCAGCCGGACGGTTCACGGTACAAAGTGCCTCAGATGG
GCTGGAACCGCGTGCACCAGAGTCGTGCCCACGCCCTTTGGGAAGGTGTGCCGGACCAGAGCTACTTCTATTTCGTGCAC
AGTTACTACGCGCGGCCCGCCAATGCCGACGAATCGGTCGGAGAGACCGAATACGGCGTGCGGTTTACCTCCGCCATCGC
CCGGGATAATATTTTTGCAACACAGTTTCACCCGGAAAAAAGTGCGCAAGCCGGGCTGCAGATCTACCGGAACTTCGTGC
ACTGGAACCCGTGA

Upstream 100 bases:

>100_bases
GTACGCGGTTGATGGGCTTGATCCTGGCGGCGCTTGCCGTGGAATTCATCGTTGACGGGCTGAAAACCCTGTTGCCCGTA
CTTAGGGCCTAAGTGAAGTC

Downstream 100 bases:

>100_bases
AATCGGGCTCACAGTCGCGACCGAATCCCTTTTGCCCGTTTAACCTCACCCCCTCAACTTTGTCGTTACGACTATGCTGC
TCATCCCGGCCATCGACCTG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTKIAIVDYGMGNLRSVAQALMAVAPEADVRISAQADEIRSADRVVLPGQGAMPDCMAAFDQSGLRDAVLEASRSKPMLG
VCVGEQMLLERSTEARPGQPYTEGLGLIKGDVVRFELDGQLQPDGSRYKVPQMGWNRVHQSRAHALWEGVPDQSYFYFVH
SYYARPANADESVGETEYGVRFTSAIARDNIFATQFHPEKSAQAGLQIYRNFVHWNP

Sequences:

>Translated_217_residues
MTKIAIVDYGMGNLRSVAQALMAVAPEADVRISAQADEIRSADRVVLPGQGAMPDCMAAFDQSGLRDAVLEASRSKPMLG
VCVGEQMLLERSTEARPGQPYTEGLGLIKGDVVRFELDGQLQPDGSRYKVPQMGWNRVHQSRAHALWEGVPDQSYFYFVH
SYYARPANADESVGETEYGVRFTSAIARDNIFATQFHPEKSAQAGLQIYRNFVHWNP
>Mature_216_residues
TKIAIVDYGMGNLRSVAQALMAVAPEADVRISAQADEIRSADRVVLPGQGAMPDCMAAFDQSGLRDAVLEASRSKPMLGV
CVGEQMLLERSTEARPGQPYTEGLGLIKGDVVRFELDGQLQPDGSRYKVPQMGWNRVHQSRAHALWEGVPDQSYFYFVHS
YYARPANADESVGETEYGVRFTSAIARDNIFATQFHPEKSAQAGLQIYRNFVHWNP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=212, Percent_Identity=36.3207547169811, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=223, Percent_Identity=32.2869955156951, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 24026; Mature: 23895

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIAIVDYGMGNLRSVAQALMAVAPEADVRISAQADEIRSADRVVLPGQGAMPDCMAAF
CCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCHHHHCCCCEEEECCCCCCHHHHHHH
DQSGLRDAVLEASRSKPMLGVCVGEQMLLERSTEARPGQPYTEGLGLIKGDVVRFELDGQ
CCCCHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCHHHCCCCEECCEEEEEECCE
LQPDGSRYKVPQMGWNRVHQSRAHALWEGVPDQSYFYFVHSYYARPANADESVGETEYGV
ECCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCCCCCCCCCCCHHCC
RFTSAIARDNIFATQFHPEKSAQAGLQIYRNFVHWNP
HHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKIAIVDYGMGNLRSVAQALMAVAPEADVRISAQADEIRSADRVVLPGQGAMPDCMAAF
CEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCHHHHCCCCEEEECCCCCCHHHHHHH
DQSGLRDAVLEASRSKPMLGVCVGEQMLLERSTEARPGQPYTEGLGLIKGDVVRFELDGQ
CCCCHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCHHHCCCCEECCEEEEEECCE
LQPDGSRYKVPQMGWNRVHQSRAHALWEGVPDQSYFYFVHSYYARPANADESVGETEYGV
ECCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCCCCCCCCCCCHHCC
RFTSAIARDNIFATQFHPEKSAQAGLQIYRNFVHWNP
HHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]