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The map label for this gene is hisI

Identifier: 187930289

GI number: 187930289

Start: 3370212

End: 3370634

Strand: Reverse

Name: hisI

Synonym: Rpic_3222

Alternate gene names: 187930289

Gene position: 3370634-3370212 (Counterclockwise)

Preceding gene: 187930290

Following gene: 187930288

Centisome position: 85.49

GC content: 60.99

Gene sequence:

>423_bases
ATGAGCAAGAAGTGGCTGAACAAGGTCCACTGGGACGACAACGGCCTGGTGCCGGTGATCGTGCAGGAGCAGGGCAGCAA
TGACGTCCTGATGTTCGCGTTCATGAACCGCGAGGCGCTGCAGAAGACGGTTGAAAGCGGCGAGGCCGTGTTCTGGTCGC
GCTCGCGCAAGCGCCTGTGGCACAAGGGCGAGGAGTCCGGCCACATCCAGAAGGTGCACGAGATCCGCCTCGACTGCGAT
GAAGACGTGATCTTGCTCCGCGTCACACAAATCGGCGGCATCGCCTGCCATACTGGCCGCCACGCGTGCTTTTTCCAGAA
GTTCGAAGGCAATGTGGAAGACGGCGATTGGCACACCGTCGAGCCGGTGCTCAAGAACCCCGACGACATTTACGCCGGCA
AGCCCGGCCACAATCATGAGTGA

Upstream 100 bases:

>100_bases
ATGCGGTGCTGGCCGCCAGCATCTTCCACTACGGCGAATACACCGTTGGCCAGGCCAAGCAATTCATGGCAGAGCAAGGC
ATTCCAGTACGGATCGGATC

Downstream 100 bases:

>100_bases
CACCCTGCGCCGCCTGGGCGAGGTGCTCGAATCCCGCAAGCTCGCCAACGGCGGTGACCCGGAAAAGAGCTACATCGCGC
GCCTGTTCAACAAGGGCGAC

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MSKKWLNKVHWDDNGLVPVIVQEQGSNDVLMFAFMNREALQKTVESGEAVFWSRSRKRLWHKGEESGHIQKVHEIRLDCD
EDVILLRVTQIGGIACHTGRHACFFQKFEGNVEDGDWHTVEPVLKNPDDIYAGKPGHNHE

Sequences:

>Translated_140_residues
MSKKWLNKVHWDDNGLVPVIVQEQGSNDVLMFAFMNREALQKTVESGEAVFWSRSRKRLWHKGEESGHIQKVHEIRLDCD
EDVILLRVTQIGGIACHTGRHACFFQKFEGNVEDGDWHTVEPVLKNPDDIYAGKPGHNHE
>Mature_139_residues
SKKWLNKVHWDDNGLVPVIVQEQGSNDVLMFAFMNREALQKTVESGEAVFWSRSRKRLWHKGEESGHIQKVHEIRLDCDE
DVILLRVTQIGGIACHTGRHACFFQKFEGNVEDGDWHTVEPVLKNPDDIYAGKPGHNHE

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=91, Percent_Identity=50.5494505494505, Blast_Score=97, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6319819, Length=105, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_RALPJ (B2UEE4)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001900776.1
- ProteinModelPortal:   B2UEE4
- SMR:   B2UEE4
- GeneID:   6286063
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_3222
- HOGENOM:   HBG294308
- OMA:   LWLKGES
- ProtClustDB:   PRK00051
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 16101; Mature: 15970

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKWLNKVHWDDNGLVPVIVQEQGSNDVLMFAFMNREALQKTVESGEAVFWSRSRKRLW
CCCCCCCEEEECCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHHCCCEEEEECHHHHHH
HKGEESGHIQKVHEIRLDCDEDVILLRVTQIGGIACHTGRHACFFQKFEGNVEDGDWHTV
CCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCEEEECCCCEEEEEECCCCCCCCCCEEE
EPVLKNPDDIYAGKPGHNHE
CHHHCCCCCEECCCCCCCCC
>Mature Secondary Structure 
SKKWLNKVHWDDNGLVPVIVQEQGSNDVLMFAFMNREALQKTVESGEAVFWSRSRKRLW
CCCCCCEEEECCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHHCCCEEEEECHHHHHH
HKGEESGHIQKVHEIRLDCDEDVILLRVTQIGGIACHTGRHACFFQKFEGNVEDGDWHTV
CCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCEEEECCCCEEEEEECCCCCCCCCCEEE
EPVLKNPDDIYAGKPGHNHE
CHHHCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA