Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 187930290

GI number: 187930290

Start: 3370631

End: 3371407

Strand: Reverse

Name: hisF

Synonym: Rpic_3223

Alternate gene names: 187930290

Gene position: 3371407-3370631 (Counterclockwise)

Preceding gene: 187930291

Following gene: 187930289

Centisome position: 85.51

GC content: 65.77

Gene sequence:

>777_bases
ATGCTAGCCAAACGAATCATCCCCTGCCTGGACGTGACCAACGGCCGGGTGGTCAAGGGCGTCAACTTCGTCGAATTGCG
CGACGCCGGCGACCCCGTCGAAATCGCACGCCGCTACGACGAGCAGGGCGCCGACGAAATCACCTTCCTCGACATCACCG
CCACGTCGGACGGCCGCGACCTGATGCTGGGCATCATTGAAGCGGTGGCGTCGCAGGTGTTCATTCCGCTGACGGTGGGC
GGCGGTGTGCGTGCGCTGGAAGATGTGCGACGGCTGCTCAATGCCGGTGCGGACAAGATCAGCATGAACTCCTCGGCGGT
GGCGAATCCGCAGCTCGTGTCGGACGCCAGTGCCCGCCATGGCGCGCAGTGCATCGTCGTGGCTATCGACGCCAAGAAGG
TGTCGGCCGAAGGCGAAGCGCCGCGCTGGGAAGTCTTCACGCACGGCGGCCGAAAAGGCACGGGCCTGGACGCCGTGGCA
TGGGCGCGCGAAATGGCGCAGCGCGGCGCCGGCGAAATCCTGCTCACCAGCATGGACCGCGACGGCACGAAAGCCGGCTT
CGACCTGGAACTCACGCGCGCCGTGTCGGACGCAGTGCCGGTGCCGGTCATCGCCTCGGGCGGCGTGGGCAACCTGCAGC
ATCTGGCTGACGGCATTCAGCGAGGTCACGCCGATGCGGTGCTGGCCGCCAGCATCTTCCACTACGGCGAATACACCGTT
GGCCAGGCCAAGCAATTCATGGCAGAGCAAGGCATTCCAGTACGGATCGGATCATGA

Upstream 100 bases:

>100_bases
ACCTGAACTTCAAGGAAGCACAAGCCCTCGCGGACAAACTCGGCGCGGCCTGATCGTTGATCCAGCCGTGCACAACGGGC
GCTCCGGCGCCCGACTCATC

Downstream 100 bases:

>100_bases
GCAAGAAGTGGCTGAACAAGGTCCACTGGGACGACAACGGCCTGGTGCCGGTGATCGTGCAGGAGCAGGGCAGCAATGAC
GTCCTGATGTTCGCGTTCAT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRDLMLGIIEAVASQVFIPLTVG
GGVRALEDVRRLLNAGADKISMNSSAVANPQLVSDASARHGAQCIVVAIDAKKVSAEGEAPRWEVFTHGGRKGTGLDAVA
WAREMAQRGAGEILLTSMDRDGTKAGFDLELTRAVSDAVPVPVIASGGVGNLQHLADGIQRGHADAVLAASIFHYGEYTV
GQAKQFMAEQGIPVRIGS

Sequences:

>Translated_258_residues
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRDLMLGIIEAVASQVFIPLTVG
GGVRALEDVRRLLNAGADKISMNSSAVANPQLVSDASARHGAQCIVVAIDAKKVSAEGEAPRWEVFTHGGRKGTGLDAVA
WAREMAQRGAGEILLTSMDRDGTKAGFDLELTRAVSDAVPVPVIASGGVGNLQHLADGIQRGHADAVLAASIFHYGEYTV
GQAKQFMAEQGIPVRIGS
>Mature_258_residues
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRDLMLGIIEAVASQVFIPLTVG
GGVRALEDVRRLLNAGADKISMNSSAVANPQLVSDASARHGAQCIVVAIDAKKVSAEGEAPRWEVFTHGGRKGTGLDAVA
WAREMAQRGAGEILLTSMDRDGTKAGFDLELTRAVSDAVPVPVIASGGVGNLQHLADGIQRGHADAVLAASIFHYGEYTV
GQAKQFMAEQGIPVRIGS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=44.2307692307692, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=30.1652892561983, Blast_Score=94, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=35.6687898089172, Blast_Score=171, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_RALPJ (B2UEE5)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001900777.1
- ProteinModelPortal:   B2UEE5
- SMR:   B2UEE5
- GeneID:   6286064
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_3223
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27201; Mature: 27201

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRD
CCHHHCCCCEECCCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCHH
LMLGIIEAVASQVFIPLTVGGGVRALEDVRRLLNAGADKISMNSSAVANPQLVSDASARH
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCCCC
GAQCIVVAIDAKKVSAEGEAPRWEVFTHGGRKGTGLDAVAWAREMAQRGAGEILLTSMDR
CCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCC
DGTKAGFDLELTRAVSDAVPVPVIASGGVGNLQHLADGIQRGHADAVLAASIFHYGEYTV
CCCCCCCCEEHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCH
GQAKQFMAEQGIPVRIGS
HHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRD
CCHHHCCCCEECCCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCHH
LMLGIIEAVASQVFIPLTVGGGVRALEDVRRLLNAGADKISMNSSAVANPQLVSDASARH
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCCCC
GAQCIVVAIDAKKVSAEGEAPRWEVFTHGGRKGTGLDAVAWAREMAQRGAGEILLTSMDR
CCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCC
DGTKAGFDLELTRAVSDAVPVPVIASGGVGNLQHLADGIQRGHADAVLAASIFHYGEYTV
CCCCCCCCEEHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCH
GQAKQFMAEQGIPVRIGS
HHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA