| Definition | Ralstonia pickettii 12J chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010682 |
| Length | 3,942,557 |
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The map label for this gene is pcrA [H]
Identifier: 187930241
GI number: 187930241
Start: 3326681
End: 3328354
Strand: Reverse
Name: pcrA [H]
Synonym: Rpic_3174
Alternate gene names: 187930241
Gene position: 3328354-3326681 (Counterclockwise)
Preceding gene: 187930242
Following gene: 187930240
Centisome position: 84.42
GC content: 55.62
Gene sequence:
>1674_bases GTGATAGCCGTCGATGCCTGGCAGCCCGCAGATGGGCTGACGCTTGAACCCAACGCCCTGCTTGCGGCCAAGGAGCAAGT GCGCAGCCTTGCACTGACAGCAGGCCCTGGTGCGGGCAAGACTGAAATGCTTGCGCAGCGGGCAGACTTCCTGCTGCGCA CAGGCACCTGTTGCTATCCCAAACGAATTCTGGCCATCTCGTTCAAGGTCGATGCCAGCAGCAACCTGAAAGAGCGAGTC AAGCGACGCTGTGGTTCAGAAATGGCAGCGCGGTTTGACAGCTACACGTTTCACGCATTTGCCAAACGCATCATCGACCG CTTTCGACCCGTCCTAACAGGGCAAGATGCTCTGGATGTCGGCTACAAGATCGTTGAGCGAAAAGTGAAGCCGTCCCGCC AGGAAATCGAGTTTGCAGACCTTGTTCCTCTGGCAATCAAGATCCTACGGAAGTCAGCTATGGCGCGGAACGCTGTCCGC CAAACCTACAGCGACGTTTTTCTCGACGAATTCCAGGACTGCACCAACCTGCAGTACGAGCTGCTCAAGCTCGCCTTTCA AGGTACGAGCATTCGCCTGACCGCCGTGGGTGACACAAAGCAGAAGATCATGGGTTGGGCAGGGGCCATGGACGGCATCT TCCAGGCTTTCGTTGCCGATTTTGCGGCCACACCGCTCAACATGTACCGCAACTTTCGTTCGAAGCCGCGACTGCTGCGC CTGCAGAACGAGATCGTTCGCAGGCTTGACCCCTCATCTGTCATGCCCAATGAGCAGCTTGCCGGCGACGAAGGCGAGGT ATTCGCGTGGCGCTTCGATGACAGTCGCCAGGAAGCTGAACATCTCGCAGAGCTGATCGATGGTTGGATAAAGACTGAGC AGCTGCCTCCAGCAGAGATTGCAGTCCTCATTCGCAATCAGCTTGATTTGTACGCCGATCATCTTATGGCGGCACTGGAG GCTCGCGGCATCCCTTACCGCAATGAGCAGCGGCTGCAAGACATCACGGTTGAGCCTGCAGCCCGCTTGATCGTGGATTA TCTGACATGCCTCTACGGCCAGCGCGAACCAAAAGCATGGATTCGGCTCATGAATCAGCTGATCCCGTTTGCTGATGAAG AGATTCAGTCGAACGCGCGCAAGGACCTTGATCGGCTCATCAAGCAACAACGAAAAGAGGTGGTCATTGCCGAGTTGGTC GCAGAGCCCTTTTCAGGCTGGTGGGAGTTTGTTCGCGCCTTCCTGAAGCAGGTCAGTATCGAAACACTAGTGTCGCTTTC CCCAGACTACGAGTCGCATGGCCGGCTGAAAGAGGTTGTCCGTGACACCAAGATCCGGATCGAGGAATTGCTGAAACTGG AGCCTGATCTGCCTGAAGCCCTTGAGCGCTTTTCTGACGATCAGGCAGTACGAATCCTGACCATTCATAAGAGTAAGGGC CTTGAGTTCGATTCGGTCGTCATCATGGCGGTCGAAAAAGAAATCTTCTTTGGCGATCAGGACGAAAACCGCTGTGCCTT TTTTGTCGGGGTTTCTCGAGCCAAGAGGCGACTAGTCCTTACCCATGCAGATCAACGGGAGCGCCCAGCAGGACATACGA GACGCTGGGATGTGCAGCGCACGGCTCAAACTGAGTATTTCGGCTATGCCATGCCGTTCGTGAGACAAGAATAA
Upstream 100 bases:
>100_bases CGCTCGCCAAGCTCAGCGATGCGGACCTGCTCGCGAACATGCCCGAATCACTTGGACGCTTGGCCGATGCCGTCATCGCC AAGCTCGCGGAGTTGCCGGA
Downstream 100 bases:
>100_bases TGAACAACAGGACATTGAAGCCCGGCGGGCGTTTGGTGAAATTCGGCGACGTGGTGCGCCTCTCGAAAGCCCGCAGCCAA GATCCGCTGGCCGATGGCAT
Product: UvrD/REP helicase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 557; Mature: 557
Protein sequence:
>557_residues MIAVDAWQPADGLTLEPNALLAAKEQVRSLALTAGPGAGKTEMLAQRADFLLRTGTCCYPKRILAISFKVDASSNLKERV KRRCGSEMAARFDSYTFHAFAKRIIDRFRPVLTGQDALDVGYKIVERKVKPSRQEIEFADLVPLAIKILRKSAMARNAVR QTYSDVFLDEFQDCTNLQYELLKLAFQGTSIRLTAVGDTKQKIMGWAGAMDGIFQAFVADFAATPLNMYRNFRSKPRLLR LQNEIVRRLDPSSVMPNEQLAGDEGEVFAWRFDDSRQEAEHLAELIDGWIKTEQLPPAEIAVLIRNQLDLYADHLMAALE ARGIPYRNEQRLQDITVEPAARLIVDYLTCLYGQREPKAWIRLMNQLIPFADEEIQSNARKDLDRLIKQQRKEVVIAELV AEPFSGWWEFVRAFLKQVSIETLVSLSPDYESHGRLKEVVRDTKIRIEELLKLEPDLPEALERFSDDQAVRILTIHKSKG LEFDSVVIMAVEKEIFFGDQDENRCAFFVGVSRAKRRLVLTHADQRERPAGHTRRWDVQRTAQTEYFGYAMPFVRQE
Sequences:
>Translated_557_residues MIAVDAWQPADGLTLEPNALLAAKEQVRSLALTAGPGAGKTEMLAQRADFLLRTGTCCYPKRILAISFKVDASSNLKERV KRRCGSEMAARFDSYTFHAFAKRIIDRFRPVLTGQDALDVGYKIVERKVKPSRQEIEFADLVPLAIKILRKSAMARNAVR QTYSDVFLDEFQDCTNLQYELLKLAFQGTSIRLTAVGDTKQKIMGWAGAMDGIFQAFVADFAATPLNMYRNFRSKPRLLR LQNEIVRRLDPSSVMPNEQLAGDEGEVFAWRFDDSRQEAEHLAELIDGWIKTEQLPPAEIAVLIRNQLDLYADHLMAALE ARGIPYRNEQRLQDITVEPAARLIVDYLTCLYGQREPKAWIRLMNQLIPFADEEIQSNARKDLDRLIKQQRKEVVIAELV AEPFSGWWEFVRAFLKQVSIETLVSLSPDYESHGRLKEVVRDTKIRIEELLKLEPDLPEALERFSDDQAVRILTIHKSKG LEFDSVVIMAVEKEIFFGDQDENRCAFFVGVSRAKRRLVLTHADQRERPAGHTRRWDVQRTAQTEYFGYAMPFVRQE >Mature_557_residues MIAVDAWQPADGLTLEPNALLAAKEQVRSLALTAGPGAGKTEMLAQRADFLLRTGTCCYPKRILAISFKVDASSNLKERV KRRCGSEMAARFDSYTFHAFAKRIIDRFRPVLTGQDALDVGYKIVERKVKPSRQEIEFADLVPLAIKILRKSAMARNAVR QTYSDVFLDEFQDCTNLQYELLKLAFQGTSIRLTAVGDTKQKIMGWAGAMDGIFQAFVADFAATPLNMYRNFRSKPRLLR LQNEIVRRLDPSSVMPNEQLAGDEGEVFAWRFDDSRQEAEHLAELIDGWIKTEQLPPAEIAVLIRNQLDLYADHLMAALE ARGIPYRNEQRLQDITVEPAARLIVDYLTCLYGQREPKAWIRLMNQLIPFADEEIQSNARKDLDRLIKQQRKEVVIAELV AEPFSGWWEFVRAFLKQVSIETLVSLSPDYESHGRLKEVVRDTKIRIEELLKLEPDLPEALERFSDDQAVRILTIHKSKG LEFDSVVIMAVEKEIFFGDQDENRCAFFVGVSRAKRRLVLTHADQRERPAGHTRRWDVQRTAQTEYFGYAMPFVRQE
Specific function: Essential helicase [H]
COG id: COG0210
COG function: function code L; Superfamily I DNA and RNA helicases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]
Homologues:
Organism=Escherichia coli, GI2367296, Length=620, Percent_Identity=22.4193548387097, Blast_Score=103, Evalue=2e-23,
Paralogues:
None
Copy number: 3000 [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005751 - InterPro: IPR013986 - InterPro: IPR014017 - InterPro: IPR000212 - InterPro: IPR014016 [H]
Pfam domain/function: PF00580 UvrD-helicase [H]
EC number: =3.6.4.12 [H]
Molecular weight: Translated: 63804; Mature: 63804
Theoretical pI: Translated: 7.16; Mature: 7.16
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIAVDAWQPADGLTLEPNALLAAKEQVRSLALTAGPGAGKTEMLAQRADFLLRTGTCCYP CEEECCCCCCCCCEECCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCC KRILAISFKVDASSNLKERVKRRCGSEMAARFDSYTFHAFAKRIIDRFRPVLTGQDALDV CEEEEEEEEECCCCCHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHH GYKIVERKVKPSRQEIEFADLVPLAIKILRKSAMARNAVRQTYSDVFLDEFQDCTNLQYE HHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH LLKLAFQGTSIRLTAVGDTKQKIMGWAGAMDGIFQAFVADFAATPLNMYRNFRSKPRLLR HHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHH LQNEIVRRLDPSSVMPNEQLAGDEGEVFAWRFDDSRQEAEHLAELIDGWIKTEQLPPAEI HHHHHHHHCCCCCCCCCHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHH AVLIRNQLDLYADHLMAALEARGIPYRNEQRLQDITVEPAARLIVDYLTCLYGQREPKAW HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHH IRLMNQLIPFADEEIQSNARKDLDRLIKQQRKEVVIAELVAEPFSGWWEFVRAFLKQVSI HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ETLVSLSPDYESHGRLKEVVRDTKIRIEELLKLEPDLPEALERFSDDQAVRILTIHKSKG HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEEECCCC LEFDSVVIMAVEKEIFFGDQDENRCAFFVGVSRAKRRLVLTHADQRERPAGHTRRWDVQR CCCCCEEEEEEEHHHCCCCCCCCCEEEEEEHHHHHCEEEEEECCCCCCCCCCCCCCCCHH TAQTEYFGYAMPFVRQE HHHHHHHHHHCCHHCCC >Mature Secondary Structure MIAVDAWQPADGLTLEPNALLAAKEQVRSLALTAGPGAGKTEMLAQRADFLLRTGTCCYP CEEECCCCCCCCCEECCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCC KRILAISFKVDASSNLKERVKRRCGSEMAARFDSYTFHAFAKRIIDRFRPVLTGQDALDV CEEEEEEEEECCCCCHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHH GYKIVERKVKPSRQEIEFADLVPLAIKILRKSAMARNAVRQTYSDVFLDEFQDCTNLQYE HHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH LLKLAFQGTSIRLTAVGDTKQKIMGWAGAMDGIFQAFVADFAATPLNMYRNFRSKPRLLR HHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHH LQNEIVRRLDPSSVMPNEQLAGDEGEVFAWRFDDSRQEAEHLAELIDGWIKTEQLPPAEI HHHHHHHHCCCCCCCCCHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHH AVLIRNQLDLYADHLMAALEARGIPYRNEQRLQDITVEPAARLIVDYLTCLYGQREPKAW HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHH IRLMNQLIPFADEEIQSNARKDLDRLIKQQRKEVVIAELVAEPFSGWWEFVRAFLKQVSI HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ETLVSLSPDYESHGRLKEVVRDTKIRIEELLKLEPDLPEALERFSDDQAVRILTIHKSKG HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEEECCCC LEFDSVVIMAVEKEIFFGDQDENRCAFFVGVSRAKRRLVLTHADQRERPAGHTRRWDVQR CCCCCEEEEEEEHHHCCCCCCCCCEEEEEEHHHHHCEEEEEECCCCCCCCCCCCCCCCHH TAQTEYFGYAMPFVRQE HHHHHHHHHHCCHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA