| Definition | Ralstonia pickettii 12J chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010682 |
| Length | 3,942,557 |
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The map label for this gene is murF [H]
Identifier: 187930161
GI number: 187930161
Start: 3230744
End: 3232180
Strand: Reverse
Name: murF [H]
Synonym: Rpic_3093
Alternate gene names: 187930161
Gene position: 3232180-3230744 (Counterclockwise)
Preceding gene: 187930162
Following gene: 187930160
Centisome position: 81.98
GC content: 67.36
Gene sequence:
>1437_bases ATGAGCGCCGCGACCGTGATGATGCGCGCCACCGACGCCGCTGCCTGGATGACCGGCGCCCATCTGGCCGGCCCCGATGC GGCGATCCTGCGCGTGACGACCGACAGCCGCGCCGTCCAGCCGGGCGACCTGTTCGTCGCGCTGATCGGCGAGCGTTTCG ATGCGCACGATTTCCTGCCCGAGGTGGTGGCGCGTGGCGCTGCTGCCGTGCTGGTGTCGCGCGCACCGGCTGCCACCCTG GATGTCACCAATGTGGCCGTGCTGACTGTCGCCGACACGCGCTTCGGGCTCGGCCAGCTTGCCGCCGGCTGGCGCCGCCA GTTCCCGATTCCCGTCGTCGCCGTGACGGGCAGCAACGGCAAGACCACGGTCAAGGAGATGATCTCCGCGATCTTCGCGC AGGCCGTGGGTGAAGACGCCCGCCTGGCGACCGCCGGCAATTTCAACAACGACATCGGCCTGCCGCTCACGCTGTTCCGC CTGAACGCGCAACATAAGCTGGCCGTGCTTGAACTCGGCATGAACCATCCGGGCGAAACGGCTGTATTGGCCGCCATTGC CCAGCCGACCGTGGCGATGATCAACAACGCGCAGCGCGAGCACCAGGAATTCATGGTCAGCGTGGAAGCGGTGGCTGAAG AACACGCCGCTGTGCTGGCCGCGTTGCCGGCCAATGGCGTGGCGGTGTATCCGCGCGATGCGGCCAACGGTGGCGAATAT GCGCCGGTGTGGAAAGCCGCGGCGGGCTCGCGGCGCGTGCTCGATTTCGGTATGGATGCCGGGGCCGTGACGGGCACCGT GGTGGACACCGCCGACGGGCAACGCATTGAAGTGCAGGCTCCGGGCCAGCGTTTCGTCATCACCTTGCCGCTGCTGGGCC TGCATAACGCACGCAATGCGCTGGCGGCGACGGCGTGCGCGCTGGCAGCCGGTGTCGCTCCCGAGGTGATTGCGCAAGCG CTGGGCAACTTTGCACCCGTCAAGGGGCGGTTGCAGCGCAAGCAGGGCACGCATGGCGGCCTGGTCATCGACGACACGTA CAACGCGAACCCGGATTCGATGCGCGCCGCGATTGACGCGCTTGTCACGCTGCCCGCGCCGCGCTGGCTGGTGCTGGGTG ACATGGGCGAGGTGGGCTCTAAGGGGCCGGCATTCCACGAAGAGATCGGGGCCTACGCGCGCGCGCGCGGCATCGACGCA GTGCTGGCAACCGGCGAGCTTGCGCGCCACACGGTCGATGCCTTCGGCCCGGGCGCACAGCATTTTGCGTCGGCGGAAGA CTTGATTGAACACGGTGTGCCCGCCATTGCGCCGGCCGCGACGGTATTGGTGAAGGGATCGCGCTTCATGCGGATGGAAC GCATTGTGGAAGCGCTGGTCTTGAAAGACCCGGTAGCGGATTCGCCAGCCGGTTCCAACACACAACAGCAGCATTAA
Upstream 100 bases:
>100_bases GCAAGGGGCATGAATCGACGCAGGAGATTGCCGGTCGCAAGCGGCCGTTCTCCGATCAGGAACACGTGCGCCTGGCACTC GCCGCCCGCGGGGTGAATGC
Downstream 100 bases:
>100_bases GGGAAGCAAGAACCCATGTTATTGGCATTGGCGCAATGGTTGCAGAACGACTACGGCTTCCTGCGCGTCTTCAACTATCT GACGTTCCGCGCCGTGATGG
Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate/D-alanyl-D-alanyl ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 478; Mature: 477
Protein sequence:
>478_residues MSAATVMMRATDAAAWMTGAHLAGPDAAILRVTTDSRAVQPGDLFVALIGERFDAHDFLPEVVARGAAAVLVSRAPAATL DVTNVAVLTVADTRFGLGQLAAGWRRQFPIPVVAVTGSNGKTTVKEMISAIFAQAVGEDARLATAGNFNNDIGLPLTLFR LNAQHKLAVLELGMNHPGETAVLAAIAQPTVAMINNAQREHQEFMVSVEAVAEEHAAVLAALPANGVAVYPRDAANGGEY APVWKAAAGSRRVLDFGMDAGAVTGTVVDTADGQRIEVQAPGQRFVITLPLLGLHNARNALAATACALAAGVAPEVIAQA LGNFAPVKGRLQRKQGTHGGLVIDDTYNANPDSMRAAIDALVTLPAPRWLVLGDMGEVGSKGPAFHEEIGAYARARGIDA VLATGELARHTVDAFGPGAQHFASAEDLIEHGVPAIAPAATVLVKGSRFMRMERIVEALVLKDPVADSPAGSNTQQQH
Sequences:
>Translated_478_residues MSAATVMMRATDAAAWMTGAHLAGPDAAILRVTTDSRAVQPGDLFVALIGERFDAHDFLPEVVARGAAAVLVSRAPAATL DVTNVAVLTVADTRFGLGQLAAGWRRQFPIPVVAVTGSNGKTTVKEMISAIFAQAVGEDARLATAGNFNNDIGLPLTLFR LNAQHKLAVLELGMNHPGETAVLAAIAQPTVAMINNAQREHQEFMVSVEAVAEEHAAVLAALPANGVAVYPRDAANGGEY APVWKAAAGSRRVLDFGMDAGAVTGTVVDTADGQRIEVQAPGQRFVITLPLLGLHNARNALAATACALAAGVAPEVIAQA LGNFAPVKGRLQRKQGTHGGLVIDDTYNANPDSMRAAIDALVTLPAPRWLVLGDMGEVGSKGPAFHEEIGAYARARGIDA VLATGELARHTVDAFGPGAQHFASAEDLIEHGVPAIAPAATVLVKGSRFMRMERIVEALVLKDPVADSPAGSNTQQQH >Mature_477_residues SAATVMMRATDAAAWMTGAHLAGPDAAILRVTTDSRAVQPGDLFVALIGERFDAHDFLPEVVARGAAAVLVSRAPAATLD VTNVAVLTVADTRFGLGQLAAGWRRQFPIPVVAVTGSNGKTTVKEMISAIFAQAVGEDARLATAGNFNNDIGLPLTLFRL NAQHKLAVLELGMNHPGETAVLAAIAQPTVAMINNAQREHQEFMVSVEAVAEEHAAVLAALPANGVAVYPRDAANGGEYA PVWKAAAGSRRVLDFGMDAGAVTGTVVDTADGQRIEVQAPGQRFVITLPLLGLHNARNALAATACALAAGVAPEVIAQAL GNFAPVKGRLQRKQGTHGGLVIDDTYNANPDSMRAAIDALVTLPAPRWLVLGDMGEVGSKGPAFHEEIGAYARARGIDAV LATGELARHTVDAFGPGAQHFASAEDLIEHGVPAIAPAATVLVKGSRFMRMERIVEALVLKDPVADSPAGSNTQQQH
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=444, Percent_Identity=42.5675675675676, Blast_Score=279, Evalue=2e-76,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 49671; Mature: 49540
Theoretical pI: Translated: 6.05; Mature: 6.05
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSAATVMMRATDAAAWMTGAHLAGPDAAILRVTTDSRAVQPGDLFVALIGERFDAHDFLP CCCCEEEEEECCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHH EVVARGAAAVLVSRAPAATLDVTNVAVLTVADTRFGLGQLAAGWRRQFPIPVVAVTGSNG HHHHCCHHEEEEECCCCCEEEECCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCC KTTVKEMISAIFAQAVGEDARLATAGNFNNDIGLPLTLFRLNAQHKLAVLELGMNHPGET CHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCH AVLAAIAQPTVAMINNAQREHQEFMVSVEAVAEEHAAVLAALPANGVAVYPRDAANGGEY HHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEECCCCCCCCCCC APVWKAAAGSRRVLDFGMDAGAVTGTVVDTADGQRIEVQAPGQRFVITLPLLGLHNARNA CCHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCHHHH LAATACALAAGVAPEVIAQALGNFAPVKGRLQRKQGTHGGLVIDDTYNANPDSMRAAIDA HHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHH LVTLPAPRWLVLGDMGEVGSKGPAFHEEIGAYARARGIDAVLATGELARHTVDAFGPGAQ HHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHEECCCHHHHHHHHHCCCCHH HFASAEDLIEHGVPAIAPAATVLVKGSRFMRMERIVEALVLKDPVADSPAGSNTQQQH HHCCHHHHHHCCCCCCCCHHHEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC >Mature Secondary Structure SAATVMMRATDAAAWMTGAHLAGPDAAILRVTTDSRAVQPGDLFVALIGERFDAHDFLP CCCEEEEEECCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHH EVVARGAAAVLVSRAPAATLDVTNVAVLTVADTRFGLGQLAAGWRRQFPIPVVAVTGSNG HHHHCCHHEEEEECCCCCEEEECCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCC KTTVKEMISAIFAQAVGEDARLATAGNFNNDIGLPLTLFRLNAQHKLAVLELGMNHPGET CHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCH AVLAAIAQPTVAMINNAQREHQEFMVSVEAVAEEHAAVLAALPANGVAVYPRDAANGGEY HHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEECCCCCCCCCCC APVWKAAAGSRRVLDFGMDAGAVTGTVVDTADGQRIEVQAPGQRFVITLPLLGLHNARNA CCHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCHHHH LAATACALAAGVAPEVIAQALGNFAPVKGRLQRKQGTHGGLVIDDTYNANPDSMRAAIDA HHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHH LVTLPAPRWLVLGDMGEVGSKGPAFHEEIGAYARARGIDAVLATGELARHTVDAFGPGAQ HHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHEECCCHHHHHHHHHCCCCHH HFASAEDLIEHGVPAIAPAATVLVKGSRFMRMERIVEALVLKDPVADSPAGSNTQQQH HHCCHHHHHHCCCCCCCCHHHEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]