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The map label for this gene is murE [H]

Identifier: 187930162

GI number: 187930162

Start: 3232177

End: 3233721

Strand: Reverse

Name: murE [H]

Synonym: Rpic_3094

Alternate gene names: 187930162

Gene position: 3233721-3232177 (Counterclockwise)

Preceding gene: 187930163

Following gene: 187930161

Centisome position: 82.02

GC content: 67.57

Gene sequence:

>1545_bases
ATGACAGCCAAGCCCACTTCCGATCGTCCGACGATCGATGCCCGCCTCACGCCGGTGGTGGATTGGTTGCGCGCTCAGGC
ACCCGCGCGTGCCGACCTCACCAGCGACACGCGCAAGCTCAAGACCGGCGATGTGTTCGTGGCCTACGTGCTCGGCAATG
CGCGCCAGCGTGGCGATGGCCGTCCGCATATTCCGCAGGCCATCGAGGCTGGCGCTTCCGCCGTGCTGGCCGAAGCCCAT
GGTTACGCAATGCCCGCCGACGCGCCCGTGCGCATCCTGCCGGTTGACGGCCTGGCTGAACTGGCCGGACCGCTGGCCGC
GCAGTGGTATGGCGTGCCGGGTCCGGAAGCGCTGCGTGTGATTGGTGTGACGGGCACCAATGGCAAGACATCGTGTTCGC
AATGGATTGCGCAGGCGCTGACCAAACACGGCGAGCGTTGCGCAGTGGTGGGTACGCTCGGAACCGGGTTTGTCGACGCG
CTGGCTACCACCGGCTTTACCACGCCGGACGCCATCCAGCTTCAGCGCAGCCTCGCCGATCTGCACCGCGCGGGCGCTCG
CGCCATCGCCATGGAAGTGTCGTCGCATGGCCTGGAGCAAGGCCGGGTGGATGGCACCCGCTTCGACATCGCGGTGTTCA
CCAACCTGACGCAGGATCACCTCGACTATCACGGCACGATGGCCGAGTACGAACTCGCCAAGGCGCGACTGTTCGGCTGG
CAGGGCTTGCGCGCAGCGGTCATCAACCGCGACGACGCGGCCGGTGTGCGCTTGCTGCAGAACGCCCGCGCCGATGTCGA
GACCATCGAATACGGCATCGACGGCGACGCCGCTGCACAGCACGGCGCCAGGCAATGGCTGCGCGCCACCAACGTGCGCG
CACATCGCACCGGCACGACGTTCGACGTAGACGGCAGCTTTGGCCGGGCGACCGTCCATGTGTCCACCGTCGGCCTGTTC
AACGTATCGAATCTCCTGGCTGTGCTTGGCGCACTGCTCGCTGCCGGTGTTTCGTGGCAGGACGCCATCGCACGCCTGGA
AAAACTCCAACCCGTGAGTGGCCGCATGGAGCGGTTTGGAGGCGAAGACGGCCCGTTGGTTGTGGTCGACTACGCTCACA
CGCCCGACGCGTTGGAGCAGACGCTGCGCGCCCTCGCGCCGATCACCGAAGCGCGCGGCGGCAAGCTGTGGGCCGTGTTC
GGCTGCGGCGGCGACCGCGACCCCGGCAAGCGTCCGCAGATGGGCTCCATCGCCGAGCGCCTGGCGCCGCACGTGGTGCT
GACCTCTGACAACCCGCGCAGCGAAGATCCGCAGCACATCCTCGACGAGATCGCCGACGGCATGGACAATCCGCGTGCCG
CCACCCAGATCGAGGACCGCGCGGCTGCCATCCTCCACGCCGTGCGGCATGCCGATGCGCATGACGTGATCGTCGTGGCC
GGCAAGGGGCATGAATCGACGCAGGAGATTGCCGGTCGCAAGCGGCCGTTCTCCGATCAGGAACACGTGCGCCTGGCACT
CGCCGCCCGCGGGGTGAATGCATGA

Upstream 100 bases:

>100_bases
TGGGCGGTACCCTGCGCGCGTTGAACGTGCCGCCGGATTCGCCGGTACGGCAGCTCGTGATGACCCCGGATGTGCCGGAA
GAACCGGTGGGAGGCCTGCA

Downstream 100 bases:

>100_bases
GCGCCGCGACCGTGATGATGCGCGCCACCGACGCCGCTGCCTGGATGACCGGCGCCCATCTGGCCGGCCCCGATGCGGCG
ATCCTGCGCGTGACGACCGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTAKPTSDRPTIDARLTPVVDWLRAQAPARADLTSDTRKLKTGDVFVAYVLGNARQRGDGRPHIPQAIEAGASAVLAEAH
GYAMPADAPVRILPVDGLAELAGPLAAQWYGVPGPEALRVIGVTGTNGKTSCSQWIAQALTKHGERCAVVGTLGTGFVDA
LATTGFTTPDAIQLQRSLADLHRAGARAIAMEVSSHGLEQGRVDGTRFDIAVFTNLTQDHLDYHGTMAEYELAKARLFGW
QGLRAAVINRDDAAGVRLLQNARADVETIEYGIDGDAAAQHGARQWLRATNVRAHRTGTTFDVDGSFGRATVHVSTVGLF
NVSNLLAVLGALLAAGVSWQDAIARLEKLQPVSGRMERFGGEDGPLVVVDYAHTPDALEQTLRALAPITEARGGKLWAVF
GCGGDRDPGKRPQMGSIAERLAPHVVLTSDNPRSEDPQHILDEIADGMDNPRAATQIEDRAAAILHAVRHADAHDVIVVA
GKGHESTQEIAGRKRPFSDQEHVRLALAARGVNA

Sequences:

>Translated_514_residues
MTAKPTSDRPTIDARLTPVVDWLRAQAPARADLTSDTRKLKTGDVFVAYVLGNARQRGDGRPHIPQAIEAGASAVLAEAH
GYAMPADAPVRILPVDGLAELAGPLAAQWYGVPGPEALRVIGVTGTNGKTSCSQWIAQALTKHGERCAVVGTLGTGFVDA
LATTGFTTPDAIQLQRSLADLHRAGARAIAMEVSSHGLEQGRVDGTRFDIAVFTNLTQDHLDYHGTMAEYELAKARLFGW
QGLRAAVINRDDAAGVRLLQNARADVETIEYGIDGDAAAQHGARQWLRATNVRAHRTGTTFDVDGSFGRATVHVSTVGLF
NVSNLLAVLGALLAAGVSWQDAIARLEKLQPVSGRMERFGGEDGPLVVVDYAHTPDALEQTLRALAPITEARGGKLWAVF
GCGGDRDPGKRPQMGSIAERLAPHVVLTSDNPRSEDPQHILDEIADGMDNPRAATQIEDRAAAILHAVRHADAHDVIVVA
GKGHESTQEIAGRKRPFSDQEHVRLALAARGVNA
>Mature_513_residues
TAKPTSDRPTIDARLTPVVDWLRAQAPARADLTSDTRKLKTGDVFVAYVLGNARQRGDGRPHIPQAIEAGASAVLAEAHG
YAMPADAPVRILPVDGLAELAGPLAAQWYGVPGPEALRVIGVTGTNGKTSCSQWIAQALTKHGERCAVVGTLGTGFVDAL
ATTGFTTPDAIQLQRSLADLHRAGARAIAMEVSSHGLEQGRVDGTRFDIAVFTNLTQDHLDYHGTMAEYELAKARLFGWQ
GLRAAVINRDDAAGVRLLQNARADVETIEYGIDGDAAAQHGARQWLRATNVRAHRTGTTFDVDGSFGRATVHVSTVGLFN
VSNLLAVLGALLAAGVSWQDAIARLEKLQPVSGRMERFGGEDGPLVVVDYAHTPDALEQTLRALAPITEARGGKLWAVFG
CGGDRDPGKRPQMGSIAERLAPHVVLTSDNPRSEDPQHILDEIADGMDNPRAATQIEDRAAAILHAVRHADAHDVIVVAG
KGHESTQEIAGRKRPFSDQEHVRLALAARGVNA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=486, Percent_Identity=44.6502057613169, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54707; Mature: 54576

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKPTSDRPTIDARLTPVVDWLRAQAPARADLTSDTRKLKTGDVFVAYVLGNARQRGDG
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHEECCCEEEEEEECCCHHCCCC
RPHIPQAIEAGASAVLAEAHGYAMPADAPVRILPVDGLAELAGPLAAQWYGVPGPEALRV
CCCCHHHHHHCHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHCHHHHHCCCCCCCCCEEE
IGVTGTNGKTSCSQWIAQALTKHGERCAVVGTLGTGFVDALATTGFTTPDAIQLQRSLAD
EEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHH
LHRAGARAIAMEVSSHGLEQGRVDGTRFDIAVFTNLTQDHLDYHGTMAEYELAKARLFGW
HHHCCCCEEEEEHHHCCCCCCCCCCCEEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHCC
QGLRAAVINRDDAAGVRLLQNARADVETIEYGIDGDAAAQHGARQWLRATNVRAHRTGTT
CCCEEEEECCCCHHHHHHHHHCCCCHHHEEECCCCCHHHHHHHHHHHHHCCCEEECCCCE
FDVDGSFGRATVHVSTVGLFNVSNLLAVLGALLAAGVSWQDAIARLEKLQPVSGRMERFG
EECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCC
GEDGPLVVVDYAHTPDALEQTLRALAPITEARGGKLWAVFGCGGDRDPGKRPQMGSIAER
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHH
LAPHVVLTSDNPRSEDPQHILDEIADGMDNPRAATQIEDRAAAILHAVRHADAHDVIVVA
HCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
GKGHESTQEIAGRKRPFSDQEHVRLALAARGVNA
CCCCCHHHHHHCCCCCCCCHHHEEEEEECCCCCC
>Mature Secondary Structure 
TAKPTSDRPTIDARLTPVVDWLRAQAPARADLTSDTRKLKTGDVFVAYVLGNARQRGDG
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHEECCCEEEEEEECCCHHCCCC
RPHIPQAIEAGASAVLAEAHGYAMPADAPVRILPVDGLAELAGPLAAQWYGVPGPEALRV
CCCCHHHHHHCHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHCHHHHHCCCCCCCCCEEE
IGVTGTNGKTSCSQWIAQALTKHGERCAVVGTLGTGFVDALATTGFTTPDAIQLQRSLAD
EEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHH
LHRAGARAIAMEVSSHGLEQGRVDGTRFDIAVFTNLTQDHLDYHGTMAEYELAKARLFGW
HHHCCCCEEEEEHHHCCCCCCCCCCCEEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHCC
QGLRAAVINRDDAAGVRLLQNARADVETIEYGIDGDAAAQHGARQWLRATNVRAHRTGTT
CCCEEEEECCCCHHHHHHHHHCCCCHHHEEECCCCCHHHHHHHHHHHHHCCCEEECCCCE
FDVDGSFGRATVHVSTVGLFNVSNLLAVLGALLAAGVSWQDAIARLEKLQPVSGRMERFG
EECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCC
GEDGPLVVVDYAHTPDALEQTLRALAPITEARGGKLWAVFGCGGDRDPGKRPQMGSIAER
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHH
LAPHVVLTSDNPRSEDPQHILDEIADGMDNPRAATQIEDRAAAILHAVRHADAHDVIVVA
HCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
GKGHESTQEIAGRKRPFSDQEHVRLALAARGVNA
CCCCCHHHHHHCCCCCCCCHHHEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]