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The map label for this gene is ddl

Identifier: 187930155

GI number: 187930155

Start: 3223118

End: 3224113

Strand: Reverse

Name: ddl

Synonym: Rpic_3087

Alternate gene names: 187930155

Gene position: 3224113-3223118 (Counterclockwise)

Preceding gene: 187930156

Following gene: 187930154

Centisome position: 81.78

GC content: 65.36

Gene sequence:

>996_bases
ATGACGACCGGTCCGTTTGTTCCCAATCCGACGATTGATCCGAAGTCCCTCGGCAAAGTGGGCGTGCTCATGGGCGGTCG
TTCGGCCGAGCGCGAAATCTCACTGATGTCCGGCAACGGCGTGCTGGCTGCGCTGCGCGCACGCGGTGTCGATGCACACG
CCTTCGACCCGGGCCTGCAGGCCGTGGCCGATCTGGCCAAGCAAGGGTTCGACCGCGTGGTGATTTCGCTGCACGGCCGC
TTTGGCGAAGACGGCACGATCCAGGGCCTGCTCGAGCAATTCGGTATTCCGTACACCGGCAGCGGCGTACTCGCATCTGC
GCTGGCGATGGACAAGGAAGCCACCAAGCGCCAATGGCAGACCCATGGCCTGCCCACGCCCGATTTCGTGATGCTGCACG
CCGGCGCCGACTGGCAAGCCGTGGCCGACCGCCTGGGCCTGCCGCTGATCGTCAAGCCGGCGCGCGAAGGTTCGTCGATC
GGTCTGACCAAGGTCACGAGCGTCGCCGAGCTGCCCGCCGCCTACGAAAAGGCCGCGCGCCTGGATCGTGATGTGATGGC
CGAGCAGTTCATCGAAGGTGACGAACTGACCTGCCCGATCATCGGCGAAGGGGAGAGCGCTACGGCATTGCCGCTTATCC
GCATCGTCGCGCCGCAGGCCAACTACGACTACCAGAACAAGTACTTCACCGACGACACGCGCTACGAATGCCCGGCACCG
ATTCCCGCTGATGTGGCCGCGCGCGTGCAGGCGCTGGTGGTGCAGGCATATCGCGGGCTGGGCTGCCGTGGCTGGGGCCG
CGCCGACATCATGCTGCGCAAGTCCGATAACGCGCCGTTCCTGCTGGAGATGAACACGTCGCCGGGCATGACCGGCCATT
CGTTGGTGCCGATGGGCGCCCGCGCGGCCGGCATCAGCTATGAAGATTTCGTATTGCAACTGGCGGCGAGTGCGTCGCTG
GAGTTGCACGCGAGCACCGACTGGAAACCCGAGTAA

Upstream 100 bases:

>100_bases
TGGGCGCTGGGTCGATCGGGCAGGTGCCCGGGCAAGTGGTTGCGTTGCAGGCGCAGGCGCGCACTGCAAACGTCGTGGAT
CTGAACGGAGGCGCCGCAGC

Downstream 100 bases:

>100_bases
TCGACACAACGTCAGCGAAACCGGACAACACAACGTTATGTGGCACAACACCCGTCTCCTCAACGCCGTCGCGAGTGCGC
TGTATGCGCTGCTCGCGCTT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTTGPFVPNPTIDPKSLGKVGVLMGGRSAEREISLMSGNGVLAALRARGVDAHAFDPGLQAVADLAKQGFDRVVISLHGR
FGEDGTIQGLLEQFGIPYTGSGVLASALAMDKEATKRQWQTHGLPTPDFVMLHAGADWQAVADRLGLPLIVKPAREGSSI
GLTKVTSVAELPAAYEKAARLDRDVMAEQFIEGDELTCPIIGEGESATALPLIRIVAPQANYDYQNKYFTDDTRYECPAP
IPADVAARVQALVVQAYRGLGCRGWGRADIMLRKSDNAPFLLEMNTSPGMTGHSLVPMGARAAGISYEDFVLQLAASASL
ELHASTDWKPE

Sequences:

>Translated_331_residues
MTTGPFVPNPTIDPKSLGKVGVLMGGRSAEREISLMSGNGVLAALRARGVDAHAFDPGLQAVADLAKQGFDRVVISLHGR
FGEDGTIQGLLEQFGIPYTGSGVLASALAMDKEATKRQWQTHGLPTPDFVMLHAGADWQAVADRLGLPLIVKPAREGSSI
GLTKVTSVAELPAAYEKAARLDRDVMAEQFIEGDELTCPIIGEGESATALPLIRIVAPQANYDYQNKYFTDDTRYECPAP
IPADVAARVQALVVQAYRGLGCRGWGRADIMLRKSDNAPFLLEMNTSPGMTGHSLVPMGARAAGISYEDFVLQLAASASL
ELHASTDWKPE
>Mature_330_residues
TTGPFVPNPTIDPKSLGKVGVLMGGRSAEREISLMSGNGVLAALRARGVDAHAFDPGLQAVADLAKQGFDRVVISLHGRF
GEDGTIQGLLEQFGIPYTGSGVLASALAMDKEATKRQWQTHGLPTPDFVMLHAGADWQAVADRLGLPLIVKPAREGSSIG
LTKVTSVAELPAAYEKAARLDRDVMAEQFIEGDELTCPIIGEGESATALPLIRIVAPQANYDYQNKYFTDDTRYECPAPI
PADVAARVQALVVQAYRGLGCRGWGRADIMLRKSDNAPFLLEMNTSPGMTGHSLVPMGARAAGISYEDFVLQLAASASLE
LHASTDWKPE

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=303, Percent_Identity=50.1650165016502, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1786579, Length=357, Percent_Identity=33.8935574229692, Blast_Score=175, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_RALPJ (B2UCX5)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001900642.1
- ProteinModelPortal:   B2UCX5
- SMR:   B2UCX5
- GeneID:   6286114
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_3087
- HOGENOM:   HBG427092
- OMA:   VPYTGSD
- ProtClustDB:   PRK01372
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 35331; Mature: 35199

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGPFVPNPTIDPKSLGKVGVLMGGRSAEREISLMSGNGVLAALRARGVDAHAFDPGLQ
CCCCCCCCCCCCCHHHCCCEEEEECCCCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHH
AVADLAKQGFDRVVISLHGRFGEDGTIQGLLEQFGIPYTGSGVLASALAMDKEATKRQWQ
HHHHHHHCCHHEEEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
THGLPTPDFVMLHAGADWQAVADRLGLPLIVKPAREGSSIGLTKVTSVAELPAAYEKAAR
HCCCCCCCEEEEECCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LDRDVMAEQFIEGDELTCPIIGEGESATALPLIRIVAPQANYDYQNKYFTDDTRYECPAP
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCCCCC
IPADVAARVQALVVQAYRGLGCRGWGRADIMLRKSDNAPFLLEMNTSPGMTGHSLVPMGA
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCC
RAAGISYEDFVLQLAASASLELHASTDWKPE
HHCCCCHHHHHHHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure 
TTGPFVPNPTIDPKSLGKVGVLMGGRSAEREISLMSGNGVLAALRARGVDAHAFDPGLQ
CCCCCCCCCCCCHHHCCCEEEEECCCCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHH
AVADLAKQGFDRVVISLHGRFGEDGTIQGLLEQFGIPYTGSGVLASALAMDKEATKRQWQ
HHHHHHHCCHHEEEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
THGLPTPDFVMLHAGADWQAVADRLGLPLIVKPAREGSSIGLTKVTSVAELPAAYEKAAR
HCCCCCCCEEEEECCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LDRDVMAEQFIEGDELTCPIIGEGESATALPLIRIVAPQANYDYQNKYFTDDTRYECPAP
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCCCCC
IPADVAARVQALVVQAYRGLGCRGWGRADIMLRKSDNAPFLLEMNTSPGMTGHSLVPMGA
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCC
RAAGISYEDFVLQLAASASLELHASTDWKPE
HHCCCCHHHHHHHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA