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The map label for this gene is proS

Identifier: 187930129

GI number: 187930129

Start: 3195070

End: 3196794

Strand: Reverse

Name: proS

Synonym: Rpic_3061

Alternate gene names: 187930129

Gene position: 3196794-3195070 (Counterclockwise)

Preceding gene: 187930132

Following gene: 187930128

Centisome position: 81.08

GC content: 65.62

Gene sequence:

>1725_bases
ATGAAAGCGTCCCAATTCTTCATTTCCACGCTCAAGGAAGCGCCCGCTGACGCGGAGATCGTCTCGCACAAGTTGATGAT
GCGTGCCGGCATGATCAAGAAGCTTGGCGCGGGCCTCTATACGTACATGCCCGTGGGTCTGCGCGTGATCCGCAAGGTCG
AGCAGATCGTGCGTGAGGAAATGAATGCCGCCGGCGCGGTGGAGGTGCTGATGCCGGTGGTGCAGCCGGGCGAGCTGTGG
CAGGAGACCGGCCGCTGGGACAAGATGGGCGACGAGCTGCTGCGCTTCAAGGATCGCCACGAGCGCGACTTCGTCATGCA
GCCGACGTCGGAGGAGGTGGTGACCGACATCGCCCGCACTGAAATTCGCTCGTACAAGCAGCTGCCCGTCAATTTCTACC
AGATCCAGACCAAGTTCCGCGACGAGCGCCGCCCGCGTTTCGGCATCATGCGCGGCCGCGAATTCACGATGAAGGACGCG
TACTCCTTCGACCGCGACGCCGAGGGCCTGAAGGTGTCGTACCAGAAGATGTACGACGCGTACACGCGCATCTTCCAGCG
CTTTGGCCTGGAATTCCGCGCCGTGGCGGCCGATAACGGCGCGATTGGCGGATCGGGCTCGCACGAATTCCACGTGATTG
CCGACACGGGCGAAGACGCCATCATCTACTGCCCGGATTCGGACTACGCCGCGAACATCGAGGCTGCTGAAGCCGTCGCC
CCGGCCGCACCGCGTGCCGCTGCCACCGAAGCGCTCACCAAGACGCATACGCCCGGCCGCGCCAAGTGCGAGGCCGTGGC
CGAGCAACTGGGTATTCCGCTGCAGCGCACGATCAAATCCATCGTGCTGGCCACCGAAGTCGAGGGCGGCGAGCCCCAGA
TTTGGTTGCTGCTGCTGCGCGGCGACCATGAACTCAACGAGGTCAAGGCATCGAAGGTGCCGGGCCTTGCCGACTTCCGC
TTTGCCACCGAAGGCGAAATCCTGCGTGCGTTCGGCACGCGGCCGGGCTATTTGGGTCCGGTCGGCACGAAGCTGCCCGT
GAAGGTGGTGGCCGATCGCACGGCGGCCGCGATGAGCGATTTCGTGGTCGGCGCCAACGAAGAGGACTACCACTTCACTG
GCGTGAACTGGGGCCGCGATCTGCCCGAGCCGGAGGTCTACGACCTGCGCAATGTGGTGGCAGGCGACGCCTCGCCGGAC
GGCAAGGGCACGCTGGCGATCTGCCGCGGCATCGAAGTCGGTCACGTCTTCATGCTGGGCACGCGCTACTCCGAGGCGAT
GAATGCGACGTTCCTCGATGAGAACGGGAAGACGCAGCCGATGGTGATGGGCTGCTACGGCATCGGCATCACGCGCATCC
TGGGTGCGGCCATCGAGCAGAACTACGATGCGCGCGGGATCATCTGGCCGGCATCGATCGCGCCGTTCCAGGTGGTGATC
TGCCCCGTGGGCTACGACCGGTCCGACGCCGTGCGCGAGGAAGCCGACCGCCTGCACGCCGAGCTGGTCGCCGCCGGCAT
CGACGTGATGTTGGACGACCGCGGCGAGCGCCCCGGCGCGATGTTCGCCGACTGGGAGCTGATCGGCGTGCCGTTCCGCG
TGGTGGTGGGTGACCGCGGCCTGAAGGAAGGCAAGCTGGAATTCCAGGGCCGCCGAGACGAAGCCGCCACCGCCGTGGCG
CCCGCCGACGTGCTGGCTACGCTGAAGGCGCGTCTCGCACAATAA

Upstream 100 bases:

>100_bases
GAATGTGCGCCTGGCGCCTCGTCTCATCTCCTGATTCCGGTGGCTTTTGCCCTTGTTTTCGCGGCTTTGGTTGGTCGCGT
CATCCCTTATTGTCAGTCGC

Downstream 100 bases:

>100_bases
CGCAACAACCGGACCGACCCGCATGCGCCGCTTGTCTTCCGCCCGCCTGATCGTCACTGCGCTGTGCGCGGGGACGTTGC
TCGCGGGCACGCAGCTGGCG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGLYTYMPVGLRVIRKVEQIVREEMNAAGAVEVLMPVVQPGELW
QETGRWDKMGDELLRFKDRHERDFVMQPTSEEVVTDIARTEIRSYKQLPVNFYQIQTKFRDERRPRFGIMRGREFTMKDA
YSFDRDAEGLKVSYQKMYDAYTRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIIYCPDSDYAANIEAAEAVA
PAAPRAAATEALTKTHTPGRAKCEAVAEQLGIPLQRTIKSIVLATEVEGGEPQIWLLLLRGDHELNEVKASKVPGLADFR
FATEGEILRAFGTRPGYLGPVGTKLPVKVVADRTAAAMSDFVVGANEEDYHFTGVNWGRDLPEPEVYDLRNVVAGDASPD
GKGTLAICRGIEVGHVFMLGTRYSEAMNATFLDENGKTQPMVMGCYGIGITRILGAAIEQNYDARGIIWPASIAPFQVVI
CPVGYDRSDAVREEADRLHAELVAAGIDVMLDDRGERPGAMFADWELIGVPFRVVVGDRGLKEGKLEFQGRRDEAATAVA
PADVLATLKARLAQ

Sequences:

>Translated_574_residues
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGLYTYMPVGLRVIRKVEQIVREEMNAAGAVEVLMPVVQPGELW
QETGRWDKMGDELLRFKDRHERDFVMQPTSEEVVTDIARTEIRSYKQLPVNFYQIQTKFRDERRPRFGIMRGREFTMKDA
YSFDRDAEGLKVSYQKMYDAYTRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIIYCPDSDYAANIEAAEAVA
PAAPRAAATEALTKTHTPGRAKCEAVAEQLGIPLQRTIKSIVLATEVEGGEPQIWLLLLRGDHELNEVKASKVPGLADFR
FATEGEILRAFGTRPGYLGPVGTKLPVKVVADRTAAAMSDFVVGANEEDYHFTGVNWGRDLPEPEVYDLRNVVAGDASPD
GKGTLAICRGIEVGHVFMLGTRYSEAMNATFLDENGKTQPMVMGCYGIGITRILGAAIEQNYDARGIIWPASIAPFQVVI
CPVGYDRSDAVREEADRLHAELVAAGIDVMLDDRGERPGAMFADWELIGVPFRVVVGDRGLKEGKLEFQGRRDEAATAVA
PADVLATLKARLAQ
>Mature_574_residues
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGLYTYMPVGLRVIRKVEQIVREEMNAAGAVEVLMPVVQPGELW
QETGRWDKMGDELLRFKDRHERDFVMQPTSEEVVTDIARTEIRSYKQLPVNFYQIQTKFRDERRPRFGIMRGREFTMKDA
YSFDRDAEGLKVSYQKMYDAYTRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIIYCPDSDYAANIEAAEAVA
PAAPRAAATEALTKTHTPGRAKCEAVAEQLGIPLQRTIKSIVLATEVEGGEPQIWLLLLRGDHELNEVKASKVPGLADFR
FATEGEILRAFGTRPGYLGPVGTKLPVKVVADRTAAAMSDFVVGANEEDYHFTGVNWGRDLPEPEVYDLRNVVAGDASPD
GKGTLAICRGIEVGHVFMLGTRYSEAMNATFLDENGKTQPMVMGCYGIGITRILGAAIEQNYDARGIIWPASIAPFQVVI
CPVGYDRSDAVREEADRLHAELVAAGIDVMLDDRGERPGAMFADWELIGVPFRVVVGDRGLKEGKLEFQGRRDEAATAVA
PADVLATLKARLAQ

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=249, Percent_Identity=43.3734939759036, Blast_Score=223, Evalue=3e-58,
Organism=Escherichia coli, GI1786392, Length=570, Percent_Identity=58.7719298245614, Blast_Score=699, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=274, Percent_Identity=36.1313868613139, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI193203271, Length=87, Percent_Identity=36.7816091954023, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=546, Percent_Identity=30.2197802197802, Blast_Score=253, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24656200, Length=257, Percent_Identity=37.3540856031128, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_RALPJ (B2UCU9)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001900616.1
- ProteinModelPortal:   B2UCU9
- SMR:   B2UCU9
- GeneID:   6286858
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_3061
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63261; Mature: 63261

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGLYTYMPVGLRVIRKVEQIVREE
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MNAAGAVEVLMPVVQPGELWQETGRWDKMGDELLRFKDRHERDFVMQPTSEEVVTDIART
HCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EIRSYKQLPVNFYQIQTKFRDERRPRFGIMRGREFTMKDAYSFDRDAEGLKVSYQKMYDA
HHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHH
YTRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIIYCPDSDYAANIEAAEAVA
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHC
PAAPRAAATEALTKTHTPGRAKCEAVAEQLGIPLQRTIKSIVLATEVEGGEPQIWLLLLR
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCCCEEEEEEEE
GDHELNEVKASKVPGLADFRFATEGEILRAFGTRPGYLGPVGTKLPVKVVADRTAAAMSD
CCCHHHHHHHHCCCCCCCEEECCCCHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHH
FVVGANEEDYHFTGVNWGRDLPEPEVYDLRNVVAGDASPDGKGTLAICRGIEVGHVFMLG
HEECCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEE
TRYSEAMNATFLDENGKTQPMVMGCYGIGITRILGAAIEQNYDARGIIWPASIAPFQVVI
CHHHHHCCCEEECCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEE
CPVGYDRSDAVREEADRLHAELVAAGIDVMLDDRGERPGAMFADWELIGVPFRVVVGDRG
EECCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEECEEEECCCEEEEECCCC
LKEGKLEFQGRRDEAATAVAPADVLATLKARLAQ
CCCCCEEECCCCCCHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGLYTYMPVGLRVIRKVEQIVREE
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MNAAGAVEVLMPVVQPGELWQETGRWDKMGDELLRFKDRHERDFVMQPTSEEVVTDIART
HCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EIRSYKQLPVNFYQIQTKFRDERRPRFGIMRGREFTMKDAYSFDRDAEGLKVSYQKMYDA
HHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHH
YTRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIIYCPDSDYAANIEAAEAVA
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHC
PAAPRAAATEALTKTHTPGRAKCEAVAEQLGIPLQRTIKSIVLATEVEGGEPQIWLLLLR
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCCCEEEEEEEE
GDHELNEVKASKVPGLADFRFATEGEILRAFGTRPGYLGPVGTKLPVKVVADRTAAAMSD
CCCHHHHHHHHCCCCCCCEEECCCCHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHH
FVVGANEEDYHFTGVNWGRDLPEPEVYDLRNVVAGDASPDGKGTLAICRGIEVGHVFMLG
HEECCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEE
TRYSEAMNATFLDENGKTQPMVMGCYGIGITRILGAAIEQNYDARGIIWPASIAPFQVVI
CHHHHHCCCEEECCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEE
CPVGYDRSDAVREEADRLHAELVAAGIDVMLDDRGERPGAMFADWELIGVPFRVVVGDRG
EECCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEECEEEECCCEEEEECCCC
LKEGKLEFQGRRDEAATAVAPADVLATLKARLAQ
CCCCCEEECCCCCCHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA