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The map label for this gene is proB

Identifier: 187930132

GI number: 187930132

Start: 3198450

End: 3199574

Strand: Reverse

Name: proB

Synonym: Rpic_3064

Alternate gene names: 187930132

Gene position: 3199574-3198450 (Counterclockwise)

Preceding gene: 187930133

Following gene: 187930129

Centisome position: 81.15

GC content: 66.67

Gene sequence:

>1125_bases
ATGGCCCTGCATTCGCTGATTGCCGATGCGCGCCGCCTTGTCGTCAAGGTCGGTTCCAGCCTGGTTACCAACGACGGCCG
TGGCCTCGATCAGGCCGCCATTGCCCGCTGGGCCGCGCAGATCGCGGCGTTACGGGCGGCGGGCAAGGAAGTCGTGCTGG
TCAGCTCGGGCGCCATCGCCGAGGGCATGCAGCGCTTGGGCTGGGCCAAGCGCCCGAAGGAAATCCACGAGCTGCAGGCC
GCCGCCGCCGTCGGGCAGATGGGGCTGGCGCAGGTGTACGAATCCGAGTTCGCGCGGCACAGCATCCGCACGGCCCAGGT
GCTGCTCACGCATGGCGACCTGGCCGATCGCGAGCGCTATCTCAACGCACGCTCCACGCTGCTCACGCTGCTGAGCCTCG
GTGTCGTGCCGATCATCAACGAGAACGACACCGTCGTCACCGATGAAATCAAGTTCGGCGACAACGACACGCTTGGCGCG
CTTGTCACCAACCTGATCGAAGGCGATGCGCTGATCATCCTGACGGACCAACGCGGCCTGTACACCGCCGACCCGCGCAA
GGACCCGGGCGCCCGCTTTGTCGATGAGGCGCAGGCCGGTACGCCGGACCTGGAGCAGATGGCCGGCGGCGCCGGTTCGA
GCATCGGCAAGGGCGGCATGCTGACGAAGATTCTGGCGGCCAAGCGGGCGGCCAAATCGGGCGCGCACACCATCATTGCT
TCGGGTCGCGAGGCTGATGTGCTTGCGCGACTGGCGAGCGGTGAGGCCATTGGCACGCAACTTCGCGCGCCGACCGGGCG
GATGGCGGCACGCAAGCAGTGGATGATTGACCATCTGCAGCTGCGCGGTCGCGTGGTGCTGGATGCCGGTGCGGTCGAGA
AACTCACTGCCGGCGGCAAGTCGTTGCTGCCGATCGGCGTGACCGAAGTGCAGGGCGAGTTTGCGCGCGGCGAGGTGATT
TCGTGTGTGGATGCGGCGGGGCTGGAAGTTGCCCGCGGGCTGACGAATTATTCATCGGCAGAGGCTCGGCTGATCGCGCG
CAAGGCATCTTCCGAGATCGAGGCGGTGCTGGGCTACGTCAGCGCTGCGGAACTGGTGCACCGGGATAACCTAGTGCTGC
TCTGA

Upstream 100 bases:

>100_bases
CGCAGCCGCACCCGACCAGCGTCTGGACCCGACACTGCACAACGTCGACCACGACGATCAAGCCTAAACACAACAAACAT
CCTTCGGAGACGCGCCCACC

Downstream 100 bases:

>100_bases
TCCAGCCACTCGGCTGAGCATCGATAGAAAAGGCCAACCTTGCGAGGTTGGCCTTTGTGCTTGAGCGCACGCGGTATGCC
GGTGTCTGCCCCTCAGGGCC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MALHSLIADARRLVVKVGSSLVTNDGRGLDQAAIARWAAQIAALRAAGKEVVLVSSGAIAEGMQRLGWAKRPKEIHELQA
AAAVGQMGLAQVYESEFARHSIRTAQVLLTHGDLADRERYLNARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGA
LVTNLIEGDALIILTDQRGLYTADPRKDPGARFVDEAQAGTPDLEQMAGGAGSSIGKGGMLTKILAAKRAAKSGAHTIIA
SGREADVLARLASGEAIGTQLRAPTGRMAARKQWMIDHLQLRGRVVLDAGAVEKLTAGGKSLLPIGVTEVQGEFARGEVI
SCVDAAGLEVARGLTNYSSAEARLIARKASSEIEAVLGYVSAAELVHRDNLVLL

Sequences:

>Translated_374_residues
MALHSLIADARRLVVKVGSSLVTNDGRGLDQAAIARWAAQIAALRAAGKEVVLVSSGAIAEGMQRLGWAKRPKEIHELQA
AAAVGQMGLAQVYESEFARHSIRTAQVLLTHGDLADRERYLNARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGA
LVTNLIEGDALIILTDQRGLYTADPRKDPGARFVDEAQAGTPDLEQMAGGAGSSIGKGGMLTKILAAKRAAKSGAHTIIA
SGREADVLARLASGEAIGTQLRAPTGRMAARKQWMIDHLQLRGRVVLDAGAVEKLTAGGKSLLPIGVTEVQGEFARGEVI
SCVDAAGLEVARGLTNYSSAEARLIARKASSEIEAVLGYVSAAELVHRDNLVLL
>Mature_373_residues
ALHSLIADARRLVVKVGSSLVTNDGRGLDQAAIARWAAQIAALRAAGKEVVLVSSGAIAEGMQRLGWAKRPKEIHELQAA
AAVGQMGLAQVYESEFARHSIRTAQVLLTHGDLADRERYLNARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGAL
VTNLIEGDALIILTDQRGLYTADPRKDPGARFVDEAQAGTPDLEQMAGGAGSSIGKGGMLTKILAAKRAAKSGAHTIIAS
GREADVLARLASGEAIGTQLRAPTGRMAARKQWMIDHLQLRGRVVLDAGAVEKLTAGGKSLLPIGVTEVQGEFARGEVIS
CVDAAGLEVARGLTNYSSAEARLIARKASSEIEAVLGYVSAAELVHRDNLVLL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=292, Percent_Identity=34.2465753424658, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI21361368, Length=294, Percent_Identity=34.0136054421769, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.1693989071038, Blast_Score=281, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17569969, Length=275, Percent_Identity=34.9090909090909, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17569967, Length=275, Percent_Identity=34.9090909090909, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=35.1807228915663, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321822, Length=425, Percent_Identity=31.0588235294118, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI21357643, Length=282, Percent_Identity=34.7517730496454, Blast_Score=142, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_RALPJ (B2UCV2)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001900619.1
- ProteinModelPortal:   B2UCV2
- SMR:   B2UCV2
- GeneID:   6289009
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_3064
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39357; Mature: 39225

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHSLIADARRLVVKVGSSLVTNDGRGLDQAAIARWAAQIAALRAAGKEVVLVSSGAIA
CCHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHH
EGMQRLGWAKRPKEIHELQAAAAVGQMGLAQVYESEFARHSIRTAQVLLTHGDLADRERY
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHH
LNARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGALVTNLIEGDALIILTDQRGL
HHHHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCC
YTADPRKDPGARFVDEAQAGTPDLEQMAGGAGSSIGKGGMLTKILAAKRAAKSGAHTIIA
EECCCCCCCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
SGREADVLARLASGEAIGTQLRAPTGRMAARKQWMIDHLQLRGRVVLDAGAVEKLTAGGK
CCCHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHCCCC
SLLPIGVTEVQGEFARGEVISCVDAAGLEVARGLTNYSSAEARLIARKASSEIEAVLGYV
CCCCCCHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
SAAELVHRDNLVLL
HHHHHHHCCCEEEC
>Mature Secondary Structure 
ALHSLIADARRLVVKVGSSLVTNDGRGLDQAAIARWAAQIAALRAAGKEVVLVSSGAIA
CHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHH
EGMQRLGWAKRPKEIHELQAAAAVGQMGLAQVYESEFARHSIRTAQVLLTHGDLADRERY
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHH
LNARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGALVTNLIEGDALIILTDQRGL
HHHHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCC
YTADPRKDPGARFVDEAQAGTPDLEQMAGGAGSSIGKGGMLTKILAAKRAAKSGAHTIIA
EECCCCCCCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
SGREADVLARLASGEAIGTQLRAPTGRMAARKQWMIDHLQLRGRVVLDAGAVEKLTAGGK
CCCHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHCCCC
SLLPIGVTEVQGEFARGEVISCVDAAGLEVARGLTNYSSAEARLIARKASSEIEAVLGYV
CCCCCCHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
SAAELVHRDNLVLL
HHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA