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The map label for this gene is pilR [H]

Identifier: 187930120

GI number: 187930120

Start: 3185553

End: 3187178

Strand: Reverse

Name: pilR [H]

Synonym: Rpic_3052

Alternate gene names: 187930120

Gene position: 3187178-3185553 (Counterclockwise)

Preceding gene: 187930121

Following gene: 187930119

Centisome position: 80.84

GC content: 68.45

Gene sequence:

>1626_bases
ATGTCCAAAGCCGCCATCGTTCGCGAACCCATTCTCGTCGTCGATGACGAAGCCGACCTGCGCGAGCTGCTGGAGATTTC
CCTGCGCCGCATGGGGCACGACGTCGTGCTCGCCAGCGGGCTGGCCGAGGCGCGCGAGGCGCTCTCTCGCCAGCGCTTCG
CACTTGTGCTGACCGACATGCGCCTGGGCGATGGCCTGGGCATCGAACTCGTGCGCCAGCTTTCTGCCACGGCCGACCGC
ACGCCGGTGGCCGTCATCACCGCCTATGGCAGCGCCGAGAACGCGGTGGAAGCGCTCAAGGCCGGCGCGTTCGACTACAT
CGCCAAGCCGCTCTCGCTTGATCAGTTGCGCAGCCTCGTGCTCAACGCGCTGGGCCGCCAGCAGCGCGATCCCGACCCGG
GCAACGCCGATCTGGCCGAGCGCACCAACGACCTGCTGCCCGGGCATTCCGCTGCGATGCAGGAAGTGCGCCGCTCGCTG
ATGCGCCTGGCACGCAGCATGGCGCCGGTGGTGATCAGCGGCGAATCCGGCAGCGGCAAGGAGCGTGCGGCGCGCGCCGT
GCATGCGCTGTCTGCGCGTGCATCGCGGCCGTTCGTGGCGGTCAACTGCGGCGCGATTCCCGAGAACCTGATGGAGGCCG
AGTTTTTCGGCTACGTGAAGGGCGCTTTTACCGGCGCCGACACTGATCGCCAGGGCTTCTTCCAGGCCGCCAACGGCGGC
ACGCTCATGCTCGATGAAGTGGCCGACCTGCCGCTCACCATGCAGGTGAAACTGCTGCGTGCGTTGCAGGAGCGTCGCGT
ACGCAAGATCGGCGAAAGCCGTGAAGACCCCGTCGATGTGCGCGTCGTGTGCGCGAGCCACCAGAATCTGGCCCGTCTGG
TGGCCGCTGGCCGTTTCCGCGAAGACTTGTTCTACCGCCTGAACGTGCTGGAACTGCGCATGCCCACGCTGCGCGAGCGT
GCCGAAGACGTGCCCGTGCTGGCCGGCGTGCTGCTCGAACAGCTCGCCAGCCGCTACGGCGATCCGCGCCCCAAGCGCCT
GACGCGACCGGCGCTGCAGCAACTGTGCGCGTATCCGTTCCCGGGCAACGTGCGCGAGCTGGAGAACCTGCTGGAGCGCG
CTTACGCCTTTGCGGAAGGCGAGTCGATCGACGTGGACGACCTTGGCGCGCTGGGAGCCGAGATCGAACGCTCGCCGCTG
TTCCACCGCACGCGCGAGGCGCAGGCTGCCGCAGCGGCCCATCACCCGATGTCGCCGGCGCCGATGACGAGTTGGCCTGA
TGCCGTCTACATGCCGGCGCCGGTGCCAAGCCCCTTGGGGATGCCGCAACCGGTCGCACAGCCCGAGCCGGCACCCGTTG
CGCAGCCGGCGGAGCCGGCCCTGCCGAGCGTTTCGCTGCCGATCGATCTGCCGGCCTACCTGGAGTCGGTCGAGCGCAAC
GTGATCCTGGCCGCGCTGGACCAGACCGGCTTCAACCGTACGGCGGCCGCCAAGCTGCTTGGGCTGTCATTCCGCCAGTT
GCGCTATCGCATGCAGCAGCTTGGCATTCGTGATCCGCGGGACGTTGAAGCGTCGCCGGGCAGCGTCGGTGAACCGGCCG
GAAATGGGCTGAACGGCGATGCCTGA

Upstream 100 bases:

>100_bases
GCGACGGGCCTTCGTCATCACCCTGATGTTTGACCAACCTGCCCTGGCCGTAGCGGAATGATCCGCCGGCTGCGAATTTC
CGCCGATCTTCTGCTGATTC

Downstream 100 bases:

>100_bases
ACGGTTGCACGTCGGCGCTGACGGATGGGTGGATGGCGCGCGCCGCCATCCCTCGCCGAATATCGACGCACGGCCCGAAG
GCATGCCCATCGACCTGATC

Product: Fis family two component sigma-54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSKAAIVREPILVVDDEADLRELLEISLRRMGHDVVLASGLAEAREALSRQRFALVLTDMRLGDGLGIELVRQLSATADR
TPVAVITAYGSAENAVEALKAGAFDYIAKPLSLDQLRSLVLNALGRQQRDPDPGNADLAERTNDLLPGHSAAMQEVRRSL
MRLARSMAPVVISGESGSGKERAARAVHALSARASRPFVAVNCGAIPENLMEAEFFGYVKGAFTGADTDRQGFFQAANGG
TLMLDEVADLPLTMQVKLLRALQERRVRKIGESREDPVDVRVVCASHQNLARLVAAGRFREDLFYRLNVLELRMPTLRER
AEDVPVLAGVLLEQLASRYGDPRPKRLTRPALQQLCAYPFPGNVRELENLLERAYAFAEGESIDVDDLGALGAEIERSPL
FHRTREAQAAAAAHHPMSPAPMTSWPDAVYMPAPVPSPLGMPQPVAQPEPAPVAQPAEPALPSVSLPIDLPAYLESVERN
VILAALDQTGFNRTAAAKLLGLSFRQLRYRMQQLGIRDPRDVEASPGSVGEPAGNGLNGDA

Sequences:

>Translated_541_residues
MSKAAIVREPILVVDDEADLRELLEISLRRMGHDVVLASGLAEAREALSRQRFALVLTDMRLGDGLGIELVRQLSATADR
TPVAVITAYGSAENAVEALKAGAFDYIAKPLSLDQLRSLVLNALGRQQRDPDPGNADLAERTNDLLPGHSAAMQEVRRSL
MRLARSMAPVVISGESGSGKERAARAVHALSARASRPFVAVNCGAIPENLMEAEFFGYVKGAFTGADTDRQGFFQAANGG
TLMLDEVADLPLTMQVKLLRALQERRVRKIGESREDPVDVRVVCASHQNLARLVAAGRFREDLFYRLNVLELRMPTLRER
AEDVPVLAGVLLEQLASRYGDPRPKRLTRPALQQLCAYPFPGNVRELENLLERAYAFAEGESIDVDDLGALGAEIERSPL
FHRTREAQAAAAAHHPMSPAPMTSWPDAVYMPAPVPSPLGMPQPVAQPEPAPVAQPAEPALPSVSLPIDLPAYLESVERN
VILAALDQTGFNRTAAAKLLGLSFRQLRYRMQQLGIRDPRDVEASPGSVGEPAGNGLNGDA
>Mature_540_residues
SKAAIVREPILVVDDEADLRELLEISLRRMGHDVVLASGLAEAREALSRQRFALVLTDMRLGDGLGIELVRQLSATADRT
PVAVITAYGSAENAVEALKAGAFDYIAKPLSLDQLRSLVLNALGRQQRDPDPGNADLAERTNDLLPGHSAAMQEVRRSLM
RLARSMAPVVISGESGSGKERAARAVHALSARASRPFVAVNCGAIPENLMEAEFFGYVKGAFTGADTDRQGFFQAANGGT
LMLDEVADLPLTMQVKLLRALQERRVRKIGESREDPVDVRVVCASHQNLARLVAAGRFREDLFYRLNVLELRMPTLRERA
EDVPVLAGVLLEQLASRYGDPRPKRLTRPALQQLCAYPFPGNVRELENLLERAYAFAEGESIDVDDLGALGAEIERSPLF
HRTREAQAAAAAHHPMSPAPMTSWPDAVYMPAPVPSPLGMPQPVAQPEPAPVAQPAEPALPSVSLPIDLPAYLESVERNV
ILAALDQTGFNRTAAAKLLGLSFRQLRYRMQQLGIRDPRDVEASPGSVGEPAGNGLNGDA

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=501, Percent_Identity=39.9201596806387, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1788550, Length=520, Percent_Identity=37.5, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1790299, Length=509, Percent_Identity=36.3457760314342, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI1788905, Length=368, Percent_Identity=41.5760869565217, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1789233, Length=241, Percent_Identity=48.1327800829876, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082117, Length=236, Percent_Identity=47.8813559322034, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1786524, Length=247, Percent_Identity=47.3684210526316, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI87082152, Length=247, Percent_Identity=46.5587044534413, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1789087, Length=255, Percent_Identity=42.7450980392157, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1787583, Length=239, Percent_Identity=41.8410041841004, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87081872, Length=371, Percent_Identity=32.6145552560647, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789828, Length=229, Percent_Identity=35.8078602620087, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081858, Length=225, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1786599, Length=109, Percent_Identity=39.4495412844037, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 58632; Mature: 58501

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAAIVREPILVVDDEADLRELLEISLRRMGHDVVLASGLAEAREALSRQRFALVLTDM
CCCCHHECCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEHH
RLGDGLGIELVRQLSATADRTPVAVITAYGSAENAVEALKAGAFDYIAKPLSLDQLRSLV
HCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHH
LNALGRQQRDPDPGNADLAERTNDLLPGHSAAMQEVRRSLMRLARSMAPVVISGESGSGK
HHHHHCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCH
ERAARAVHALSARASRPFVAVNCGAIPENLMEAEFFGYVKGAFTGADTDRQGFFQAANGG
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
TLMLDEVADLPLTMQVKLLRALQERRVRKIGESREDPVDVRVVCASHQNLARLVAAGRFR
EEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
EDLFYRLNVLELRMPTLRERAEDVPVLAGVLLEQLASRYGDPRPKRLTRPALQQLCAYPF
HHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHCCCC
PGNVRELENLLERAYAFAEGESIDVDDLGALGAEIERSPLFHRTREAQAAAAAHHPMSPA
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCCCC
PMTSWPDAVYMPAPVPSPLGMPQPVAQPEPAPVAQPAEPALPSVSLPIDLPAYLESVERN
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
VILAALDQTGFNRTAAAKLLGLSFRQLRYRMQQLGIRDPRDVEASPGSVGEPAGNGLNGD
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
A
C
>Mature Secondary Structure 
SKAAIVREPILVVDDEADLRELLEISLRRMGHDVVLASGLAEAREALSRQRFALVLTDM
CCCHHECCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEHH
RLGDGLGIELVRQLSATADRTPVAVITAYGSAENAVEALKAGAFDYIAKPLSLDQLRSLV
HCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHH
LNALGRQQRDPDPGNADLAERTNDLLPGHSAAMQEVRRSLMRLARSMAPVVISGESGSGK
HHHHHCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCH
ERAARAVHALSARASRPFVAVNCGAIPENLMEAEFFGYVKGAFTGADTDRQGFFQAANGG
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
TLMLDEVADLPLTMQVKLLRALQERRVRKIGESREDPVDVRVVCASHQNLARLVAAGRFR
EEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
EDLFYRLNVLELRMPTLRERAEDVPVLAGVLLEQLASRYGDPRPKRLTRPALQQLCAYPF
HHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHCCCC
PGNVRELENLLERAYAFAEGESIDVDDLGALGAEIERSPLFHRTREAQAAAAAHHPMSPA
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCCCC
PMTSWPDAVYMPAPVPSPLGMPQPVAQPEPAPVAQPAEPALPSVSLPIDLPAYLESVERN
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
VILAALDQTGFNRTAAAKLLGLSFRQLRYRMQQLGIRDPRDVEASPGSVGEPAGNGLNGD
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]