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The map label for this gene is pilS [H]

Identifier: 187930121

GI number: 187930121

Start: 3187218

End: 3189257

Strand: Reverse

Name: pilS [H]

Synonym: Rpic_3053

Alternate gene names: 187930121

Gene position: 3189257-3187218 (Counterclockwise)

Preceding gene: 187930122

Following gene: 187930120

Centisome position: 80.89

GC content: 67.55

Gene sequence:

>2040_bases
ATGACGCCCGACGCGTCGGGTGACGCGCGGGCAACACCGTCCACCGCCCGCAGGCTGCTCTCGCGCCTGAACGCCTGGCG
CGCACTCCGATCAGTCTGGCTGGAGCCTGATCCGCCTGAGTTCCAGTGGCGCCTGCTGCGCTATTTCGCCTTCAGCCGCG
CCGCCGTGGCGCTGGTGCTGTTGCTGTTCGTGATGGTGCCGCGCGAGCACAACGAAGCAGCCGGCCTGCCCAATAGCGAA
GCGCTGCTCAGCCTGACGCTGCCGTATCTGGCGATGGCCCTGCTCATCCTTGCCGCAGCCGGCTGGTGGCGCACGCGATT
CCAATTCCGCGTCCGGCTGGATGTGGTGCTCGATCTGCTCTTTCTGGGGCTGGCATACGCCACGCTCTCGCGGCTGTCGG
CGAGCGTGGCAATGGTACTGCTGGTGCCGGTGCTGGCCGCCGGTGCGCTCACGAGCCTGCTCTTCGCGCTGTTCACGGCG
GCAGTGGCGTCGATCGTGGTGCTGACCGATCCGTTCCTGCAGATGATGCGCGAGGGCGTGATTGCGCCGGGGTTGGCATC
CGCCGGGCTGTACGGCCTGGTCTACATGATGGCCGCGTCGATGATGTACGGCCTGTCGCACCGTCAGGTTGCGCAGGAAC
GGTTGACGATGGCCCGCGAGCGGGAGTTGCGCCTGCAACAGCTCGTCAACCGCCTGATGGTCTACGACATGCAGGACGGC
GTGATGCTGGTGCGTGCCGACGGCCGCGTCGTTGCCGCCAACCCGGCCGCAGCCATGCTGCTGGGCGTGCCGCAGAGCGC
GTTTGTCGGCAGCGGTGCGATGTTGTTCGACCTGAAGGACGTGCCGCACCTGCGTCCGCTGCTCGAAACGCTGCGTCAGT
GGCTGCGCCGCAAGAGCCGCCCCGCCGATGGCACCGGTGACGACGATGCACCGCGCATTCTCGACCTGCTGCCCATCGCC
TCGGGCGGCCGGGGTGGCCGCGCCATGTTGAGCGCCCGGCTGCGCTTGCGCTTCATCCTGCCGAGCCTGGCCAACCTGCG
CAGCGTCTATATGGATAGCCTTGTCAGCTCGATCGGCCTGGGCCTGCCGGGCGAGGGCGGCGAGATGCGTCCTCGCATTC
CATCGGCCGAGGCGATCACGCAGGGCTGGTCTGTGGATGACGAAGCGTTTCTGCGCCACGAGTTGCATGACACCGTGCTG
GTCCACGTGGAAAGCTGGGAGCGTGTGGCGGAGCAGGCGCAGCAGGAAAAGCTGGCCTCGATGGGGCGGCTGGTGGCCAG
CGTCGCGCACCAGATCCGCAACCCGTTGGCTGCGATCAGCCAGGCCGCCGAACTGCTGGACGACCCGGGCGACGGCCATG
ATGGCGGTACACATGTCCGCCTGGAAAGCTCGGGCGTGGAGACGCGCCTGCTGCGCATCATCCGCGACAACGTGCGCCGG
CTCGACCAGGTGGTCGCCGACGTGCTGATGCTCTCGCGCCGCCCGCGTGGCGAACGCGTGCGCGTGCAACTGGCGCAGGT
GTTACCGGAAGTGGTCGAGCGCTGGCGTGCCGAAGCCCTGCGCCGCGCAGGCGAAGCGACCGAGATCAACCCGAACCTCG
TGCGCGTGGCGGTCGACCTGGACAAGCCCGTGCTCTTCGACCCGGCGCAGTTGCAGCAGGTGGTGGGCAACATGCTCGAC
AACGCGCTGCGCTATTGCCGCCGTGTGCCAGGTTCGATCCAGCTGGCAGCCTACGCGCTGGACGAGACCCACGCCGAACT
CGTGATCTGGAACGACGGCCCGGAAGTGCCCACCGAGCAGCAGCGCAGCCTGTTCGAGCCGTTCTTCACGAATGACGCCC
AAGGCACTGGCCTGGGCCTCTACATGGCGCGCGAGTTGTGCAGCGCCAACGATGCGCAGATCCGTTATGGCGCCATCGCA
CTGGAATCCTTGCTCGATCGCACCGGAGCGTTGACCATGGAGGCGCGCGACACGTTGCCGCGACGGGCCTTCGTCATCAC
CCTGATGTTTGACCAACCTGCCCTGGCCGTAGCGGAATGA

Upstream 100 bases:

>100_bases
AGCAATGTGCCGTGTGCGGCGTGCACGCGCCGCGCACCGGCATGATCGCGCTGCCGGGCGGCCGCTATCGCTGCCCCGAG
CATGCTGACCGGGGCAACAC

Downstream 100 bases:

>100_bases
TCCGCCGGCTGCGAATTTCCGCCGATCTTCTGCTGATTCATGTCCAAAGCCGCCATCGTTCGCGAACCCATTCTCGTCGT
CGATGACGAAGCCGACCTGC

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MTPDASGDARATPSTARRLLSRLNAWRALRSVWLEPDPPEFQWRLLRYFAFSRAAVALVLLLFVMVPREHNEAAGLPNSE
ALLSLTLPYLAMALLILAAAGWWRTRFQFRVRLDVVLDLLFLGLAYATLSRLSASVAMVLLVPVLAAGALTSLLFALFTA
AVASIVVLTDPFLQMMREGVIAPGLASAGLYGLVYMMAASMMYGLSHRQVAQERLTMARERELRLQQLVNRLMVYDMQDG
VMLVRADGRVVAANPAAAMLLGVPQSAFVGSGAMLFDLKDVPHLRPLLETLRQWLRRKSRPADGTGDDDAPRILDLLPIA
SGGRGGRAMLSARLRLRFILPSLANLRSVYMDSLVSSIGLGLPGEGGEMRPRIPSAEAITQGWSVDDEAFLRHELHDTVL
VHVESWERVAEQAQQEKLASMGRLVASVAHQIRNPLAAISQAAELLDDPGDGHDGGTHVRLESSGVETRLLRIIRDNVRR
LDQVVADVLMLSRRPRGERVRVQLAQVLPEVVERWRAEALRRAGEATEINPNLVRVAVDLDKPVLFDPAQLQQVVGNMLD
NALRYCRRVPGSIQLAAYALDETHAELVIWNDGPEVPTEQQRSLFEPFFTNDAQGTGLGLYMARELCSANDAQIRYGAIA
LESLLDRTGALTMEARDTLPRRAFVITLMFDQPALAVAE

Sequences:

>Translated_679_residues
MTPDASGDARATPSTARRLLSRLNAWRALRSVWLEPDPPEFQWRLLRYFAFSRAAVALVLLLFVMVPREHNEAAGLPNSE
ALLSLTLPYLAMALLILAAAGWWRTRFQFRVRLDVVLDLLFLGLAYATLSRLSASVAMVLLVPVLAAGALTSLLFALFTA
AVASIVVLTDPFLQMMREGVIAPGLASAGLYGLVYMMAASMMYGLSHRQVAQERLTMARERELRLQQLVNRLMVYDMQDG
VMLVRADGRVVAANPAAAMLLGVPQSAFVGSGAMLFDLKDVPHLRPLLETLRQWLRRKSRPADGTGDDDAPRILDLLPIA
SGGRGGRAMLSARLRLRFILPSLANLRSVYMDSLVSSIGLGLPGEGGEMRPRIPSAEAITQGWSVDDEAFLRHELHDTVL
VHVESWERVAEQAQQEKLASMGRLVASVAHQIRNPLAAISQAAELLDDPGDGHDGGTHVRLESSGVETRLLRIIRDNVRR
LDQVVADVLMLSRRPRGERVRVQLAQVLPEVVERWRAEALRRAGEATEINPNLVRVAVDLDKPVLFDPAQLQQVVGNMLD
NALRYCRRVPGSIQLAAYALDETHAELVIWNDGPEVPTEQQRSLFEPFFTNDAQGTGLGLYMARELCSANDAQIRYGAIA
LESLLDRTGALTMEARDTLPRRAFVITLMFDQPALAVAE
>Mature_678_residues
TPDASGDARATPSTARRLLSRLNAWRALRSVWLEPDPPEFQWRLLRYFAFSRAAVALVLLLFVMVPREHNEAAGLPNSEA
LLSLTLPYLAMALLILAAAGWWRTRFQFRVRLDVVLDLLFLGLAYATLSRLSASVAMVLLVPVLAAGALTSLLFALFTAA
VASIVVLTDPFLQMMREGVIAPGLASAGLYGLVYMMAASMMYGLSHRQVAQERLTMARERELRLQQLVNRLMVYDMQDGV
MLVRADGRVVAANPAAAMLLGVPQSAFVGSGAMLFDLKDVPHLRPLLETLRQWLRRKSRPADGTGDDDAPRILDLLPIAS
GGRGGRAMLSARLRLRFILPSLANLRSVYMDSLVSSIGLGLPGEGGEMRPRIPSAEAITQGWSVDDEAFLRHELHDTVLV
HVESWERVAEQAQQEKLASMGRLVASVAHQIRNPLAAISQAAELLDDPGDGHDGGTHVRLESSGVETRLLRIIRDNVRRL
DQVVADVLMLSRRPRGERVRVQLAQVLPEVVERWRAEALRRAGEATEINPNLVRVAVDLDKPVLFDPAQLQQVVGNMLDN
ALRYCRRVPGSIQLAAYALDETHAELVIWNDGPEVPTEQQRSLFEPFFTNDAQGTGLGLYMARELCSANDAQIRYGAIAL
ESLLDRTGALTMEARDTLPRRAFVITLMFDQPALAVAE

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=233, Percent_Identity=26.6094420600858, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=27.3127753303965, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=241, Percent_Identity=29.045643153527, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790300, Length=243, Percent_Identity=30.0411522633745, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 74878; Mature: 74747

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDASGDARATPSTARRLLSRLNAWRALRSVWLEPDPPEFQWRLLRYFAFSRAAVALVL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLFVMVPREHNEAAGLPNSEALLSLTLPYLAMALLILAAAGWWRTRFQFRVRLDVVLDLL
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
FLGLAYATLSRLSASVAMVLLVPVLAAGALTSLLFALFTAAVASIVVLTDPFLQMMREGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IAPGLASAGLYGLVYMMAASMMYGLSHRQVAQERLTMARERELRLQQLVNRLMVYDMQDG
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
VMLVRADGRVVAANPAAAMLLGVPQSAFVGSGAMLFDLKDVPHLRPLLETLRQWLRRKSR
EEEEEECCEEEECCCHHHHHCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCC
PADGTGDDDAPRILDLLPIASGGRGGRAMLSARLRLRFILPSLANLRSVYMDSLVSSIGL
CCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLPGEGGEMRPRIPSAEAITQGWSVDDEAFLRHELHDTVLVHVESWERVAEQAQQEKLAS
CCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHH
MGRLVASVAHQIRNPLAAISQAAELLDDPGDGHDGGTHVRLESSGVETRLLRIIRDNVRR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
LDQVVADVLMLSRRPRGERVRVQLAQVLPEVVERWRAEALRRAGEATEINPNLVRVAVDL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
DKPVLFDPAQLQQVVGNMLDNALRYCRRVPGSIQLAAYALDETHAELVIWNDGPEVPTEQ
CCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCCCCHH
QRSLFEPFFTNDAQGTGLGLYMARELCSANDAQIRYGAIALESLLDRTGALTMEARDTLP
HHHHHCHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEHHHHCCC
RRAFVITLMFDQPALAVAE
CEEEEEEEEECCCCHHCCC
>Mature Secondary Structure 
TPDASGDARATPSTARRLLSRLNAWRALRSVWLEPDPPEFQWRLLRYFAFSRAAVALVL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLFVMVPREHNEAAGLPNSEALLSLTLPYLAMALLILAAAGWWRTRFQFRVRLDVVLDLL
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
FLGLAYATLSRLSASVAMVLLVPVLAAGALTSLLFALFTAAVASIVVLTDPFLQMMREGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IAPGLASAGLYGLVYMMAASMMYGLSHRQVAQERLTMARERELRLQQLVNRLMVYDMQDG
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
VMLVRADGRVVAANPAAAMLLGVPQSAFVGSGAMLFDLKDVPHLRPLLETLRQWLRRKSR
EEEEEECCEEEECCCHHHHHCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCC
PADGTGDDDAPRILDLLPIASGGRGGRAMLSARLRLRFILPSLANLRSVYMDSLVSSIGL
CCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLPGEGGEMRPRIPSAEAITQGWSVDDEAFLRHELHDTVLVHVESWERVAEQAQQEKLAS
CCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHH
MGRLVASVAHQIRNPLAAISQAAELLDDPGDGHDGGTHVRLESSGVETRLLRIIRDNVRR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
LDQVVADVLMLSRRPRGERVRVQLAQVLPEVVERWRAEALRRAGEATEINPNLVRVAVDL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
DKPVLFDPAQLQQVVGNMLDNALRYCRRVPGSIQLAAYALDETHAELVIWNDGPEVPTEQ
CCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCCCCHH
QRSLFEPFFTNDAQGTGLGLYMARELCSANDAQIRYGAIALESLLDRTGALTMEARDTLP
HHHHHCHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEHHHHCCC
RRAFVITLMFDQPALAVAE
CEEEEEEEEECCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]