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The map label for this gene is proA

Identifier: 187930048

GI number: 187930048

Start: 3104941

End: 3106221

Strand: Reverse

Name: proA

Synonym: Rpic_2980

Alternate gene names: 187930048

Gene position: 3106221-3104941 (Counterclockwise)

Preceding gene: 187930049

Following gene: 187930047

Centisome position: 78.79

GC content: 64.72

Gene sequence:

>1281_bases
ATGAAGCAGCTCGACGTCAACGAATACATGGCCCTGGTCGGCCGTCAGGCGCGCACTGCCTCGCGCGGCATGGCACGCGC
CGGCACTGCGCAAAAGAACCGCGCGCTGTTGCATATTGCGGCCGCTGTCCGTCGCGATGCCGCCAAGCTCAAAGAGATCA
ACGCCCGCGACGTCGAGCGTGCCCGCGCCAACGGCCAGGATGCTGCCTTTATCGACCGCCTCACGCTGACCGACCGCGCC
ATCGATACCATGGCCGCGGGCCTGGAGCAGATCGCTGCGCTGCCCGACCCGATCGGCGAGATCAGCAATATGAAGTTCCG
GCCCACCGGCATCCAGGTCGGCCAGATGCGCGTGCCGCTTGGCGTGATCGGCATCATCTATGAGTCGCGGCCGAACGTGA
CGGTGGATGCCGCGGCGCTGTGCATCAAGTCGGGCAACGCGACCATTCTGCGAGGCGGTTCCGAAGCGATCGAATCGAAC
GGCGCGCTGGCGGCGCTCATCGAGGAAGGCTTGGCAGACGCAGGCCTGCCGGCCAACGCCGTACAAGTGGTGGCTACGAC
GGATCGCGCGGCCGTCGGCAAGCTGATCACCATGACGGAGTACGTCGATGTGATCGTGCCTCGCGGCGGCAAGAGCCTGA
TCGCGCGACTGATCGAAGAAGCACGCGTGCCGATGATCAAGCATCTGGACGGCATCTGCCACGTCTACATCGACGCCGAC
GCGGACATCGAGAAGGCCGTGCGCGTGTGCGACAACGCCAAGACGCAACGCTATGCGCCGTGCAACACGATGGAAACGCT
GCTCGTGTCGCGCGATGTGGCCGCACGCGCGCTGCCGCCGCTGGCACGTATCTACCAGGACAAGGGCGTCGAACTGCGGG
TGTGCCCGGGCACGCGCGCTACGCTCGAAGCTGCAGGCTTCGGCAATCTGAAAGATGCCGTCGAGGCCGACTGGCACACC
GAATATCTTGCGCCGATCCTGTCGATCCGCACCGTTGACGGTCTGGACGCGGCCATCGAGCACATCAATACCTACGGTTC
CGCGCACACCGATTCGATCATCACGGAGAACTATTCGACCGGCATGCGCTTCCTGCGCGAGGTCGATTCGGCCAGCGTGA
TGATCAACGCGTCCACGCGCTTTGCCGATGGATTCGAGTACGGCCTGGGCGCCGAGATCGGCATCTCCAACGACAAGCTG
CATGCGCGTGGGCCGGTCGGGCTGGAGGGACTGACCTCGCTGAAGTACGTCGTGTTCGGGCACGGCGAGATTCGCACCTG
A

Upstream 100 bases:

>100_bases
GCCGGGCAGCCTCCCGGTGCCGGCCTGATCCGCGCCATCGGCGCAAGCCTTCGAATTGTCCTCACACTGAATTGCCGGCA
GCTTGCCGGTCGCGATCATC

Downstream 100 bases:

>100_bases
TATCGCTTGCTGCACATTGAGCGCCGCCACGAGCGGCGCGCTGTTTTTACCGATCGCGCTTCACTCCATTCACGGGACGG
TTCGCATGCTCTGGGTCAAA

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKQLDVNEYMALVGRQARTASRGMARAGTAQKNRALLHIAAAVRRDAAKLKEINARDVERARANGQDAAFIDRLTLTDRA
IDTMAAGLEQIAALPDPIGEISNMKFRPTGIQVGQMRVPLGVIGIIYESRPNVTVDAAALCIKSGNATILRGGSEAIESN
GALAALIEEGLADAGLPANAVQVVATTDRAAVGKLITMTEYVDVIVPRGGKSLIARLIEEARVPMIKHLDGICHVYIDAD
ADIEKAVRVCDNAKTQRYAPCNTMETLLVSRDVAARALPPLARIYQDKGVELRVCPGTRATLEAAGFGNLKDAVEADWHT
EYLAPILSIRTVDGLDAAIEHINTYGSAHTDSIITENYSTGMRFLREVDSASVMINASTRFADGFEYGLGAEIGISNDKL
HARGPVGLEGLTSLKYVVFGHGEIRT

Sequences:

>Translated_426_residues
MKQLDVNEYMALVGRQARTASRGMARAGTAQKNRALLHIAAAVRRDAAKLKEINARDVERARANGQDAAFIDRLTLTDRA
IDTMAAGLEQIAALPDPIGEISNMKFRPTGIQVGQMRVPLGVIGIIYESRPNVTVDAAALCIKSGNATILRGGSEAIESN
GALAALIEEGLADAGLPANAVQVVATTDRAAVGKLITMTEYVDVIVPRGGKSLIARLIEEARVPMIKHLDGICHVYIDAD
ADIEKAVRVCDNAKTQRYAPCNTMETLLVSRDVAARALPPLARIYQDKGVELRVCPGTRATLEAAGFGNLKDAVEADWHT
EYLAPILSIRTVDGLDAAIEHINTYGSAHTDSIITENYSTGMRFLREVDSASVMINASTRFADGFEYGLGAEIGISNDKL
HARGPVGLEGLTSLKYVVFGHGEIRT
>Mature_426_residues
MKQLDVNEYMALVGRQARTASRGMARAGTAQKNRALLHIAAAVRRDAAKLKEINARDVERARANGQDAAFIDRLTLTDRA
IDTMAAGLEQIAALPDPIGEISNMKFRPTGIQVGQMRVPLGVIGIIYESRPNVTVDAAALCIKSGNATILRGGSEAIESN
GALAALIEEGLADAGLPANAVQVVATTDRAAVGKLITMTEYVDVIVPRGGKSLIARLIEEARVPMIKHLDGICHVYIDAD
ADIEKAVRVCDNAKTQRYAPCNTMETLLVSRDVAARALPPLARIYQDKGVELRVCPGTRATLEAAGFGNLKDAVEADWHT
EYLAPILSIRTVDGLDAAIEHINTYGSAHTDSIITENYSTGMRFLREVDSASVMINASTRFADGFEYGLGAEIGISNDKL
HARGPVGLEGLTSLKYVVFGHGEIRT

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=415, Percent_Identity=36.8674698795181, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI21361368, Length=415, Percent_Identity=36.8674698795181, Blast_Score=256, Evalue=2e-68,
Organism=Escherichia coli, GI1786438, Length=413, Percent_Identity=42.1307506053269, Blast_Score=337, Evalue=8e-94,
Organism=Caenorhabditis elegans, GI17569969, Length=416, Percent_Identity=36.5384615384615, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17569967, Length=416, Percent_Identity=36.5384615384615, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6324899, Length=428, Percent_Identity=35.7476635514019, Blast_Score=282, Evalue=8e-77,
Organism=Drosophila melanogaster, GI21357643, Length=413, Percent_Identity=38.7409200968523, Blast_Score=271, Evalue=7e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_RALPJ (B2UC98)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001900535.1
- ProteinModelPortal:   B2UC98
- GeneID:   6289703
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_2980
- HOGENOM:   HBG318080
- OMA:   GICGAME
- ProtClustDB:   PRK00197
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45658; Mature: 45658

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLDVNEYMALVGRQARTASRGMARAGTAQKNRALLHIAAAVRRDAAKLKEINARDVER
CCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
ARANGQDAAFIDRLTLTDRAIDTMAAGLEQIAALPDPIGEISNMKFRPTGIQVGQMRVPL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEECCCCEEECEEECCE
GVIGIIYESRPNVTVDAAALCIKSGNATILRGGSEAIESNGALAALIEEGLADAGLPANA
EEEEEEEECCCCEEEEEEEEEEECCCEEEEECCHHHHHCCCCCHHHHHHHHHCCCCCCCC
VQVVATTDRAAVGKLITMTEYVDVIVPRGGKSLIARLIEEARVPMIKHLDGICHVYIDAD
EEEEEECCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCHHHHCCCEEEEEECCC
ADIEKAVRVCDNAKTQRYAPCNTMETLLVSRDVAARALPPLARIYQDKGVELRVCPGTRA
CCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCC
TLEAAGFGNLKDAVEADWHTEYLAPILSIRTVDGLDAAIEHINTYGSAHTDSIITENYST
EEEECCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHCCHHH
GMRFLREVDSASVMINASTRFADGFEYGLGAEIGISNDKLHARGPVGLEGLTSLKYVVFG
HHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEECCCCCCEECCCCCCCCHHHHEEEEEEE
HGEIRT
CCCCCC
>Mature Secondary Structure
MKQLDVNEYMALVGRQARTASRGMARAGTAQKNRALLHIAAAVRRDAAKLKEINARDVER
CCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
ARANGQDAAFIDRLTLTDRAIDTMAAGLEQIAALPDPIGEISNMKFRPTGIQVGQMRVPL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEECCCCEEECEEECCE
GVIGIIYESRPNVTVDAAALCIKSGNATILRGGSEAIESNGALAALIEEGLADAGLPANA
EEEEEEEECCCCEEEEEEEEEEECCCEEEEECCHHHHHCCCCCHHHHHHHHHCCCCCCCC
VQVVATTDRAAVGKLITMTEYVDVIVPRGGKSLIARLIEEARVPMIKHLDGICHVYIDAD
EEEEEECCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCHHHHCCCEEEEEECCC
ADIEKAVRVCDNAKTQRYAPCNTMETLLVSRDVAARALPPLARIYQDKGVELRVCPGTRA
CCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCC
TLEAAGFGNLKDAVEADWHTEYLAPILSIRTVDGLDAAIEHINTYGSAHTDSIITENYST
EEEECCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHCCHHH
GMRFLREVDSASVMINASTRFADGFEYGLGAEIGISNDKLHARGPVGLEGLTSLKYVVFG
HHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEECCCCCCEECCCCCCCCHHHHEEEEEEE
HGEIRT
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA