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The map label for this gene is rlmL [H]

Identifier: 187930046

GI number: 187930046

Start: 3102998

End: 3104416

Strand: Reverse

Name: rlmL [H]

Synonym: Rpic_2978

Alternate gene names: 187930046

Gene position: 3104416-3102998 (Counterclockwise)

Preceding gene: 187930047

Following gene: 187930036

Centisome position: 78.74

GC content: 68.29

Gene sequence:

>1419_bases
ATGGCAACTGCTTACTTCGCGCCATGCCCCCGTGGGCTGGAACAGGCGCTGGCCGATGAACTGGCCGAAATCGCCATCCG
CCCCGAGGTGGATGAGCTGGCCGCGTTCGAGGTGGGCCGCACCGTGCCGGGCGGCGTGCACTTCTTCGGCACGCAGGGCG
CGGCGTATGCCGTCAACCTGCACAGCCGCATTGCCAGCCGTGTGCTGATGCGCCTGGGCTCGCGCGGGTATCGCTCGGAA
GACGACATCTACGCCTTGGCCGCTGCGCAGCGCTGGGAAGACTATTTCACGCCCGACGAAATGATCCGCGTGGACGTGAC
CGCGCACAAATCGCCGTTGCAGAGCCTGAAGTTTGTCGGCCTGCGCGTCAAGGACGGGGTGTGTGACCGCTTCCGCCAGC
GCATGGGCGCGCGCCCCAGCGTCGATACGGTCTCCCCCGACGTGCGCATCTACGCATACCTGACCGAGACCGATTGCACG
ATCTACCTCGACACCACCGGCGAGCCGCTCTTCAAGCGCGGCTGGCGCCAGGAAAAGGGCGAGGCGCCGCTGAAGGAGAA
CCTCGCGGCCGGCATCCTGCGCCTGACGGGCTGGACGGGTGCCCACGGCATGCCGTTCTACGACCCGATGTGCGGCAGCG
GCACGTTCCTCATCGAAGCTGCGCAACGTGCTCTGGGCATTGCACCGGGCGGGCAGCGCGCCTTCGCCTGCGAGTGGTTG
CAGGACCACGACGCCAAATGCTTCAAGCGCCTGCGCGAAGCTGCCGCCGATGCGGCCGCCGCATCCATGCGCCGTGCGCC
GCCGCTGGTGGCCGGCTCCGACATTTCCACCGACATGCTGGCCTATGCCGCCGCCAACTGGCAGCGTGCCGGCCTGCCCG
GCGAGCCGGCGCTCAAGCAAGTCGATGCACGCTTCGGCAAGCCGCCGTTCGAGGCAGCCGGCGTGTTGTTGATGAACCCG
CCCTACGGTGAGCGTATCGCCGTGCGCGGTGCACACGGTTCACGCCGCCGCCGACCGGAAGGCGAGGGCGACGAGGATGG
CACCGTGTTCGAGCGCGCCAGCCGCGCCATGGGTGCCCCGCGCGACGATGGCCGCCGTCCGATGCGCGAGCTGCGGCAAC
CTGAGCCGCCCCCCCCGGTTGACCCCGAGGAAGAGGCCGCCGCCAACGAATTCGCGCAGGCTTTTGCCGGCACGCTCAAG
CGTGAGTTCGCCGGCTGGAAGGCGTTCGTCTTCACCGGCGATCTTTCGATGCCGCGTCGGATGCGGCTGAAGGAATCGCA
GCGCACGCCGCTCTACAACGGCAACATCGAATGCCGCCTGTTCCGCTTCGACATGGTGAAGGGCGGCATGCGTGATCGAC
CCGAACGGCCCGAGCGGCCCGCAAAGGGCGATGCCTCAAACGACAACGCCGACGCATAG

Upstream 100 bases:

>100_bases
CACAAGTTCTACCGCTGGTTCAATGAACTGCCGGTGCTGTTGTTGCTGGCGATTACGATCTTGGTGGTCGTCAAGCCGTT
CTGATTTTTCTGGTTTTCCG

Downstream 100 bases:

>100_bases
GCATCGGCGCTGTGTGACAACTGCGGGCCCGTTTCAGGCGGCCTGCAGATCCTGGAAATGCTCCATCCACGCCGCGAGTT
GATCCGGCGTGAGCTGCGTC

Product: putative RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MATAYFAPCPRGLEQALADELAEIAIRPEVDELAAFEVGRTVPGGVHFFGTQGAAYAVNLHSRIASRVLMRLGSRGYRSE
DDIYALAAAQRWEDYFTPDEMIRVDVTAHKSPLQSLKFVGLRVKDGVCDRFRQRMGARPSVDTVSPDVRIYAYLTETDCT
IYLDTTGEPLFKRGWRQEKGEAPLKENLAAGILRLTGWTGAHGMPFYDPMCGSGTFLIEAAQRALGIAPGGQRAFACEWL
QDHDAKCFKRLREAAADAAAASMRRAPPLVAGSDISTDMLAYAAANWQRAGLPGEPALKQVDARFGKPPFEAAGVLLMNP
PYGERIAVRGAHGSRRRRPEGEGDEDGTVFERASRAMGAPRDDGRRPMRELRQPEPPPPVDPEEEAAANEFAQAFAGTLK
REFAGWKAFVFTGDLSMPRRMRLKESQRTPLYNGNIECRLFRFDMVKGGMRDRPERPERPAKGDASNDNADA

Sequences:

>Translated_472_residues
MATAYFAPCPRGLEQALADELAEIAIRPEVDELAAFEVGRTVPGGVHFFGTQGAAYAVNLHSRIASRVLMRLGSRGYRSE
DDIYALAAAQRWEDYFTPDEMIRVDVTAHKSPLQSLKFVGLRVKDGVCDRFRQRMGARPSVDTVSPDVRIYAYLTETDCT
IYLDTTGEPLFKRGWRQEKGEAPLKENLAAGILRLTGWTGAHGMPFYDPMCGSGTFLIEAAQRALGIAPGGQRAFACEWL
QDHDAKCFKRLREAAADAAAASMRRAPPLVAGSDISTDMLAYAAANWQRAGLPGEPALKQVDARFGKPPFEAAGVLLMNP
PYGERIAVRGAHGSRRRRPEGEGDEDGTVFERASRAMGAPRDDGRRPMRELRQPEPPPPVDPEEEAAANEFAQAFAGTLK
REFAGWKAFVFTGDLSMPRRMRLKESQRTPLYNGNIECRLFRFDMVKGGMRDRPERPERPAKGDASNDNADA
>Mature_471_residues
ATAYFAPCPRGLEQALADELAEIAIRPEVDELAAFEVGRTVPGGVHFFGTQGAAYAVNLHSRIASRVLMRLGSRGYRSED
DIYALAAAQRWEDYFTPDEMIRVDVTAHKSPLQSLKFVGLRVKDGVCDRFRQRMGARPSVDTVSPDVRIYAYLTETDCTI
YLDTTGEPLFKRGWRQEKGEAPLKENLAAGILRLTGWTGAHGMPFYDPMCGSGTFLIEAAQRALGIAPGGQRAFACEWLQ
DHDAKCFKRLREAAADAAAASMRRAPPLVAGSDISTDMLAYAAANWQRAGLPGEPALKQVDARFGKPPFEAAGVLLMNPP
YGERIAVRGAHGSRRRRPEGEGDEDGTVFERASRAMGAPRDDGRRPMRELRQPEPPPPVDPEEEAAANEFAQAFAGTLKR
EFAGWKAFVFTGDLSMPRRMRLKESQRTPLYNGNIECRLFRFDMVKGGMRDRPERPERPAKGDASNDNADA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=293, Percent_Identity=35.4948805460751, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 52084; Mature: 51953

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAYFAPCPRGLEQALADELAEIAIRPEVDELAAFEVGRTVPGGVHFFGTQGAAYAVNL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEEH
HSRIASRVLMRLGSRGYRSEDDIYALAAAQRWEDYFTPDEMIRVDVTAHKSPLQSLKFVG
HHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHC
LRVKDGVCDRFRQRMGARPSVDTVSPDVRIYAYLTETDCTIYLDTTGEPLFKRGWRQEKG
EEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCHHHHHCCCCCCC
EAPLKENLAAGILRLTGWTGAHGMPFYDPMCGSGTFLIEAAQRALGIAPGGQRAFACEWL
CCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHH
QDHDAKCFKRLREAAADAAAASMRRAPPLVAGSDISTDMLAYAAANWQRAGLPGEPALKQ
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHCCCCCCCCCCCHHHHH
VDARFGKPPFEAAGVLLMNPPYGERIAVRGAHGSRRRRPEGEGDEDGTVFERASRAMGAP
HHHHCCCCCHHHCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
RDDGRRPMRELRQPEPPPPVDPEEEAAANEFAQAFAGTLKREFAGWKAFVFTGDLSMPRR
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHH
MRLKESQRTPLYNGNIECRLFRFDMVKGGMRDRPERPERPAKGDASNDNADA
HHHHHHCCCCCCCCCEEEEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATAYFAPCPRGLEQALADELAEIAIRPEVDELAAFEVGRTVPGGVHFFGTQGAAYAVNL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEEH
HSRIASRVLMRLGSRGYRSEDDIYALAAAQRWEDYFTPDEMIRVDVTAHKSPLQSLKFVG
HHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHC
LRVKDGVCDRFRQRMGARPSVDTVSPDVRIYAYLTETDCTIYLDTTGEPLFKRGWRQEKG
EEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCHHHHHCCCCCCC
EAPLKENLAAGILRLTGWTGAHGMPFYDPMCGSGTFLIEAAQRALGIAPGGQRAFACEWL
CCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHH
QDHDAKCFKRLREAAADAAAASMRRAPPLVAGSDISTDMLAYAAANWQRAGLPGEPALKQ
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHCCCCCCCCCCCHHHHH
VDARFGKPPFEAAGVLLMNPPYGERIAVRGAHGSRRRRPEGEGDEDGTVFERASRAMGAP
HHHHCCCCCHHHCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
RDDGRRPMRELRQPEPPPPVDPEEEAAANEFAQAFAGTLKREFAGWKAFVFTGDLSMPRR
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHH
MRLKESQRTPLYNGNIECRLFRFDMVKGGMRDRPERPERPAKGDASNDNADA
HHHHHHCCCCCCCCCEEEEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA