| Definition | Ralstonia pickettii 12J chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010682 |
| Length | 3,942,557 |
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The map label for this gene is clpA [H]
Identifier: 187929803
GI number: 187929803
Start: 2848415
End: 2850703
Strand: Reverse
Name: clpA [H]
Synonym: Rpic_2733
Alternate gene names: 187929803
Gene position: 2850703-2848415 (Counterclockwise)
Preceding gene: 187929804
Following gene: 187929799
Centisome position: 72.31
GC content: 62.95
Gene sequence:
>2289_bases ATGATTGCGCAAGAACTGGAAGTCAGCCTGCACATGGCGTTTGTTGAAGCCCGCCAGGCTCGCCACGAATTCATTACGGT GGAGCATCTGCTGCTTGCGCTGCTGGACAACCCGACGGCCGCCGAGGTGCTTCGCGCCTGCGCTGCCAACATCGAAGACC TCCGCACGCACCTGAAGAACTTCATCGCCGACAACACCCCGGTGGTACCGGGCACGGACGAGGTGGACACGCAGCCGACA CTCGGCTTCCAGCGCGTGATCCAGCGCGCCATCATGCACGTGCAATCCACATCGAACGGGAAGAAGGAAGTGACGGGCGC GAACGTGCTGGTCGCCATCTTCGGCGAGAAGGATTCGCACGCGGTGTACTACCTCCAGCAACAGGGCGTGACCCGGCTGG ACGTCGTCAACTTCATCAGCCACGGCATCCGCAAGGACCAGGCCGAGCCTGCCAAGCAAGGCGAAGGCAACCCCGAGGGC GAAGGCGGCGACGGCAAGGAAAGCCCGCTCGAGCAATTCACGCAAAACCTGAACGCGTTGGCCAAGGCCGGCAAGATCGA TCCCCTGATCGGTCGCGAGCAGGAAGTGGAGCGCGTGGTGCAGGTGCTTTGCCGCCGGCGTAAAAACAACCCGCTGCTGG TGGGCGAGGCCGGTGTCGGCAAGACGGCCATCGCAGAAGGCTTGGCATGGCGCATCACCAAGGGCGAAGTGCCGGACATC CTGGCAAAGTCCGTCGTCTACTCGCTCGACATGGGCGCGCTGCTGGCCGGCACGAAGTACCGCGGCGACTTTGAGCAACG CCTGAAGGGCGTGCTGAAGTCGCTCAAGGACAACCCGAACGCGATTCTGTTCATCGACGAGATCCACACGCTGATTGGCG CAGGCGCCGCTTCGGGCGGCACGCTGGACGCCAGCAACCTGCTCAAGCCCGCGCTGTCGTCGGGCGCGCTCAAGTGCATC GGTGCGACGACGTTCACGGAATACCGCGGCATTTTCGAGAAGGATGCTGCGCTGTCCCGCCGCTTCCAGAAGATCGACGT GGTGGAGCCGACGGTCGACCAGACCGTCCAGATTCTGCGTGGCCTGAAGTCGCGCTTTGAAGAACACCATGGCGTGAAGT ACGCGTCGTCGGCGCTGACCGCCGCGGCTGAATTGTCGGCGCGCTTCATCACCGATCGTCACCTGCCGGACAAGGCGATC GACGTGATCGACGAAGCCGGTGCAGCGCAGCGCATCCTGCCGAAGTCGAAGCAGAAGAAGACCATCGGCAAGGGTGAGAT CGAGGACATCGTCTCGCGTATTGCCCGCGTTCCGCCGCAAAGCGTGTCGCAGGACGACCGCAGCAAGCTGCAGACGCTGG AGCGCGACCTGAAGTCGGTCGTGTTCGGGCAGGACCCGGCCATTGATGCGCTTGCTTCGGCCATCAAGATGTCGCGCGCC GGGCTGGGCAAGACGGACAAGCCGATCGGCTCGTTCCTGTTCAGCGGCCCGACGGGCGTGGGCAAGACGGAAGTCGCCAA GCAGCTCGCGTTCATCCTCGGCATCGAGCTGATCCGTTTCGACATGTCGGAGTACATGGAGCGCCATGCGGTGAGCCGGC TGATCGGCGCGCCTCCGGGCTACGTGGGCTTCGATCAGGGCGGCCTGCTGACGGAAGCCGTGACCAAGAAGCCGCACTGC GTGCTGCTGCTGGACGAGATCGAAAAGGCGCACCCGGACATCTTCAACATCCTGCTGCAGGTGATGGACCACGGCGCGCT GACGGACAACAACGGCCGCAAGGCGGACTTCCGCAACGTGATCATCATCATGACGACCAATGCGGGCGCCGAGACAATGA ACAAGGCAACGATCGGTTTCACCACGGCGCGCGAACAGGGCGACGAGATGGCCGACATCAAGCGCATGTTCACGCCTGAG TTCCGCAACCGTCTGGACGCAACCATCAGCTTCCGCTCGCTGGATGAGGAAATCATCATGCGCGTGGTCGACAAGTTCCT CATGCAGCTGGAAGAGCAACTGCACGAGAAGAAGGTGGATGCCATCTTCACCGAGAAGCTGCGTCGCTTCCTGGCGAAGA AGGGGTTCGACCCGCTGATGGGTGCACGCCCGATGCAGCGCCTGATTCAAGACCTGATCCGCAAGGCTCTGGCCGACGAA CTGCTGTTCGGCAAGCTGGTCAACGGCGGCAAGGTGATCGTGGATCTGGACGATGCAGACGCGGTGAAGCTGGAGTTCTC CGAGAACGAAGCGCCGCCGGCACCTGCCCAGGAAGAAGCGGAAGCGTGA
Upstream 100 bases:
>100_bases GCGGTCTCTATACCCGGGATATCGCCGCGACGAAAGTGGAATTGGTGTCAACCCATGCACGGCAAGCGGGGCATCCGCTG CAGTGTGTGATGGAGGAAGC
Downstream 100 bases:
>100_bases CGAGTTTGTCGCACCAAACAAAAGGCCCGGAAGTTCCGGGCCTTTTTTATATGTGGGCGATATTGTCAGTGACGCCCGGT GCTGCCGAAACCACCGGCAC
Product: ATP-dependent Clp protease, ATP-binding subunit clpA
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 762; Mature: 762
Protein sequence:
>762_residues MIAQELEVSLHMAFVEARQARHEFITVEHLLLALLDNPTAAEVLRACAANIEDLRTHLKNFIADNTPVVPGTDEVDTQPT LGFQRVIQRAIMHVQSTSNGKKEVTGANVLVAIFGEKDSHAVYYLQQQGVTRLDVVNFISHGIRKDQAEPAKQGEGNPEG EGGDGKESPLEQFTQNLNALAKAGKIDPLIGREQEVERVVQVLCRRRKNNPLLVGEAGVGKTAIAEGLAWRITKGEVPDI LAKSVVYSLDMGALLAGTKYRGDFEQRLKGVLKSLKDNPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGALKCI GATTFTEYRGIFEKDAALSRRFQKIDVVEPTVDQTVQILRGLKSRFEEHHGVKYASSALTAAAELSARFITDRHLPDKAI DVIDEAGAAQRILPKSKQKKTIGKGEIEDIVSRIARVPPQSVSQDDRSKLQTLERDLKSVVFGQDPAIDALASAIKMSRA GLGKTDKPIGSFLFSGPTGVGKTEVAKQLAFILGIELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAVTKKPHC VLLLDEIEKAHPDIFNILLQVMDHGALTDNNGRKADFRNVIIIMTTNAGAETMNKATIGFTTAREQGDEMADIKRMFTPE FRNRLDATISFRSLDEEIIMRVVDKFLMQLEEQLHEKKVDAIFTEKLRRFLAKKGFDPLMGARPMQRLIQDLIRKALADE LLFGKLVNGGKVIVDLDDADAVKLEFSENEAPPAPAQEEAEA
Sequences:
>Translated_762_residues MIAQELEVSLHMAFVEARQARHEFITVEHLLLALLDNPTAAEVLRACAANIEDLRTHLKNFIADNTPVVPGTDEVDTQPT LGFQRVIQRAIMHVQSTSNGKKEVTGANVLVAIFGEKDSHAVYYLQQQGVTRLDVVNFISHGIRKDQAEPAKQGEGNPEG EGGDGKESPLEQFTQNLNALAKAGKIDPLIGREQEVERVVQVLCRRRKNNPLLVGEAGVGKTAIAEGLAWRITKGEVPDI LAKSVVYSLDMGALLAGTKYRGDFEQRLKGVLKSLKDNPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGALKCI GATTFTEYRGIFEKDAALSRRFQKIDVVEPTVDQTVQILRGLKSRFEEHHGVKYASSALTAAAELSARFITDRHLPDKAI DVIDEAGAAQRILPKSKQKKTIGKGEIEDIVSRIARVPPQSVSQDDRSKLQTLERDLKSVVFGQDPAIDALASAIKMSRA GLGKTDKPIGSFLFSGPTGVGKTEVAKQLAFILGIELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAVTKKPHC VLLLDEIEKAHPDIFNILLQVMDHGALTDNNGRKADFRNVIIIMTTNAGAETMNKATIGFTTAREQGDEMADIKRMFTPE FRNRLDATISFRSLDEEIIMRVVDKFLMQLEEQLHEKKVDAIFTEKLRRFLAKKGFDPLMGARPMQRLIQDLIRKALADE LLFGKLVNGGKVIVDLDDADAVKLEFSENEAPPAPAQEEAEA >Mature_762_residues MIAQELEVSLHMAFVEARQARHEFITVEHLLLALLDNPTAAEVLRACAANIEDLRTHLKNFIADNTPVVPGTDEVDTQPT LGFQRVIQRAIMHVQSTSNGKKEVTGANVLVAIFGEKDSHAVYYLQQQGVTRLDVVNFISHGIRKDQAEPAKQGEGNPEG EGGDGKESPLEQFTQNLNALAKAGKIDPLIGREQEVERVVQVLCRRRKNNPLLVGEAGVGKTAIAEGLAWRITKGEVPDI LAKSVVYSLDMGALLAGTKYRGDFEQRLKGVLKSLKDNPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGALKCI GATTFTEYRGIFEKDAALSRRFQKIDVVEPTVDQTVQILRGLKSRFEEHHGVKYASSALTAAAELSARFITDRHLPDKAI DVIDEAGAAQRILPKSKQKKTIGKGEIEDIVSRIARVPPQSVSQDDRSKLQTLERDLKSVVFGQDPAIDALASAIKMSRA GLGKTDKPIGSFLFSGPTGVGKTEVAKQLAFILGIELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAVTKKPHC VLLLDEIEKAHPDIFNILLQVMDHGALTDNNGRKADFRNVIIIMTTNAGAETMNKATIGFTTAREQGDEMADIKRMFTPE FRNRLDATISFRSLDEEIIMRVVDKFLMQLEEQLHEKKVDAIFTEKLRRFLAKKGFDPLMGARPMQRLIQDLIRKALADE LLFGKLVNGGKVIVDLDDADAVKLEFSENEAPPAPAQEEAEA
Specific function: ATP-dependent specificity component of the ClpP protease. It directs the protease to specific substrates. The primary function of the ClpA-ClpP complex appears to be the degradation of unfolded or abnormal proteins [H]
COG id: COG0542
COG function: function code O; ATPases with chaperone activity, ATP-binding subunit
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the clpA/clpB family [H]
Homologues:
Organism=Homo sapiens, GI13540606, Length=337, Percent_Identity=29.9703264094956, Blast_Score=150, Evalue=3e-36, Organism=Escherichia coli, GI1787109, Length=738, Percent_Identity=63.4146341463415, Blast_Score=976, Evalue=0.0, Organism=Escherichia coli, GI1788943, Length=399, Percent_Identity=45.6140350877193, Blast_Score=308, Evalue=9e-85, Organism=Saccharomyces cerevisiae, GI6320464, Length=253, Percent_Identity=57.3122529644269, Blast_Score=283, Evalue=8e-77, Organism=Saccharomyces cerevisiae, GI6323002, Length=269, Percent_Identity=50.5576208178439, Blast_Score=261, Evalue=4e-70,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003593 - InterPro: IPR013093 - InterPro: IPR003959 - InterPro: IPR018368 - InterPro: IPR001270 - InterPro: IPR019489 - InterPro: IPR004176 - InterPro: IPR013461 - InterPro: IPR023150 [H]
Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]
EC number: NA
Molecular weight: Translated: 83523; Mature: 83523
Theoretical pI: Translated: 6.23; Mature: 6.23
Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIAQELEVSLHMAFVEARQARHEFITVEHLLLALLDNPTAAEVLRACAANIEDLRTHLKN CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH FIADNTPVVPGTDEVDTQPTLGFQRVIQRAIMHVQSTSNGKKEVTGANVLVAIFGEKDSH HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCC AVYYLQQQGVTRLDVVNFISHGIRKDQAEPAKQGEGNPEGEGGDGKESPLEQFTQNLNAL EEEEEHHCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH AKAGKIDPLIGREQEVERVVQVLCRRRKNNPLLVGEAGVGKTAIAEGLAWRITKGEVPDI HHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEECCCCCHHH LAKSVVYSLDMGALLAGTKYRGDFEQRLKGVLKSLKDNPNAILFIDEIHTLIGAGAASGG HHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCC TLDASNLLKPALSSGALKCIGATTFTEYRGIFEKDAALSRRFQKIDVVEPTVDQTVQILR CCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH GLKSRFEEHHGVKYASSALTAAAELSARFITDRHLPDKAIDVIDEAGAAQRILPKSKQKK HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCHHHH TIGKGEIEDIVSRIARVPPQSVSQDDRSKLQTLERDLKSVVFGQDPAIDALASAIKMSRA CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC GLGKTDKPIGSFLFSGPTGVGKTEVAKQLAFILGIELIRFDMSEYMERHAVSRLIGAPPG CCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC YVGFDQGGLLTEAVTKKPHCVLLLDEIEKAHPDIFNILLQVMDHGALTDNNGRKADFRNV CCCCCCCCCHHHHHCCCCCEEEEHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCE IIIMTTNAGAETMNKATIGFTTAREQGDEMADIKRMFTPEFRNRLDATISFRSLDEEIIM EEEEECCCCHHHHCCCCEEEEHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHH RVVDKFLMQLEEQLHEKKVDAIFTEKLRRFLAKKGFDPLMGARPMQRLIQDLIRKALADE HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH LLFGKLVNGGKVIVDLDDADAVKLEFSENEAPPAPAQEEAEA HHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCC >Mature Secondary Structure MIAQELEVSLHMAFVEARQARHEFITVEHLLLALLDNPTAAEVLRACAANIEDLRTHLKN CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH FIADNTPVVPGTDEVDTQPTLGFQRVIQRAIMHVQSTSNGKKEVTGANVLVAIFGEKDSH HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCC AVYYLQQQGVTRLDVVNFISHGIRKDQAEPAKQGEGNPEGEGGDGKESPLEQFTQNLNAL EEEEEHHCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH AKAGKIDPLIGREQEVERVVQVLCRRRKNNPLLVGEAGVGKTAIAEGLAWRITKGEVPDI HHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEECCCCCHHH LAKSVVYSLDMGALLAGTKYRGDFEQRLKGVLKSLKDNPNAILFIDEIHTLIGAGAASGG HHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCC TLDASNLLKPALSSGALKCIGATTFTEYRGIFEKDAALSRRFQKIDVVEPTVDQTVQILR CCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH GLKSRFEEHHGVKYASSALTAAAELSARFITDRHLPDKAIDVIDEAGAAQRILPKSKQKK HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCHHHH TIGKGEIEDIVSRIARVPPQSVSQDDRSKLQTLERDLKSVVFGQDPAIDALASAIKMSRA CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC GLGKTDKPIGSFLFSGPTGVGKTEVAKQLAFILGIELIRFDMSEYMERHAVSRLIGAPPG CCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC YVGFDQGGLLTEAVTKKPHCVLLLDEIEKAHPDIFNILLQVMDHGALTDNNGRKADFRNV CCCCCCCCCHHHHHCCCCCEEEEHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCE IIIMTTNAGAETMNKATIGFTTAREQGDEMADIKRMFTPEFRNRLDATISFRSLDEEIIM EEEEECCCCHHHHCCCCEEEEHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHH RVVDKFLMQLEEQLHEKKVDAIFTEKLRRFLAKKGFDPLMGARPMQRLIQDLIRKALADE HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH LLFGKLVNGGKVIVDLDDADAVKLEFSENEAPPAPAQEEAEA HHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]