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The map label for this gene is cobS

Identifier: 187929733

GI number: 187929733

Start: 2775712

End: 2776482

Strand: Reverse

Name: cobS

Synonym: Rpic_2662

Alternate gene names: 187929733

Gene position: 2776482-2775712 (Counterclockwise)

Preceding gene: 187929734

Following gene: 187929732

Centisome position: 70.42

GC content: 65.24

Gene sequence:

>771_bases
ATGAACGCGTTGCGCGAACATTGGCAGGCGCTGTGGACGGCCGTCGGGTATTTCACCCGCATCCCCGTGCCGGCGTCGGT
TGGGTTCTCGCAAGCGGGCTTGAATCGCGCGGCGCGCTACTTTCCGCTGATCGGCTGGTTGGTGGGCGCCGTGGCAGCGG
CGGTGTATTGGCTTGTGTTGCGCACGGTGCCCGCGCAAGGTGTGGCGGTGGCCGTGTCGATGGGGGCTACGCTATTGCTG
ACCGGCGCGTTTCACGAGGACGGGCTGGCCGATTGCGCCGACGGCTTTGGCGGCGGCTACACGCCTGAAGACCGGCTGCG
CATCATGCACGATTCGCGCATCGGCGCGTTCGGGGCGATTGCCGTGTGCATGGCGTTGCTGCTGAAGTGGCAATTGCTGA
GCGCGATGGCGCCGCACAGCATAGGCATCCTCGTTGCAATGGTGGCTGCGCATGCTGCCAGCCGGGCCGCAGCTGTCAGC
CACCTGCTCACGCATGACTACGTGCGGGCGGAAGGCAAGGCCAAGCCAGTTGCGCAACGCATGCGATGGAGTGACGCGTT
GTGGGCTGGCGTATTCGGTGTCGTGCCGTTGCTGTGGTTCGGGCCGATGTGCGCGGCGCTCATCGTTGTCGGCCTGATCC
TTGCGCGCTGGCTGCTGGGTCGCTATTTCGTGCGCCGCATCGGCGGCATCACAGGCGATTGCCTGGGCATGGCACAGCAG
ATTTTCGAGCTGCTGATCCTGTGGGGGTTGCTGGCATGGATGTCATCCTGA

Upstream 100 bases:

>100_bases
GCGGCGCGGTGTTGGCGTATCCGGTGGTCGTGTCGGCGGTGGCGTTCCTGCGCGAAATGGCGACCTTCGCCAGCGCAGGC
GTGAGCGAGCGCGCATCGCC

Downstream 100 bases:

>100_bases
TCCGCCACGCGCGGCCCGCCATGACGGCGGGCCTCTGCTATGGCCGCACCGATCTGGAGTTGGTCTCGCCCATGGACCCG
GAGCCGTCGGCCATCGTCGA

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MNALREHWQALWTAVGYFTRIPVPASVGFSQAGLNRAARYFPLIGWLVGAVAAAVYWLVLRTVPAQGVAVAVSMGATLLL
TGAFHEDGLADCADGFGGGYTPEDRLRIMHDSRIGAFGAIAVCMALLLKWQLLSAMAPHSIGILVAMVAAHAASRAAAVS
HLLTHDYVRAEGKAKPVAQRMRWSDALWAGVFGVVPLLWFGPMCAALIVVGLILARWLLGRYFVRRIGGITGDCLGMAQQ
IFELLILWGLLAWMSS

Sequences:

>Translated_256_residues
MNALREHWQALWTAVGYFTRIPVPASVGFSQAGLNRAARYFPLIGWLVGAVAAAVYWLVLRTVPAQGVAVAVSMGATLLL
TGAFHEDGLADCADGFGGGYTPEDRLRIMHDSRIGAFGAIAVCMALLLKWQLLSAMAPHSIGILVAMVAAHAASRAAAVS
HLLTHDYVRAEGKAKPVAQRMRWSDALWAGVFGVVPLLWFGPMCAALIVVGLILARWLLGRYFVRRIGGITGDCLGMAQQ
IFELLILWGLLAWMSS
>Mature_256_residues
MNALREHWQALWTAVGYFTRIPVPASVGFSQAGLNRAARYFPLIGWLVGAVAAAVYWLVLRTVPAQGVAVAVSMGATLLL
TGAFHEDGLADCADGFGGGYTPEDRLRIMHDSRIGAFGAIAVCMALLLKWQLLSAMAPHSIGILVAMVAAHAASRAAAVS
HLLTHDYVRAEGKAKPVAQRMRWSDALWAGVFGVVPLLWFGPMCAALIVVGLILARWLLGRYFVRRIGGITGDCLGMAQQ
IFELLILWGLLAWMSS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=249, Percent_Identity=34.9397590361446, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_RALPJ (B2UAJ1)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001900220.1
- GeneID:   6288336
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_2662
- HOGENOM:   HBG729392
- OMA:   HDSRIGA
- ProtClustDB:   PRK00235
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27542; Mature: 27542

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x407fc184)-; HASH(0x17e24088)-; HASH(0x17ec7d50)-; HASH(0x177a0d20)-; HASH(0x17e2f4fc)-; HASH(0x17bf0748)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALREHWQALWTAVGYFTRIPVPASVGFSQAGLNRAARYFPLIGWLVGAVAAAVYWLVL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTVPAQGVAVAVSMGATLLLTGAFHEDGLADCADGFGGGYTPEDRLRIMHDSRIGAFGAI
HHCCCCCHHHHHHHCHHHHEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHH
AVCMALLLKWQLLSAMAPHSIGILVAMVAAHAASRAAAVSHLLTHDYVRAEGKAKPVAQR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
MRWSDALWAGVFGVVPLLWFGPMCAALIVVGLILARWLLGRYFVRRIGGITGDCLGMAQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IFELLILWGLLAWMSS
HHHHHHHHHHHHHHCH
>Mature Secondary Structure
MNALREHWQALWTAVGYFTRIPVPASVGFSQAGLNRAARYFPLIGWLVGAVAAAVYWLVL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTVPAQGVAVAVSMGATLLLTGAFHEDGLADCADGFGGGYTPEDRLRIMHDSRIGAFGAI
HHCCCCCHHHHHHHCHHHHEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHH
AVCMALLLKWQLLSAMAPHSIGILVAMVAAHAASRAAAVSHLLTHDYVRAEGKAKPVAQR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
MRWSDALWAGVFGVVPLLWFGPMCAALIVVGLILARWLLGRYFVRRIGGITGDCLGMAQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IFELLILWGLLAWMSS
HHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA