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The map label for this gene is gloB [H]

Identifier: 187929604

GI number: 187929604

Start: 2643760

End: 2644533

Strand: Reverse

Name: gloB [H]

Synonym: Rpic_2530

Alternate gene names: 187929604

Gene position: 2644533-2643760 (Counterclockwise)

Preceding gene: 187929605

Following gene: 187929598

Centisome position: 67.08

GC content: 61.5

Gene sequence:

>774_bases
ATGTTGACACTTCGACCGTTGCCGGCGTTCGCTGACAACTACATTTGGGTATTGCATGATGAGCGACACGCTTGGGTCGT
GGATCCTGGGGAGTCTGCACAAGTGCTCGACTATCTGCAGAAGCACGGTCTGGTTCTGCAGGGGATACTTCTGACGCACC
ATCACTCCGACCATGTCGGCGGAGTGCAGGCGCTGCGCCAGCAAACCGGCGCTGCTGTCTACGGTCCGGCGGGCGAGCCG
ATTCCCGAGCCTGCGCGCCGGCTGAGGCAGGGCGATCGGGTGGATGTCTTGGAGCAGCAGTTTGAGGTGATCGATGTGCC
GGGACATACCGCCGGCCACATCGCTTATTTCAGTGCTGCCTGCCCCGATGGACCGCTCTTGTTTTGCGGGGACACGCTGT
TTTCGGGAGGCTGCGGAAGGTTGTTCGAGGGCACACCGGAGCAGATGCTGGCATCTCTCGATACGCTCGCGGCCTTGCCC
GCGCAGACGCAAGTGTGTCCCGCGCATGAATACACGCTAGCCAACCTGCGGTTCGCGCAGACGGTCGAACCATCTAACGA
AGCTCTGGCCCGCCACGTGCCCTATTGCGAGATGTTGCGCGCGCAAGGATTGCCGACGCTACCGAGCCGCCTTGGGCTCG
AGCGTCAGATCAACCCGTTCCTTCGCTCGCGCGAACCCAGCGTGCGGCAGTCGCTCAGTCGGTTGCCGTCAGCCAGTCTT
GGCGATGTGCACGTCTTTGCCGCGCTTCGCGAATGGAAAAACAACTTTCAATGA

Upstream 100 bases:

>100_bases
GTCGTCGGTCAACTGGCCGCGCTGGCGGCGAGCGATGAACTCAACGCCGCGCAAGCCGAGGCGCCGTCGGCGCTGGAATT
TGTCTGAAGGCTGGCTCCCC

Downstream 100 bases:

>100_bases
TCGCGCGCTGAGCGTGGCGCATCGGGTCGTTACGTGTCACAGGGTTCTGCTCGCCCCCTGCGCAGGCGGTTCTGTGGCGA
TATGACGGCGGCGAGGAACG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLTLRPLPAFADNYIWVLHDERHAWVVDPGESAQVLDYLQKHGLVLQGILLTHHHSDHVGGVQALRQQTGAAVYGPAGEP
IPEPARRLRQGDRVDVLEQQFEVIDVPGHTAGHIAYFSAACPDGPLLFCGDTLFSGGCGRLFEGTPEQMLASLDTLAALP
AQTQVCPAHEYTLANLRFAQTVEPSNEALARHVPYCEMLRAQGLPTLPSRLGLERQINPFLRSREPSVRQSLSRLPSASL
GDVHVFAALREWKNNFQ

Sequences:

>Translated_257_residues
MLTLRPLPAFADNYIWVLHDERHAWVVDPGESAQVLDYLQKHGLVLQGILLTHHHSDHVGGVQALRQQTGAAVYGPAGEP
IPEPARRLRQGDRVDVLEQQFEVIDVPGHTAGHIAYFSAACPDGPLLFCGDTLFSGGCGRLFEGTPEQMLASLDTLAALP
AQTQVCPAHEYTLANLRFAQTVEPSNEALARHVPYCEMLRAQGLPTLPSRLGLERQINPFLRSREPSVRQSLSRLPSASL
GDVHVFAALREWKNNFQ
>Mature_257_residues
MLTLRPLPAFADNYIWVLHDERHAWVVDPGESAQVLDYLQKHGLVLQGILLTHHHSDHVGGVQALRQQTGAAVYGPAGEP
IPEPARRLRQGDRVDVLEQQFEVIDVPGHTAGHIAYFSAACPDGPLLFCGDTLFSGGCGRLFEGTPEQMLASLDTLAALP
AQTQVCPAHEYTLANLRFAQTVEPSNEALARHVPYCEMLRAQGLPTLPSRLGLERQINPFLRSREPSVRQSLSRLPSASL
GDVHVFAALREWKNNFQ

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=36.8821292775665, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=36.8821292775665, Blast_Score=165, Evalue=3e-41,
Organism=Homo sapiens, GI14150041, Length=234, Percent_Identity=40.1709401709402, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI21703352, Length=251, Percent_Identity=39.8406374501992, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI116642887, Length=251, Percent_Identity=39.8406374501992, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI46361987, Length=191, Percent_Identity=40.8376963350785, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1786406, Length=262, Percent_Identity=44.2748091603053, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1787158, Length=190, Percent_Identity=33.6842105263158, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17536925, Length=264, Percent_Identity=36.7424242424242, Blast_Score=161, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6320478, Length=268, Percent_Identity=30.5970149253731, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324614, Length=269, Percent_Identity=28.6245353159851, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356335, Length=263, Percent_Identity=38.4030418250951, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24667711, Length=263, Percent_Identity=38.4030418250951, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24667703, Length=263, Percent_Identity=38.4030418250951, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI221330176, Length=173, Percent_Identity=30.0578034682081, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28317; Mature: 28317

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00743 BETA_LACTAMASE_B_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLRPLPAFADNYIWVLHDERHAWVVDPGESAQVLDYLQKHGLVLQGILLTHHHSDHVG
CCCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHCCCEEEEHHHHCCCCCCHH
GVQALRQQTGAAVYGPAGEPIPEPARRLRQGDRVDVLEQQFEVIDVPGHTAGHIAYFSAA
HHHHHHHHCCCEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCEEEEECC
CPDGPLLFCGDTLFSGGCGRLFEGTPEQMLASLDTLAALPAQTQVCPAHEYTLANLRFAQ
CCCCCEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEC
TVEPSNEALARHVPYCEMLRAQGLPTLPSRLGLERQINPFLRSREPSVRQSLSRLPSASL
CCCCCHHHHHHHCCHHHHHHHCCCCCCHHHHCCHHHCCHHHHCCCHHHHHHHHHCCCCCC
GDVHVFAALREWKNNFQ
CHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTLRPLPAFADNYIWVLHDERHAWVVDPGESAQVLDYLQKHGLVLQGILLTHHHSDHVG
CCCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHCCCEEEEHHHHCCCCCCHH
GVQALRQQTGAAVYGPAGEPIPEPARRLRQGDRVDVLEQQFEVIDVPGHTAGHIAYFSAA
HHHHHHHHCCCEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCEEEEECC
CPDGPLLFCGDTLFSGGCGRLFEGTPEQMLASLDTLAALPAQTQVCPAHEYTLANLRFAQ
CCCCCEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEC
TVEPSNEALARHVPYCEMLRAQGLPTLPSRLGLERQINPFLRSREPSVRQSLSRLPSASL
CCCCCHHHHHHHCCHHHHHHHCCCCCCHHHHCCHHHCCHHHHCCCHHHHHHHHHCCCCCC
GDVHVFAALREWKNNFQ
CHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA