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The map label for this gene is hipO [H]

Identifier: 187929598

GI number: 187929598

Start: 2638069

End: 2639334

Strand: Reverse

Name: hipO [H]

Synonym: Rpic_2524

Alternate gene names: 187929598

Gene position: 2639334-2638069 (Counterclockwise)

Preceding gene: 187929604

Following gene: 187929597

Centisome position: 66.94

GC content: 61.06

Gene sequence:

>1266_bases
ATGAACGCACTCGTCAACGCGCTGCACGGCCGCAGCGACACGCTGCTGGCCGAGCTCGAAGCCATTTACAAAGACCTCCA
TCAGCACCCCGAGCTTTCCATGCAGGAAGTCCGGACCGCCAAGATCGCCGCCGACCATATCGAGGCGCTGGGCTATGAAG
TCACACGCGATGTCGGTATCACGGGCGTAGTCGGCGTGCTGCGCAATGGAGATGGCCCTACGGTGATGCTACGCGCGGAC
ATGGACGCGCTGCCCATGGCGGAAAATACCGGCTTGCCTTACGCGAGCACCGTCAAGGCCAAGGATGAGGAGGGCGTTGA
AGTCGATGTTGCCCATTCATGCGGACATGACATGCATGTGACCTGGCTGATCGGTGCTGCGCGCGTTCTCGCAGAGCACC
GCGACGCCTGGCGGGGTACGGTCATGGTGGTGTTCCAACCGGGCGAAGAGGTAGGGCGCGGCGCCGCCAGCATGGTGGAC
GACTGGGGCACCGAACGCTTTCCCAAGCCGGACATCATCCTGGGTCAGCATGTCATGGTCGGGGCCAGCGGCACGGTCAG
TTACCGGCCCGGCGTCATTCTGTCAGCGGGCGATAGCCTGAAAATCCGGCTGTTCGGTCGCGGCTCGCACGGCTCTCAGC
CGCAGACGTCGATTGATCCGGTCATCATGGCCGCCTCCACCACGTTGCGTTTGCAGACCATTGTCTCTCGTGAAATTTCG
CCGATGGACAATGCCGTGCTGACCATTGGAGCGCTGCAGGCCGGCACCAAGGAAAACATCATCCCGGACGAAGCCACAAT
CAAGCTCAACATGCGCACCTTCGACGAAGATGTGCGCGAATACATGCTGGCTTCTATCAAACGGATTTGCTGTGCGGAGT
GCGATGCATCGAACGCCGAGCGGCCGCCGGAGTTCACGACCCTCAGCGCGTATCCACTCACCGAAAACGACGCGGCCGCC
ACGCAGCGGCTGGCAGAGGCTTTCAAGGCCCAGTTCGGCGACAAGGCTTACGAGACGAAGCCTGCGTCGGCCAGTGAAGA
CTTCAGCATCTTTGGGCGTGCCTGGAATGTGCCTTACGTCTTCTGGTTCATCGGCGGAACCGACCCTCAAATCTATGCAC
AAGCCGAAGCGGCCAGGCAGGTCAACAAAATACCGAGTAACCATTCACCCAAGTTCGCACCGGTCATTCATCCGACGCTG
GAAACCGGCCTGCACGCGATGCTCACGGCGGCCTCGGCATGGCTTTGCACTTCTCCGGCAACATGA

Upstream 100 bases:

>100_bases
ATAGCCCTTGTGGGGTCCGCGCTGAAGAGGGCGTCCCCATGGAGTCTTCAGGGCGGCAACTGAATGTGTATGCGAAGCAC
AAGGCATCCCGGGAGTTTTT

Downstream 100 bases:

>100_bases
TGGAGCACAACCTCTTTGTCCTGGTCGACTTGCTGGGCACCCTCGCCTTCGCGGTCAGTGGTGCCCTCGCAGCCGAACAG
AAGCGGCTCGACCTGTTTGG

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNALVNALHGRSDTLLAELEAIYKDLHQHPELSMQEVRTAKIAADHIEALGYEVTRDVGITGVVGVLRNGDGPTVMLRAD
MDALPMAENTGLPYASTVKAKDEEGVEVDVAHSCGHDMHVTWLIGAARVLAEHRDAWRGTVMVVFQPGEEVGRGAASMVD
DWGTERFPKPDIILGQHVMVGASGTVSYRPGVILSAGDSLKIRLFGRGSHGSQPQTSIDPVIMAASTTLRLQTIVSREIS
PMDNAVLTIGALQAGTKENIIPDEATIKLNMRTFDEDVREYMLASIKRICCAECDASNAERPPEFTTLSAYPLTENDAAA
TQRLAEAFKAQFGDKAYETKPASASEDFSIFGRAWNVPYVFWFIGGTDPQIYAQAEAARQVNKIPSNHSPKFAPVIHPTL
ETGLHAMLTAASAWLCTSPAT

Sequences:

>Translated_421_residues
MNALVNALHGRSDTLLAELEAIYKDLHQHPELSMQEVRTAKIAADHIEALGYEVTRDVGITGVVGVLRNGDGPTVMLRAD
MDALPMAENTGLPYASTVKAKDEEGVEVDVAHSCGHDMHVTWLIGAARVLAEHRDAWRGTVMVVFQPGEEVGRGAASMVD
DWGTERFPKPDIILGQHVMVGASGTVSYRPGVILSAGDSLKIRLFGRGSHGSQPQTSIDPVIMAASTTLRLQTIVSREIS
PMDNAVLTIGALQAGTKENIIPDEATIKLNMRTFDEDVREYMLASIKRICCAECDASNAERPPEFTTLSAYPLTENDAAA
TQRLAEAFKAQFGDKAYETKPASASEDFSIFGRAWNVPYVFWFIGGTDPQIYAQAEAARQVNKIPSNHSPKFAPVIHPTL
ETGLHAMLTAASAWLCTSPAT
>Mature_421_residues
MNALVNALHGRSDTLLAELEAIYKDLHQHPELSMQEVRTAKIAADHIEALGYEVTRDVGITGVVGVLRNGDGPTVMLRAD
MDALPMAENTGLPYASTVKAKDEEGVEVDVAHSCGHDMHVTWLIGAARVLAEHRDAWRGTVMVVFQPGEEVGRGAASMVD
DWGTERFPKPDIILGQHVMVGASGTVSYRPGVILSAGDSLKIRLFGRGSHGSQPQTSIDPVIMAASTTLRLQTIVSREIS
PMDNAVLTIGALQAGTKENIIPDEATIKLNMRTFDEDVREYMLASIKRICCAECDASNAERPPEFTTLSAYPLTENDAAA
TQRLAEAFKAQFGDKAYETKPASASEDFSIFGRAWNVPYVFWFIGGTDPQIYAQAEAARQVNKIPSNHSPKFAPVIHPTL
ETGLHAMLTAASAWLCTSPAT

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=316, Percent_Identity=25.3164556962025, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 45540; Mature: 45540

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALVNALHGRSDTLLAELEAIYKDLHQHPELSMQEVRTAKIAADHIEALGYEVTRDVGI
CCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCC
TGVVGVLRNGDGPTVMLRADMDALPMAENTGLPYASTVKAKDEEGVEVDVAHSCGHDMHV
CEEEHEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEEEEHHCCCCCCEE
TWLIGAARVLAEHRDAWRGTVMVVFQPGEEVGRGAASMVDDWGTERFPKPDIILGQHVMV
EHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHHHCCCCCCCCCCEEECCEEEE
GASGTVSYRPGVILSAGDSLKIRLFGRGSHGSQPQTSIDPVIMAASTTLRLQTIVSREIS
ECCCCEEECCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCC
PMDNAVLTIGALQAGTKENIIPDEATIKLNMRTFDEDVREYMLASIKRICCAECDASNAE
CCCCCEEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RPPEFTTLSAYPLTENDAAATQRLAEAFKAQFGDKAYETKPASASEDFSIFGRAWNVPYV
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCEEEEEEECCCEE
FWFIGGTDPQIYAQAEAARQVNKIPSNHSPKFAPVIHPTLETGLHAMLTAASAWLCTSPA
EEEECCCCCCEEEHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHEEECCCC
T
C
>Mature Secondary Structure
MNALVNALHGRSDTLLAELEAIYKDLHQHPELSMQEVRTAKIAADHIEALGYEVTRDVGI
CCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCC
TGVVGVLRNGDGPTVMLRADMDALPMAENTGLPYASTVKAKDEEGVEVDVAHSCGHDMHV
CEEEHEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEEEEHHCCCCCCEE
TWLIGAARVLAEHRDAWRGTVMVVFQPGEEVGRGAASMVDDWGTERFPKPDIILGQHVMV
EHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHHHCCCCCCCCCCEEECCEEEE
GASGTVSYRPGVILSAGDSLKIRLFGRGSHGSQPQTSIDPVIMAASTTLRLQTIVSREIS
ECCCCEEECCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCC
PMDNAVLTIGALQAGTKENIIPDEATIKLNMRTFDEDVREYMLASIKRICCAECDASNAE
CCCCCEEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RPPEFTTLSAYPLTENDAAATQRLAEAFKAQFGDKAYETKPASASEDFSIFGRAWNVPYV
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCEEEEEEECCCEE
FWFIGGTDPQIYAQAEAARQVNKIPSNHSPKFAPVIHPTLETGLHAMLTAASAWLCTSPA
EEEECCCCCCEEEHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHEEECCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]