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The map label for this gene is fadE25 [H]

Identifier: 187929275

GI number: 187929275

Start: 2305790

End: 2306920

Strand: Reverse

Name: fadE25 [H]

Synonym: Rpic_2196

Alternate gene names: 187929275

Gene position: 2306920-2305790 (Counterclockwise)

Preceding gene: 187929276

Following gene: 187929274

Centisome position: 58.51

GC content: 64.19

Gene sequence:

>1131_bases
ATGGATTTCTCGTTCACCGACGAACAGAAGCAACTTGCCGATGCGGTGCGCCGCTTCATTGAGAAGGATTACGGCTTTGA
AGCGCGCAACAAGGTGGTGTATTCGCCGGAAGGTGTGTCGCAGAGCCATTGGGATGCACTGGCAGAACTGGGCCTGACCG
CGCTGCCAGTGCCCGAAGCACAAGGCGGTTTTGACGGCCGTGCGATGGACTTGCTGGTGGTGATGCAGGAACTGGGCCGC
GGCCTGGTCGTGGAGCCGTATCAGGCCACCGTGCTGGGTGCCCAGGCGCTGAAGCTCGCGGGCGGCCAGGAAGGGTTGCT
GGAAGAAGTCGCTGGCGGCGCCGTGAAGCTGGCCATTGCCTTTGGCGAGCGCCAATCGCGTTACGAGCTTTTCAATGTCA
CCACGCGTGCTGCGCAGCAGGGCAGCGGCTGGACGCTCACCGGCGAGAAGGCGGTTGTGGTGCATGGCGCGCAGGCAGAC
AAGCTGATCGTCTCGGCGCGCACGGGCGGCGAAGCGCGTGATACGTCGGGCCTGTCGCTGTTCGTGGTCGACCGTGATGC
CGCCGGCGTGACGGTCAAGGATTACCGCACGATCGACAACCTGCACGCTGCGGATATCCGCTTCGACAACGCGCCGGCAA
CGCTGCTGGGCAAGGCCGGCGAAGCGTGGGAGACCATCGACGCCGTAGCCGATCTTGGCTGCGTGCTGTTGTGCGCAGAA
GCGATCGGCCTGATCGATGCGCTGAACGCCGCAACGCTGGAGTACACCAAGACCCGCCAGCAGTTTGGCGTGCCCATCGC
GCGCTTCCAGGCGCTGCAGCATCGCATGGTCGACATGTTCATCCATGGCGAGCAGGCGCGTTCGATCACGTATCTTGCCG
CTGCGCATTTTGAAGATGGCGATGTCGAGGCGCGCCGCCGCTATGTGTCTGCTGCCAAGGCACGTGTCGGCCAGGCGGCG
CGTGACGTTGGCCAGGAAGCGGTGCAACTGCACGGCGGCATGGGCGTGACGAACGAACTGCCCGCCGCGCACATGTTCAA
GCGGCTGACGATGATCAACACGACGCTGGGCGACGTGGATCATCACCTCGCACGCTTCACATCGCTGCCCGGCTTCCAGC
AGGCGGCGTAA

Upstream 100 bases:

>100_bases
CGATCTACGGCGGGTCGAACGAGATCCAGCGCAACATCATCTCGCAGATGATCCTTGGCGTCTGACCAGAACGGCGACAG
AACACATAGCGGAGACAAAC

Downstream 100 bases:

>100_bases
CGGCAAACCTGAACGCCCCGCATTCGTGCGGGGCGTTGTCATTTGGAGGAGACGACATGGCAGAGAACGAAAGGGCAGAG
GCCACACGATCGTACGAGTT

Product: acyl-CoA dehydrogenase domain-containing protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MDFSFTDEQKQLADAVRRFIEKDYGFEARNKVVYSPEGVSQSHWDALAELGLTALPVPEAQGGFDGRAMDLLVVMQELGR
GLVVEPYQATVLGAQALKLAGGQEGLLEEVAGGAVKLAIAFGERQSRYELFNVTTRAAQQGSGWTLTGEKAVVVHGAQAD
KLIVSARTGGEARDTSGLSLFVVDRDAAGVTVKDYRTIDNLHAADIRFDNAPATLLGKAGEAWETIDAVADLGCVLLCAE
AIGLIDALNAATLEYTKTRQQFGVPIARFQALQHRMVDMFIHGEQARSITYLAAAHFEDGDVEARRRYVSAAKARVGQAA
RDVGQEAVQLHGGMGVTNELPAAHMFKRLTMINTTLGDVDHHLARFTSLPGFQQAA

Sequences:

>Translated_376_residues
MDFSFTDEQKQLADAVRRFIEKDYGFEARNKVVYSPEGVSQSHWDALAELGLTALPVPEAQGGFDGRAMDLLVVMQELGR
GLVVEPYQATVLGAQALKLAGGQEGLLEEVAGGAVKLAIAFGERQSRYELFNVTTRAAQQGSGWTLTGEKAVVVHGAQAD
KLIVSARTGGEARDTSGLSLFVVDRDAAGVTVKDYRTIDNLHAADIRFDNAPATLLGKAGEAWETIDAVADLGCVLLCAE
AIGLIDALNAATLEYTKTRQQFGVPIARFQALQHRMVDMFIHGEQARSITYLAAAHFEDGDVEARRRYVSAAKARVGQAA
RDVGQEAVQLHGGMGVTNELPAAHMFKRLTMINTTLGDVDHHLARFTSLPGFQQAA
>Mature_376_residues
MDFSFTDEQKQLADAVRRFIEKDYGFEARNKVVYSPEGVSQSHWDALAELGLTALPVPEAQGGFDGRAMDLLVVMQELGR
GLVVEPYQATVLGAQALKLAGGQEGLLEEVAGGAVKLAIAFGERQSRYELFNVTTRAAQQGSGWTLTGEKAVVVHGAQAD
KLIVSARTGGEARDTSGLSLFVVDRDAAGVTVKDYRTIDNLHAADIRFDNAPATLLGKAGEAWETIDAVADLGCVLLCAE
AIGLIDALNAATLEYTKTRQQFGVPIARFQALQHRMVDMFIHGEQARSITYLAAAHFEDGDVEARRRYVSAAKARVGQAA
RDVGQEAVQLHGGMGVTNELPAAHMFKRLTMINTTLGDVDHHLARFTSLPGFQQAA

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI226958412, Length=358, Percent_Identity=24.0223463687151, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI4557233, Length=317, Percent_Identity=25.8675078864353, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI226958414, Length=322, Percent_Identity=25.1552795031056, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI21361497, Length=399, Percent_Identity=24.812030075188, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI76496475, Length=385, Percent_Identity=25.974025974026, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4557235, Length=385, Percent_Identity=25.974025974026, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI4501859, Length=360, Percent_Identity=23.8888888888889, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI4501857, Length=367, Percent_Identity=25.6130790190736, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4557231, Length=367, Percent_Identity=24.5231607629428, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI187960098, Length=367, Percent_Identity=24.5231607629428, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786223, Length=377, Percent_Identity=23.8726790450928, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081958, Length=370, Percent_Identity=20.8108108108108, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17538396, Length=359, Percent_Identity=24.5125348189415, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17508101, Length=360, Percent_Identity=25.2777777777778, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71985184, Length=394, Percent_Identity=27.6649746192893, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17534899, Length=371, Percent_Identity=25.6064690026954, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17506239, Length=358, Percent_Identity=24.8603351955307, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17569725, Length=368, Percent_Identity=25, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17570075, Length=364, Percent_Identity=24.1758241758242, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71985192, Length=319, Percent_Identity=28.2131661442006, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71990804, Length=263, Percent_Identity=24.3346007604563, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24660351, Length=368, Percent_Identity=27.445652173913, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21355753, Length=360, Percent_Identity=24.7222222222222, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356377, Length=320, Percent_Identity=23.75, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24666513, Length=369, Percent_Identity=24.1192411924119, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24646207, Length=369, Percent_Identity=24.390243902439, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363737, Length=400, Percent_Identity=25, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 40484; Mature: 40484

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSFTDEQKQLADAVRRFIEKDYGFEARNKVVYSPEGVSQSHWDALAELGLTALPVPEA
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCCEECCCCCC
QGGFDGRAMDLLVVMQELGRGLVVEPYQATVLGAQALKLAGGQEGLLEEVAGGAVKLAIA
CCCCCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEEE
FGERQSRYELFNVTTRAAQQGSGWTLTGEKAVVVHGAQADKLIVSARTGGEARDTSGLSL
ECCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCEEEEEECCCCCCCCCCCCEE
FVVDRDAAGVTVKDYRTIDNLHAADIRFDNAPATLLGKAGEAWETIDAVADLGCVLLCAE
EEEECCCCCCCHHHHHHHCCCEECCEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AIGLIDALNAATLEYTKTRQQFGVPIARFQALQHRMVDMFIHGEQARSITYLAAAHFEDG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCC
DVEARRRYVSAAKARVGQAARDVGQEAVQLHGGMGVTNELPAAHMFKRLTMINTTLGDVD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
HHLARFTSLPGFQQAA
HHHHHHHCCCCHHCCC
>Mature Secondary Structure
MDFSFTDEQKQLADAVRRFIEKDYGFEARNKVVYSPEGVSQSHWDALAELGLTALPVPEA
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCCEECCCCCC
QGGFDGRAMDLLVVMQELGRGLVVEPYQATVLGAQALKLAGGQEGLLEEVAGGAVKLAIA
CCCCCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEEE
FGERQSRYELFNVTTRAAQQGSGWTLTGEKAVVVHGAQADKLIVSARTGGEARDTSGLSL
ECCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCEEEEEECCCCCCCCCCCCEE
FVVDRDAAGVTVKDYRTIDNLHAADIRFDNAPATLLGKAGEAWETIDAVADLGCVLLCAE
EEEECCCCCCCHHHHHHHCCCEECCEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AIGLIDALNAATLEYTKTRQQFGVPIARFQALQHRMVDMFIHGEQARSITYLAAAHFEDG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCC
DVEARRRYVSAAKARVGQAARDVGQEAVQLHGGMGVTNELPAAHMFKRLTMINTTLGDVD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
HHLARFTSLPGFQQAA
HHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]