BacMap

Definition	Ralstonia pickettii 12J chromosome chromosome 1, complete sequence.
Accession	NC_010682
Length	3,942,557

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The map label for this gene is braF [H]

Identifier: 187929269

GI number: 187929269

Start: 2299463

End: 2300314

Strand: Reverse

Name: braF [H]

Synonym: Rpic_2190

Alternate gene names: 187929269

Gene position: 2300314-2299463 (Counterclockwise)

Preceding gene: 187929270

Following gene: 187929268

Centisome position: 58.35

GC content: 65.14

Gene sequence:

>852_bases
ATGAGCACCTTCACCGAACTCCCCGACCGCGCGGAGACCGGCACCGCCACCGGCATGGGCCATCTGGTCGAGCCGGACAC
CATCGACGTCTCGCACGGCGCCATCCTGTATCTCGAAGATGTGACGGTGCGCTTTGGCGGCTTTCGCGCACTGAACGCGC
TCACGCTGTCGATCGACCACGGCGAACTGCGCTGCATCATCGGGCCCAACGGCGCGGGCAAGACCACGATGATGGACGTG
ATTACTGGCAAGACGGGGCCACGCAACGCCGACGTGACCGGCCGTGTCTTCCTCGGCCAGACCATCGACCTGCTGCGCCT
GACCGAGCCGCGCATCGCGCAGATCGGCATCGGCCGCAAGTTCCAGAAGCCGACCGTGTTCGAGCAGCACACCGTGTGGG
AAAACCTGGAACTGGCCATGAAAGCCGACAAGCGCTGGTGGGTCTCGCTGCGCGCGCGCCTGCTGAACGACGGCCGCCAG
CGCATCGAAGAAACGCTCGGTCGCATCCATCTGGAAGCGGAGGCCTATCGGCCCGCCGGGCTGCTCTCGCACGGCCAGAA
GCAGCGGCTGGAGATCGGCATGCTGCTGATGCAGCAACCGCAACTTCTGCTGCTCGATGAGCCCGTTGCCGGCATGACGG
ACGAAGAAACGATGCAGCTCGCAGCGTTGCTCAATGGCCTGCGCGGCACGTGCTCCATCATGGTCGTGGAGCACGACATG
GAGTTCGTCGCTGCGCTGGCAGGGGCAGAAGGACGCGTGACTGTGCTGGCCGAAGGCAGCGTCTTGGCCGAAGGCGCGCT
CGATGCCGTCAAGCGCGATGAGCGTGTGATCGAATCCTATCTGGGGCGTTGA

Upstream 100 bases:

>100_bases
CGAACGGCGTGCTGGGGCTGCTGGGCAAGCTGCGGATGAAGCGGCGCACGCCCACGCAAGCCAAGGCGCATGAAACCGCG
ACCGTCACCGGAGACCACGC

Downstream 100 bases:

>100_bases
TATGTTGCAGGTCCAACAACTGAACCAGTTCTACGGCGGCAGCCACATCCTGCGCAACGTCAGCTTTGACGTCCCCACGG
GCAAGCTGACCACGCTGCTT

Product: urea ABC transporter ATP-binding protein UrtD

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSTFTELPDRAETGTATGMGHLVEPDTIDVSHGAILYLEDVTVRFGGFRALNALTLSIDHGELRCIIGPNGAGKTTMMDV
ITGKTGPRNADVTGRVFLGQTIDLLRLTEPRIAQIGIGRKFQKPTVFEQHTVWENLELAMKADKRWWVSLRARLLNDGRQ
RIEETLGRIHLEAEAYRPAGLLSHGQKQRLEIGMLLMQQPQLLLLDEPVAGMTDEETMQLAALLNGLRGTCSIMVVEHDM
EFVAALAGAEGRVTVLAEGSVLAEGALDAVKRDERVIESYLGR

Sequences:

>Translated_283_residues
MSTFTELPDRAETGTATGMGHLVEPDTIDVSHGAILYLEDVTVRFGGFRALNALTLSIDHGELRCIIGPNGAGKTTMMDV
ITGKTGPRNADVTGRVFLGQTIDLLRLTEPRIAQIGIGRKFQKPTVFEQHTVWENLELAMKADKRWWVSLRARLLNDGRQ
RIEETLGRIHLEAEAYRPAGLLSHGQKQRLEIGMLLMQQPQLLLLDEPVAGMTDEETMQLAALLNGLRGTCSIMVVEHDM
EFVAALAGAEGRVTVLAEGSVLAEGALDAVKRDERVIESYLGR
>Mature_282_residues
STFTELPDRAETGTATGMGHLVEPDTIDVSHGAILYLEDVTVRFGGFRALNALTLSIDHGELRCIIGPNGAGKTTMMDVI
TGKTGPRNADVTGRVFLGQTIDLLRLTEPRIAQIGIGRKFQKPTVFEQHTVWENLELAMKADKRWWVSLRARLLNDGRQR
IEETLGRIHLEAEAYRPAGLLSHGQKQRLEIGMLLMQQPQLLLLDEPVAGMTDEETMQLAALLNGLRGTCSIMVVEHDME
FVAALAGAEGRVTVLAEGSVLAEGALDAVKRDERVIESYLGR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4674

COG function: function code R; Uncharacterized ABC-type transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=250, Percent_Identity=29.2, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI150417984, Length=248, Percent_Identity=30.241935483871, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI27881501, Length=228, Percent_Identity=25, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI30795238, Length=228, Percent_Identity=25, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI27436953, Length=227, Percent_Identity=27.3127753303965, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI45446740, Length=230, Percent_Identity=27.3913043478261, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI47078218, Length=230, Percent_Identity=27.3913043478261, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI46592915, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI46592898, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI46592971, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI46592956, Length=219, Percent_Identity=28.310502283105, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI46592964, Length=219, Percent_Identity=28.310502283105, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI46592978, Length=219, Percent_Identity=28.310502283105, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI153792144, Length=167, Percent_Identity=28.1437125748503, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI27262624, Length=163, Percent_Identity=29.4478527607362, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI27262626, Length=163, Percent_Identity=29.4478527607362, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI27477115, Length=168, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1789864, Length=258, Percent_Identity=34.8837209302326, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1790525, Length=248, Percent_Identity=29.0322580645161, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790544, Length=241, Percent_Identity=27.8008298755187, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789593, Length=251, Percent_Identity=27.8884462151394, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081791, Length=235, Percent_Identity=27.6595744680851, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790190, Length=256, Percent_Identity=28.125, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI87082268, Length=256, Percent_Identity=32.03125, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790739, Length=240, Percent_Identity=27.0833333333333, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787089, Length=233, Percent_Identity=27.8969957081545, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87081709, Length=249, Percent_Identity=26.5060240963855, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788761, Length=249, Percent_Identity=26.5060240963855, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788897, Length=244, Percent_Identity=26.6393442622951, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788053, Length=177, Percent_Identity=31.638418079096, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787792, Length=252, Percent_Identity=25.7936507936508, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787370, Length=223, Percent_Identity=28.2511210762332, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789672, Length=242, Percent_Identity=26.8595041322314, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788225, Length=251, Percent_Identity=27.4900398406374, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786872, Length=244, Percent_Identity=27.0491803278689, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790467, Length=234, Percent_Identity=27.3504273504274, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789991, Length=216, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787029, Length=238, Percent_Identity=24.7899159663866, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081782, Length=240, Percent_Identity=27.5, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081834, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788472, Length=250, Percent_Identity=24, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI48995001, Length=233, Percent_Identity=27.8969957081545, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790162, Length=219, Percent_Identity=25.5707762557078, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786398, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI48994943, Length=215, Percent_Identity=30.6976744186047, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788210, Length=216, Percent_Identity=27.3148148148148, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789873, Length=203, Percent_Identity=26.1083743842365, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786563, Length=233, Percent_Identity=27.4678111587983, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082267, Length=229, Percent_Identity=27.0742358078603, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786253, Length=235, Percent_Identity=24.2553191489362, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789586, Length=246, Percent_Identity=26.8292682926829, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787972, Length=254, Percent_Identity=26.7716535433071, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI2367384, Length=215, Percent_Identity=27.906976744186, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787105, Length=201, Percent_Identity=30.8457711442786, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787164, Length=239, Percent_Identity=26.3598326359833, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=239, Percent_Identity=28.4518828451883, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71983516, Length=243, Percent_Identity=29.6296296296296, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983510, Length=243, Percent_Identity=29.6296296296296, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=243, Percent_Identity=29.6296296296296, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17565586, Length=216, Percent_Identity=29.6296296296296, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115533608, Length=212, Percent_Identity=26.8867924528302, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24581191, Length=200, Percent_Identity=28, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581189, Length=200, Percent_Identity=28, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581193, Length=200, Percent_Identity=28, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581195, Length=200, Percent_Identity=28, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24666092, Length=252, Percent_Identity=23.4126984126984, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28574150, Length=219, Percent_Identity=26.4840182648402, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI116007328, Length=219, Percent_Identity=26.4840182648402, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI85816269, Length=210, Percent_Identity=27.1428571428571, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24580553, Length=210, Percent_Identity=27.1428571428571, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI85724864, Length=210, Percent_Identity=27.1428571428571, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24580555, Length=210, Percent_Identity=27.1428571428571, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 31081; Mature: 30950

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFTELPDRAETGTATGMGHLVEPDTIDVSHGAILYLEDVTVRFGGFRALNALTLSIDH
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEEEEEEECCEEEEEEEEEEECC
GELRCIIGPNGAGKTTMMDVITGKTGPRNADVTGRVFLGQTIDLLRLTEPRIAQIGIGRK
CEEEEEECCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCC
FQKPTVFEQHTVWENLELAMKADKRWWVSLRARLLNDGRQRIEETLGRIHLEAEAYRPAG
CCCCCCCCHHHHHCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCH
LLSHGQKQRLEIGMLLMQQPQLLLLDEPVAGMTDEETMQLAALLNGLRGTCSIMVVEHDM
HHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCH
EFVAALAGAEGRVTVLAEGSVLAEGALDAVKRDERVIESYLGR
HHHHHHCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STFTELPDRAETGTATGMGHLVEPDTIDVSHGAILYLEDVTVRFGGFRALNALTLSIDH
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEEEEEEECCEEEEEEEEEEECC
GELRCIIGPNGAGKTTMMDVITGKTGPRNADVTGRVFLGQTIDLLRLTEPRIAQIGIGRK
CEEEEEECCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCC
FQKPTVFEQHTVWENLELAMKADKRWWVSLRARLLNDGRQRIEETLGRIHLEAEAYRPAG
CCCCCCCCHHHHHCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCH
LLSHGQKQRLEIGMLLMQQPQLLLLDEPVAGMTDEETMQLAALLNGLRGTCSIMVVEHDM
HHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCH
EFVAALAGAEGRVTVLAEGSVLAEGALDAVKRDERVIESYLGR
HHHHHHCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]