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The map label for this gene is yfcG [H]

Identifier: 187929257

GI number: 187929257

Start: 2290703

End: 2291395

Strand: Direct

Name: yfcG [H]

Synonym: Rpic_2178

Alternate gene names: 187929257

Gene position: 2290703-2291395 (Clockwise)

Preceding gene: 187929256

Following gene: 187929258

Centisome position: 58.1

GC content: 61.33

Gene sequence:

>693_bases
ATGATCGACGTCTATAGCTGGGCCACGCCCAACGGGCACAAAGTGCACATCATGCTGGAAGAATGCGAGCTGCCTTACCG
CGTTCACGGCATCAACATCGGCGCCGGCGACCAGTTCAAGCCCGACTTCCTCAAGATCAGCCCCAACAACAAGATCCCGG
CCATCGTCGATCAGGATGGCCCCGACGGCAAACCGATCTCGCTGTTCGAATCGGGCGCGATCCTGCTGTACCTCGCCGGC
AAGACCGGCAAGTTCCTGCCCGACGACGTGCGTGGCAAATACGAAGTGCTGCAGTGGCTGATGTTCCAGATGGGCGGCGT
CGGCCCGATGCTCGGCCAGGCGCACCACTTCCGCATCTACGCGCCCGAGAAGATCGAATACGCGATCAACCGCTATACGA
ACGAAGCCAAGCGACTGTACGGCGTGATCGACAAGCGCCTGGGCGAATCCAAGTACCTCGGCGGCGCGGAGTACTCCATC
GCCGACATCGCCACGTGGCCGTGGCTGCGCAGTTGGAAGAACCAGGGGATCGAACTGTCGGACTACCCCAAGCTGCAGCG
CTGGTTTGAAGCCATTGAAGAACGCCCCGCCGTGCAGCGCGCGGTGAAAGTGCTGGCCAAGGAACGGCCGGCTGTGCAGG
ACGACAAGGCAAATGAAATCCTGTTTGGCGCCACGCAGTATCAGCGCCGTTGA

Upstream 100 bases:

>100_bases
GCGTCTGCGCGTTTCTCGGTTCTGTGCTGGAATGGAACACTTTGCAGGCTGCTGGTGGCCTGCACCTCTCTTCCCGGCCA
GAAGCCAAGGAGCCAACTCG

Downstream 100 bases:

>100_bases
GCCGCACACGCATCACGAGACAAAAGGCCGCGCATGCGGCCTTTTTAATGGCCGTTAGCAGGGCGCACCCCGCGCCGGTC
CTACACTGAGCGCATGCGGA

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MIDVYSWATPNGHKVHIMLEECELPYRVHGINIGAGDQFKPDFLKISPNNKIPAIVDQDGPDGKPISLFESGAILLYLAG
KTGKFLPDDVRGKYEVLQWLMFQMGGVGPMLGQAHHFRIYAPEKIEYAINRYTNEAKRLYGVIDKRLGESKYLGGAEYSI
ADIATWPWLRSWKNQGIELSDYPKLQRWFEAIEERPAVQRAVKVLAKERPAVQDDKANEILFGATQYQRR

Sequences:

>Translated_230_residues
MIDVYSWATPNGHKVHIMLEECELPYRVHGINIGAGDQFKPDFLKISPNNKIPAIVDQDGPDGKPISLFESGAILLYLAG
KTGKFLPDDVRGKYEVLQWLMFQMGGVGPMLGQAHHFRIYAPEKIEYAINRYTNEAKRLYGVIDKRLGESKYLGGAEYSI
ADIATWPWLRSWKNQGIELSDYPKLQRWFEAIEERPAVQRAVKVLAKERPAVQDDKANEILFGATQYQRR
>Mature_230_residues
MIDVYSWATPNGHKVHIMLEECELPYRVHGINIGAGDQFKPDFLKISPNNKIPAIVDQDGPDGKPISLFESGAILLYLAG
KTGKFLPDDVRGKYEVLQWLMFQMGGVGPMLGQAHHFRIYAPEKIEYAINRYTNEAKRLYGVIDKRLGESKYLGGAEYSI
ADIATWPWLRSWKNQGIELSDYPKLQRWFEAIEERPAVQRAVKVLAKERPAVQDDKANEILFGATQYQRR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=203, Percent_Identity=57.6354679802956, Blast_Score=241, Evalue=2e-65,
Organism=Escherichia coli, GI87082195, Length=215, Percent_Identity=47.4418604651163, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI87081789, Length=208, Percent_Identity=31.7307692307692, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324100, Length=235, Percent_Identity=32.7659574468085, Blast_Score=127, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28571670, Length=174, Percent_Identity=27.0114942528736, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17933730, Length=222, Percent_Identity=24.7747747747748, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17864598, Length=175, Percent_Identity=30.2857142857143, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17864592, Length=200, Percent_Identity=28, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646249, Length=163, Percent_Identity=28.8343558282209, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20130115, Length=198, Percent_Identity=26.2626262626263, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI85725204, Length=171, Percent_Identity=29.2397660818713, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17737923, Length=171, Percent_Identity=29.2397660818713, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17864594, Length=192, Percent_Identity=26.5625, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26239; Mature: 26239

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVYSWATPNGHKVHIMLEECELPYRVHGINIGAGDQFKPDFLKISPNNKIPAIVDQDG
CCCEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
PDGKPISLFESGAILLYLAGKTGKFLPDDVRGKYEVLQWLMFQMGGVGPMLGQAHHFRIY
CCCCCEEEECCCEEEEEEECCCCCCCCHHCCCHHHHHHHHHHHHCCCCCHHCCCCEEEEE
APEKIEYAINRYTNEAKRLYGVIDKRLGESKYLGGAEYSIADIATWPWLRSWKNQGIELS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DYPKLQRWFEAIEERPAVQRAVKVLAKERPAVQDDKANEILFGATQYQRR
CCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHHCCC
>Mature Secondary Structure
MIDVYSWATPNGHKVHIMLEECELPYRVHGINIGAGDQFKPDFLKISPNNKIPAIVDQDG
CCCEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
PDGKPISLFESGAILLYLAGKTGKFLPDDVRGKYEVLQWLMFQMGGVGPMLGQAHHFRIY
CCCCCEEEECCCEEEEEEECCCCCCCCHHCCCHHHHHHHHHHHHCCCCCHHCCCCEEEEE
APEKIEYAINRYTNEAKRLYGVIDKRLGESKYLGGAEYSIADIATWPWLRSWKNQGIELS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DYPKLQRWFEAIEERPAVQRAVKVLAKERPAVQDDKANEILFGATQYQRR
CCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]