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The map label for this gene is trpF

Identifier: 187929217

GI number: 187929217

Start: 2248721

End: 2249422

Strand: Reverse

Name: trpF

Synonym: Rpic_2138

Alternate gene names: 187929217

Gene position: 2249422-2248721 (Counterclockwise)

Preceding gene: 187929218

Following gene: 187929216

Centisome position: 57.05

GC content: 64.25

Gene sequence:

>702_bases
ATGCCGTTGCACCGTACCCGTATCAAGCTGTGTGGCCTGACCCAGCCTGCCGATGTCGATCACGCCGTCGCAATCGGCGC
CGATGCGATCGGGCTTGTTTTCTATCCACCCAGCCCGCGTTACGTCGCGATCGAACGTGCGGCAGAGCTGGCGCACCGCG
CCGGTCCGTTCGTCACCGTAACGGGCCTGTTCGTCAACGCCAGCGCCGATGATGTGGCGCGCGTGCTCGACCAAGTGCCG
CTGACGTTGCTTCAGTTCCACGGTGATGAGCCGGCAGAGCTGTGCGCCGAGATCGCCGGAAAGGTAGGGCTGCCCTGGTT
GCGCGCCCTGCGTGTTCAGCCCGGGGCCGATTTGGTAGAATTCGCGGATCGGTTTGCCACTGCCCAAGGCCTGCTGCTCG
ACGCATTTGTCGAGGGTTATGGCGGCGGCGGCCATGTTTTCGACTGGACCCTCATTCCTCCGCAATGGCTCCCGCAATCG
CCATCCTTGCCAACCACAAGCGCCGCTCCTCGGCTCGTTTTGAGTGGTGGGTTGAGCGCGCAAAACGTCGCTGGCGCGAT
TGAACGCGTGCGGCCATACGCTGTTGATGTCAGCAGCGGGATCGAGGCTGCACGGGGCGTGAAAGACCACGCCCGGATGA
CCGCATTTGTGCGTGCGGTCCGCGAGGCCGATGCTGCCCTGGGCGCATCGGTTCAAGCCTGA

Upstream 100 bases:

>100_bases
GCTATCCTGATGCTTTCTCGTTGCCGGCGTCTCCGGCATCGTCCAGCCTGTTTCGCGGCGTGTTCGACGAGCACGCCGGC
CTGTGAATCCCGCTTCCGCC

Downstream 100 bases:

>100_bases
GCCGATCTGGCTGGTCTCTCGCGACGCACGCCTTCCTGAATATCTCCGCTGACGGGTCTTCCATCCTGACCTCGGCGGAC
GCCTCGAGAGACTGCCATGT

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MPLHRTRIKLCGLTQPADVDHAVAIGADAIGLVFYPPSPRYVAIERAAELAHRAGPFVTVTGLFVNASADDVARVLDQVP
LTLLQFHGDEPAELCAEIAGKVGLPWLRALRVQPGADLVEFADRFATAQGLLLDAFVEGYGGGGHVFDWTLIPPQWLPQS
PSLPTTSAAPRLVLSGGLSAQNVAGAIERVRPYAVDVSSGIEAARGVKDHARMTAFVRAVREADAALGASVQA

Sequences:

>Translated_233_residues
MPLHRTRIKLCGLTQPADVDHAVAIGADAIGLVFYPPSPRYVAIERAAELAHRAGPFVTVTGLFVNASADDVARVLDQVP
LTLLQFHGDEPAELCAEIAGKVGLPWLRALRVQPGADLVEFADRFATAQGLLLDAFVEGYGGGGHVFDWTLIPPQWLPQS
PSLPTTSAAPRLVLSGGLSAQNVAGAIERVRPYAVDVSSGIEAARGVKDHARMTAFVRAVREADAALGASVQA
>Mature_232_residues
PLHRTRIKLCGLTQPADVDHAVAIGADAIGLVFYPPSPRYVAIERAAELAHRAGPFVTVTGLFVNASADDVARVLDQVPL
TLLQFHGDEPAELCAEIAGKVGLPWLRALRVQPGADLVEFADRFATAQGLLLDAFVEGYGGGGHVFDWTLIPPQWLPQSP
SLPTTSAAPRLVLSGGLSAQNVAGAIERVRPYAVDVSSGIEAARGVKDHARMTAFVRAVREADAALGASVQA

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=214, Percent_Identity=33.1775700934579, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320210, Length=222, Percent_Identity=25.2252252252252, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_RALPJ (B2U6Z0)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001899704.1
- ProteinModelPortal:   B2U6Z0
- SMR:   B2U6Z0
- GeneID:   6287814
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_2138
- HOGENOM:   HBG554803
- OMA:   SKICGIT
- ProtClustDB:   PRK01222
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 24558; Mature: 24427

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHRTRIKLCGLTQPADVDHAVAIGADAIGLVFYPPSPRYVAIERAAELAHRAGPFVTV
CCCCHHHHHEECCCCCCCCCHHEEECCCEEEEEEECCCCCEEHHHHHHHHHHHCCCEEEE
TGLFVNASADDVARVLDQVPLTLLQFHGDEPAELCAEIAGKVGLPWLRALRVQPGADLVE
EEEEEECCHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCHHHH
FADRFATAQGLLLDAFVEGYGGGGHVFDWTLIPPQWLPQSPSLPTTSAAPRLVLSGGLSA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCH
QNVAGAIERVRPYAVDVSSGIEAARGVKDHARMTAFVRAVREADAALGASVQA
HHHHHHHHHHCCEEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PLHRTRIKLCGLTQPADVDHAVAIGADAIGLVFYPPSPRYVAIERAAELAHRAGPFVTV
CCCHHHHHEECCCCCCCCCHHEEECCCEEEEEEECCCCCEEHHHHHHHHHHHCCCEEEE
TGLFVNASADDVARVLDQVPLTLLQFHGDEPAELCAEIAGKVGLPWLRALRVQPGADLVE
EEEEEECCHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCHHHH
FADRFATAQGLLLDAFVEGYGGGGHVFDWTLIPPQWLPQSPSLPTTSAAPRLVLSGGLSA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCH
QNVAGAIERVRPYAVDVSSGIEAARGVKDHARMTAFVRAVREADAALGASVQA
HHHHHHHHHHCCEEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA