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The map label for this gene is trpB [H]

Identifier: 187929216

GI number: 187929216

Start: 2247431

End: 2248624

Strand: Reverse

Name: trpB [H]

Synonym: Rpic_2137

Alternate gene names: 187929216

Gene position: 2248624-2247431 (Counterclockwise)

Preceding gene: 187929217

Following gene: 187929215

Centisome position: 57.03

GC content: 64.66

Gene sequence:

>1194_bases
ATGTACAACCTGCCCGACGCCCACGGCCATTTTGGCCCCTACGGCGGCACCTTCGTTGCGGAAACGCTGTCCCACGCGCT
GGATGAATTGCGCGACGCCTATGCGCGCTACCAGCACGATCCCGAGTTCATCAAGGAATACGAATACGAGCTGAAGCACT
TCGTCGGCCGTCCGTCGCCCATCTATCACGCACGCCGCCTGACCGAACACTGCGGCGGCGCGCAGATCTACCTCAAGCGC
GAAGACCTGAACCACACCGGCGCGCACAAGGTGAACAACGTGATTGGCCAGGCGCTGCTGGCACGCCGCATGGGCAAGCC
ACGCGTGATTGCGGAAACGGGGGCCGGCCAGCACGGCGTGGCCACCGCCACGATCGCGGCGCGTTACGGCATGGAGTGCG
TCGTCTACATGGGCAGCGAAGACGTGCGCCGCCAGGCCGCCAACGTCTACCGCATGAAGCTGCTGGGCGCGACCGTGGTG
CCCGTGGAGTCGGGCTCGCGCACGCTCAAGGACGCGCTGAACGAAGCGATGCGCGACTGGGTGACCAACGTGGCCGACAC
CTTCTACATCATCGGCACGGTGGCGGGCCCGCACCCGTATCCGATGATGGTGCGCGACTTCCAGGCCGTGATCGGCGAGG
AATGCAAGGTGCAGATGCCCGAGATGACCGGACGCCAGCCGGACGCCGTGATCGCCTGCGTGGGCGGCGGCTCCAACGCC
ATGGGCATCTTCTATCCGTACATCGATCATGCCGACGTCAAGCTGATCGGCGTGGAAGCAGCGGGCGAGGGCATCGAGAC
CGGCCGCCACGCCGCGTCGCTCATGGGGGGCTCACCGGGCGTGCTGCACGGTAACCGCACCTATCTGCTGCAGGATGAGG
ACGGCCAGATCATCGAGACGCATTCGATTTCGGCGGGGCTGGACTATCCGGGCGTCGGTCCAGAACACGCCTGGTTGAAG
GATGTGCAGCGCGCGGAATACGTCGGCATTACCGACAAGGAGGCGCTGCAGTCTTTCCATGACCTGTGCCGCATGGAGGG
CATCATCCCGGCGCTGGAATCGAGCCACGCACTGGCGTATGCGTGCAAGCTGGCGCCGACACTGCCCAAGGACAAGATCC
TGCTGGTGAACCTCTCGGGCCGAGGCGACAAGGATATGCATACTGTCGCTGAACTCTCCGGCATCCAACTCTAA

Upstream 100 bases:

>100_bases
CTGAGCCGATCTGGCTGGTCTCTCGCGACGCACGCCTTCCTGAATATCTCCGCTGACGGGTCTTCCATCCTGACCTCGGC
GGACGCCTCGAGAGACTGCC

Downstream 100 bases:

>100_bases
CGGCACTGGCACCCGCATGACCGAGCGCGCCATCGACGGCATCTTCAACGAGGACTGCATTACCGGGGTCGAGCATCTGG
CCGACGGCAGTGTGGACCTC

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MYNLPDAHGHFGPYGGTFVAETLSHALDELRDAYARYQHDPEFIKEYEYELKHFVGRPSPIYHARRLTEHCGGAQIYLKR
EDLNHTGAHKVNNVIGQALLARRMGKPRVIAETGAGQHGVATATIAARYGMECVVYMGSEDVRRQAANVYRMKLLGATVV
PVESGSRTLKDALNEAMRDWVTNVADTFYIIGTVAGPHPYPMMVRDFQAVIGEECKVQMPEMTGRQPDAVIACVGGGSNA
MGIFYPYIDHADVKLIGVEAAGEGIETGRHAASLMGGSPGVLHGNRTYLLQDEDGQIIETHSISAGLDYPGVGPEHAWLK
DVQRAEYVGITDKEALQSFHDLCRMEGIIPALESSHALAYACKLAPTLPKDKILLVNLSGRGDKDMHTVAELSGIQL

Sequences:

>Translated_397_residues
MYNLPDAHGHFGPYGGTFVAETLSHALDELRDAYARYQHDPEFIKEYEYELKHFVGRPSPIYHARRLTEHCGGAQIYLKR
EDLNHTGAHKVNNVIGQALLARRMGKPRVIAETGAGQHGVATATIAARYGMECVVYMGSEDVRRQAANVYRMKLLGATVV
PVESGSRTLKDALNEAMRDWVTNVADTFYIIGTVAGPHPYPMMVRDFQAVIGEECKVQMPEMTGRQPDAVIACVGGGSNA
MGIFYPYIDHADVKLIGVEAAGEGIETGRHAASLMGGSPGVLHGNRTYLLQDEDGQIIETHSISAGLDYPGVGPEHAWLK
DVQRAEYVGITDKEALQSFHDLCRMEGIIPALESSHALAYACKLAPTLPKDKILLVNLSGRGDKDMHTVAELSGIQL
>Mature_397_residues
MYNLPDAHGHFGPYGGTFVAETLSHALDELRDAYARYQHDPEFIKEYEYELKHFVGRPSPIYHARRLTEHCGGAQIYLKR
EDLNHTGAHKVNNVIGQALLARRMGKPRVIAETGAGQHGVATATIAARYGMECVVYMGSEDVRRQAANVYRMKLLGATVV
PVESGSRTLKDALNEAMRDWVTNVADTFYIIGTVAGPHPYPMMVRDFQAVIGEECKVQMPEMTGRQPDAVIACVGGGSNA
MGIFYPYIDHADVKLIGVEAAGEGIETGRHAASLMGGSPGVLHGNRTYLLQDEDGQIIETHSISAGLDYPGVGPEHAWLK
DVQRAEYVGITDKEALQSFHDLCRMEGIIPALESSHALAYACKLAPTLPKDKILLVNLSGRGDKDMHTVAELSGIQL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=384, Percent_Identity=55.9895833333333, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6321412, Length=383, Percent_Identity=56.9190600522193, Blast_Score=469, Evalue=1e-133,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 43353; Mature: 43353

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNLPDAHGHFGPYGGTFVAETLSHALDELRDAYARYQHDPEFIKEYEYELKHFVGRPSP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCH
IYHARRLTEHCGGAQIYLKREDLNHTGAHKVNNVIGQALLARRMGKPRVIAETGAGQHGV
HHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
ATATIAARYGMECVVYMGSEDVRRQAANVYRMKLLGATVVPVESGSRTLKDALNEAMRDW
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHH
VTNVADTFYIIGTVAGPHPYPMMVRDFQAVIGEECKVQMPEMTGRQPDAVIACVGGGSNA
HHHHHHHEEEEEECCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEECCCCCC
MGIFYPYIDHADVKLIGVEAAGEGIETGRHAASLMGGSPGVLHGNRTYLLQDEDGQIIET
EEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEECCEEEEEECCCCCEEEE
HSISAGLDYPGVGPEHAWLKDVQRAEYVGITDKEALQSFHDLCRMEGIIPALESSHALAY
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEEE
ACKLAPTLPKDKILLVNLSGRGDKDMHTVAELSGIQL
EEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MYNLPDAHGHFGPYGGTFVAETLSHALDELRDAYARYQHDPEFIKEYEYELKHFVGRPSP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCH
IYHARRLTEHCGGAQIYLKREDLNHTGAHKVNNVIGQALLARRMGKPRVIAETGAGQHGV
HHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
ATATIAARYGMECVVYMGSEDVRRQAANVYRMKLLGATVVPVESGSRTLKDALNEAMRDW
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHH
VTNVADTFYIIGTVAGPHPYPMMVRDFQAVIGEECKVQMPEMTGRQPDAVIACVGGGSNA
HHHHHHHEEEEEECCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEECCCCCC
MGIFYPYIDHADVKLIGVEAAGEGIETGRHAASLMGGSPGVLHGNRTYLLQDEDGQIIET
EEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEECCEEEEEECCCCCEEEE
HSISAGLDYPGVGPEHAWLKDVQRAEYVGITDKEALQSFHDLCRMEGIIPALESSHALAY
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEEE
ACKLAPTLPKDKILLVNLSGRGDKDMHTVAELSGIQL
EEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]