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The map label for this gene is hpaIM [H]

Identifier: 187929215

GI number: 187929215

Start: 2246593

End: 2247414

Strand: Reverse

Name: hpaIM [H]

Synonym: Rpic_2136

Alternate gene names: 187929215

Gene position: 2247414-2246593 (Counterclockwise)

Preceding gene: 187929216

Following gene: 187929214

Centisome position: 57.0

GC content: 64.6

Gene sequence:

>822_bases
ATGACCGAGCGCGCCATCGACGGCATCTTCAACGAGGACTGCATTACCGGGGTCGAGCATCTGGCCGACGGCAGTGTGGA
CCTCGTCATCGCCGATCCGCCGTACGGGCTGGGCAAGGATTACGGCAACGACTCCGACAAGCTGTCGGGCGATGCCTACC
TCGCCTGGTCCGAACGCTGGATCGATGCGGTGCGGCCGAAGCTGGCGCGCAACGGATCGCTCTACCTGTTCTGCACGTGG
CAATACGCGCCGGAACTGTTCGTGATGCTCAAGCGACGCCTCACGATGGTCAACGAGATCATCTGGGACCGCCGCGTCCC
CAGCATGGGCGGCAGCACGCGCAAGTTCTCGTCGGTGCACGACAACATCGGTTTTTTTGCCGCTTCACGCGATTACTACT
TCGATGTCGACGCCGTGCGCATTCCGTACGACGCCGAAACAAAGAAGGCGCGCAGCCGCAAGCGTTTCGAGGGCAAGAAG
TGGCTGGAAGTCGGCTACAACCCGAAGGACGTGTGGAGCGTGTCGCGCCTGCACCGGCAAGACCCGGAGCGCGCCGAGCA
TCCGACGCAGAAACCGCTGGAGCTGGTCGAACGCATGATCCTGGCAAGCTGCCCGCCGGGCGGCCTCGTGCTGGACCCGT
TTCTGGGCAGCGGCACCACGGCGGCCGCGTGTGCAAGGCTTGGCCGCCGCTTCGCCGGCTTCGAGATCAACGCCGACTAT
TGCCGCGTGGCACGTGAACGCGTGGCCGCTGTGCATGCCGCCGCTGCCACCAACGCCCAACCGATTGAATCGCAGGCGCC
TGAGACCATCAAGGTCGCCTGA

Upstream 100 bases:

>100_bases
GACAAGATCCTGCTGGTGAACCTCTCGGGCCGAGGCGACAAGGATATGCATACTGTCGCTGAACTCTCCGGCATCCAACT
CTAACGGCACTGGCACCCGC

Downstream 100 bases:

>100_bases
AACCTGATCCTCCTATGTCCCGCATCGCTCAAACCTTCTCACAACTGTCTGCGCAGGGCCGCAAGGGCCTGATTCCGTTC
ATCACGGCCGGCGATCCGTA

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.HpaI; Adenine-specific methyltransferase HpaI [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTERAIDGIFNEDCITGVEHLADGSVDLVIADPPYGLGKDYGNDSDKLSGDAYLAWSERWIDAVRPKLARNGSLYLFCTW
QYAPELFVMLKRRLTMVNEIIWDRRVPSMGGSTRKFSSVHDNIGFFAASRDYYFDVDAVRIPYDAETKKARSRKRFEGKK
WLEVGYNPKDVWSVSRLHRQDPERAEHPTQKPLELVERMILASCPPGGLVLDPFLGSGTTAAACARLGRRFAGFEINADY
CRVARERVAAVHAAAATNAQPIESQAPETIKVA

Sequences:

>Translated_273_residues
MTERAIDGIFNEDCITGVEHLADGSVDLVIADPPYGLGKDYGNDSDKLSGDAYLAWSERWIDAVRPKLARNGSLYLFCTW
QYAPELFVMLKRRLTMVNEIIWDRRVPSMGGSTRKFSSVHDNIGFFAASRDYYFDVDAVRIPYDAETKKARSRKRFEGKK
WLEVGYNPKDVWSVSRLHRQDPERAEHPTQKPLELVERMILASCPPGGLVLDPFLGSGTTAAACARLGRRFAGFEINADY
CRVARERVAAVHAAAATNAQPIESQAPETIKVA
>Mature_272_residues
TERAIDGIFNEDCITGVEHLADGSVDLVIADPPYGLGKDYGNDSDKLSGDAYLAWSERWIDAVRPKLARNGSLYLFCTWQ
YAPELFVMLKRRLTMVNEIIWDRRVPSMGGSTRKFSSVHDNIGFFAASRDYYFDVDAVRIPYDAETKKARSRKRFEGKKW
LEVGYNPKDVWSVSRLHRQDPERAEHPTQKPLELVERMILASCPPGGLVLDPFLGSGTTAAACARLGRRFAGFEINADYC
RVARERVAAVHAAAATNAQPIESQAPETIKVA

Specific function: This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=259, Percent_Identity=31.2741312741313, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 30576; Mature: 30445

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERAIDGIFNEDCITGVEHLADGSVDLVIADPPYGLGKDYGNDSDKLSGDAYLAWSERW
CCCHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHH
IDAVRPKLARNGSLYLFCTWQYAPELFVMLKRRLTMVNEIIWDRRVPSMGGSTRKFSSVH
HHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
DNIGFFAASRDYYFDVDAVRIPYDAETKKARSRKRFEGKKWLEVGYNPKDVWSVSRLHRQ
CCCCEEEECCCEEEECCEEECCCCCHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHC
DPERAEHPTQKPLELVERMILASCPPGGLVLDPFLGSGTTAAACARLGRRFAGFEINADY
CCHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCEEECHHH
CRVARERVAAVHAAAATNAQPIESQAPETIKVA
HHHHHHHHHHHHHHHCCCCCCCHHCCCCEEECC
>Mature Secondary Structure 
TERAIDGIFNEDCITGVEHLADGSVDLVIADPPYGLGKDYGNDSDKLSGDAYLAWSERW
CCHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHH
IDAVRPKLARNGSLYLFCTWQYAPELFVMLKRRLTMVNEIIWDRRVPSMGGSTRKFSSVH
HHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
DNIGFFAASRDYYFDVDAVRIPYDAETKKARSRKRFEGKKWLEVGYNPKDVWSVSRLHRQ
CCCCEEEECCCEEEECCEEECCCCCHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHC
DPERAEHPTQKPLELVERMILASCPPGGLVLDPFLGSGTTAAACARLGRRFAGFEINADY
CCHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCEEECHHH
CRVARERVAAVHAAAATNAQPIESQAPETIKVA
HHHHHHHHHHHHHHHCCCCCCCHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1542567 [H]