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The map label for this gene is murI

Identifier: 187929182

GI number: 187929182

Start: 2218450

End: 2219298

Strand: Direct

Name: murI

Synonym: Rpic_2103

Alternate gene names: 187929182

Gene position: 2218450-2219298 (Clockwise)

Preceding gene: 187929181

Following gene: 187929184

Centisome position: 56.27

GC content: 66.08

Gene sequence:

>849_bases
ATGACGACACGCGCGCAGGCACCGGTCGGGGTCTTTGATTCCGGCCTCGGAGGGCTCTCCGTCCTGCGCGCGATTCGCGC
CGAACTGCCGGCCGAATCGCTTCTCTACCTGGCGGATTCCCGCCATGCCCCGTACGGGGAAAAATCTCCCGAGTACATTG
CCGATCGCACGCTGCGTGTGTGCGAATGGCTGGTCGACCAGGGCTGCAAGGCACTGGTGATCGCGTGCAACACCGCCACA
GCGCAGGCCGTGCACGTGCTGCGCGAAAAGCTCGCGGTGCCGGTGATTGGCGTCGAGCCGGGCTTAAAGCCGGCAGTTGC
GACGTCGAAGAGCCGTGTGGTCGGCGTGCTCGCGACCGAAAGCACGCTGCGTAGCGAGAAATTTGCGCGCCTGCTGGCGG
GTGCCTCTGGCGATTGCAAGGTGCTGAGCCAGCCCGGCTACGGCCTCGTCCCTCTGATCGAACGCGGCGATACCCACTCG
CCCGCGGTGCTGGAACTGCTGCGCGCCTATCTGCAACCGATGCTCGATGCGAATGCCGACACGTTGGTGCTCGGCTGCAC
GCACTACCCCTTCCTGGAAGATGCCATCCATGAGATTGCGGGCGACCGGTTGACATTGATCGACACCGGACACGCCGTCG
CGCGCCATCTGGGCCGCACGCTCGCGGCAGCGGGATTGCAGGCATCGGGCCAAGCCGCAACGCCATGCTTCATGAGCACC
GGCGATGTGCTTCCGCTGCAAGCCATGGTGGCTGCGTTGCTCGGAGAGGCGCCAATGGCCCAGCGGGTCGACATTGGCGA
CGCGCCCCTGAGCCCCGTCGCGGTGTCGCTCGCTTCCCAGCCCGAATAA

Upstream 100 bases:

>100_bases
GCTCGGGCACGTCGGTCCACAAGACCGGCCCGGCGGAATGGCAAGCGAAGATCGGCAAGATTCCGGTCGCAGCGGGCTGA
TCCGTTCATTCACATTCAAT

Downstream 100 bases:

>100_bases
CGCCGCCCGAATAGGACAACGCCGCCCTGAAAGGCGGCGCATTGTCATCTTGGCTGAATGTCAGCCAACCGTCGGTTGCA
CTGCCGGTTCGGCAACGTCC

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTTRAQAPVGVFDSGLGGLSVLRAIRAELPAESLLYLADSRHAPYGEKSPEYIADRTLRVCEWLVDQGCKALVIACNTAT
AQAVHVLREKLAVPVIGVEPGLKPAVATSKSRVVGVLATESTLRSEKFARLLAGASGDCKVLSQPGYGLVPLIERGDTHS
PAVLELLRAYLQPMLDANADTLVLGCTHYPFLEDAIHEIAGDRLTLIDTGHAVARHLGRTLAAAGLQASGQAATPCFMST
GDVLPLQAMVAALLGEAPMAQRVDIGDAPLSPVAVSLASQPE

Sequences:

>Translated_282_residues
MTTRAQAPVGVFDSGLGGLSVLRAIRAELPAESLLYLADSRHAPYGEKSPEYIADRTLRVCEWLVDQGCKALVIACNTAT
AQAVHVLREKLAVPVIGVEPGLKPAVATSKSRVVGVLATESTLRSEKFARLLAGASGDCKVLSQPGYGLVPLIERGDTHS
PAVLELLRAYLQPMLDANADTLVLGCTHYPFLEDAIHEIAGDRLTLIDTGHAVARHLGRTLAAAGLQASGQAATPCFMST
GDVLPLQAMVAALLGEAPMAQRVDIGDAPLSPVAVSLASQPE
>Mature_281_residues
TTRAQAPVGVFDSGLGGLSVLRAIRAELPAESLLYLADSRHAPYGEKSPEYIADRTLRVCEWLVDQGCKALVIACNTATA
QAVHVLREKLAVPVIGVEPGLKPAVATSKSRVVGVLATESTLRSEKFARLLAGASGDCKVLSQPGYGLVPLIERGDTHSP
AVLELLRAYLQPMLDANADTLVLGCTHYPFLEDAIHEIAGDRLTLIDTGHAVARHLGRTLAAAGLQASGQAATPCFMSTG
DVLPLQAMVAALLGEAPMAQRVDIGDAPLSPVAVSLASQPE

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=210, Percent_Identity=37.1428571428571, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_RALPJ (B2U6V5)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001899669.1
- GeneID:   6288189
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_2103
- HOGENOM:   HBG645102
- OMA:   CGHIPYG
- ProtClustDB:   PRK00865
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 29514; Mature: 29383

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRAQAPVGVFDSGLGGLSVLRAIRAELPAESLLYLADSRHAPYGEKSPEYIADRTLRV
CCCCCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
CEWLVDQGCKALVIACNTATAQAVHVLREKLAVPVIGVEPGLKPAVATSKSRVVGVLATE
HHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCCCCHHHCCCCCEEEEEECH
STLRSEKFARLLAGASGDCKVLSQPGYGLVPLIERGDTHSPAVLELLRAYLQPMLDANAD
HHHHHHHHHHHHHCCCCCCEEECCCCCCEEEHCCCCCCCCHHHHHHHHHHHHHHHCCCCC
TLVLGCTHYPFLEDAIHEIAGDRLTLIDTGHAVARHLGRTLAAAGLQASGQAATPCFMST
EEEEECCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GDVLPLQAMVAALLGEAPMAQRVDIGDAPLSPVAVSLASQPE
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCC
>Mature Secondary Structure 
TTRAQAPVGVFDSGLGGLSVLRAIRAELPAESLLYLADSRHAPYGEKSPEYIADRTLRV
CCCCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
CEWLVDQGCKALVIACNTATAQAVHVLREKLAVPVIGVEPGLKPAVATSKSRVVGVLATE
HHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCCCCHHHCCCCCEEEEEECH
STLRSEKFARLLAGASGDCKVLSQPGYGLVPLIERGDTHSPAVLELLRAYLQPMLDANAD
HHHHHHHHHHHHHCCCCCCEEECCCCCCEEEHCCCCCCCCHHHHHHHHHHHHHHHCCCCC
TLVLGCTHYPFLEDAIHEIAGDRLTLIDTGHAVARHLGRTLAAAGLQASGQAATPCFMST
EEEEECCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GDVLPLQAMVAALLGEAPMAQRVDIGDAPLSPVAVSLASQPE
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA