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The map label for this gene is fumB [H]

Identifier: 187929181

GI number: 187929181

Start: 2216906

End: 2218429

Strand: Direct

Name: fumB [H]

Synonym: Rpic_2102

Alternate gene names: 187929181

Gene position: 2216906-2218429 (Clockwise)

Preceding gene: 187929180

Following gene: 187929182

Centisome position: 56.23

GC content: 64.37

Gene sequence:

>1524_bases
ATGACCGTCATTCGTCAAGAAGACCTCATCCAGAGCGTCGCCGACGCGCTGCAGTACATCAGCTACTACCACCCGATGGA
CTACATCACTGCTCTGGGCCGCGCCTATGAGCAGGAGCAGAGCCCGGCGGCCAAGGATGCCATCGCGCAGATCCTGACCA
ACAGCCGCATGTGCGCCGAAGGCAAGCGCCCGATCTGTCAGGACACCGGCATCGTCACCGTGTTCCTGAAGGTCGGCATG
AACGTGCGCTGGGACGGCGCCACGATGAGCCTGGAAGACATGGTCAACGAAGGCGTGCGCCGCGCGTACAACGACGTCGA
CAACAAGCTGCGTGCAAGCGTGCTGGCTGATCCCGCTGGCAAACGCACCAACACGAAGGACAACACCCCGGCCGTGATCA
ACATGTCGATCGTGCCGGGCGACACAGTGGACGTGATCGTCGCGGCCAAGGGCGGCGGCTCCGAAGCCAAGTCGAAGTTC
GTGATGCTGAATCCGTCGGATTCGATCGTCGACTGGGTGCTCAAGACCGTGCCGACCATGGGCGCCGGCTGGTGCCCGCC
GGGCATGCTGGGCATCGGCATTGGCGGCACGGCTGAAAAGGCCATGCTGCTGGCCAAGGAAGCCCTGATGGAGCCGATCG
ACATTCAAGACCTGATCGCCCGCGGCCCCAGCAACCGCGCAGAAGAACTGCGCATCGAGCTGTATGAGAAGGTCAACGCG
CTGGGCATTGGCGCGCAAGGCCTGGGCGGCCTGGCCACCGTGCTCGACGTGAAGATCCTGGACTACCCGACGCACGCAGC
CAACCTGCCGGTTGCCATGATCCCGAACTGCGCCGCCACGCGCCACGCGCACTTCACCCTGGACGGCAGCGGCGTTGCCA
AACTGGAAGCGCCGGACCTGTCGCAATGGCCGAAGGTCGAGTGGGCACCGAACACGGAAACGTCGAAGCGCGTCGACCTG
AATACGCTCACGCCGGAAGAAGTTGCCTCGTGGAAGCCCGGCCAGACGCTGCTGCTGAACGGCAAGATGCTGACGGGTCG
CGACGCCGCACACAAGCGCATCGCCGACATGCTCGCCAAGGGCGAAAAGCTGCCGATCGACTTCACCAACCGCGTGATCT
ATTACGTCGGCCCGGTCGACCCGGTGCGCGATGAGGTTGTCGGCCCGGCAGGCCCGACCACGGCCACGCGCATGGACAAG
TTCACCGAGACGATGCTCGCCCAGACGGGTCTGATCGCCATGGTGGGCAAGGCCGAGCGCGGCCCGGTCGCCATCGAGTC
CATCAAGAAGCACAAGTCGGCTTACTTGATGGCCGTGGGCGGCGCGGCGTACCTGGTGTCGAAGGCCATCCGCGGCTCGA
AGGTGCTGGCGTTCGAAGACCTTGGCATGGAAGCCATCTATGAGTTTGACGTGAAGGACATGCCTGTCACCGTGGCCGTG
GACAGCTCGGGCACGTCGGTCCACAAGACCGGCCCGGCGGAATGGCAAGCGAAGATCGGCAAGATTCCGGTCGCAGCGGG
CTGA

Upstream 100 bases:

>100_bases
GCCTGCGGCGCCCGCCGTCCAGGTCACAGGCAGGGAGTGAAGGTCACCGACCACTGCCCTGCCGCACCGGCTTTTCATTC
CTATACGTTGGTCCCCCACC

Downstream 100 bases:

>100_bases
TCCGTTCATTCACATTCAATATGACGACACGCGCGCAGGCACCGGTCGGGGTCTTTGATTCCGGCCTCGGAGGGCTCTCC
GTCCTGCGCGCGATTCGCGC

Product: hydro-lyase, Fe-S type, tartrate/fumarate subfamily subunit alpha

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTVIRQEDLIQSVADALQYISYYHPMDYITALGRAYEQEQSPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTVFLKVGM
NVRWDGATMSLEDMVNEGVRRAYNDVDNKLRASVLADPAGKRTNTKDNTPAVINMSIVPGDTVDVIVAAKGGGSEAKSKF
VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLIARGPSNRAEELRIELYEKVNA
LGIGAQGLGGLATVLDVKILDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDLSQWPKVEWAPNTETSKRVDL
NTLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAKGEKLPIDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDK
FTETMLAQTGLIAMVGKAERGPVAIESIKKHKSAYLMAVGGAAYLVSKAIRGSKVLAFEDLGMEAIYEFDVKDMPVTVAV
DSSGTSVHKTGPAEWQAKIGKIPVAAG

Sequences:

>Translated_507_residues
MTVIRQEDLIQSVADALQYISYYHPMDYITALGRAYEQEQSPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTVFLKVGM
NVRWDGATMSLEDMVNEGVRRAYNDVDNKLRASVLADPAGKRTNTKDNTPAVINMSIVPGDTVDVIVAAKGGGSEAKSKF
VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLIARGPSNRAEELRIELYEKVNA
LGIGAQGLGGLATVLDVKILDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDLSQWPKVEWAPNTETSKRVDL
NTLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAKGEKLPIDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDK
FTETMLAQTGLIAMVGKAERGPVAIESIKKHKSAYLMAVGGAAYLVSKAIRGSKVLAFEDLGMEAIYEFDVKDMPVTVAV
DSSGTSVHKTGPAEWQAKIGKIPVAAG
>Mature_506_residues
TVIRQEDLIQSVADALQYISYYHPMDYITALGRAYEQEQSPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTVFLKVGMN
VRWDGATMSLEDMVNEGVRRAYNDVDNKLRASVLADPAGKRTNTKDNTPAVINMSIVPGDTVDVIVAAKGGGSEAKSKFV
MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLIARGPSNRAEELRIELYEKVNAL
GIGAQGLGGLATVLDVKILDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDLSQWPKVEWAPNTETSKRVDLN
TLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAKGEKLPIDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKF
TETMLAQTGLIAMVGKAERGPVAIESIKKHKSAYLMAVGGAAYLVSKAIRGSKVLAFEDLGMEAIYEFDVKDMPVTVAVD
SSGTSVHKTGPAEWQAKIGKIPVAAG

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=467, Percent_Identity=31.9057815845824, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1790564, Length=470, Percent_Identity=30.8510638297872, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1789442, Length=289, Percent_Identity=29.4117647058824, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=27.9329608938547, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54341; Mature: 54210

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIRQEDLIQSVADALQYISYYHPMDYITALGRAYEQEQSPAAKDAIAQILTNSRMCAE
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHC
GKRPICQDTGIVTVFLKVGMNVRWDGATMSLEDMVNEGVRRAYNDVDNKLRASVLADPAG
CCCCCCCCCCCEEEEEHHCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KRTNTKDNTPAVINMSIVPGDTVDVIVAAKGGGSEAKSKFVMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLIARGPSNRAEELRIELYEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHH
LGIGAQGLGGLATVLDVKILDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDL
EECCCCCCCCHHHHHEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCCCEEEECCCCC
SQWPKVEWAPNTETSKRVDLNTLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAK
CCCCCCEECCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECEEECCCHHHHHHHHHHHHC
GEKLPIDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTETMLAQTGLIAMVGKAER
CCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
GPVAIESIKKHKSAYLMAVGGAAYLVSKAIRGSKVLAFEDLGMEAIYEFDVKDMPVTVAV
CCHHHHHHHHHCCEEEEEECHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCEEEEE
DSSGTSVHKTGPAEWQAKIGKIPVAAG
CCCCCEEECCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVIRQEDLIQSVADALQYISYYHPMDYITALGRAYEQEQSPAAKDAIAQILTNSRMCAE
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHC
GKRPICQDTGIVTVFLKVGMNVRWDGATMSLEDMVNEGVRRAYNDVDNKLRASVLADPAG
CCCCCCCCCCCEEEEEHHCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KRTNTKDNTPAVINMSIVPGDTVDVIVAAKGGGSEAKSKFVMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLIARGPSNRAEELRIELYEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHH
LGIGAQGLGGLATVLDVKILDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDL
EECCCCCCCCHHHHHEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCCCEEEECCCCC
SQWPKVEWAPNTETSKRVDLNTLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAK
CCCCCCEECCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECEEECCCHHHHHHHHHHHHC
GEKLPIDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTETMLAQTGLIAMVGKAER
CCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
GPVAIESIKKHKSAYLMAVGGAAYLVSKAIRGSKVLAFEDLGMEAIYEFDVKDMPVTVAV
CCHHHHHHHHHCCEEEEEECHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCEEEEE
DSSGTSVHKTGPAEWQAKIGKIPVAAG
CCCCCEEECCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]