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The map label for this gene is purU [H]

Identifier: 187929153

GI number: 187929153

Start: 2183555

End: 2184421

Strand: Reverse

Name: purU [H]

Synonym: Rpic_2074

Alternate gene names: 187929153

Gene position: 2184421-2183555 (Counterclockwise)

Preceding gene: 187929154

Following gene: 187929152

Centisome position: 55.41

GC content: 62.28

Gene sequence:

>867_bases
ATGAGTCACTCCGGTTTCATTCTTACCCTGTCGTGCCCGGACCAGCCTGGCATCGTCCACGCGGTGTCGGGCCTGCTGTT
TGAGCAAGGCTGCAACATTCTCGATTCGGACCAGTTCGGCGACGAATTCACCGGCCGTTTCTTCATGCGCGTGCATTTTG
TGCCGCAGGGGCAACCGCTTGATCTCGCTACGCTGCGCGACTGCTTTGCGCCCATCGGCGAACGTTTCTCCATGCAGTGG
GGCATGTTCGACGCCGCAGCCAAGCCGCGCGTGATGATTCTCGTCTCGAAGATCGGCCACTGCCTGAACGACCTGCTGTT
CCGTGCCCGCGCCGGCCAACTTCCGATCGAGATTGCGGCCATCGTCTCGAACCACCGCGATTTCTACCAACTGGCTGCGT
CGTACGACGTGCCGTTCATGCACCTGCCCTTGCTGCAGGCGACGGACGCGCAGAAGGCCCAGCAGGAAGCCCGCATCTGG
GAAATCGCGCAGGAGCAGCAGATCGACCTGGTGGTGCTCGCGCGCTATATGCAGATTCTTTCCGACAATCTGTGCCGCAA
GCTCGAAGGCCGCGCCATCAACATCCACCACTCGTTCCTGCCGAGCTTTAAGGGCGCCAAGCCGTATTACCAGGCGCACG
AGCGCGGTGTGAAGCTGATCGGCGCAACGGCCCATTACGTGACGGCCGACCTGGACGAAGGCCCGATCATCGAACAGGAA
ATCGAGCGCGTGGACCACAGCATGGACCCGGAGCAGCTCACCGCCGTCGGCCGCGACGTGGAGTGCGTGGCGCTGGCCCG
CGCCGTGAAGTGGCATGCGGAACACCGCATCCTGCTCAACGGCCACAAGACCGTCGTCTTCAAGTAA

Upstream 100 bases:

>100_bases
CGGTCGACGCCACGCTCGTTACACTCGACGCCCTTGCGCGCCTGAGTTCGGCCCTTGCGCCACAGGGGTTTGCGCCCGGC
AACACTATATAAAACGCTTC

Downstream 100 bases:

>100_bases
TCCAGTCAGAGTAGGGGAGCGCGATGCGACAGATCATTCTCGATACCGAAACCACCGGCCTGAATGCCGCCACGGGCGAC
CGCGTGATCGAAATCGGTTG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSHSGFILTLSCPDQPGIVHAVSGLLFEQGCNILDSDQFGDEFTGRFFMRVHFVPQGQPLDLATLRDCFAPIGERFSMQW
GMFDAAAKPRVMILVSKIGHCLNDLLFRARAGQLPIEIAAIVSNHRDFYQLAASYDVPFMHLPLLQATDAQKAQQEARIW
EIAQEQQIDLVVLARYMQILSDNLCRKLEGRAINIHHSFLPSFKGAKPYYQAHERGVKLIGATAHYVTADLDEGPIIEQE
IERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK

Sequences:

>Translated_288_residues
MSHSGFILTLSCPDQPGIVHAVSGLLFEQGCNILDSDQFGDEFTGRFFMRVHFVPQGQPLDLATLRDCFAPIGERFSMQW
GMFDAAAKPRVMILVSKIGHCLNDLLFRARAGQLPIEIAAIVSNHRDFYQLAASYDVPFMHLPLLQATDAQKAQQEARIW
EIAQEQQIDLVVLARYMQILSDNLCRKLEGRAINIHHSFLPSFKGAKPYYQAHERGVKLIGATAHYVTADLDEGPIIEQE
IERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK
>Mature_287_residues
SHSGFILTLSCPDQPGIVHAVSGLLFEQGCNILDSDQFGDEFTGRFFMRVHFVPQGQPLDLATLRDCFAPIGERFSMQWG
MFDAAAKPRVMILVSKIGHCLNDLLFRARAGQLPIEIAAIVSNHRDFYQLAASYDVPFMHLPLLQATDAQKAQQEARIWE
IAQEQQIDLVVLARYMQILSDNLCRKLEGRAINIHHSFLPSFKGAKPYYQAHERGVKLIGATAHYVTADLDEGPIIEQEI
ERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=153, Percent_Identity=33.9869281045752, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI209869995, Length=153, Percent_Identity=33.9869281045752, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI209869993, Length=153, Percent_Identity=33.9869281045752, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1787483, Length=281, Percent_Identity=38.7900355871886, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1788846, Length=192, Percent_Identity=28.6458333333333, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=189, Percent_Identity=30.1587301587302, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24582400, Length=196, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32413; Mature: 32282

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSGFILTLSCPDQPGIVHAVSGLLFEQGCNILDSDQFGDEFTGRFFMRVHFVPQGQPL
CCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCEEEEEEEECCCCCCC
DLATLRDCFAPIGERFSMQWGMFDAAAKPRVMILVSKIGHCLNDLLFRARAGQLPIEIAA
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEHHH
IVSNHRDFYQLAASYDVPFMHLPLLQATDAQKAQQEARIWEIAQEQQIDLVVLARYMQIL
HHHCCHHHHHHHHHCCCCEEEECCHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SDNLCRKLEGRAINIHHSFLPSFKGAKPYYQAHERGVKLIGATAHYVTADLDEGPIIEQE
HHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHHHHCCEEEEEEEEEEEEEECCCCCCHHHH
IERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK
HHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEC
>Mature Secondary Structure 
SHSGFILTLSCPDQPGIVHAVSGLLFEQGCNILDSDQFGDEFTGRFFMRVHFVPQGQPL
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCEEEEEEEECCCCCCC
DLATLRDCFAPIGERFSMQWGMFDAAAKPRVMILVSKIGHCLNDLLFRARAGQLPIEIAA
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEHHH
IVSNHRDFYQLAASYDVPFMHLPLLQATDAQKAQQEARIWEIAQEQQIDLVVLARYMQIL
HHHCCHHHHHHHHHCCCCEEEECCHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SDNLCRKLEGRAINIHHSFLPSFKGAKPYYQAHERGVKLIGATAHYVTADLDEGPIIEQE
HHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHHHHCCEEEEEEEEEEEEEECCCCCCHHHH
IERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK
HHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]