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The map label for this gene is yngG [H]

Identifier: 187929140

GI number: 187929140

Start: 2172312

End: 2173292

Strand: Reverse

Name: yngG [H]

Synonym: Rpic_2061

Alternate gene names: 187929140

Gene position: 2173292-2172312 (Counterclockwise)

Preceding gene: 187929142

Following gene: 187929139

Centisome position: 55.12

GC content: 67.58

Gene sequence:

>981_bases
ATGTCCCTCATGAACACCCCCGTGCCCACCACCGCTGTGATCCGCGAAGTCGGTTTACGAGACGGATTGCAAAGCATCCA
GACGATCCTGCCCACCGAGCACAAGCGCGCATGGGTGCAGGCCGCCCACGCTGCCGGTGTGCGCGAGATCGAGATCGGCT
CGTTCGTGCCCGCGCGCCTGCTGCCGCAACTGGCGGATACCGCGCAGCTTGTGGCCTACGCGCGCACGCTGCCAGAACTG
TTCGTCTCCGCACTGGTACCGAACCTGCGCGGTGCAGAGAACGCTGTCGCCAGCGGCGTGGACCTCATGATCGTGCCGTT
GTCGGCCAGCCATGCACACAGCCTCGCCAACCTGCGCAAGACACCAGACGAGGTGGTGGACGAGGTGGCGCGTATCCGTG
CGGCGCGCGACGCCGCCGGCGCCAAGACCTTGATCGAAGGCGGCGTCGGCACGGCGTTTGGTTGCACGCTACAGGGTGAG
GTCGAGGCCGATGAAGTCCTGCGCCTAATGCAGGCTCTCCTCGATGCCGGTGCCGATCGCGTCAGCCTGGCAGACACCGT
GGGCTATGCCGACCCCGCTGCCGTATCGCGCCTGTTCGAGCGCGCCGTTGCCATCGCCGGGGATCAGTTCTGGTGCGGCC
ACTTCCACGACACGCGGGGCCTGGGCCTGGCCAACGTCTATGCTGCCATGCAAGCGGGCGTGACGCGCTTTGACGCCTGC
CTGGGCGGCATCGGCGGCTGCCCGCACGCCCCAGGCGCGAGCGGCAACGTCACCACGGAAGACCTCGCCTTCATGCTGGA
GAGCATGGGCGTGGCCACCGGGCTCGACATTGGCCAGTTGCTGTCCCTGCGCGAACACCTGGCCGACTGGTTGCCCGGCG
AGACACTGCACGGCACGCTGTGGCGCGCCGGCCTGCCCAAGACCTTCCACCCGAATTCTCCCCAAGCGGCCCAATCTGCC
CAATCCGCCATCCTGGCTTGA

Upstream 100 bases:

>100_bases
AGGCTTTCCCGTCCGGAACGGAAGCGTGCGTACTTGATCGCTGTAGCTTCCGCGCAGAAAGGCATACAGTCGGAACATCG
ATTGACGCCTGCCTGACGCA

Downstream 100 bases:

>100_bases
CAAACATGCATCACGACGACGCTTCATCGCTGCCGCTCGCCGGCATCCGTGTGCTGGAATTCACGCACATGGTCATGGGC
CCCACCTGCGGGATGATCCT

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSLMNTPVPTTAVIREVGLRDGLQSIQTILPTEHKRAWVQAAHAAGVREIEIGSFVPARLLPQLADTAQLVAYARTLPEL
FVSALVPNLRGAENAVASGVDLMIVPLSASHAHSLANLRKTPDEVVDEVARIRAARDAAGAKTLIEGGVGTAFGCTLQGE
VEADEVLRLMQALLDAGADRVSLADTVGYADPAAVSRLFERAVAIAGDQFWCGHFHDTRGLGLANVYAAMQAGVTRFDAC
LGGIGGCPHAPGASGNVTTEDLAFMLESMGVATGLDIGQLLSLREHLADWLPGETLHGTLWRAGLPKTFHPNSPQAAQSA
QSAILA

Sequences:

>Translated_326_residues
MSLMNTPVPTTAVIREVGLRDGLQSIQTILPTEHKRAWVQAAHAAGVREIEIGSFVPARLLPQLADTAQLVAYARTLPEL
FVSALVPNLRGAENAVASGVDLMIVPLSASHAHSLANLRKTPDEVVDEVARIRAARDAAGAKTLIEGGVGTAFGCTLQGE
VEADEVLRLMQALLDAGADRVSLADTVGYADPAAVSRLFERAVAIAGDQFWCGHFHDTRGLGLANVYAAMQAGVTRFDAC
LGGIGGCPHAPGASGNVTTEDLAFMLESMGVATGLDIGQLLSLREHLADWLPGETLHGTLWRAGLPKTFHPNSPQAAQSA
QSAILA
>Mature_325_residues
SLMNTPVPTTAVIREVGLRDGLQSIQTILPTEHKRAWVQAAHAAGVREIEIGSFVPARLLPQLADTAQLVAYARTLPELF
VSALVPNLRGAENAVASGVDLMIVPLSASHAHSLANLRKTPDEVVDEVARIRAARDAAGAKTLIEGGVGTAFGCTLQGEV
EADEVLRLMQALLDAGADRVSLADTVGYADPAAVSRLFERAVAIAGDQFWCGHFHDTRGLGLANVYAAMQAGVTRFDACL
GGIGGCPHAPGASGNVTTEDLAFMLESMGVATGLDIGQLLSLREHLADWLPGETLHGTLWRAGLPKTFHPNSPQAAQSAQ
SAILA

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI109150422, Length=274, Percent_Identity=33.5766423357664, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI109150427, Length=274, Percent_Identity=33.5766423357664, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI62198232, Length=288, Percent_Identity=32.6388888888889, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI260654708, Length=111, Percent_Identity=38.7387387387387, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1786261, Length=290, Percent_Identity=25.5172413793103, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17510563, Length=287, Percent_Identity=33.7979094076655, Blast_Score=163, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24582381, Length=290, Percent_Identity=33.1034482758621, Blast_Score=172, Evalue=4e-43,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 34158; Mature: 34027

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLMNTPVPTTAVIREVGLRDGLQSIQTILPTEHKRAWVQAAHAAGVREIEIGSFVPARL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCHHHH
LPQLADTAQLVAYARTLPELFVSALVPNLRGAENAVASGVDLMIVPLSASHAHSLANLRK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHC
TPDEVVDEVARIRAARDAAGAKTLIEGGVGTAFGCTLQGEVEADEVLRLMQALLDAGADR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCC
VSLADTVGYADPAAVSRLFERAVAIAGDQFWCGHFHDTRGLGLANVYAAMQAGVTRFDAC
EEHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGGIGGCPHAPGASGNVTTEDLAFMLESMGVATGLDIGQLLSLREHLADWLPGETLHGTL
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
WRAGLPKTFHPNSPQAAQSAQSAILA
HHCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SLMNTPVPTTAVIREVGLRDGLQSIQTILPTEHKRAWVQAAHAAGVREIEIGSFVPARL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCHHHH
LPQLADTAQLVAYARTLPELFVSALVPNLRGAENAVASGVDLMIVPLSASHAHSLANLRK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHC
TPDEVVDEVARIRAARDAAGAKTLIEGGVGTAFGCTLQGEVEADEVLRLMQALLDAGADR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCC
VSLADTVGYADPAAVSRLFERAVAIAGDQFWCGHFHDTRGLGLANVYAAMQAGVTRFDAC
EEHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGGIGGCPHAPGASGNVTTEDLAFMLESMGVATGLDIGQLLSLREHLADWLPGETLHGTL
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
WRAGLPKTFHPNSPQAAQSAQSAILA
HHCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]