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The map label for this gene is gloB [H]

Identifier: 187929118

GI number: 187929118

Start: 2149608

End: 2150447

Strand: Reverse

Name: gloB [H]

Synonym: Rpic_2039

Alternate gene names: 187929118

Gene position: 2150447-2149608 (Counterclockwise)

Preceding gene: 187929121

Following gene: 187929117

Centisome position: 54.54

GC content: 64.29

Gene sequence:

>840_bases
ATGGTCCAGGGTGGTGTGCCGCGCGTCGTAGCGCTTGAGGTCGAGGCGATCGCTGCGTTTACCGACAATTATATTTGGGC
ACTGCACGATGGCCGTGTGGCTGCTGTCGTCGACCCGGGCGACGCGCAACCGGTGCTGCGCTTCTTGAAGGAACGCGGGC
TCGCGCTCGGTGCCATTGTAATCACACATCATCATGGCGATCACGTCGGCGGGGTGCGCGAACTGCTCGCGGCGTATCCG
GAGAATCCCGAGGGTGCGCCGCTGCAGGTCATCGGCCCAGCGGACGAATCGATCCCGTGCCGCACGCAGGCCGTGCGCGA
AGGCGACGTCGTGACGCTCACGCAGCCAGCCGCCACATTCCGGGTGATCGATGTGCCGGGTCATACCAGCGGTCACGTTG
CCTATGTAGGGCGCCTTCAGGGTAATGCCGCATCGGCGAGCCTGTTTTGTGGCGATACACTCTTCGCCACCGGTTGCGGC
CGGCTTTTTGAAGGGACGCCCGCGCAGATGCGCGCGTCGCTGGCCAAGCTGGCGGCACTGGCGCCCGATACGCGTGTCTA
TTGCGCGCATGAATACACGGCATCGAATGTGCGTTTTGCGCGTGCAGTCGAACCTGGCAACGCCGACTTGGCAGCCTGGG
AGGATGAGGTAGCGGCGTTGCGGGCCGAGGGCCGCTCGACTGTACCCACGACCGTTGGACATGAGCGGGCGACCAATCCC
TTCATGCGGTCGGATGACGCCAATGTGACGCAGGCCGTGGCACGCCATGCAGGTATCGACGCGGATGACCCCGTTGCCGT
ATTCGCCGCGTTGCGCCAGTGGAAGAACGATTTCCGTTGA

Upstream 100 bases:

>100_bases
ATCGAACTGGGCCTGCTCCCACCGCAGGACATATTGCCCGGGCGGCGAAGCGAGCCAGTGGTGCCAATCTATAATGCGAC
GATCGTTAGAGGAGTCGGAC

Downstream 100 bases:

>100_bases
GAACGCTCGGCGCGCGGCGATAACGCGGCCAGATGTGATGCATTGGGCAAGCGGCACGCCGGGTGAACGCGGGTTGACGC
GGTCGGATGCGTTTCTTACT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MVQGGVPRVVALEVEAIAAFTDNYIWALHDGRVAAVVDPGDAQPVLRFLKERGLALGAIVITHHHGDHVGGVRELLAAYP
ENPEGAPLQVIGPADESIPCRTQAVREGDVVTLTQPAATFRVIDVPGHTSGHVAYVGRLQGNAASASLFCGDTLFATGCG
RLFEGTPAQMRASLAKLAALAPDTRVYCAHEYTASNVRFARAVEPGNADLAAWEDEVAALRAEGRSTVPTTVGHERATNP
FMRSDDANVTQAVARHAGIDADDPVAVFAALRQWKNDFR

Sequences:

>Translated_279_residues
MVQGGVPRVVALEVEAIAAFTDNYIWALHDGRVAAVVDPGDAQPVLRFLKERGLALGAIVITHHHGDHVGGVRELLAAYP
ENPEGAPLQVIGPADESIPCRTQAVREGDVVTLTQPAATFRVIDVPGHTSGHVAYVGRLQGNAASASLFCGDTLFATGCG
RLFEGTPAQMRASLAKLAALAPDTRVYCAHEYTASNVRFARAVEPGNADLAAWEDEVAALRAEGRSTVPTTVGHERATNP
FMRSDDANVTQAVARHAGIDADDPVAVFAALRQWKNDFR
>Mature_279_residues
MVQGGVPRVVALEVEAIAAFTDNYIWALHDGRVAAVVDPGDAQPVLRFLKERGLALGAIVITHHHGDHVGGVRELLAAYP
ENPEGAPLQVIGPADESIPCRTQAVREGDVVTLTQPAATFRVIDVPGHTSGHVAYVGRLQGNAASASLFCGDTLFATGCG
RLFEGTPAQMRASLAKLAALAPDTRVYCAHEYTASNVRFARAVEPGNADLAAWEDEVAALRAEGRSTVPTTVGHERATNP
FMRSDDANVTQAVARHAGIDADDPVAVFAALRQWKNDFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=274, Percent_Identity=38.6861313868613, Blast_Score=175, Evalue=3e-44,
Organism=Homo sapiens, GI94538322, Length=274, Percent_Identity=38.6861313868613, Blast_Score=174, Evalue=5e-44,
Organism=Homo sapiens, GI116642887, Length=276, Percent_Identity=33.3333333333333, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI21703352, Length=276, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI14150041, Length=246, Percent_Identity=34.9593495934959, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=37.3737373737374, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1786406, Length=269, Percent_Identity=41.2639405204461, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787158, Length=255, Percent_Identity=27.843137254902, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=274, Percent_Identity=34.3065693430657, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320478, Length=269, Percent_Identity=31.2267657992565, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324614, Length=271, Percent_Identity=31.3653136531365, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356335, Length=275, Percent_Identity=38.1818181818182, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24667711, Length=275, Percent_Identity=38.1818181818182, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24667703, Length=271, Percent_Identity=38.7453874538745, Blast_Score=182, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29603; Mature: 29603

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQGGVPRVVALEVEAIAAFTDNYIWALHDGRVAAVVDPGDAQPVLRFLKERGLALGAIV
CCCCCCCEEEEEEHHEEEEECCCEEEEEECCEEEEEECCCCHHHHHHHHHHCCCEEEEEE
ITHHHGDHVGGVRELLAAYPENPEGAPLQVIGPADESIPCRTQAVREGDVVTLTQPAATF
EEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCEEEEECCCCEE
RVIDVPGHTSGHVAYVGRLQGNAASASLFCGDTLFATGCGRLFEGTPAQMRASLAKLAAL
EEEECCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHCCCHHHCCCHHHHHHHHHHHHHC
APDTRVYCAHEYTASNVRFARAVEPGNADLAAWEDEVAALRAEGRSTVPTTVGHERATNP
CCCCEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
FMRSDDANVTQAVARHAGIDADDPVAVFAALRQWKNDFR
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVQGGVPRVVALEVEAIAAFTDNYIWALHDGRVAAVVDPGDAQPVLRFLKERGLALGAIV
CCCCCCCEEEEEEHHEEEEECCCEEEEEECCEEEEEECCCCHHHHHHHHHHCCCEEEEEE
ITHHHGDHVGGVRELLAAYPENPEGAPLQVIGPADESIPCRTQAVREGDVVTLTQPAATF
EEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCEEEEECCCCEE
RVIDVPGHTSGHVAYVGRLQGNAASASLFCGDTLFATGCGRLFEGTPAQMRASLAKLAAL
EEEECCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHCCCHHHCCCHHHHHHHHHHHHHC
APDTRVYCAHEYTASNVRFARAVEPGNADLAAWEDEVAALRAEGRSTVPTTVGHERATNP
CCCCEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
FMRSDDANVTQAVARHAGIDADDPVAVFAALRQWKNDFR
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA