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The map label for this gene is mltD [H]

Identifier: 187929117

GI number: 187929117

Start: 2147919

End: 2149469

Strand: Reverse

Name: mltD [H]

Synonym: Rpic_2038

Alternate gene names: 187929117

Gene position: 2149469-2147919 (Counterclockwise)

Preceding gene: 187929118

Following gene: 187929116

Centisome position: 54.52

GC content: 64.73

Gene sequence:

>1551_bases
ATGCGATCCGTGCGACTTCTTGCGGCAACTGTGCTCAGTCTGCTCCTTGCGGCCTGTGCAACGGCCCCCGGGCCGAACGC
CGACACAGCCAGCACCAGCGCCTCCACCGCCACCGGCCAGGCCCCCGCGCCGGTCGTCAATGTCGATCAGCAGCCCGTGG
CATCCCTGAAAGGGCCTGCCAAGGATCTCTGGGCGCGCATTCGCCAAGGTTTCTCCATGCCCGACCTGCAAAGCTCGGCT
GTGGACGACCGCACTGACTGGTATGCGCAGCGCCCGGATGCCTTCCGCCGGATGGTGGATCGCTCGAACCGCTACCTGTA
CCACATCGTCGAAGAGCTGGAGCGGCGCAACATGCCCACCGAGCTGGCGCTCCTGCCGTTCGTGGAAAGTGCGTTCAACC
CGCAGGCAGTATCGACTGCCAAGGCTGCCGGGATGTGGCAGTTCATCCCGAGCACTGGCCGAACCTACAACCTCAAGCAG
AACGTCTTCCAGGACGAGCGCCGCGATGTGCTTGCCTCGACCGACGCCGCGCTCGACTATCTTTCCAAGCTGCACGACCA
GTTTGGCGACTGGCAACTCGCGCTGGCCGCCTACAACTGGGGTGAAGGCGCGGTGGCGCGGGCGATTGCACGCAACCAGG
CCGCGGGGCTGTCGACCGATTACGTCAACCTAAACATGCCCGCCGAGACGCGCATGTACGTGCCCAAGCTCCAGGCGGTG
AAGAACATCATCGCCAACCCGGAGCGCTACGGCGTCACGCTGCCGGAAATTCCCAACCATCCGTACTTCGTCACCGTCAC
GACTTCACGCGATATTGATGTGACGCTGGCTGCCAAGCTGGCCAATCTGCCAATGGATGAGTTCAAGGCGCTGAACCCGT
CGTTCAACCGGCCGGTGATCCTGGGCGCGTCGAACCCGCAGATCCTGCTGCCGTACGACAACGCGGAAACTTTCCAGTAC
AACCTGAACACCTATCGAGGCGGCCTCTCCAGCTGGACGGCCGTGACGGTCGGCAACCGCGAGCGCGTCGAAGCATTGGC
GGCCCGGCTGAAGGTTGACCCCGACACCATTCGCGAGATCAACCGCATTCCGAAGGGCATGCGGCTGAAGGCCGGCTCCA
CCGTGGTTGTGCCGCGTGCCGACGGCGCCCAGGAAAACGCTCCCGACATCAGCCCGGAGTTGGCCGAGAACGCCACCATG
GCGGTCGAACCCGACATGCCGGATCTGAGAAAGGTGGTCGTGCGCGCAGGCAAGCGCGATACGGTTGCGGGTCTGTCGCG
ACGCTACGGCGTGTCGGTTGCCCAGATTCGTGCGTGGAACCAGCTCCCCGGCGACGCCATTCCCAAGGGGCACAGCGTTG
TGCTGATGCTGCCGCAAGCGCACGGTGGCGGTCGTGGCGGGCATGTGCGCGTGATGCGCGTATCGGCCACAACGTCGCGT
GCCACAGCGGCAACCACGCGCGTGCCAACTGCGAAGGTGGCCGGCAAGCCGGTGGTCAAGGCGAAGCCGGTGGCAACTTC
ATCCAGTTCGGTCAAGCGCAAGAAGCGCTGA

Upstream 100 bases:

>100_bases
TGCATTGGGCAAGCGGCACGCCGGGTGAACGCGGGTTGACGCGGTCGGATGCGTTTCTTACTATCTGGGGCAATTTTTAG
CCCTAACCCCATAGAACTTG

Downstream 100 bases:

>100_bases
GACTGGCAACAGAAACGGCGTCCTGCGGGGCGCCGTTTTGTTTTGGGGCGGCGGTAAGACCCGCGTAAGTTGGCGATCGC
AAAGTAGGTGCATCAAGCAC

Product: MLTD_N domain-containing protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MRSVRLLAATVLSLLLAACATAPGPNADTASTSASTATGQAPAPVVNVDQQPVASLKGPAKDLWARIRQGFSMPDLQSSA
VDDRTDWYAQRPDAFRRMVDRSNRYLYHIVEELERRNMPTELALLPFVESAFNPQAVSTAKAAGMWQFIPSTGRTYNLKQ
NVFQDERRDVLASTDAALDYLSKLHDQFGDWQLALAAYNWGEGAVARAIARNQAAGLSTDYVNLNMPAETRMYVPKLQAV
KNIIANPERYGVTLPEIPNHPYFVTVTTSRDIDVTLAAKLANLPMDEFKALNPSFNRPVILGASNPQILLPYDNAETFQY
NLNTYRGGLSSWTAVTVGNRERVEALAARLKVDPDTIREINRIPKGMRLKAGSTVVVPRADGAQENAPDISPELAENATM
AVEPDMPDLRKVVVRAGKRDTVAGLSRRYGVSVAQIRAWNQLPGDAIPKGHSVVLMLPQAHGGGRGGHVRVMRVSATTSR
ATAATTRVPTAKVAGKPVVKAKPVATSSSSVKRKKR

Sequences:

>Translated_516_residues
MRSVRLLAATVLSLLLAACATAPGPNADTASTSASTATGQAPAPVVNVDQQPVASLKGPAKDLWARIRQGFSMPDLQSSA
VDDRTDWYAQRPDAFRRMVDRSNRYLYHIVEELERRNMPTELALLPFVESAFNPQAVSTAKAAGMWQFIPSTGRTYNLKQ
NVFQDERRDVLASTDAALDYLSKLHDQFGDWQLALAAYNWGEGAVARAIARNQAAGLSTDYVNLNMPAETRMYVPKLQAV
KNIIANPERYGVTLPEIPNHPYFVTVTTSRDIDVTLAAKLANLPMDEFKALNPSFNRPVILGASNPQILLPYDNAETFQY
NLNTYRGGLSSWTAVTVGNRERVEALAARLKVDPDTIREINRIPKGMRLKAGSTVVVPRADGAQENAPDISPELAENATM
AVEPDMPDLRKVVVRAGKRDTVAGLSRRYGVSVAQIRAWNQLPGDAIPKGHSVVLMLPQAHGGGRGGHVRVMRVSATTSR
ATAATTRVPTAKVAGKPVVKAKPVATSSSSVKRKKR
>Mature_516_residues
MRSVRLLAATVLSLLLAACATAPGPNADTASTSASTATGQAPAPVVNVDQQPVASLKGPAKDLWARIRQGFSMPDLQSSA
VDDRTDWYAQRPDAFRRMVDRSNRYLYHIVEELERRNMPTELALLPFVESAFNPQAVSTAKAAGMWQFIPSTGRTYNLKQ
NVFQDERRDVLASTDAALDYLSKLHDQFGDWQLALAAYNWGEGAVARAIARNQAAGLSTDYVNLNMPAETRMYVPKLQAV
KNIIANPERYGVTLPEIPNHPYFVTVTTSRDIDVTLAAKLANLPMDEFKALNPSFNRPVILGASNPQILLPYDNAETFQY
NLNTYRGGLSSWTAVTVGNRERVEALAARLKVDPDTIREINRIPKGMRLKAGSTVVVPRADGAQENAPDISPELAENATM
AVEPDMPDLRKVVVRAGKRDTVAGLSRRYGVSVAQIRAWNQLPGDAIPKGHSVVLMLPQAHGGGRGGHVRVMRVSATTSR
ATAATTRVPTAKVAGKPVVKAKPVATSSSSVKRKKR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=458, Percent_Identity=32.5327510917031, Blast_Score=224, Evalue=8e-60,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 56128; Mature: 56128

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVRLLAATVLSLLLAACATAPGPNADTASTSASTATGQAPAPVVNVDQQPVASLKGPA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHCCCH
KDLWARIRQGFSMPDLQSSAVDDRTDWYAQRPDAFRRMVDRSNRYLYHIVEELERRNMPT
HHHHHHHHCCCCCCCCCHHCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCH
ELALLPFVESAFNPQAVSTAKAAGMWQFIPSTGRTYNLKQNVFQDERRDVLASTDAALDY
HHHHHHHHHHCCCCHHHHHHHHCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
LSKLHDQFGDWQLALAAYNWGEGAVARAIARNQAAGLSTDYVNLNMPAETRMYVPKLQAV
HHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEECCCCHHCCHHHHHHHH
KNIIANPERYGVTLPEIPNHPYFVTVTTSRDIDVTLAAKLANLPMDEFKALNPSFNRPVI
HHHHCCCHHHCCCCCCCCCCCEEEEEECCCCCEEEEEHHHHHCCHHHHHCCCCCCCCCEE
LGASNPQILLPYDNAETFQYNLNTYRGGLSSWTAVTVGNRERVEALAARLKVDPDTIREI
EECCCCEEEEECCCCCEEEEECHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHH
NRIPKGMRLKAGSTVVVPRADGAQENAPDISPELAENATMAVEPDMPDLRKVVVRAGKRD
HHCCCCCEEECCCEEEEECCCCCCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHCCCCH
TVAGLSRRYGVSVAQIRAWNQLPGDAIPKGHSVVLMLPQAHGGGRGGHVRVMRVSATTSR
HHHHHHHHHCCCHHHEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCC
ATAATTRVPTAKVAGKPVVKAKPVATSSSSVKRKKR
HHHHHCCCCCHHHCCCCCEEECCCCCCCHHHHHCCC
>Mature Secondary Structure
MRSVRLLAATVLSLLLAACATAPGPNADTASTSASTATGQAPAPVVNVDQQPVASLKGPA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHCCCH
KDLWARIRQGFSMPDLQSSAVDDRTDWYAQRPDAFRRMVDRSNRYLYHIVEELERRNMPT
HHHHHHHHCCCCCCCCCHHCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCH
ELALLPFVESAFNPQAVSTAKAAGMWQFIPSTGRTYNLKQNVFQDERRDVLASTDAALDY
HHHHHHHHHHCCCCHHHHHHHHCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
LSKLHDQFGDWQLALAAYNWGEGAVARAIARNQAAGLSTDYVNLNMPAETRMYVPKLQAV
HHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEECCCCHHCCHHHHHHHH
KNIIANPERYGVTLPEIPNHPYFVTVTTSRDIDVTLAAKLANLPMDEFKALNPSFNRPVI
HHHHCCCHHHCCCCCCCCCCCEEEEEECCCCCEEEEEHHHHHCCHHHHHCCCCCCCCCEE
LGASNPQILLPYDNAETFQYNLNTYRGGLSSWTAVTVGNRERVEALAARLKVDPDTIREI
EECCCCEEEEECCCCCEEEEECHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHH
NRIPKGMRLKAGSTVVVPRADGAQENAPDISPELAENATMAVEPDMPDLRKVVVRAGKRD
HHCCCCCEEECCCEEEEECCCCCCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHCCCCH
TVAGLSRRYGVSVAQIRAWNQLPGDAIPKGHSVVLMLPQAHGGGRGGHVRVMRVSATTSR
HHHHHHHHHCCCHHHEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCC
ATAATTRVPTAKVAGKPVVKAKPVATSSSSVKRKKR
HHHHHCCCCCHHHCCCCCEEECCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]