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The map label for this gene is hpaG [H]

Identifier: 187929039

GI number: 187929039

Start: 2069492

End: 2070262

Strand: Reverse

Name: hpaG [H]

Synonym: Rpic_1959

Alternate gene names: 187929039

Gene position: 2070262-2069492 (Counterclockwise)

Preceding gene: 187929040

Following gene: 187929038

Centisome position: 52.51

GC content: 62.65

Gene sequence:

>771_bases
ATGAAACACGCGCGTATTGCCTATGACGGCGCGATCCATCACGCCACGGCCATGCCCGGCGACGACGCTCACCTGCGACT
GGCCGACGGCCGCGTCTTGCATGAAAGCGAAGTCGTCTGGTTGCCGCCGATCGAGCCGCGCACGGTTTTCGCACTGGGCC
TGAACTATGCCGACCACGCCAAGGAACTGGCCTTCAAGGCGCCCGAGGAGCCGCTCGCGTTCCTGAAAGGGCCGAATACG
CTGATCGGCCATCGCGCCCGCACCGTACGCCCGGATGGCGTGACGTTCATGCACTACGAATGCGAGCTGGTCATGGTGAT
TGGCAAGACCGCGCGCAATGTGCCGCGCGAGCATGCCTATGACTACGTGGCCGGATATACGGTGGCCAACGATTACGCTA
TCCGCGACTACCTCGAGAACTACTACCGCCCGAACCTGCGGGTGAAAAGCCGCGACACCTGTACGCCCATCGGGCCATGG
CTCGTTGACCGTGACGATGTGCCCGATCCGATGCACCTCGCGCTGCGCACCACCGTCAACGGCCGTGTCACGCAAGAAGG
CAGCACGCGCGACATGATCTTCGACATTCCCGCGTTGATCGCGTGGTTCTCGAGTTTCATGACGCTCAGCCCCGGCGACA
TGATCCTGACCGGCACGCCCGAGGGTTTGGCGGACACGCAACCCGGCGACGAAGTCATCACCGAAATTGAAGGCATCGGC
AGGCTGGTGAGCACCATCGTTGCCGAAGAATCGAACAAGGAGCCCGCATGA

Upstream 100 bases:

>100_bases
ATCCACCGCTGGCGCGGGCCGGTGATCGCATCGATATCGACATCGCCCACATTGGCACGCTGACGACGGCGTTGATTCGC
CACGCGCAGGAAGCTTCGCG

Downstream 100 bases:

>100_bases
GTCGCGTCAACATGGACCCTGTCAAGCATTGGATCAACGGTCGCCAGGTCGACAGCGTCGAGCGTTTCGTCACGACCAAT
CCGGCCACGGGCGAGGCCAT

Product: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase subunit HpaG2

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKHARIAYDGAIHHATAMPGDDAHLRLADGRVLHESEVVWLPPIEPRTVFALGLNYADHAKELAFKAPEEPLAFLKGPNT
LIGHRARTVRPDGVTFMHYECELVMVIGKTARNVPREHAYDYVAGYTVANDYAIRDYLENYYRPNLRVKSRDTCTPIGPW
LVDRDDVPDPMHLALRTTVNGRVTQEGSTRDMIFDIPALIAWFSSFMTLSPGDMILTGTPEGLADTQPGDEVITEIEGIG
RLVSTIVAEESNKEPA

Sequences:

>Translated_256_residues
MKHARIAYDGAIHHATAMPGDDAHLRLADGRVLHESEVVWLPPIEPRTVFALGLNYADHAKELAFKAPEEPLAFLKGPNT
LIGHRARTVRPDGVTFMHYECELVMVIGKTARNVPREHAYDYVAGYTVANDYAIRDYLENYYRPNLRVKSRDTCTPIGPW
LVDRDDVPDPMHLALRTTVNGRVTQEGSTRDMIFDIPALIAWFSSFMTLSPGDMILTGTPEGLADTQPGDEVITEIEGIG
RLVSTIVAEESNKEPA
>Mature_256_residues
MKHARIAYDGAIHHATAMPGDDAHLRLADGRVLHESEVVWLPPIEPRTVFALGLNYADHAKELAFKAPEEPLAFLKGPNT
LIGHRARTVRPDGVTFMHYECELVMVIGKTARNVPREHAYDYVAGYTVANDYAIRDYLENYYRPNLRVKSRDTCTPIGPW
LVDRDDVPDPMHLALRTTVNGRVTQEGSTRDMIFDIPALIAWFSSFMTLSPGDMILTGTPEGLADTQPGDEVITEIEGIG
RLVSTIVAEESNKEPA

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=225, Percent_Identity=40, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI40786394, Length=225, Percent_Identity=40, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI215422413, Length=201, Percent_Identity=35.8208955223881, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI66348062, Length=196, Percent_Identity=36.2244897959184, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI13654274, Length=196, Percent_Identity=36.2244897959184, Blast_Score=127, Evalue=8e-30,
Organism=Escherichia coli, GI1787428, Length=193, Percent_Identity=37.3056994818653, Blast_Score=132, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17557057, Length=187, Percent_Identity=38.5026737967914, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6324161, Length=225, Percent_Identity=28.8888888888889, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28572127, Length=227, Percent_Identity=41.8502202643172, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24663695, Length=219, Percent_Identity=34.703196347032, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28571789, Length=206, Percent_Identity=35.4368932038835, Blast_Score=118, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 28479; Mature: 28479

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHARIAYDGAIHHATAMPGDDAHLRLADGRVLHESEVVWLPPIEPRTVFALGLNYADHA
CCCCEEEECCCEEEEECCCCCCCEEEEECCEEEECCCEEEECCCCCCEEEEEECCCHHHH
KELAFKAPEEPLAFLKGPNTLIGHRARTVRPDGVTFMHYECELVMVIGKTARNVPREHAY
HHHHHCCCCCCHHHHCCCCHHHCCCCEEECCCCEEEEEEEEEEEEEECCHHHCCCHHHHH
DYVAGYTVANDYAIRDYLENYYRPNLRVKSRDTCTPIGPWLVDRDDVPDPMHLALRTTVN
HHHHCEEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCEECCCCCCCHHEEEEEEECC
GRVTQEGSTRDMIFDIPALIAWFSSFMTLSPGDMILTGTPEGLADTQPGDEVITEIEGIG
CEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
RLVSTIVAEESNKEPA
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKHARIAYDGAIHHATAMPGDDAHLRLADGRVLHESEVVWLPPIEPRTVFALGLNYADHA
CCCCEEEECCCEEEEECCCCCCCEEEEECCEEEECCCEEEECCCCCCEEEEEECCCHHHH
KELAFKAPEEPLAFLKGPNTLIGHRARTVRPDGVTFMHYECELVMVIGKTARNVPREHAY
HHHHHCCCCCCHHHHCCCCHHHCCCCEEECCCCEEEEEEEEEEEEEECCHHHCCCHHHHH
DYVAGYTVANDYAIRDYLENYYRPNLRVKSRDTCTPIGPWLVDRDDVPDPMHLALRTTVN
HHHHCEEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCEECCCCCCCHHEEEEEEECC
GRVTQEGSTRDMIFDIPALIAWFSSFMTLSPGDMILTGTPEGLADTQPGDEVITEIEGIG
CEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
RLVSTIVAEESNKEPA
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA