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The map label for this gene is hpaG [H]

Identifier: 187929040

GI number: 187929040

Start: 2070259

End: 2071011

Strand: Reverse

Name: hpaG [H]

Synonym: Rpic_1960

Alternate gene names: 187929040

Gene position: 2071011-2070259 (Counterclockwise)

Preceding gene: 187929041

Following gene: 187929039

Centisome position: 52.53

GC content: 63.88

Gene sequence:

>753_bases
ATGCGGCGAGCCAGAGTGACATTGCCGGACGGCCGGGTACGGGAAACCCTGTTTGCGTCTGCCACGGAACCCGCAGATTC
TTGGCGCTGGCTGCCGCCAGTCGAAGGCACGGTCGTCGGGGCCTTGCTCAACTACCGCGGAGAACTCGATGCGCTGAGTG
ACACGCTGTCTGCGCCGCCATACCAAGCGCCGCCCAAGGCGCCCATCCTGTATCTGAAGCCGGCCAACACGCGTATCGGC
CATGGTCACGAGGTTGCGCTGCCGGCAGACGTCGATGGCGTTTGGGCTGGAGCTTGCCTGGGCGTGGCGATCAGTTGTAC
TGCCACGCGTGTGCCTGCGCAGCACGCCGCGGATTTCATCGCCGGCTACACCATCGTCAACGACCTGACCGTGCCGCATG
CGAGCTACTACCGACCGGCGATCCGCCACAAGTGCCGCGATGGTTTTTGCCCGATGGGGCCCTGGATGGTCGACCGCGAC
GATGTTCCTAACGCCGATGCACTCGATATCACCGTGCGCATCAACGGCGAGGTCAAGCAGCGCGCAAATACGTCGACGCT
GGTGCGGCCAATCGCGCAGCTGCTTGCCGACGTGACGGCATTCATGACGCTTGAATCTGGCGATGTGCTGCTCGTTGGCA
TGCCGGAGAATCCACCGCTGGCGCGGGCCGGTGATCGCATCGATATCGACATCGCCCACATTGGCACGCTGACGACGGCG
TTGATTCGCCACGCGCAGGAAGCTTCGCGATGA

Upstream 100 bases:

>100_bases
GTGGCCGGCTTTTTCATTGCGCCGAAGTTTCCTGACTTGACATGGACGCAATCCGATTTGATTATTAACATGTTAATTAC
ATGAGAGAGGAGGAGGCGCA

Downstream 100 bases:

>100_bases
AACACGCGCGTATTGCCTATGACGGCGCGATCCATCACGCCACGGCCATGCCCGGCGACGACGCTCACCTGCGACTGGCC
GACGGCCGCGTCTTGCATGA

Product: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase subunit HpaG1

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MRRARVTLPDGRVRETLFASATEPADSWRWLPPVEGTVVGALLNYRGELDALSDTLSAPPYQAPPKAPILYLKPANTRIG
HGHEVALPADVDGVWAGACLGVAISCTATRVPAQHAADFIAGYTIVNDLTVPHASYYRPAIRHKCRDGFCPMGPWMVDRD
DVPNADALDITVRINGEVKQRANTSTLVRPIAQLLADVTAFMTLESGDVLLVGMPENPPLARAGDRIDIDIAHIGTLTTA
LIRHAQEASR

Sequences:

>Translated_250_residues
MRRARVTLPDGRVRETLFASATEPADSWRWLPPVEGTVVGALLNYRGELDALSDTLSAPPYQAPPKAPILYLKPANTRIG
HGHEVALPADVDGVWAGACLGVAISCTATRVPAQHAADFIAGYTIVNDLTVPHASYYRPAIRHKCRDGFCPMGPWMVDRD
DVPNADALDITVRINGEVKQRANTSTLVRPIAQLLADVTAFMTLESGDVLLVGMPENPPLARAGDRIDIDIAHIGTLTTA
LIRHAQEASR
>Mature_250_residues
MRRARVTLPDGRVRETLFASATEPADSWRWLPPVEGTVVGALLNYRGELDALSDTLSAPPYQAPPKAPILYLKPANTRIG
HGHEVALPADVDGVWAGACLGVAISCTATRVPAQHAADFIAGYTIVNDLTVPHASYYRPAIRHKCRDGFCPMGPWMVDRD
DVPNADALDITVRINGEVKQRANTSTLVRPIAQLLADVTAFMTLESGDVLLVGMPENPPLARAGDRIDIDIAHIGTLTTA
LIRHAQEASR

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=188, Percent_Identity=31.3829787234043, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI40786394, Length=188, Percent_Identity=31.3829787234043, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI13654274, Length=176, Percent_Identity=26.1363636363636, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI215422413, Length=180, Percent_Identity=25.5555555555556, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI66348062, Length=169, Percent_Identity=26.6272189349112, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1787428, Length=172, Percent_Identity=26.7441860465116, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17557057, Length=175, Percent_Identity=33.1428571428571, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28572127, Length=232, Percent_Identity=25, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571789, Length=179, Percent_Identity=28.4916201117318, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24663695, Length=223, Percent_Identity=24.6636771300448, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 26983; Mature: 26983

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRARVTLPDGRVRETLFASATEPADSWRWLPPVEGTVVGALLNYRGELDALSDTLSAPP
CCCCEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCC
YQAPPKAPILYLKPANTRIGHGHEVALPADVDGVWAGACLGVAISCTATRVPAQHAADFI
CCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHEEEEEEECCCCHHHHHHHH
AGYTIVNDLTVPHASYYRPAIRHKCRDGFCPMGPWMVDRDDVPNADALDITVRINGEVKQ
HHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEEEEEEEECCHHHH
RANTSTLVRPIAQLLADVTAFMTLESGDVLLVGMPENPPLARAGDRIDIDIAHIGTLTTA
CCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHH
LIRHAQEASR
HHHHHHHHCC
>Mature Secondary Structure
MRRARVTLPDGRVRETLFASATEPADSWRWLPPVEGTVVGALLNYRGELDALSDTLSAPP
CCCCEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCC
YQAPPKAPILYLKPANTRIGHGHEVALPADVDGVWAGACLGVAISCTATRVPAQHAADFI
CCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHEEEEEEECCCCHHHHHHHH
AGYTIVNDLTVPHASYYRPAIRHKCRDGFCPMGPWMVDRDDVPNADALDITVRINGEVKQ
HHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEEEEEEEECCHHHH
RANTSTLVRPIAQLLADVTAFMTLESGDVLLVGMPENPPLARAGDRIDIDIAHIGTLTTA
CCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHH
LIRHAQEASR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA