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The map label for this gene is xthA [H]

Identifier: 187929032

GI number: 187929032

Start: 2063767

End: 2064576

Strand: Direct

Name: xthA [H]

Synonym: Rpic_1952

Alternate gene names: 187929032

Gene position: 2063767-2064576 (Clockwise)

Preceding gene: 187929031

Following gene: 187929033

Centisome position: 52.35

GC content: 62.59

Gene sequence:

>810_bases
ATGCGTGTCGCCACCTGGAACGTCAACTCTTTGAAGGTCCGCCTACCGCACGTGCTGCAGTGGCTGGCTGAGCGCGAAGC
CGACGCCACGCCCATCGACCTGCTCTGCCTCCAAGAGCTCAAGCTGCCCGATGACCGCTATCCGCTCGCCGAACTGGACG
CAGCGGGCTATACGTCGTTGTTCACAGGCCAGAAGACCTACAACGGCGTTGCCATCCTCTCGCGCAAGGCCTCTGTGCCA
GAAGGCCGCGATGTGGTGAAGAACATCCCGGGCTTTGCCGACGAGCAGCAGCGCATCGTAGCCGCCACGTATGACGTGGA
CGGTGGCCCGGTCCGCGTGATTTCTGCCTACATCCCAAATGGACAAGCACTCGATTCCGACAAATTCGTCTACAAGCTGC
GCTGGCTGGAGGCACTGCAGGCATGGCTGACGAGCGAGTTGGCGGCAAACCCACGCCTGATGCTGCTGGGCGATTTCAAC
ATCGCTCCCGAAGATCGCGACGTGCACGACCCGAAAAAGTGGGAAGGCCAGAACCTGGTGTCTCCGGAGGAGCGGGCGGC
GTTTCGAGCGATGCAAGGCGCCGGGCTGGTCGATGCCTTCCGCATGTTCGAGCAGGAAGACAAGCTGTTCTCGTGGTGGG
ATTACCGCATGTTCGGCTTCAAGCGCAACGCCGGCCTGCGGATCGACCACATCATGCTGTCGCCTGAACTCGCCAAGCTG
TGCGAGTCGTGCCATATCGACCGCGTGCCACGCACGTGGGAGCAGCCTTCGGACCACACACCCGTGGTCGCCGCCCTGCG
CCGCGCATAA

Upstream 100 bases:

>100_bases
CGGCGTTTTCATTCGGGCCCGCAGCGCTTGATGGCACAATATCGCACCCCTGGACGGCGCCAAAGTGCGCGCCGTCGCCA
TTTGCAAGGACCGAGCCCTC

Downstream 100 bases:

>100_bases
GGAGTGCCCGGCATGGCTTCCGCCTTCAAGCACCGCAATCTTCCGCATCTCCTGCTGCGCGCGCGTGAGACCTTCATGGC
GCGCTTTCGGCCGATCCTGC

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MRVATWNVNSLKVRLPHVLQWLAEREADATPIDLLCLQELKLPDDRYPLAELDAAGYTSLFTGQKTYNGVAILSRKASVP
EGRDVVKNIPGFADEQQRIVAATYDVDGGPVRVISAYIPNGQALDSDKFVYKLRWLEALQAWLTSELAANPRLMLLGDFN
IAPEDRDVHDPKKWEGQNLVSPEERAAFRAMQGAGLVDAFRMFEQEDKLFSWWDYRMFGFKRNAGLRIDHIMLSPELAKL
CESCHIDRVPRTWEQPSDHTPVVAALRRA

Sequences:

>Translated_269_residues
MRVATWNVNSLKVRLPHVLQWLAEREADATPIDLLCLQELKLPDDRYPLAELDAAGYTSLFTGQKTYNGVAILSRKASVP
EGRDVVKNIPGFADEQQRIVAATYDVDGGPVRVISAYIPNGQALDSDKFVYKLRWLEALQAWLTSELAANPRLMLLGDFN
IAPEDRDVHDPKKWEGQNLVSPEERAAFRAMQGAGLVDAFRMFEQEDKLFSWWDYRMFGFKRNAGLRIDHIMLSPELAKL
CESCHIDRVPRTWEQPSDHTPVVAALRRA
>Mature_269_residues
MRVATWNVNSLKVRLPHVLQWLAEREADATPIDLLCLQELKLPDDRYPLAELDAAGYTSLFTGQKTYNGVAILSRKASVP
EGRDVVKNIPGFADEQQRIVAATYDVDGGPVRVISAYIPNGQALDSDKFVYKLRWLEALQAWLTSELAANPRLMLLGDFN
IAPEDRDVHDPKKWEGQNLVSPEERAAFRAMQGAGLVDAFRMFEQEDKLFSWWDYRMFGFKRNAGLRIDHIMLSPELAKL
CESCHIDRVPRTWEQPSDHTPVVAALRRA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=279, Percent_Identity=30.4659498207885, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI18375503, Length=279, Percent_Identity=30.4659498207885, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI18375501, Length=279, Percent_Identity=30.4659498207885, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1788046, Length=277, Percent_Identity=37.5451263537906, Blast_Score=164, Evalue=7e-42,
Organism=Drosophila melanogaster, GI221330655, Length=268, Percent_Identity=26.4925373134328, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17136678, Length=268, Percent_Identity=26.4925373134328, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30549; Mature: 30549

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVATWNVNSLKVRLPHVLQWLAEREADATPIDLLCLQELKLPDDRYPLAELDAAGYTSL
CEEEEECCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHCCCCCHHH
FTGQKTYNGVAILSRKASVPEGRDVVKNIPGFADEQQRIVAATYDVDGGPVRVISAYIPN
CCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEEECCC
GQALDSDKFVYKLRWLEALQAWLTSELAANPRLMLLGDFNIAPEDRDVHDPKKWEGQNLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
SPEERAAFRAMQGAGLVDAFRMFEQEDKLFSWWDYRMFGFKRNAGLRIDHIMLSPELAKL
CHHHHHHHHHHHCCCHHHHHHHHHHHCCHHEEEHHEEEEEECCCCCEEEEEEECHHHHHH
CESCHIDRVPRTWEQPSDHTPVVAALRRA
HHHCCCCCCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MRVATWNVNSLKVRLPHVLQWLAEREADATPIDLLCLQELKLPDDRYPLAELDAAGYTSL
CEEEEECCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHCCCCCHHH
FTGQKTYNGVAILSRKASVPEGRDVVKNIPGFADEQQRIVAATYDVDGGPVRVISAYIPN
CCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEEECCC
GQALDSDKFVYKLRWLEALQAWLTSELAANPRLMLLGDFNIAPEDRDVHDPKKWEGQNLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
SPEERAAFRAMQGAGLVDAFRMFEQEDKLFSWWDYRMFGFKRNAGLRIDHIMLSPELAKL
CHHHHHHHHHHHCCCHHHHHHHHHHHCCHHEEEHHEEEEEECCCCCEEEEEEECHHHHHH
CESCHIDRVPRTWEQPSDHTPVVAALRRA
HHHCCCCCCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]