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The map label for this gene is ispF

Identifier: 187928982

GI number: 187928982

Start: 2004026

End: 2004532

Strand: Reverse

Name: ispF

Synonym: Rpic_1902

Alternate gene names: 187928982

Gene position: 2004532-2004026 (Counterclockwise)

Preceding gene: 187928983

Following gene: 187928977

Centisome position: 50.84

GC content: 64.69

Gene sequence:

>507_bases
ATGAACTGGATGGACATCCGCATTGGTCAAGGCTATGACGTGCACGCGCTGGTGGAGGGCCGCAAGCTGATCCTGGGCGG
CGTGGAGATTCCGCACACACGCGGTCTGCTCGGCCACTCCGACGCCGATGCGCTGCTGCATGCGATTACCGATGCATTGT
TCGGCGCCGCCGGCCTGGGCGACATCGGCCGGCACTTTCCCGACACCGACCCGGCCTTTGCCGGCGCCGACAGCCGCGTG
CTGCTGCGCGAAGCCGTGCGCCGCGTGCACAAGGCCGGTTATGCGGTCGGCAATGTCGACGCCACCGTCATCGCGCAGAA
GCCCAAACTCGCACCACATGTGCCTGGCATGGTTGCCAACTTAGCGGAAGACCTCGGCATTGCGCCCGAGCGGTGCAACG
TCAAGGCCAAAACGAACGAGAAGCTCGGCTTCGAAGGCAAGGAGGAAGGCATCGTCGCGCAGGCGGTGGTGTTGATCTAT
CGCGCAGAAGCCGCCGAGCAGGACTAA

Upstream 100 bases:

>100_bases
CGGGGGCGCTGCGCAACTTCAAGGTGACGTATTCCGACGATTTCGCACTCGCCGAGGCCATCCTCGCGCAGCGCGCACAA
CCCAACGAGGCATCGCAAGC

Downstream 100 bases:

>100_bases
GTCCGCGTCGCCATCACGCGGAGGCAAGCGGCAGCACCGGAGCGATCCGGGCTGCCGTTTTCGTTTGCTGAACTGGTGCG
GCTATTCGGCCGCGATGACT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MNWMDIRIGQGYDVHALVEGRKLILGGVEIPHTRGLLGHSDADALLHAITDALFGAAGLGDIGRHFPDTDPAFAGADSRV
LLREAVRRVHKAGYAVGNVDATVIAQKPKLAPHVPGMVANLAEDLGIAPERCNVKAKTNEKLGFEGKEEGIVAQAVVLIY
RAEAAEQD

Sequences:

>Translated_168_residues
MNWMDIRIGQGYDVHALVEGRKLILGGVEIPHTRGLLGHSDADALLHAITDALFGAAGLGDIGRHFPDTDPAFAGADSRV
LLREAVRRVHKAGYAVGNVDATVIAQKPKLAPHVPGMVANLAEDLGIAPERCNVKAKTNEKLGFEGKEEGIVAQAVVLIY
RAEAAEQD
>Mature_168_residues
MNWMDIRIGQGYDVHALVEGRKLILGGVEIPHTRGLLGHSDADALLHAITDALFGAAGLGDIGRHFPDTDPAFAGADSRV
LLREAVRRVHKAGYAVGNVDATVIAQKPKLAPHVPGMVANLAEDLGIAPERCNVKAKTNEKLGFEGKEEGIVAQAVVLIY
RAEAAEQD

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=60.5095541401274, Blast_Score=185, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_RALPJ (B2UFT6)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001899469.1
- ProteinModelPortal:   B2UFT6
- SMR:   B2UFT6
- GeneID:   6287754
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1902
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17861; Mature: 17861

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWMDIRIGQGYDVHALVEGRKLILGGVEIPHTRGLLGHSDADALLHAITDALFGAAGLG
CCCEEEEECCCCCEEEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
DIGRHFPDTDPAFAGADSRVLLREAVRRVHKAGYAVGNVDATVIAQKPKLAPHVPGMVAN
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECCCCEEEEECCCCCCCCCCHHHHH
LAEDLGIAPERCNVKAKTNEKLGFEGKEEGIVAQAVVLIYRAEAAEQD
HHHHHCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNWMDIRIGQGYDVHALVEGRKLILGGVEIPHTRGLLGHSDADALLHAITDALFGAAGLG
CCCEEEEECCCCCEEEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
DIGRHFPDTDPAFAGADSRVLLREAVRRVHKAGYAVGNVDATVIAQKPKLAPHVPGMVAN
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECCCCEEEEECCCCCCCCCCHHHHH
LAEDLGIAPERCNVKAKTNEKLGFEGKEEGIVAQAVVLIYRAEAAEQD
HHHHHCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA