Click here to switch to the map view.

The map label for this gene is ispD [H]

Identifier: 187928983

GI number: 187928983

Start: 2004529

End: 2005287

Strand: Reverse

Name: ispD [H]

Synonym: Rpic_1903

Alternate gene names: 187928983

Gene position: 2005287-2004529 (Counterclockwise)

Preceding gene: 187928988

Following gene: 187928982

Centisome position: 50.86

GC content: 67.72

Gene sequence:

>759_bases
ATGTCGATTTCACACCCTGGCCGACGCCGTTTTGCGCTGATTCCCTCGGCCGGCACCGGCTCCCGCGCCGGCGGCGATCT
GCCCAAGCAATATCAGGTGATTGCCGGGCGGCCGATGTTGTGGCACACGGCGCAGGCATTCCTGGCCAGCCCCGAAATCG
ACACCGTGCTGGTCGTGACAACGCCCGGCGCACAGCCGCTCGTGCAGCGCTTTCCCGAGGGCGGTTTCAACGCGCCCAAG
CTGGTGGAGGTCGATTGCGGCGGCGACTCGCGCCACGCATCCGTCACGCATGGGCTGGCGGCGTTGCGTGGTCTCGGTGC
GCACGATGACGACTGGGTGCTCGTGCATGATGCGGCGCGTCCGGGCCTGACGCCCGCACTGGTCGCGCGTTTGGTCGCGG
CCGTGGAGGCCGGAGGCACGCAAGCTGCGGGAGGCATCCTTGCGCTACCCGTGGCAGATACGCTCAAACGTGCCGATGCG
GCTCAGCACATCAACGCCACCGTGCCGCGCGATGGCCTGTGGCAAGCGCAGACGCCGCAGATGTTTCCGCTTGGCCTGCT
GGAGCGCGCCTTGCGGGAGGCGCTGTTGCAACAGCGCATCGTCACCGACGAAGCCAGCGCCATTGAAGCCGCCGGTCACC
CGCCGCTGCTTGTGCCGGGGGCGCTGCGCAACTTCAAGGTGACGTATTCCGACGATTTCGCACTCGCCGAGGCCATCCTC
GCGCAGCGCGCACAACCCAACGAGGCATCGCAAGCATGA

Upstream 100 bases:

>100_bases
TCGCGACGCAGAGCGCTATTATAAAATCCGCGCCAGCCGCGCGCTTGGGCGGGCCAAAACCCACGCAAATGCTGCGCCCA
CGTTCACTTTCCCGCCTCTC

Downstream 100 bases:

>100_bases
ACTGGATGGACATCCGCATTGGTCAAGGCTATGACGTGCACGCGCTGGTGGAGGGCCGCAAGCTGATCCTGGGCGGCGTG
GAGATTCCGCACACACGCGG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSISHPGRRRFALIPSAGTGSRAGGDLPKQYQVIAGRPMLWHTAQAFLASPEIDTVLVVTTPGAQPLVQRFPEGGFNAPK
LVEVDCGGDSRHASVTHGLAALRGLGAHDDDWVLVHDAARPGLTPALVARLVAAVEAGGTQAAGGILALPVADTLKRADA
AQHINATVPRDGLWQAQTPQMFPLGLLERALREALLQQRIVTDEASAIEAAGHPPLLVPGALRNFKVTYSDDFALAEAIL
AQRAQPNEASQA

Sequences:

>Translated_252_residues
MSISHPGRRRFALIPSAGTGSRAGGDLPKQYQVIAGRPMLWHTAQAFLASPEIDTVLVVTTPGAQPLVQRFPEGGFNAPK
LVEVDCGGDSRHASVTHGLAALRGLGAHDDDWVLVHDAARPGLTPALVARLVAAVEAGGTQAAGGILALPVADTLKRADA
AQHINATVPRDGLWQAQTPQMFPLGLLERALREALLQQRIVTDEASAIEAAGHPPLLVPGALRNFKVTYSDDFALAEAIL
AQRAQPNEASQA
>Mature_251_residues
SISHPGRRRFALIPSAGTGSRAGGDLPKQYQVIAGRPMLWHTAQAFLASPEIDTVLVVTTPGAQPLVQRFPEGGFNAPKL
VEVDCGGDSRHASVTHGLAALRGLGAHDDDWVLVHDAARPGLTPALVARLVAAVEAGGTQAAGGILALPVADTLKRADAA
QHINATVPRDGLWQAQTPQMFPLGLLERALREALLQQRIVTDEASAIEAAGHPPLLVPGALRNFKVTYSDDFALAEAILA
QRAQPNEASQA

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=257, Percent_Identity=27.2373540856031, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789104, Length=232, Percent_Identity=45.6896551724138, Blast_Score=160, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 26459; Mature: 26328

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISHPGRRRFALIPSAGTGSRAGGDLPKQYQVIAGRPMLWHTAQAFLASPEIDTVLVVT
CCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHEECCCCHHHHHHHHHHCCCCCCEEEEEE
TPGAQPLVQRFPEGGFNAPKLVEVDCGGDSRHASVTHGLAALRGLGAHDDDWVLVHDAAR
CCCCCHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCC
PGLTPALVARLVAAVEAGGTQAAGGILALPVADTLKRADAAQHINATVPRDGLWQAQTPQ
CCCCHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MFPLGLLERALREALLQQRIVTDEASAIEAAGHPPLLVPGALRNFKVTYSDDFALAEAIL
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEECCCCCCCEEEECCCHHHHHHHH
AQRAQPNEASQA
HHCCCCCCCCCC
>Mature Secondary Structure 
SISHPGRRRFALIPSAGTGSRAGGDLPKQYQVIAGRPMLWHTAQAFLASPEIDTVLVVT
CCCCCCCCEEEEEECCCCCCCCCCCCCHHHHEECCCCHHHHHHHHHHCCCCCCEEEEEE
TPGAQPLVQRFPEGGFNAPKLVEVDCGGDSRHASVTHGLAALRGLGAHDDDWVLVHDAAR
CCCCCHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCC
PGLTPALVARLVAAVEAGGTQAAGGILALPVADTLKRADAAQHINATVPRDGLWQAQTPQ
CCCCHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MFPLGLLERALREALLQQRIVTDEASAIEAAGHPPLLVPGALRNFKVTYSDDFALAEAIL
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEECCCCCCCEEEECCCHHHHHHHH
AQRAQPNEASQA
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]