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The map label for this gene is tesA [H]

Identifier: 187928944

GI number: 187928944

Start: 1961306

End: 1961923

Strand: Direct

Name: tesA [H]

Synonym: Rpic_1863

Alternate gene names: 187928944

Gene position: 1961306-1961923 (Clockwise)

Preceding gene: 187928942

Following gene: 187928945

Centisome position: 49.75

GC content: 65.05

Gene sequence:

>618_bases
ATGTTCAGCATGCTGCCGCTCGCACAGGCCGCTGCAGCAGAAGGCGCGCCGGCATCGCATCGTGTGGTGGTGCTGGGTGA
CAGCCTGTCGGCGGGATACGGCCTGGCGCAAGGCACAGGCTGGGTCGCGTTGCTCGACAGGCGCCTCAAAGAACGGAAGC
CCGATTATAGCGTCGCCAATGCAAGCATCAGCGGCGACACCACGGCCGGCGGCCGCTCGCGCTTGCCCGCCGTGCTGGCC
CGCGAAAAGCCCTCTGTCGTGATTCTCGAACTGGGCGGTAACGACGCGCTGCGCGGCCTGTCGCTCGCCTCCAGCGAGGG
CAACATGAAGGCGATGATCGAAGCATCGCAAGCGGCTGGCGCGAAGGTGCTGCTGGTGGGCATGCGCATCCCGCCGAACT
ACGGCCCGGATTACAGCGAGCGCTTCTTCGCCATGTTCGGCAAGCTGGCGCAGCAGTACAAGCTGCCACTGGTGCCATTC
CTGCTCGAAGGGGTTGCGCAGCGGCCCGACTGGTTCCAGGAAGATCGCATCCACCCCATTGCCGCAGCGCAGCCGACGAT
GCTCGATAACGTGTGGCCCAAACTGGAACCTCTCCTTAAAGCCCGGGTCGGAAGATGA

Upstream 100 bases:

>100_bases
ATCGACTTCAATCACCCAGGTAGCGGAAGACGACATGATTGGACTGGTAGGTAGTAGAAGGCGCAGTGTGAAGTCACTAG
CAGCGGCACTAGGCTTCGCC

Downstream 100 bases:

>100_bases
CCCTTCTGCCGCACCGATGGCGTCATCCGGCGCATCGGATTTCCAGCTCCGGCACGGCGGCCACCTCTCACTCGGGCGAA
CACCATGAAGCTTCTGGCAA

Product: Lysophospholipase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MFSMLPLAQAAAAEGAPASHRVVVLGDSLSAGYGLAQGTGWVALLDRRLKERKPDYSVANASISGDTTAGGRSRLPAVLA
REKPSVVILELGGNDALRGLSLASSEGNMKAMIEASQAAGAKVLLVGMRIPPNYGPDYSERFFAMFGKLAQQYKLPLVPF
LLEGVAQRPDWFQEDRIHPIAAAQPTMLDNVWPKLEPLLKARVGR

Sequences:

>Translated_205_residues
MFSMLPLAQAAAAEGAPASHRVVVLGDSLSAGYGLAQGTGWVALLDRRLKERKPDYSVANASISGDTTAGGRSRLPAVLA
REKPSVVILELGGNDALRGLSLASSEGNMKAMIEASQAAGAKVLLVGMRIPPNYGPDYSERFFAMFGKLAQQYKLPLVPF
LLEGVAQRPDWFQEDRIHPIAAAQPTMLDNVWPKLEPLLKARVGR
>Mature_205_residues
MFSMLPLAQAAAAEGAPASHRVVVLGDSLSAGYGLAQGTGWVALLDRRLKERKPDYSVANASISGDTTAGGRSRLPAVLA
REKPSVVILELGGNDALRGLSLASSEGNMKAMIEASQAAGAKVLLVGMRIPPNYGPDYSERFFAMFGKLAQQYKLPLVPF
LLEGVAQRPDWFQEDRIHPIAAAQPTMLDNVWPKLEPLLKARVGR

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=185, Percent_Identity=43.2432432432432, Blast_Score=159, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 21966; Mature: 21966

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU ; PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSMLPLAQAAAAEGAPASHRVVVLGDSLSAGYGLAQGTGWVALLDRRLKERKPDYSVAN
CCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEC
ASISGDTTAGGRSRLPAVLAREKPSVVILELGGNDALRGLSLASSEGNMKAMIEASQAAG
CCCCCCCCCCCCCCCCHHEECCCCCEEEEEECCCCHHHCEEEECCCCCEEEEEECCCCCC
AKVLLVGMRIPPNYGPDYSERFFAMFGKLAQQYKLPLVPFLLEGVAQRPDWFQEDRIHPI
CEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHCCCCCC
AAAQPTMLDNVWPKLEPLLKARVGR
CCCCCHHHHHHCHHHHHHHHHHCCC
>Mature Secondary Structure
MFSMLPLAQAAAAEGAPASHRVVVLGDSLSAGYGLAQGTGWVALLDRRLKERKPDYSVAN
CCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEC
ASISGDTTAGGRSRLPAVLAREKPSVVILELGGNDALRGLSLASSEGNMKAMIEASQAAG
CCCCCCCCCCCCCCCCHHEECCCCCEEEEEECCCCHHHCEEEECCCCCEEEEEECCCCCC
AKVLLVGMRIPPNYGPDYSERFFAMFGKLAQQYKLPLVPFLLEGVAQRPDWFQEDRIHPI
CEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHCCCCCC
AAAQPTMLDNVWPKLEPLLKARVGR
CCCCCHHHHHHCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]