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The map label for this gene is lepB [H]

Identifier: 187928945

GI number: 187928945

Start: 1962008

End: 1962700

Strand: Direct

Name: lepB [H]

Synonym: Rpic_1864

Alternate gene names: 187928945

Gene position: 1962008-1962700 (Clockwise)

Preceding gene: 187928944

Following gene: 187928959

Centisome position: 49.76

GC content: 64.36

Gene sequence:

>693_bases
ATGAAGCTTCTGGCAAGCGGGCTGCGCGCAGCGCGTGACAACAAGCGGTTCCTGGTCGGCATGTCGCTGCTTTTTGCCTT
CCGCGCCTGCATTGCGGACTGGGCAGTCGTGCCGAGCGGCTCAATGAATCCCACGCTCATCGAAGGCGACTACATCTTGA
TGAACCGGTTGGCTTACGGGGTGCGCGTACCGGCCACCACCGTCTGGCTCAAGCGCGGCAGCGACCCCCGACGTGGTGAT
GTCGTCGTCTTCTCCTCGCCCGAAAACGGCACGAAGCTGGTCAAGCGGCTCATCGGGCTGCCCGGCGACGTGGTTGAAAT
GCGTGACGAAGCGCTCTACATCAATCACCGACGCATGGCCTATGCGCCGCTGCCAGACGTCGCACCCGGCGCGCTGCCGC
AAGCAACGGCGATGCTGCCGCACGAACTCCGGTCGGAAGCGCTACCGGGCCGGGAGCACACCGTGATGGTCTTGCCGGAG
GTGCGTGCCCTGCGCAGTTTCGGTCCCATCACCGTCCCGCAAGACCACTACCTCATGCTGGGTGACAACCGCGACAACAG
CCGCGACTCCCGCTACTTCGGCCTCGTACCGCGCAAGAATCTGATCGCCCGCGCTTCCCATGTGGCCCTATCGTTCGATC
CAGACCACTTGTACATGCCGCGCCTCGCACGCATGGGCAAGCCGCTGGACTAG

Upstream 100 bases:

>100_bases
CCGGGTCGGAAGATGACCCTTCTGCCGCACCGATGGCGTCATCCGGCGCATCGGATTTCCAGCTCCGGCACGGCGGCCAC
CTCTCACTCGGGCGAACACC

Downstream 100 bases:

>100_bases
CGCAACGCCAAAAAGTACGCAAAGAAAAAGCCCCCTCGTTATTGAGGGGGCTTTTTCGTCAGGCTACGTGCTCTGCACGC
CGTATCAGTCCTCTGCCGTA

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKLLASGLRAARDNKRFLVGMSLLFAFRACIADWAVVPSGSMNPTLIEGDYILMNRLAYGVRVPATTVWLKRGSDPRRGD
VVVFSSPENGTKLVKRLIGLPGDVVEMRDEALYINHRRMAYAPLPDVAPGALPQATAMLPHELRSEALPGREHTVMVLPE
VRALRSFGPITVPQDHYLMLGDNRDNSRDSRYFGLVPRKNLIARASHVALSFDPDHLYMPRLARMGKPLD

Sequences:

>Translated_230_residues
MKLLASGLRAARDNKRFLVGMSLLFAFRACIADWAVVPSGSMNPTLIEGDYILMNRLAYGVRVPATTVWLKRGSDPRRGD
VVVFSSPENGTKLVKRLIGLPGDVVEMRDEALYINHRRMAYAPLPDVAPGALPQATAMLPHELRSEALPGREHTVMVLPE
VRALRSFGPITVPQDHYLMLGDNRDNSRDSRYFGLVPRKNLIARASHVALSFDPDHLYMPRLARMGKPLD
>Mature_230_residues
MKLLASGLRAARDNKRFLVGMSLLFAFRACIADWAVVPSGSMNPTLIEGDYILMNRLAYGVRVPATTVWLKRGSDPRRGD
VVVFSSPENGTKLVKRLIGLPGDVVEMRDEALYINHRRMAYAPLPDVAPGALPQATAMLPHELRSEALPGREHTVMVLPE
VRALRSFGPITVPQDHYLMLGDNRDNSRDSRYFGLVPRKNLIARASHVALSFDPDHLYMPRLARMGKPLD

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=253, Percent_Identity=30.0395256916996, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 25625; Mature: 25625

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS00501 SPASE_I_1 ; PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLASGLRAARDNKRFLVGMSLLFAFRACIADWAVVPSGSMNPTLIEGDYILMNRLAYG
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCEECCCCCCCCEEEECCHHEEHHHHHC
VRVPATTVWLKRGSDPRRGDVVVFSSPENGTKLVKRLIGLPGDVVEMRDEALYINHRRMA
EECCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHCCCEEEEECCEEE
YAPLPDVAPGALPQATAMLPHELRSEALPGREHTVMVLPEVRALRSFGPITVPQDHYLML
ECCCCCCCCCCCCHHHHHCCHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCCCCCCEEEE
GDNRDNSRDSRYFGLVPRKNLIARASHVALSFDPDHLYMPRLARMGKPLD
ECCCCCCCCCEEEECCCCHHHHHHHHEEEEEECCCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MKLLASGLRAARDNKRFLVGMSLLFAFRACIADWAVVPSGSMNPTLIEGDYILMNRLAYG
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCEECCCCCCCCEEEECCHHEEHHHHHC
VRVPATTVWLKRGSDPRRGDVVVFSSPENGTKLVKRLIGLPGDVVEMRDEALYINHRRMA
EECCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHCCCEEEEECCEEE
YAPLPDVAPGALPQATAMLPHELRSEALPGREHTVMVLPEVRALRSFGPITVPQDHYLML
ECCCCCCCCCCCCHHHHHCCHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCCCCCCEEEE
GDNRDNSRDSRYFGLVPRKNLIARASHVALSFDPDHLYMPRLARMGKPLD
ECCCCCCCCCEEEECCCCHHHHHHHHEEEEEECCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1546969 [H]