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The map label for this gene is hofB [H]

Identifier: 187928620

GI number: 187928620

Start: 1628656

End: 1630242

Strand: Direct

Name: hofB [H]

Synonym: Rpic_1536

Alternate gene names: 187928620

Gene position: 1628656-1630242 (Clockwise)

Preceding gene: 187928619

Following gene: 187928621

Centisome position: 41.31

GC content: 60.62

Gene sequence:

>1587_bases
ATGGTGGCTTTGGTTGAGCGGTTCAGGGCATTCTCGCGCGTTGACGCGCATGTCGAGCCAATGCGGCTCCCGCCTGTTCC
TGCCACGCTGATGCAACACGTCGTGTGGGCGGGGGTCGGCGAGAAGATTCAGGTCGATGGCCGCTATGCAGGTGGTTCGG
CCTTTCTCACATGGTTGGCTGACCTCAAACGCCATGGTCTGGAGCCGGTTGTTGAATCTCTTGCCCCGCAGGAGCTGGCG
CAAGCGCGCGAAAGCACGTCGGACGGGCAATCGGGCGACGCATACCTTGAGTTTCTGGAAATGTCGCGCCGGCGGCACGT
CGAGGCTGCCGAACTGGGGGCGTCCGACCTTCACGTTCTGCAGCGAGAAGACCATGCTGAATTTCAGTTGCGCATTGACG
GCGAGCTATGGACGGTGCCCGAGTGGGAACTGAACGCTGCGCAGGGGGAAGCTTTTGTGCGAGCAACTTGCGTCGGCCTC
GCAACCGTCAAAGAGCCGACATTCAATCCGCTCGAGTTCCAGGAAGCGCAGATCAGCGGCAACAAGCTGCCTGAGACCGA
TATTGAAAGTGTTCGCATTACGCGCGGCCCAGCCTATCCGATCGAGGCCGGTGGTGGCGTCATGGTTAATCGCCTGCAAC
CGCGCAGGCACACGGCCACACTTCAGCGACGCGAGACGCCGGCGAGTCGTCTGCGACTGGTGAGACCGGAGGCGCCGGCC
GGCAAAGTCGACTTTGAGCGGATGGGATTCACGACGCTGCAGCTCGCGATGTTCGAGCAGGTCGTCCTGATGCCGGATGG
TCTGTTTATTCAGACGGGGCCGACGGGAAGTGGCAAGACCACGACCGGCTACGAAATGCTGCGCTACAAGGGGCAGATTT
CGCCCGAGCTGCGGCAGATCACCCGTGAGCATCCGGTCGAGTACCCGCGTGAATGGGCAGTGCAACTGCTTTCGACGGGG
CAGAACTCCTCATACCTCGTCGAACGCATGCTCCGGATGGACCCGGACATCATCGACATCGGCGAGATCCGCACAGGCAA
TGAAGGTGTTGCAGCAGTTCAGGCAGCGCAGACCGGGCACTTGGTCTTCGGCTCGCTCCACGTTCTTGATCCGTTCGAAC
TGATTGGTCGCCTGCAAATGCTCGACCACACGCTGCTTTCCAAGGAGTTGATGTGCAACCACAAAATCATCGCGGGTTTC
ATGGGGCAGCGGATGGTTCCCGTGCTGTGCACGGAGTGTCGGGAGCCATTGGCCAAACATCTCGACGCAATGCCGGGAAT
TCTGCTCGACCGCATCAAAACCTGGGGAAACATCGAACAGGTCCATGTGCGGGGAAAGGGATGTCCGCACTGCTTCGGCC
GGCAAATCCGCGGGCGTGAGGCAGTTGCAGAGGTTGTCCTTAGCACCGAAGAGCTGATGGAAGACTTCCTCAGGGGGACG
TCGTTGGCACGTCGAAATCACCGCCTGCGTGAGGGCTCCGACAAATCCATGCTCGGCAATGTCATGGAACGTGTGTTGGC
CGGCAGGATCGATCCGCGCGACGCGCAGAAGAACGTGCCCATTGAGGCGTATCGCGAGGGCGTATGA

Upstream 100 bases:

>100_bases
GTCAGCGTCGACCTGCGAGATGGCCAGTGGCATGCGAAAGGGACGCTCTACGTTAACCGTTTTCCTGGGGCGACTGCTGC
CCTCAAGGTGGGGAGCTGAC

Downstream 100 bases:

>100_bases
ATCTCCAGAAAGCTCTGCAGTGGCTGAAGGGCAATCCTCTGCCATGGAAGCTCCGAAAACGCCTCTATGAACGTGCGTCA
ACGCATCTGGCGAACGACGT

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MVALVERFRAFSRVDAHVEPMRLPPVPATLMQHVVWAGVGEKIQVDGRYAGGSAFLTWLADLKRHGLEPVVESLAPQELA
QARESTSDGQSGDAYLEFLEMSRRRHVEAAELGASDLHVLQREDHAEFQLRIDGELWTVPEWELNAAQGEAFVRATCVGL
ATVKEPTFNPLEFQEAQISGNKLPETDIESVRITRGPAYPIEAGGGVMVNRLQPRRHTATLQRRETPASRLRLVRPEAPA
GKVDFERMGFTTLQLAMFEQVVLMPDGLFIQTGPTGSGKTTTGYEMLRYKGQISPELRQITREHPVEYPREWAVQLLSTG
QNSSYLVERMLRMDPDIIDIGEIRTGNEGVAAVQAAQTGHLVFGSLHVLDPFELIGRLQMLDHTLLSKELMCNHKIIAGF
MGQRMVPVLCTECREPLAKHLDAMPGILLDRIKTWGNIEQVHVRGKGCPHCFGRQIRGREAVAEVVLSTEELMEDFLRGT
SLARRNHRLREGSDKSMLGNVMERVLAGRIDPRDAQKNVPIEAYREGV

Sequences:

>Translated_528_residues
MVALVERFRAFSRVDAHVEPMRLPPVPATLMQHVVWAGVGEKIQVDGRYAGGSAFLTWLADLKRHGLEPVVESLAPQELA
QARESTSDGQSGDAYLEFLEMSRRRHVEAAELGASDLHVLQREDHAEFQLRIDGELWTVPEWELNAAQGEAFVRATCVGL
ATVKEPTFNPLEFQEAQISGNKLPETDIESVRITRGPAYPIEAGGGVMVNRLQPRRHTATLQRRETPASRLRLVRPEAPA
GKVDFERMGFTTLQLAMFEQVVLMPDGLFIQTGPTGSGKTTTGYEMLRYKGQISPELRQITREHPVEYPREWAVQLLSTG
QNSSYLVERMLRMDPDIIDIGEIRTGNEGVAAVQAAQTGHLVFGSLHVLDPFELIGRLQMLDHTLLSKELMCNHKIIAGF
MGQRMVPVLCTECREPLAKHLDAMPGILLDRIKTWGNIEQVHVRGKGCPHCFGRQIRGREAVAEVVLSTEELMEDFLRGT
SLARRNHRLREGSDKSMLGNVMERVLAGRIDPRDAQKNVPIEAYREGV
>Mature_528_residues
MVALVERFRAFSRVDAHVEPMRLPPVPATLMQHVVWAGVGEKIQVDGRYAGGSAFLTWLADLKRHGLEPVVESLAPQELA
QARESTSDGQSGDAYLEFLEMSRRRHVEAAELGASDLHVLQREDHAEFQLRIDGELWTVPEWELNAAQGEAFVRATCVGL
ATVKEPTFNPLEFQEAQISGNKLPETDIESVRITRGPAYPIEAGGGVMVNRLQPRRHTATLQRRETPASRLRLVRPEAPA
GKVDFERMGFTTLQLAMFEQVVLMPDGLFIQTGPTGSGKTTTGYEMLRYKGQISPELRQITREHPVEYPREWAVQLLSTG
QNSSYLVERMLRMDPDIIDIGEIRTGNEGVAAVQAAQTGHLVFGSLHVLDPFELIGRLQMLDHTLLSKELMCNHKIIAGF
MGQRMVPVLCTECREPLAKHLDAMPGILLDRIKTWGNIEQVHVRGKGCPHCFGRQIRGREAVAEVVLSTEELMEDFLRGT
SLARRNHRLREGSDKSMLGNVMERVLAGRIDPRDAQKNVPIEAYREGV

Specific function: Unknown

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=410, Percent_Identity=28.780487804878, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789723, Length=410, Percent_Identity=28.0487804878049, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082188, Length=154, Percent_Identity=32.4675324675325, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 59050; Mature: 59050

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVALVERFRAFSRVDAHVEPMRLPPVPATLMQHVVWAGVGEKIQVDGRYAGGSAFLTWLA
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCEECCHHHHHHHHH
DLKRHGLEPVVESLAPQELAQARESTSDGQSGDAYLEFLEMSRRRHVEAAELGASDLHVL
HHHHCCHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHH
QREDHAEFQLRIDGELWTVPEWELNAAQGEAFVRATCVGLATVKEPTFNPLEFQEAQISG
HCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCC
NKLPETDIESVRITRGPAYPIEAGGGVMVNRLQPRRHTATLQRRETPASRLRLVRPEAPA
CCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHEEECCCCCC
GKVDFERMGFTTLQLAMFEQVVLMPDGLFIQTGPTGSGKTTTGYEMLRYKGQISPELRQI
CCCCHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHH
TREHPVEYPREWAVQLLSTGQNSSYLVERMLRMDPDIIDIGEIRTGNEGVAAVQAAQTGH
HHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCHHEEECCCCCC
LVFGSLHVLDPFELIGRLQMLDHTLLSKELMCNHKIIAGFMGQRMVPVLCTECREPLAKH
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHH
LDAMPGILLDRIKTWGNIEQVHVRGKGCPHCFGRQIRGREAVAEVVLSTEELMEDFLRGT
HHHCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SLARRNHRLREGSDKSMLGNVMERVLAGRIDPRDAQKNVPIEAYREGV
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MVALVERFRAFSRVDAHVEPMRLPPVPATLMQHVVWAGVGEKIQVDGRYAGGSAFLTWLA
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCEECCHHHHHHHHH
DLKRHGLEPVVESLAPQELAQARESTSDGQSGDAYLEFLEMSRRRHVEAAELGASDLHVL
HHHHCCHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHH
QREDHAEFQLRIDGELWTVPEWELNAAQGEAFVRATCVGLATVKEPTFNPLEFQEAQISG
HCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCC
NKLPETDIESVRITRGPAYPIEAGGGVMVNRLQPRRHTATLQRRETPASRLRLVRPEAPA
CCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHEEECCCCCC
GKVDFERMGFTTLQLAMFEQVVLMPDGLFIQTGPTGSGKTTTGYEMLRYKGQISPELRQI
CCCCHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHH
TREHPVEYPREWAVQLLSTGQNSSYLVERMLRMDPDIIDIGEIRTGNEGVAAVQAAQTGH
HHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCHHEEECCCCCC
LVFGSLHVLDPFELIGRLQMLDHTLLSKELMCNHKIIAGFMGQRMVPVLCTECREPLAKH
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHH
LDAMPGILLDRIKTWGNIEQVHVRGKGCPHCFGRQIRGREAVAEVVLSTEELMEDFLRGT
HHHCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SLARRNHRLREGSDKSMLGNVMERVLAGRIDPRDAQKNVPIEAYREGV
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959070; 8202364; 9278503 [H]